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Q8R4T9

- UT2_MOUSE

UniProt

Q8R4T9 - UT2_MOUSE

Protein

Urea transporter 2

Gene

Slc14a2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 2 (23 Mar 2010)
      Previous versions | rss
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    Functioni

    Specialized low-affinity vasopressin-regulated urea transporter. Mediates rapid transepithelial urea transport across the inner medullary collecting duct and plays a major role in the urinary concentrating mechanism. Vasopressin regulates urea transport by increasing isoform A1 accumulation in the plasma membrane and/or phosphorylation of isoform A1 By similarity.By similarity

    GO - Molecular functioni

    1. cell adhesion molecule binding Source: MGI
    2. urea transmembrane transporter activity Source: MGI

    Enzyme and pathway databases

    ReactomeiREACT_217615. Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Urea transporter 2
    Alternative name(s):
    Solute carrier family 14 member 2
    Urea transporter, kidney
    Gene namesi
    Name:Slc14a2
    Synonyms:Ut2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:1351653. Slc14a2.

    Subcellular locationi

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 930930Urea transporter 2PRO_0000392531Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei487 – 4871PhosphoserineBy similarity
    Glycosylationi743 – 7431N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ8R4T9.
    PRIDEiQ8R4T9.

    PTM databases

    PhosphoSiteiQ8R4T9.

    Expressioni

    Tissue specificityi

    Highly expressed in kidney medulla. Also detected at the protein level in testes, heart, brain and liver. In the kidney, present in thin descending limbs of the loop of Henle and in the middle and terminal inner medullary collecting ducts. Isoform A5 is expressed in the peritubular myoid cells forming the outermost layer of the seminiferous tubules within the testes and is not detected in kidney. Isoform A5 mRNA levels are coordinated with the stage of testes development and increase 15 days postpartum, commensurate with the start of seminiferous tubule fluid movement.1 Publication

    Gene expression databases

    BgeeiQ8R4T9.
    GenevestigatoriQ8R4T9.

    Interactioni

    Subunit structurei

    Isoform A1 interacts with SNAPIN which may be important for recruitment to the plasma membrane.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000025434.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8R4T9.
    SMRiQ8R4T9. Positions 106-450, 569-910.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei145 – 16521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei185 – 20521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei213 – 23321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei242 – 26221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei280 – 30021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei311 – 33121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei350 – 37223HelicalSequence AnalysisAdd
    BLAST
    Transmembranei401 – 42121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei610 – 63021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei648 – 66821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei676 – 69621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei705 – 72521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei774 – 79421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei813 – 83321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei842 – 86221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei864 – 88421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the urea transporter family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG4413.
    GeneTreeiENSGT00390000018729.
    HOGENOMiHOG000168437.
    HOVERGENiHBG073255.
    InParanoidiQ8R4T9.
    KOiK08716.
    PhylomeDBiQ8R4T9.

    Family and domain databases

    Gene3Di1.10.3430.10. 2 hits.
    InterProiIPR029020. Ammonium/urea_transptr.
    IPR004937. Urea_transporter.
    [Graphical view]
    PANTHERiPTHR10464. PTHR10464. 1 hit.
    PfamiPF03253. UT. 2 hits.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform A1 (identifier: Q8R4T9-1) [UniParc]FASTAAdd to Basket

    Also known as: UTA-1

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSDHHPLKEM SDSNSSPLLP EPLSSRYKLY ESELSSPTWP SSSQDTHPAL    50
    PLLEMPEEKD LRSSDEDSHI VKIEKPNERN KRRESEVSRR ASAGRGGFSL 100
    FQAVSYLTGD MKECKNWLKD KPLVLQFLDW VLRGAAQVMF VNNPISGLII 150
    FIGLLIQNPW WTIAGTLGTV ASTLAALALS QDRSAIASGL HGYNGMLVGL 200
    LMAVFSEKLD YYWWLLFPVT FTSMACPIIS SALSTIFAKW DLPVFTLPFN 250
    IALTLYLAAT GHYNLFFPTT LIKPASAAPN ITWTEIEMPL LLQTIPVGVG 300
    QVYGCDNPWT GGVILVALFI SSPLICLHAA IGSIVGLLAA LTVATPFETI 350
    YLGLWSYNCV LSCIAIGGMF YALTWQTHLL ALVCALFCAY MGAALSNTMA 400
    VVGVPSGTWA FCLSTLTFLL LTSNNTGIYK LPLSKVTYPE ANRIYFLTVR 450
    RSEEEKSPNG GSGEQSHGSG QWKAEESSET VLPRRRSVFH IEWSSIRRRS 500
    KVFGKGEHQE RQTKEPLPCP YRKPTVELFD LDTMEESTEI KVEANTARTS 550
    WIQSSMVAGG KRVSKALSYI TGEMKECGEG LKDKSPVFQF LDWVLRGMSQ 600
    VMFVNNPLSG ILIVLGLFVQ NPWWAISGCL GTVMSTLTAL ILSQDKSAIA 650
    AGLHGYNGVL VGLLMAVFSD KGNYYWWLLL PVIVMSMTCP ILSSALSTVF 700
    SKWDLPVFTL PFNIAVTLYL AATGHHNLFF PTTLLQPATT TPNITWSDIQ 750
    VSLLLRAIPV GIGQVYGCDN PWTGGIFLVA LFISSPLICL HAAIGSTIGM 800
    LAALSIATPF DSIYFGLCGF NSTLACIAIG GMFYVITWQT HLLAIACALF 850
    AAYLGAALAN MLSVFGLPPC TWPFCLSALT FLLLTSNNPA IYKLPLSKVT 900
    YPEANRIYFL SQEKNRRAST ITKYQAYDVS 930
    Length:930
    Mass (Da):102,047
    Last modified:March 23, 2010 - v2
    Checksum:i298727BAFBE66D46
    GO
    Isoform A2 (identifier: Q8R4T9-2) [UniParc]FASTAAdd to Basket

    Also known as: UTA-2

    The sequence of this isoform differs from the canonical sequence as follows:
         1-533: Missing.

    Show »
    Length:397
    Mass (Da):43,116
    Checksum:i4E24BE4C44956F4B
    GO
    Isoform A3 (identifier: Q8R4T9-3) [UniParc]FASTAAdd to Basket

    Also known as: UTA-3

    The sequence of this isoform differs from the canonical sequence as follows:
         461-461: G → D
         462-930: Missing.

    Show »
    Length:461
    Mass (Da):50,615
    Checksum:i18B0F3A3B6B18030
    GO
    Isoform A5 (identifier: Q8R4T9-4) [UniParc]FASTAAdd to Basket

    Also known as: UTA-5

    The sequence of this isoform differs from the canonical sequence as follows:
         1-138: Missing.
         461-461: G → D
         462-930: Missing.

    Note: Implicated in the urea movement across the blood-testis barrier. Does not translocate water. Expressed in testis, not expressed in kidney.

    Show »
    Length:323
    Mass (Da):34,921
    Checksum:i6B9774A12E42D609
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti313 – 3131V → M in AAG32167. (PubMed:11029290)Curated
    Sequence conflicti313 – 3131V → M in AAG32168. (PubMed:11029290)Curated
    Sequence conflicti313 – 3131V → M in AAM00357. (PubMed:11880324)Curated
    Sequence conflicti313 – 3131V → M in EDL09434. 1 PublicationCurated
    Sequence conflicti538 – 5381T → A in AAI50681. (PubMed:15489334)Curated
    Sequence conflicti898 – 8981K → R in AAM00357. (PubMed:11880324)Curated
    Sequence conflicti898 – 8981K → R in AAM21206. (PubMed:12217874)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 533533Missing in isoform A2. 2 PublicationsVSP_038810Add
    BLAST
    Alternative sequencei1 – 138138Missing in isoform A5. 1 PublicationVSP_038809Add
    BLAST
    Alternative sequencei461 – 4611G → D in isoform A3 and isoform A5. 1 PublicationVSP_038811
    Alternative sequencei462 – 930469Missing in isoform A3 and isoform A5. 1 PublicationVSP_038812Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF258601 mRNA. Translation: AAG32167.1.
    AF258602 mRNA. Translation: AAG32168.1.
    AF366052 mRNA. Translation: AAM00357.1.
    AF367359 mRNA. Translation: AAM21206.1.
    CH466528 Genomic DNA. Translation: EDL09434.1.
    BC150680 mRNA. Translation: AAI50681.1.
    CCDSiCCDS29361.1. [Q8R4T9-1]
    CCDS50331.1. [Q8R4T9-4]
    RefSeqiNP_001103744.1. NM_001110274.1.
    NP_109608.1. NM_030683.3.
    UniGeneiMm.44158.

    Genome annotation databases

    EnsembliENSMUST00000163367; ENSMUSP00000126416; ENSMUSG00000024552.
    GeneIDi27411.
    KEGGimmu:27411.
    UCSCiuc008fsh.2. mouse. [Q8R4T9-3]
    uc012bfh.1. mouse. [Q8R4T9-4]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF258601 mRNA. Translation: AAG32167.1 .
    AF258602 mRNA. Translation: AAG32168.1 .
    AF366052 mRNA. Translation: AAM00357.1 .
    AF367359 mRNA. Translation: AAM21206.1 .
    CH466528 Genomic DNA. Translation: EDL09434.1 .
    BC150680 mRNA. Translation: AAI50681.1 .
    CCDSi CCDS29361.1. [Q8R4T9-1 ]
    CCDS50331.1. [Q8R4T9-4 ]
    RefSeqi NP_001103744.1. NM_001110274.1.
    NP_109608.1. NM_030683.3.
    UniGenei Mm.44158.

    3D structure databases

    ProteinModelPortali Q8R4T9.
    SMRi Q8R4T9. Positions 106-450, 569-910.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000025434.

    PTM databases

    PhosphoSitei Q8R4T9.

    Proteomic databases

    PaxDbi Q8R4T9.
    PRIDEi Q8R4T9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000163367 ; ENSMUSP00000126416 ; ENSMUSG00000024552 .
    GeneIDi 27411.
    KEGGi mmu:27411.
    UCSCi uc008fsh.2. mouse. [Q8R4T9-3 ]
    uc012bfh.1. mouse. [Q8R4T9-4 ]

    Organism-specific databases

    CTDi 8170.
    MGIi MGI:1351653. Slc14a2.

    Phylogenomic databases

    eggNOGi COG4413.
    GeneTreei ENSGT00390000018729.
    HOGENOMi HOG000168437.
    HOVERGENi HBG073255.
    InParanoidi Q8R4T9.
    KOi K08716.
    PhylomeDBi Q8R4T9.

    Enzyme and pathway databases

    Reactomei REACT_217615. Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds.

    Miscellaneous databases

    NextBioi 305432.
    PROi Q8R4T9.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8R4T9.
    Genevestigatori Q8R4T9.

    Family and domain databases

    Gene3Di 1.10.3430.10. 2 hits.
    InterProi IPR029020. Ammonium/urea_transptr.
    IPR004937. Urea_transporter.
    [Graphical view ]
    PANTHERi PTHR10464. PTHR10464. 1 hit.
    Pfami PF03253. UT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of a novel UT-A urea transporter isoform (UT-A5) in testis."
      Fenton R.A., Howorth A., Cooper G.J., Meccariello R., Morris I.D., Smith C.P.
      Am. J. Physiol. 279:C1425-C1431(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A3 AND A5), TISSUE SPECIFICITY (ISOFORM A5).
      Strain: MF1.
      Tissue: Kidney inner medulla and Testis.
    2. "Structure and characterization of the mouse UT-A gene (Slc14a2)."
      Fenton R.A., Cottingham C.A., Stewart G.S., Howorth A., Hewitt J.A., Smith C.P.
      Am. J. Physiol. 282:F630-F638(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A1 AND A2).
      Strain: MF1.
      Tissue: Kidney inner medulla.
    3. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A2), TISSUE SPECIFICITY.
      Strain: MF1.
      Tissue: Kidney inner medulla.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A1).
      Tissue: Brain.

    Entry informationi

    Entry nameiUT2_MOUSE
    AccessioniPrimary (citable) accession number: Q8R4T9
    Secondary accession number(s): B2RWS5
    , Q8K5D0, Q9ES04, Q9ES05
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 23, 2010
    Last sequence update: March 23, 2010
    Last modified: October 1, 2014
    This is version 69 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3