Urea transporter 2 - Mus musculus (Mouse)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Urea transporter 2

Alternative name(s):
Solute carrier family 14 member 2
Urea transporter, kidney

Gene names

Name:	Slc14a2
Synonyms:	Ut2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	930 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Specialized low-affinity vasopressin-regulated urea transporter. Mediates rapid transepithelial urea transport across the inner medullary collecting duct and plays a major role in the urinary concentrating mechanism. Vasopressin regulates urea transport by increasing isoform A1 accumulation in the plasma membrane and/or phosphorylation of isoform A1 By similarity.
Subunit structure	Isoform A1 interacts with SNAPIN which may be important for recruitment to the plasma membrane By similarity.
Subcellular location	Apical cell membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Highly expressed in kidney medulla. Also detected at the protein level in testes, heart, brain and liver. In the kidney, present in thin descending limbs of the loop of Henle and in the middle and terminal inner medullary collecting ducts. Isoform A5 is expressed in the peritubular myoid cells forming the outermost layer of the seminiferous tubules within the testes and is not detected in kidney. Isoform A5 mRNA levels are coordinated with the stage of testes development and increase 15 days postpartum, commensurate with the start of seminiferous tubule fluid movement. Ref.1 Ref.3
Sequence similarities	Belongs to the urea transporter family.
Caution	It is uncertain whether Met-1 or Met-10 is the initiator.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	apical plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from sequence or structural similarity Ref.1. Source: MGI
Molecular_function	urea transmembrane transporter activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform A1 (identifier: Q8R4T9-1) Also known as: UTA-1; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform A2 (identifier: Q8R4T9-2) Also known as: UTA-2; The sequence of this isoform differs from the canonical sequence as follows: 1-533: Missing.

Isoform A3 (identifier: Q8R4T9-3) Also known as: UTA-3; The sequence of this isoform differs from the canonical sequence as follows: 461-461: G → D 462-930: Missing.

Isoform A5 (identifier: Q8R4T9-4) Also known as: UTA-5; The sequence of this isoform differs from the canonical sequence as follows: 1-138: Missing. 461-461: G → D 462-930: Missing.
Note: Implicated in the urea movement across the blood-testis barrier. Does not translocate water. Expressed in testis, not expressed in kidney.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 930

930

Urea transporter 2

PRO_0000392531

Regions

Transmembrane

145 – 165

21

Helical; Potential

Transmembrane

185 – 205

21

Helical; Potential

Transmembrane

213 – 233

21

Helical; Potential

Transmembrane

242 – 262

21

Helical; Potential

Transmembrane

280 – 300

21

Helical; Potential

Transmembrane

311 – 331

21

Helical; Potential

Transmembrane

350 – 372

23

Helical; Potential

Transmembrane

401 – 421

21

Helical; Potential

Transmembrane

610 – 630

21

Helical; Potential

Transmembrane

648 – 668

21

Helical; Potential

Transmembrane

676 – 696

21

Helical; Potential

Transmembrane

705 – 725

21

Helical; Potential

Transmembrane

774 – 794

21

Helical; Potential

Transmembrane

813 – 833

21

Helical; Potential

Transmembrane

842 – 862

21

Helical; Potential

Transmembrane

864 – 884

21

Helical; Potential

Amino acid modifications

Modified residue

36

1

Phosphoserine By similarity

Modified residue

38

1

Phosphothreonine By similarity

Modified residue

63

1

Phosphoserine By similarity

Modified residue

64

1

Phosphoserine By similarity

Glycosylation

743

1

N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence

1 – 533

533

Missing in isoform A2.

VSP_038810

Alternative sequence

1 – 138

138

Missing in isoform A5.

VSP_038809

Alternative sequence

461

1

G → D in isoform A3 and isoform A5.

VSP_038811

Alternative sequence

462 – 930

469

Missing in isoform A3 and isoform A5.

VSP_038812

Experimental info

Sequence conflict

313

1

V → M in AAG32167. Ref.1

Sequence conflict

313

1

V → M in AAG32168. Ref.1

Sequence conflict

313

1

V → M in AAM00357. Ref.2

Sequence conflict

313

1

V → M in EDL09434. Ref.4

Sequence conflict

538

1

T → A in AAI50681. Ref.5

Sequence conflict

898

1

K → R in AAM00357. Ref.2

Sequence conflict

898

1

K → R in AAM21206. Ref.3

Sequences

Sequence		Length	Mass (Da)
Isoform A1 (UTA-1) [UniParc]. Last modified March 23, 2010. Version 2. Checksum: 298727BAFBE66D46 Show »	FASTA	930	102,047
10 20 30 40 50 60 MSDHHPLKEM SDSNSSPLLP EPLSSRYKLY ESELSSPTWP SSSQDTHPAL PLLEMPEEKD 70 80 90 100 110 120 LRSSDEDSHI VKIEKPNERN KRRESEVSRR ASAGRGGFSL FQAVSYLTGD MKECKNWLKD 130 140 150 160 170 180 KPLVLQFLDW VLRGAAQVMF VNNPISGLII FIGLLIQNPW WTIAGTLGTV ASTLAALALS 190 200 210 220 230 240 QDRSAIASGL HGYNGMLVGL LMAVFSEKLD YYWWLLFPVT FTSMACPIIS SALSTIFAKW 250 260 270 280 290 300 DLPVFTLPFN IALTLYLAAT GHYNLFFPTT LIKPASAAPN ITWTEIEMPL LLQTIPVGVG 310 320 330 340 350 360 QVYGCDNPWT GGVILVALFI SSPLICLHAA IGSIVGLLAA LTVATPFETI YLGLWSYNCV 370 380 390 400 410 420 LSCIAIGGMF YALTWQTHLL ALVCALFCAY MGAALSNTMA VVGVPSGTWA FCLSTLTFLL 430 440 450 460 470 480 LTSNNTGIYK LPLSKVTYPE ANRIYFLTVR RSEEEKSPNG GSGEQSHGSG QWKAEESSET 490 500 510 520 530 540 VLPRRRSVFH IEWSSIRRRS KVFGKGEHQE RQTKEPLPCP YRKPTVELFD LDTMEESTEI 550 560 570 580 590 600 KVEANTARTS WIQSSMVAGG KRVSKALSYI TGEMKECGEG LKDKSPVFQF LDWVLRGMSQ 610 620 630 640 650 660 VMFVNNPLSG ILIVLGLFVQ NPWWAISGCL GTVMSTLTAL ILSQDKSAIA AGLHGYNGVL 670 680 690 700 710 720 VGLLMAVFSD KGNYYWWLLL PVIVMSMTCP ILSSALSTVF SKWDLPVFTL PFNIAVTLYL 730 740 750 760 770 780 AATGHHNLFF PTTLLQPATT TPNITWSDIQ VSLLLRAIPV GIGQVYGCDN PWTGGIFLVA 790 800 810 820 830 840 LFISSPLICL HAAIGSTIGM LAALSIATPF DSIYFGLCGF NSTLACIAIG GMFYVITWQT 850 860 870 880 890 900 HLLAIACALF AAYLGAALAN MLSVFGLPPC TWPFCLSALT FLLLTSNNPA IYKLPLSKVT 910 920 930 YPEANRIYFL SQEKNRRAST ITKYQAYDVS « Hide
Isoform A2 (UTA-2) [UniParc]. Checksum: 4E24BE4C44956F4B Show »	FASTA	397	43,116
10 20 30 40 50 60 MEESTEIKVE ANTARTSWIQ SSMVAGGKRV SKALSYITGE MKECGEGLKD KSPVFQFLDW 70 80 90 100 110 120 VLRGMSQVMF VNNPLSGILI VLGLFVQNPW WAISGCLGTV MSTLTALILS QDKSAIAAGL 130 140 150 160 170 180 HGYNGVLVGL LMAVFSDKGN YYWWLLLPVI VMSMTCPILS SALSTVFSKW DLPVFTLPFN 190 200 210 220 230 240 IAVTLYLAAT GHHNLFFPTT LLQPATTTPN ITWSDIQVSL LLRAIPVGIG QVYGCDNPWT 250 260 270 280 290 300 GGIFLVALFI SSPLICLHAA IGSTIGMLAA LSIATPFDSI YFGLCGFNST LACIAIGGMF 310 320 330 340 350 360 YVITWQTHLL AIACALFAAY LGAALANMLS VFGLPPCTWP FCLSALTFLL LTSNNPAIYK 370 380 390 LPLSKVTYPE ANRIYFLSQE KNRRASTITK YQAYDVS « Hide
Isoform A3 (UTA-3) [UniParc]. Checksum: 18B0F3A3B6B18030 Show »	FASTA	461	50,615
10 20 30 40 50 60 MSDHHPLKEM SDSNSSPLLP EPLSSRYKLY ESELSSPTWP SSSQDTHPAL PLLEMPEEKD 70 80 90 100 110 120 LRSSDEDSHI VKIEKPNERN KRRESEVSRR ASAGRGGFSL FQAVSYLTGD MKECKNWLKD 130 140 150 160 170 180 KPLVLQFLDW VLRGAAQVMF VNNPISGLII FIGLLIQNPW WTIAGTLGTV ASTLAALALS 190 200 210 220 230 240 QDRSAIASGL HGYNGMLVGL LMAVFSEKLD YYWWLLFPVT FTSMACPIIS SALSTIFAKW 250 260 270 280 290 300 DLPVFTLPFN IALTLYLAAT GHYNLFFPTT LIKPASAAPN ITWTEIEMPL LLQTIPVGVG 310 320 330 340 350 360 QVYGCDNPWT GGVILVALFI SSPLICLHAA IGSIVGLLAA LTVATPFETI YLGLWSYNCV 370 380 390 400 410 420 LSCIAIGGMF YALTWQTHLL ALVCALFCAY MGAALSNTMA VVGVPSGTWA FCLSTLTFLL 430 440 450 460 LTSNNTGIYK LPLSKVTYPE ANRIYFLTVR RSEEEKSPNG D « Hide
Isoform A5 (UTA-5) [UniParc]. Checksum: 6B9774A12E42D609 Show »	FASTA	323	34,921
10 20 30 40 50 60 MFVNNPISGL IIFIGLLIQN PWWTIAGTLG TVASTLAALA LSQDRSAIAS GLHGYNGMLV 70 80 90 100 110 120 GLLMAVFSEK LDYYWWLLFP VTFTSMACPI ISSALSTIFA KWDLPVFTLP FNIALTLYLA 130 140 150 160 170 180 ATGHYNLFFP TTLIKPASAA PNITWTEIEM PLLLQTIPVG VGQVYGCDNP WTGGVILVAL 190 200 210 220 230 240 FISSPLICLH AAIGSIVGLL AALTVATPFE TIYLGLWSYN CVLSCIAIGG MFYALTWQTH 250 260 270 280 290 300 LLALVCALFC AYMGAALSNT MAVVGVPSGT WAFCLSTLTF LLLTSNNTGI YKLPLSKVTY 310 320 PEANRIYFLT VRRSEEEKSP NGD « Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of a novel UT-A urea transporter isoform (UT-A5) in testis." Fenton R.A., Howorth A., Cooper G.J., Meccariello R., Morris I.D., Smith C.P. Am. J. Physiol. 279:C1425-C1431(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A3 AND A5), TISSUE SPECIFICITY (ISOFORM A5). Strain: MF1. Tissue: Kidney inner medulla and Testis.
[2]	"Structure and characterization of the mouse UT-A gene (Slc14a2)." Fenton R.A., Cottingham C.A., Stewart G.S., Howorth A., Hewitt J.A., Smith C.P. Am. J. Physiol. 282:F630-F638(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A1 AND A2). Strain: MF1. Tissue: Kidney inner medulla.
[3]	"Characterization of mouse urea transporters UT-A1 and UT-A2." Fenton R.A., Stewart G.S., Carpenter B., Howorth A., Potter E.A., Cooper G.J., Smith C.P. Am. J. Physiol. 283:F817-F825(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A2), TISSUE SPECIFICITY. Strain: MF1. Tissue: Kidney inner medulla.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A1). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF258601 mRNA. Translation: AAG32167.1. AF258602 mRNA. Translation: AAG32168.1. AF366052 mRNA. Translation: AAM00357.1. AF367359 mRNA. Translation: AAM21206.1. CH466528 Genomic DNA. Translation: EDL09434.1. BC150680 mRNA. Translation: AAI50681.1.
IPI	IPI00322187. IPI00410960. IPI00877227. IPI00955727.
RefSeq	NP_001103744.1. NM_001110274.1. NP_109608.1. NM_030683.3.
UniGene	Mm.44158.
3D structure databases
ProteinModelPortal	Q8R4T9.
SMR	Q8R4T9. Positions 106-450, 569-910.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000025434.
PTM databases
PhosphoSite	Q8R4T9.
Proteomic databases
PaxDb	Q8R4T9.
PRIDE	Q8R4T9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000163367; ENSMUSP00000126416; ENSMUSG00000024552.
GeneID	27411.
KEGG	mmu:27411.
UCSC	uc008fsf.2. mouse. uc008fsh.2. mouse.
Organism-specific databases
CTD	8170.
MGI	MGI:1351653. Slc14a2.
Phylogenomic databases
eggNOG	COG4413.
GeneTree	ENSGT00390000018729.
HOGENOM	HOG000168437.
HOVERGEN	HBG073255.
InParanoid	Q8R4T9.
KO	K08716.
OrthoDB	EOG48D0VG.
Gene expression databases
ArrayExpress	Q8R4T9.
Bgee	Q8R4T9.
Genevestigator	Q8R4T9.
Family and domain databases
InterPro	IPR004937. Urea_transporter. [Graphical view]
PANTHER	PTHR10464. PTHR10464. 1 hit.
Pfam	PF03253. UT. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	305432.
SOURCE	Search...

Entry information

Entry name

UT2_MOUSE

Accession

Primary (citable) accession number: Q8R4T9
Secondary accession number(s): B2RWS5

Q9ES05

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 23, 2010
Last sequence update:	March 23, 2010
Last modified:	May 1, 2013
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents