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Q8R4T9 (UT2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Urea transporter 2
Alternative name(s):
Solute carrier family 14 member 2
Urea transporter, kidney
Gene names
Name:Slc14a2
Synonyms:Ut2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length930 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Specialized low-affinity vasopressin-regulated urea transporter. Mediates rapid transepithelial urea transport across the inner medullary collecting duct and plays a major role in the urinary concentrating mechanism. Vasopressin regulates urea transport by increasing isoform A1 accumulation in the plasma membrane and/or phosphorylation of isoform A1 By similarity.

Subunit structure

Isoform A1 interacts with SNAPIN which may be important for recruitment to the plasma membrane By similarity.

Subcellular location

Apical cell membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Highly expressed in kidney medulla. Also detected at the protein level in testes, heart, brain and liver. In the kidney, present in thin descending limbs of the loop of Henle and in the middle and terminal inner medullary collecting ducts. Isoform A5 is expressed in the peritubular myoid cells forming the outermost layer of the seminiferous tubules within the testes and is not detected in kidney. Isoform A5 mRNA levels are coordinated with the stage of testes development and increase 15 days postpartum, commensurate with the start of seminiferous tubule fluid movement. Ref.1 Ref.3

Sequence similarities

Belongs to the urea transporter family.

Caution

It is uncertain whether Met-1 or Met-10 is the initiator.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform A1 (identifier: Q8R4T9-1)

Also known as: UTA-1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform A2 (identifier: Q8R4T9-2)

Also known as: UTA-2;

The sequence of this isoform differs from the canonical sequence as follows:
     1-533: Missing.
Isoform A3 (identifier: Q8R4T9-3)

Also known as: UTA-3;

The sequence of this isoform differs from the canonical sequence as follows:
     461-461: G → D
     462-930: Missing.
Isoform A5 (identifier: Q8R4T9-4)

Also known as: UTA-5;

The sequence of this isoform differs from the canonical sequence as follows:
     1-138: Missing.
     461-461: G → D
     462-930: Missing.
Note: Implicated in the urea movement across the blood-testis barrier. Does not translocate water. Expressed in testis, not expressed in kidney.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 930930Urea transporter 2
PRO_0000392531

Regions

Transmembrane145 – 16521Helical; Potential
Transmembrane185 – 20521Helical; Potential
Transmembrane213 – 23321Helical; Potential
Transmembrane242 – 26221Helical; Potential
Transmembrane280 – 30021Helical; Potential
Transmembrane311 – 33121Helical; Potential
Transmembrane350 – 37223Helical; Potential
Transmembrane401 – 42121Helical; Potential
Transmembrane610 – 63021Helical; Potential
Transmembrane648 – 66821Helical; Potential
Transmembrane676 – 69621Helical; Potential
Transmembrane705 – 72521Helical; Potential
Transmembrane774 – 79421Helical; Potential
Transmembrane813 – 83321Helical; Potential
Transmembrane842 – 86221Helical; Potential
Transmembrane864 – 88421Helical; Potential

Amino acid modifications

Modified residue4871Phosphoserine By similarity
Glycosylation7431N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 533533Missing in isoform A2.
VSP_038810
Alternative sequence1 – 138138Missing in isoform A5.
VSP_038809
Alternative sequence4611G → D in isoform A3 and isoform A5.
VSP_038811
Alternative sequence462 – 930469Missing in isoform A3 and isoform A5.
VSP_038812

Experimental info

Sequence conflict3131V → M in AAG32167. Ref.1
Sequence conflict3131V → M in AAG32168. Ref.1
Sequence conflict3131V → M in AAM00357. Ref.2
Sequence conflict3131V → M in EDL09434. Ref.4
Sequence conflict5381T → A in AAI50681. Ref.5
Sequence conflict8981K → R in AAM00357. Ref.2
Sequence conflict8981K → R in AAM21206. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform A1 (UTA-1) [UniParc].

Last modified March 23, 2010. Version 2.
Checksum: 298727BAFBE66D46

FASTA930102,047
        10         20         30         40         50         60 
MSDHHPLKEM SDSNSSPLLP EPLSSRYKLY ESELSSPTWP SSSQDTHPAL PLLEMPEEKD 

        70         80         90        100        110        120 
LRSSDEDSHI VKIEKPNERN KRRESEVSRR ASAGRGGFSL FQAVSYLTGD MKECKNWLKD 

       130        140        150        160        170        180 
KPLVLQFLDW VLRGAAQVMF VNNPISGLII FIGLLIQNPW WTIAGTLGTV ASTLAALALS 

       190        200        210        220        230        240 
QDRSAIASGL HGYNGMLVGL LMAVFSEKLD YYWWLLFPVT FTSMACPIIS SALSTIFAKW 

       250        260        270        280        290        300 
DLPVFTLPFN IALTLYLAAT GHYNLFFPTT LIKPASAAPN ITWTEIEMPL LLQTIPVGVG 

       310        320        330        340        350        360 
QVYGCDNPWT GGVILVALFI SSPLICLHAA IGSIVGLLAA LTVATPFETI YLGLWSYNCV 

       370        380        390        400        410        420 
LSCIAIGGMF YALTWQTHLL ALVCALFCAY MGAALSNTMA VVGVPSGTWA FCLSTLTFLL 

       430        440        450        460        470        480 
LTSNNTGIYK LPLSKVTYPE ANRIYFLTVR RSEEEKSPNG GSGEQSHGSG QWKAEESSET 

       490        500        510        520        530        540 
VLPRRRSVFH IEWSSIRRRS KVFGKGEHQE RQTKEPLPCP YRKPTVELFD LDTMEESTEI 

       550        560        570        580        590        600 
KVEANTARTS WIQSSMVAGG KRVSKALSYI TGEMKECGEG LKDKSPVFQF LDWVLRGMSQ 

       610        620        630        640        650        660 
VMFVNNPLSG ILIVLGLFVQ NPWWAISGCL GTVMSTLTAL ILSQDKSAIA AGLHGYNGVL 

       670        680        690        700        710        720 
VGLLMAVFSD KGNYYWWLLL PVIVMSMTCP ILSSALSTVF SKWDLPVFTL PFNIAVTLYL 

       730        740        750        760        770        780 
AATGHHNLFF PTTLLQPATT TPNITWSDIQ VSLLLRAIPV GIGQVYGCDN PWTGGIFLVA 

       790        800        810        820        830        840 
LFISSPLICL HAAIGSTIGM LAALSIATPF DSIYFGLCGF NSTLACIAIG GMFYVITWQT 

       850        860        870        880        890        900 
HLLAIACALF AAYLGAALAN MLSVFGLPPC TWPFCLSALT FLLLTSNNPA IYKLPLSKVT 

       910        920        930 
YPEANRIYFL SQEKNRRAST ITKYQAYDVS 

« Hide

Isoform A2 (UTA-2) [UniParc].

Checksum: 4E24BE4C44956F4B
Show »

FASTA39743,116
Isoform A3 (UTA-3) [UniParc].

Checksum: 18B0F3A3B6B18030
Show »

FASTA46150,615
Isoform A5 (UTA-5) [UniParc].

Checksum: 6B9774A12E42D609
Show »

FASTA32334,921

References

« Hide 'large scale' references
[1]"Molecular characterization of a novel UT-A urea transporter isoform (UT-A5) in testis."
Fenton R.A., Howorth A., Cooper G.J., Meccariello R., Morris I.D., Smith C.P.
Am. J. Physiol. 279:C1425-C1431(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A3 AND A5), TISSUE SPECIFICITY (ISOFORM A5).
Strain: MF1.
Tissue: Kidney inner medulla and Testis.
[2]"Structure and characterization of the mouse UT-A gene (Slc14a2)."
Fenton R.A., Cottingham C.A., Stewart G.S., Howorth A., Hewitt J.A., Smith C.P.
Am. J. Physiol. 282:F630-F638(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A1 AND A2).
Strain: MF1.
Tissue: Kidney inner medulla.
[3]"Characterization of mouse urea transporters UT-A1 and UT-A2."
Fenton R.A., Stewart G.S., Carpenter B., Howorth A., Potter E.A., Cooper G.J., Smith C.P.
Am. J. Physiol. 283:F817-F825(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A2), TISSUE SPECIFICITY.
Strain: MF1.
Tissue: Kidney inner medulla.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A1).
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF258601 mRNA. Translation: AAG32167.1.
AF258602 mRNA. Translation: AAG32168.1.
AF366052 mRNA. Translation: AAM00357.1.
AF367359 mRNA. Translation: AAM21206.1.
CH466528 Genomic DNA. Translation: EDL09434.1.
BC150680 mRNA. Translation: AAI50681.1.
RefSeqNP_001103744.1. NM_001110274.1.
NP_109608.1. NM_030683.3.
UniGeneMm.44158.

3D structure databases

ProteinModelPortalQ8R4T9.
SMRQ8R4T9. Positions 106-450, 569-910.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000025434.

PTM databases

PhosphoSiteQ8R4T9.

Proteomic databases

PaxDbQ8R4T9.
PRIDEQ8R4T9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000163367; ENSMUSP00000126416; ENSMUSG00000024552.
GeneID27411.
KEGGmmu:27411.
UCSCuc008fsh.2. mouse. [Q8R4T9-3]
uc012bfh.1. mouse. [Q8R4T9-4]

Organism-specific databases

CTD8170.
MGIMGI:1351653. Slc14a2.

Phylogenomic databases

eggNOGCOG4413.
GeneTreeENSGT00390000018729.
HOGENOMHOG000168437.
HOVERGENHBG073255.
InParanoidQ8R4T9.
KOK08716.
PhylomeDBQ8R4T9.

Gene expression databases

BgeeQ8R4T9.
GenevestigatorQ8R4T9.

Family and domain databases

InterProIPR004937. Urea_transporter.
[Graphical view]
PANTHERPTHR10464. PTHR10464. 1 hit.
PfamPF03253. UT. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio305432.
PROQ8R4T9.
SOURCESearch...

Entry information

Entry nameUT2_MOUSE
AccessionPrimary (citable) accession number: Q8R4T9
Secondary accession number(s): B2RWS5 expand/collapse secondary AC list , Q8K5D0, Q9ES04, Q9ES05
Entry history
Integrated into UniProtKB/Swiss-Prot: March 23, 2010
Last sequence update: March 23, 2010
Last modified: April 16, 2014
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot