ID   GRASP_RAT               Reviewed;         394 AA.
AC   Q8R4T5;
DT   27-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   24-JAN-2024, entry version 137.
DE   RecName: Full=General receptor for phosphoinositides 1-associated scaffold protein;
DE            Short=GRP1-associated scaffold protein;
DE   AltName: Full=95 kDa postsynaptic density protein discs-large ZO-1 domain-containing protein;
DE   AltName: Full=PSD-95 PDZ domain-containing protein;
DE   AltName: Full=Tamalin {ECO:0000303|PubMed:11850456};
GN   Name=Tamalin {ECO:0000303|PubMed:11850456}; Synonyms=Grasp;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH GRM1;
RP   GRM2; GRM3; GRM5 AND CYTH2, SUBCELLULAR LOCATION, AND FUNCTION.
RC   TISSUE=Brain;
RX   PubMed=11850456; DOI=10.1523/jneurosci.22-04-01280.2002;
RA   Kitano J., Kimura K., Yamazaki Y., Soda T., Shigemoto R., Nakajima Y.,
RA   Nakanishi S.;
RT   "Tamalin, a PDZ domain-containing protein, links a protein complex
RT   formation of group 1 metabotropic glutamate receptors and the guanine
RT   nucleotide exchange factor cytohesins.";
RL   J. Neurosci. 22:1280-1289(2002).
RN   [2]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-93 AND SER-386, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22673903; DOI=10.1038/ncomms1871;
RA   Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA   Olsen J.V.;
RT   "Quantitative maps of protein phosphorylation sites across 14 different rat
RT   organs and tissues.";
RL   Nat. Commun. 3:876-876(2012).
CC   -!- FUNCTION: Plays a role in intracellular trafficking and contributes to
CC       the macromolecular organization of group 1 metabotropic glutamate
CC       receptors (mGluRs) at synapses. {ECO:0000269|PubMed:11850456}.
CC   -!- SUBUNIT: Heteromer. Composed of TAMALIN, CYTH2 and at least one GRM1.
CC       Also interacts with CYTH3, GRM2, GRM3 and GRM5.
CC       {ECO:0000250|UniProtKB:Q9JJA9, ECO:0000269|PubMed:11850456}.
CC   -!- INTERACTION:
CC       Q8R4T5; O35431: Apba2; NbExp=4; IntAct=EBI-7361884, EBI-2028211;
CC       Q8R4T5; P31016: Dlg4; NbExp=3; IntAct=EBI-7361884, EBI-375655;
CC       Q8R4T5; P97838: Dlgap3; NbExp=3; IntAct=EBI-7361884, EBI-375673;
CC       Q8R4T5; P31424: Grm5; NbExp=5; IntAct=EBI-7361884, EBI-2902734;
CC       Q8R4T5; O88382: Magi2; NbExp=5; IntAct=EBI-7361884, EBI-696179;
CC       Q8R4T5; Q8R4T5: Tamalin; NbExp=3; IntAct=EBI-7361884, EBI-7361884;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region
CC       {ECO:0000269|PubMed:11850456}. Cell membrane
CC       {ECO:0000269|PubMed:11850456}; Peripheral membrane protein
CC       {ECO:0000269|PubMed:11850456}; Cytoplasmic side
CC       {ECO:0000269|PubMed:11850456}. Postsynaptic cell membrane
CC       {ECO:0000269|PubMed:11850456}.
CC   -!- TISSUE SPECIFICITY: Expressed in brain. {ECO:0000269|PubMed:11850456}.
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DR   EMBL; AF374272; AAL87038.1; -; mRNA.
DR   RefSeq; NP_620249.1; NM_138894.1.
DR   PDB; 2EGK; X-ray; 2.85 A; A/B/C/D=96-189.
DR   PDB; 2EGN; X-ray; 2.40 A; A=96-189.
DR   PDB; 2EGO; X-ray; 1.80 A; A/B=96-189.
DR   PDBsum; 2EGK; -.
DR   PDBsum; 2EGN; -.
DR   PDBsum; 2EGO; -.
DR   AlphaFoldDB; Q8R4T5; -.
DR   SMR; Q8R4T5; -.
DR   BioGRID; 251380; 19.
DR   CORUM; Q8R4T5; -.
DR   ELM; Q8R4T5; -.
DR   IntAct; Q8R4T5; 16.
DR   MINT; Q8R4T5; -.
DR   STRING; 10116.ENSRNOP00000009979; -.
DR   iPTMnet; Q8R4T5; -.
DR   PhosphoSitePlus; Q8R4T5; -.
DR   PaxDb; 10116-ENSRNOP00000009979; -.
DR   Ensembl; ENSRNOT00000009979.4; ENSRNOP00000009979.1; ENSRNOG00000007346.4.
DR   Ensembl; ENSRNOT00065039388; ENSRNOP00065032016; ENSRNOG00065023049.
DR   GeneID; 192254; -.
DR   KEGG; rno:192254; -.
DR   UCSC; RGD:70554; rat.
DR   AGR; RGD:70554; -.
DR   CTD; 160622; -.
DR   RGD; 70554; Tamalin.
DR   eggNOG; KOG3528; Eukaryota.
DR   GeneTree; ENSGT00530000063734; -.
DR   HOGENOM; CLU_058640_0_0_1; -.
DR   InParanoid; Q8R4T5; -.
DR   OMA; KVHNDSP; -.
DR   OrthoDB; 5356422at2759; -.
DR   PhylomeDB; Q8R4T5; -.
DR   TreeFam; TF316315; -.
DR   EvolutionaryTrace; Q8R4T5; -.
DR   PRO; PR:Q8R4T5; -.
DR   Proteomes; UP000002494; Chromosome 7.
DR   Bgee; ENSRNOG00000007346; Expressed in frontal cortex and 18 other cell types or tissues.
DR   ExpressionAtlas; Q8R4T5; baseline and differential.
DR   GO; GO:0098978; C:glutamatergic synapse; IDA:SynGO.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; ISO:RGD.
DR   GO; GO:0014069; C:postsynaptic density; IDA:SynGO.
DR   GO; GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0098685; C:Schaffer collateral - CA1 synapse; ISO:RGD.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0030165; F:PDZ domain binding; IDA:RGD.
DR   GO; GO:0031267; F:small GTPase binding; IDA:RGD.
DR   GO; GO:0099152; P:regulation of neurotransmitter receptor transport, endosome to postsynaptic membrane; ISO:RGD.
DR   GO; GO:0007165; P:signal transduction; ISO:RGD.
DR   CDD; cd00992; PDZ_signaling; 1.
DR   Gene3D; 2.30.42.10; -; 1.
DR   InterPro; IPR001478; PDZ.
DR   InterPro; IPR036034; PDZ_sf.
DR   PANTHER; PTHR15963; GENERAL RECEPTOR FOR PHOSPHOINOSITIDES 1-ASSOCIATED SCAFFOLD PROTEIN-RELATED; 1.
DR   PANTHER; PTHR15963:SF3; PROTEIN TAMALIN; 1.
DR   Pfam; PF00595; PDZ; 1.
DR   SMART; SM00228; PDZ; 1.
DR   SUPFAM; SSF50156; PDZ domain-like; 1.
DR   PROSITE; PS50106; PDZ; 1.
DR   Genevisible; Q8R4T5; RN.
PE   1: Evidence at protein level;
KW   3D-structure; Cell membrane; Cytoplasm; Membrane; Methylation;
KW   Phosphoprotein; Postsynaptic cell membrane; Reference proteome; Synapse.
FT   CHAIN           1..394
FT                   /note="General receptor for phosphoinositides 1-associated
FT                   scaffold protein"
FT                   /id="PRO_0000087586"
FT   DOMAIN          100..189
FT                   /note="PDZ"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT   REGION          1..51
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          180..257
FT                   /note="Interaction with PSCD3"
FT                   /evidence="ECO:0000250"
FT   REGION          293..318
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        294..309
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         76
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9JJA9"
FT   MOD_RES         93
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
FT   MOD_RES         236
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0000250|UniProtKB:Q7Z6J2"
FT   MOD_RES         269
FT                   /note="Omega-N-methylarginine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9JJA9"
FT   MOD_RES         386
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
FT   STRAND          98..104
FT                   /evidence="ECO:0007829|PDB:2EGO"
FT   STRAND          107..109
FT                   /evidence="ECO:0007829|PDB:2EGK"
FT   STRAND          112..119
FT                   /evidence="ECO:0007829|PDB:2EGO"
FT   STRAND          130..137
FT                   /evidence="ECO:0007829|PDB:2EGO"
FT   HELIX           142..145
FT                   /evidence="ECO:0007829|PDB:2EGO"
FT   STRAND          153..157
FT                   /evidence="ECO:0007829|PDB:2EGO"
FT   HELIX           167..176
FT                   /evidence="ECO:0007829|PDB:2EGO"
FT   TURN            177..179
FT                   /evidence="ECO:0007829|PDB:2EGO"
FT   STRAND          180..186
FT                   /evidence="ECO:0007829|PDB:2EGO"
SQ   SEQUENCE   394 AA;  42321 MW;  520D0AD23CB2B70D CRC64;
     MTLRRLRKLQ QKEEATAAPD LAGRAPDSEA ARAAPTPSGP PAAAAPPGAP GDELYAALED
     YHPAELYRAL AVSGGTLPRR KGSGFRWKNF TQSPEQQRKV LTLEKGDNQT FGFEIQTYGL
     HHREEQRVEM VTFVCRVHES SPAQLAGLTP GDTIASVNGL NVEGIRHREI VDIIKASGNV
     LRLETLYGTS IRKAELEARL QYLKQTLYEK WGEYRSLMVQ EQRLVHGLVV KDPSIYDTLE
     SVRSCLYGAG LLPGSLPFGP LLAAPGGARG GSRRAKGDTD DAVYHTCFFG GAEPQALPPP
     PPPARAPGPG SAETPASVLC PAPRATLSRS ASVRCAGPGG GGGGGAPGAL WTEAREQALC
     GAGLRKTKYR SFRRRLLKFI PGLNRSLEEE ESQL
//