General receptor for phosphoinositides 1-associated scaffold protein

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Q8R4T5 (GRASP_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 73. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
General receptor for phosphoinositides 1-associated scaffold protein
Short name=GRP1-associated scaffold protein

Alternative name(s):
95 kDa postsynaptic density protein discs-large ZO-1 domain-containing protein
PSD-95 PDZ domain-containing protein
Tamalin

Gene names

Name:

Grasp

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in intracellular trafficking and contributes to the macromolecular organization of group 1 metabotropic glutamate receptors (mGluRs) at synapses. Ref.1
Subunit structure	Interacts with PSCD3 By similarity. Heteromer. Composed of GRASP, PSCD2 and at least one GRM1. Also interacts with GRM2, GRM3 and GRM5. Ref.1
Subcellular location	Cytoplasm › perinuclear region. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction › synapse › postsynaptic cell membrane. Note: Localized at postsynaptic membranes. Ref.1
Tissue specificity	Expressed in brain. Ref.1
Sequence similarities	Contains 1 PDZ (DHR) domain.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Cytoplasm Membrane Postsynaptic cell membrane Synapse
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein localization Inferred from direct assay Ref.1. Source: RGD signal transduction Inferred from electronic annotation. Source: Compara
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay Ref.1. Source: RGD postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ADP-ribosylation factor binding Inferred from direct assay Ref.1. Source: RGD PDZ domain binding Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 394

394

General receptor for phosphoinositides 1-associated scaffold protein

PRO_0000087586

Regions

Domain

100 – 189

PDZ

Region

180 – 257

Interaction with PSCD3 By similarity

Compositional bias

294 – 323

Pro-rich

Amino acid modifications

Modified residue

Phosphoserine By similarity

Modified residue

236

Phosphotyrosine By similarity

Secondary structure

394

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q8R4T5 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 520D0AD23CB2B70D
Show »

FASTA

394

42,321

        10         20         30         40         50         60 
MTLRRLRKLQ QKEEATAAPD LAGRAPDSEA ARAAPTPSGP PAAAAPPGAP GDELYAALED 

        70         80         90        100        110        120 
YHPAELYRAL AVSGGTLPRR KGSGFRWKNF TQSPEQQRKV LTLEKGDNQT FGFEIQTYGL 

       130        140        150        160        170        180 
HHREEQRVEM VTFVCRVHES SPAQLAGLTP GDTIASVNGL NVEGIRHREI VDIIKASGNV 

       190        200        210        220        230        240 
LRLETLYGTS IRKAELEARL QYLKQTLYEK WGEYRSLMVQ EQRLVHGLVV KDPSIYDTLE 

       250        260        270        280        290        300 
SVRSCLYGAG LLPGSLPFGP LLAAPGGARG GSRRAKGDTD DAVYHTCFFG GAEPQALPPP 

       310        320        330        340        350        360 
PPPARAPGPG SAETPASVLC PAPRATLSRS ASVRCAGPGG GGGGGAPGAL WTEAREQALC 

       370        380        390 
GAGLRKTKYR SFRRRLLKFI PGLNRSLEEE ESQL

« Hide

References

[1]

"Tamalin, a PDZ domain-containing protein, links a protein complex formation of group 1 metabotropic glutamate receptors and the guanine nucleotide exchange factor cytohesins."
Kitano J., Kimura K., Yamazaki Y., Soda T., Shigemoto R., Nakajima Y., Nakanishi S.
J. Neurosci. 22:1280-1289(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH GRM1; GRM2; GRM3; GRM5 AND PSCD2, SUBCELLULAR LOCATION, FUNCTION.
Tissue: Brain.

Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF374272 mRNA. Translation: AAL87038.1.

IPI

IPI00201161.

RefSeq

NP_620249.1. NM_138894.1.

UniGene

Rn.229849.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2EGK	X-ray	2.85	A/B/C/D	96-394	[»]
2EGN	X-ray	2.40	A	96-189	[»]
2EGO	X-ray	1.80	A/B	96-189	[»]

ProteinModelPortal

Q8R4T5.

SMR

Q8R4T5. Positions 97-191.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-1790327.

STRING

10116.ENSRNOP00000009979.

PTM databases

PhosphoSite

Q8R4T5.

Proteomic databases

PaxDb

Q8R4T5.

PRIDE

Q8R4T5.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSRNOT00000009979; ENSRNOP00000009979; ENSRNOG00000007346.

GeneID

192254.

KEGG

rno:192254.

UCSC

RGD:70554. rat.

Organism-specific databases

CTD

160622.

RGD

70554. Grasp.

Phylogenomic databases

eggNOG

NOG244814.

GeneTree

ENSGT00530000063734.

HOGENOM

HOG000231871.

HOVERGEN

HBG053061.

InParanoid

Q8R4T5.

OMA

EGFRHKE.

OrthoDB

EOG47D9GP.

Gene expression databases

Genevestigator

Q8R4T5.

GermOnline

ENSRNOG00000007346. Rattus norvegicus.

Family and domain databases

InterPro

IPR001478. PDZ.
[Graphical view]

Pfam

PF00595. PDZ. 1 hit.
[Graphical view]

SMART

SM00228. PDZ. 1 hit.
[Graphical view]

SUPFAM

SSF50156. PDZ. 1 hit.

PROSITE

PS50106. PDZ. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q8R4T5.

NextBio

622908.

Entry information

Entry name

GRASP_RAT

Accession

Primary (citable) accession number: Q8R4T5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	June 1, 2002
Last modified:	April 3, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents