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Protein

General receptor for phosphoinositides 1-associated scaffold protein

Gene

Grasp

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in intracellular trafficking and contributes to the macromolecular organization of group 1 metabotropic glutamate receptors (mGluRs) at synapses.1 Publication

GO - Molecular functioni

  • ADP-ribosylation factor binding Source: RGD
  • identical protein binding Source: IntAct
  • PDZ domain binding Source: RGD

GO - Biological processi

  • protein localization Source: RGD
  • signal transduction Source: Ensembl
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
General receptor for phosphoinositides 1-associated scaffold protein
Short name:
GRP1-associated scaffold protein
Alternative name(s):
95 kDa postsynaptic density protein discs-large ZO-1 domain-containing protein
PSD-95 PDZ domain-containing protein
Tamalin
Gene namesi
Name:Grasp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi70554. Grasp.

Subcellular locationi

  • Cytoplasmperinuclear region 1 Publication
  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
  • Cell junctionsynapsepostsynaptic cell membrane 1 Publication

  • Note: Localized at postsynaptic membranes.

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
  • plasma membrane Source: RGD
  • postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 394394General receptor for phosphoinositides 1-associated scaffold proteinPRO_0000087586Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei236 – 2361PhosphotyrosineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8R4T5.
PRIDEiQ8R4T5.

PTM databases

PhosphoSiteiQ8R4T5.

Expressioni

Tissue specificityi

Expressed in brain.1 Publication

Gene expression databases

GenevisibleiQ8R4T5. RN.

Interactioni

Subunit structurei

Interacts with PSCD3 (By similarity). Heteromer. Composed of GRASP, PSCD2 and at least one GRM1. Also interacts with GRM2, GRM3 and GRM5.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-7361884,EBI-7361884
Apba2O354314EBI-7361884,EBI-2028211
Dlg4P310163EBI-7361884,EBI-375655
Dlgap3P978383EBI-7361884,EBI-375673
Grm5P314245EBI-7361884,EBI-2902734
Magi2O883825EBI-7361884,EBI-696179

Protein-protein interaction databases

BioGridi251380. 14 interactions.
IntActiQ8R4T5. 16 interactions.
MINTiMINT-1790327.
STRINGi10116.ENSRNOP00000009979.

Structurei

Secondary structure

1
394
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi98 – 1047Combined sources
Beta strandi107 – 1093Combined sources
Beta strandi112 – 1198Combined sources
Beta strandi130 – 1378Combined sources
Helixi142 – 1454Combined sources
Beta strandi153 – 1575Combined sources
Helixi167 – 17610Combined sources
Turni177 – 1793Combined sources
Beta strandi180 – 1867Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EGKX-ray2.85A/B/C/D96-394[»]
2EGNX-ray2.40A96-189[»]
2EGOX-ray1.80A/B96-189[»]
ProteinModelPortaliQ8R4T5.
SMRiQ8R4T5. Positions 97-191.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8R4T5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini100 – 18990PDZPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni180 – 25778Interaction with PSCD3By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi294 – 32330Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG244814.
GeneTreeiENSGT00530000063734.
HOGENOMiHOG000231871.
HOVERGENiHBG053061.
InParanoidiQ8R4T5.
OMAiEGFRHKE.
OrthoDBiEOG72NRQP.
PhylomeDBiQ8R4T5.
TreeFamiTF316315.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR001478. PDZ.
[Graphical view]
PfamiPF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS50106. PDZ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R4T5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLRRLRKLQ QKEEATAAPD LAGRAPDSEA ARAAPTPSGP PAAAAPPGAP
60 70 80 90 100
GDELYAALED YHPAELYRAL AVSGGTLPRR KGSGFRWKNF TQSPEQQRKV
110 120 130 140 150
LTLEKGDNQT FGFEIQTYGL HHREEQRVEM VTFVCRVHES SPAQLAGLTP
160 170 180 190 200
GDTIASVNGL NVEGIRHREI VDIIKASGNV LRLETLYGTS IRKAELEARL
210 220 230 240 250
QYLKQTLYEK WGEYRSLMVQ EQRLVHGLVV KDPSIYDTLE SVRSCLYGAG
260 270 280 290 300
LLPGSLPFGP LLAAPGGARG GSRRAKGDTD DAVYHTCFFG GAEPQALPPP
310 320 330 340 350
PPPARAPGPG SAETPASVLC PAPRATLSRS ASVRCAGPGG GGGGGAPGAL
360 370 380 390
WTEAREQALC GAGLRKTKYR SFRRRLLKFI PGLNRSLEEE ESQL
Length:394
Mass (Da):42,321
Last modified:June 1, 2002 - v1
Checksum:i520D0AD23CB2B70D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF374272 mRNA. Translation: AAL87038.1.
RefSeqiNP_620249.1. NM_138894.1.
UniGeneiRn.229849.

Genome annotation databases

EnsembliENSRNOT00000009979; ENSRNOP00000009979; ENSRNOG00000007346.
GeneIDi192254.
KEGGirno:192254.
UCSCiRGD:70554. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF374272 mRNA. Translation: AAL87038.1.
RefSeqiNP_620249.1. NM_138894.1.
UniGeneiRn.229849.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EGKX-ray2.85A/B/C/D96-394[»]
2EGNX-ray2.40A96-189[»]
2EGOX-ray1.80A/B96-189[»]
ProteinModelPortaliQ8R4T5.
SMRiQ8R4T5. Positions 97-191.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251380. 14 interactions.
IntActiQ8R4T5. 16 interactions.
MINTiMINT-1790327.
STRINGi10116.ENSRNOP00000009979.

PTM databases

PhosphoSiteiQ8R4T5.

Proteomic databases

PaxDbiQ8R4T5.
PRIDEiQ8R4T5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009979; ENSRNOP00000009979; ENSRNOG00000007346.
GeneIDi192254.
KEGGirno:192254.
UCSCiRGD:70554. rat.

Organism-specific databases

CTDi160622.
RGDi70554. Grasp.

Phylogenomic databases

eggNOGiNOG244814.
GeneTreeiENSGT00530000063734.
HOGENOMiHOG000231871.
HOVERGENiHBG053061.
InParanoidiQ8R4T5.
OMAiEGFRHKE.
OrthoDBiEOG72NRQP.
PhylomeDBiQ8R4T5.
TreeFamiTF316315.

Miscellaneous databases

EvolutionaryTraceiQ8R4T5.
NextBioi622908.
PROiQ8R4T5.

Gene expression databases

GenevisibleiQ8R4T5. RN.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR001478. PDZ.
[Graphical view]
PfamiPF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Tamalin, a PDZ domain-containing protein, links a protein complex formation of group 1 metabotropic glutamate receptors and the guanine nucleotide exchange factor cytohesins."
    Kitano J., Kimura K., Yamazaki Y., Soda T., Shigemoto R., Nakajima Y., Nakanishi S.
    J. Neurosci. 22:1280-1289(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH GRM1; GRM2; GRM3; GRM5 AND PSCD2, SUBCELLULAR LOCATION, FUNCTION.
    Tissue: Brain.

Entry informationi

Entry nameiGRASP_RAT
AccessioniPrimary (citable) accession number: Q8R4T5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: June 1, 2002
Last modified: June 24, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.