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Protein

General receptor for phosphoinositides 1-associated scaffold protein

Gene

Grasp

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in intracellular trafficking and contributes to the macromolecular organization of group 1 metabotropic glutamate receptors (mGluRs) at synapses.1 Publication

GO - Molecular functioni

  • ADP-ribosylation factor binding Source: RGD
  • identical protein binding Source: IntAct
  • PDZ domain binding Source: RGD

GO - Biological processi

  • protein localization Source: RGD
  • signal transduction Source: GO_Central

Names & Taxonomyi

Protein namesi
Recommended name:
General receptor for phosphoinositides 1-associated scaffold protein
Short name:
GRP1-associated scaffold protein
Alternative name(s):
95 kDa postsynaptic density protein discs-large ZO-1 domain-containing protein
PSD-95 PDZ domain-containing protein
Tamalin
Gene namesi
Name:Grasp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi70554. Grasp.

Subcellular locationi

  • Cytoplasmperinuclear region 1 Publication
  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
  • Cell junctionsynapsepostsynaptic cell membrane 1 Publication

  • Note: Localized at postsynaptic membranes.

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
  • plasma membrane Source: RGD
  • postsynaptic membrane Source: UniProtKB-SubCell

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000875861 – 394General receptor for phosphoinositides 1-associated scaffold proteinAdd BLAST394

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei76PhosphothreonineBy similarity1
Modified residuei93PhosphoserineCombined sources1
Modified residuei236PhosphotyrosineBy similarity1
Modified residuei269Omega-N-methylarginineBy similarity1
Modified residuei386PhosphoserineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ8R4T5.
PRIDEiQ8R4T5.

PTM databases

iPTMnetiQ8R4T5.
PhosphoSitePlusiQ8R4T5.

Expressioni

Tissue specificityi

Expressed in brain.1 Publication

Gene expression databases

BgeeiENSRNOG00000007346.
ExpressionAtlasiQ8R4T5. baseline.
GenevisibleiQ8R4T5. RN.

Interactioni

Subunit structurei

Interacts with PSCD3 (By similarity). Heteromer. Composed of GRASP, PSCD2 and at least one GRM1. Also interacts with GRM2, GRM3 and GRM5.By similarity1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • ADP-ribosylation factor binding Source: RGD
  • identical protein binding Source: IntAct
  • PDZ domain binding Source: RGD

Protein-protein interaction databases

BioGridi251380. 14 interactors.
IntActiQ8R4T5. 16 interactors.
MINTiMINT-1790327.
STRINGi10116.ENSRNOP00000009979.

Structurei

Secondary structure

1394
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi98 – 104Combined sources7
Beta strandi107 – 109Combined sources3
Beta strandi112 – 119Combined sources8
Beta strandi130 – 137Combined sources8
Helixi142 – 145Combined sources4
Beta strandi153 – 157Combined sources5
Helixi167 – 176Combined sources10
Turni177 – 179Combined sources3
Beta strandi180 – 186Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EGKX-ray2.85A/B/C/D96-394[»]
2EGNX-ray2.40A96-189[»]
2EGOX-ray1.80A/B96-189[»]
ProteinModelPortaliQ8R4T5.
SMRiQ8R4T5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8R4T5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini100 – 189PDZPROSITE-ProRule annotationAdd BLAST90

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni180 – 257Interaction with PSCD3By similarityAdd BLAST78

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi294 – 323Pro-richAdd BLAST30

Phylogenomic databases

eggNOGiKOG3528. Eukaryota.
ENOG410ZJVP. LUCA.
GeneTreeiENSGT00530000063734.
HOGENOMiHOG000231871.
HOVERGENiHBG053061.
InParanoidiQ8R4T5.
OMAiSVEMCTF.
OrthoDBiEOG091G0NRM.
PhylomeDBiQ8R4T5.
TreeFamiTF316315.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiView protein in InterPro
IPR001478. PDZ.
PfamiView protein in Pfam
PF00595. PDZ. 1 hit.
SMARTiView protein in SMART
SM00228. PDZ. 1 hit.
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiView protein in PROSITE
PS50106. PDZ. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8R4T5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLRRLRKLQ QKEEATAAPD LAGRAPDSEA ARAAPTPSGP PAAAAPPGAP
60 70 80 90 100
GDELYAALED YHPAELYRAL AVSGGTLPRR KGSGFRWKNF TQSPEQQRKV
110 120 130 140 150
LTLEKGDNQT FGFEIQTYGL HHREEQRVEM VTFVCRVHES SPAQLAGLTP
160 170 180 190 200
GDTIASVNGL NVEGIRHREI VDIIKASGNV LRLETLYGTS IRKAELEARL
210 220 230 240 250
QYLKQTLYEK WGEYRSLMVQ EQRLVHGLVV KDPSIYDTLE SVRSCLYGAG
260 270 280 290 300
LLPGSLPFGP LLAAPGGARG GSRRAKGDTD DAVYHTCFFG GAEPQALPPP
310 320 330 340 350
PPPARAPGPG SAETPASVLC PAPRATLSRS ASVRCAGPGG GGGGGAPGAL
360 370 380 390
WTEAREQALC GAGLRKTKYR SFRRRLLKFI PGLNRSLEEE ESQL
Length:394
Mass (Da):42,321
Last modified:June 1, 2002 - v1
Checksum:i520D0AD23CB2B70D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF374272 mRNA. Translation: AAL87038.1.
RefSeqiNP_620249.1. NM_138894.1.
UniGeneiRn.229849.

Genome annotation databases

EnsembliENSRNOT00000009979; ENSRNOP00000009979; ENSRNOG00000007346.
GeneIDi192254.
KEGGirno:192254.
UCSCiRGD:70554. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF374272 mRNA. Translation: AAL87038.1.
RefSeqiNP_620249.1. NM_138894.1.
UniGeneiRn.229849.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EGKX-ray2.85A/B/C/D96-394[»]
2EGNX-ray2.40A96-189[»]
2EGOX-ray1.80A/B96-189[»]
ProteinModelPortaliQ8R4T5.
SMRiQ8R4T5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251380. 14 interactors.
IntActiQ8R4T5. 16 interactors.
MINTiMINT-1790327.
STRINGi10116.ENSRNOP00000009979.

PTM databases

iPTMnetiQ8R4T5.
PhosphoSitePlusiQ8R4T5.

Proteomic databases

PaxDbiQ8R4T5.
PRIDEiQ8R4T5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009979; ENSRNOP00000009979; ENSRNOG00000007346.
GeneIDi192254.
KEGGirno:192254.
UCSCiRGD:70554. rat.

Organism-specific databases

CTDi160622.
RGDi70554. Grasp.

Phylogenomic databases

eggNOGiKOG3528. Eukaryota.
ENOG410ZJVP. LUCA.
GeneTreeiENSGT00530000063734.
HOGENOMiHOG000231871.
HOVERGENiHBG053061.
InParanoidiQ8R4T5.
OMAiSVEMCTF.
OrthoDBiEOG091G0NRM.
PhylomeDBiQ8R4T5.
TreeFamiTF316315.

Miscellaneous databases

EvolutionaryTraceiQ8R4T5.
PROiQ8R4T5.

Gene expression databases

BgeeiENSRNOG00000007346.
ExpressionAtlasiQ8R4T5. baseline.
GenevisibleiQ8R4T5. RN.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiView protein in InterPro
IPR001478. PDZ.
PfamiView protein in Pfam
PF00595. PDZ. 1 hit.
SMARTiView protein in SMART
SM00228. PDZ. 1 hit.
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiView protein in PROSITE
PS50106. PDZ. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGRASP_RAT
AccessioniPrimary (citable) accession number: Q8R4T5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: June 1, 2002
Last modified: March 15, 2017
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.