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Q8R4T5 (GRASP_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
General receptor for phosphoinositides 1-associated scaffold protein

Short name=GRP1-associated scaffold protein
Alternative name(s):
95 kDa postsynaptic density protein discs-large ZO-1 domain-containing protein
PSD-95 PDZ domain-containing protein
Tamalin
Gene names
Name:Grasp
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length394 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in intracellular trafficking and contributes to the macromolecular organization of group 1 metabotropic glutamate receptors (mGluRs) at synapses. Ref.1

Subunit structure

Interacts with PSCD3 By similarity. Heteromer. Composed of GRASP, PSCD2 and at least one GRM1. Also interacts with GRM2, GRM3 and GRM5. Ref.1

Subcellular location

Cytoplasmperinuclear region. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junctionsynapsepostsynaptic cell membrane. Note: Localized at postsynaptic membranes. Ref.1

Tissue specificity

Expressed in brain. Ref.1

Sequence similarities

Contains 1 PDZ (DHR) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 394394General receptor for phosphoinositides 1-associated scaffold protein
PRO_0000087586

Regions

Domain100 – 18990PDZ
Region180 – 25778Interaction with PSCD3 By similarity
Compositional bias294 – 32330Pro-rich

Amino acid modifications

Modified residue2361Phosphotyrosine By similarity

Secondary structure

................. 394
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8R4T5 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 520D0AD23CB2B70D

FASTA39442,321
        10         20         30         40         50         60 
MTLRRLRKLQ QKEEATAAPD LAGRAPDSEA ARAAPTPSGP PAAAAPPGAP GDELYAALED 

        70         80         90        100        110        120 
YHPAELYRAL AVSGGTLPRR KGSGFRWKNF TQSPEQQRKV LTLEKGDNQT FGFEIQTYGL 

       130        140        150        160        170        180 
HHREEQRVEM VTFVCRVHES SPAQLAGLTP GDTIASVNGL NVEGIRHREI VDIIKASGNV 

       190        200        210        220        230        240 
LRLETLYGTS IRKAELEARL QYLKQTLYEK WGEYRSLMVQ EQRLVHGLVV KDPSIYDTLE 

       250        260        270        280        290        300 
SVRSCLYGAG LLPGSLPFGP LLAAPGGARG GSRRAKGDTD DAVYHTCFFG GAEPQALPPP 

       310        320        330        340        350        360 
PPPARAPGPG SAETPASVLC PAPRATLSRS ASVRCAGPGG GGGGGAPGAL WTEAREQALC 

       370        380        390 
GAGLRKTKYR SFRRRLLKFI PGLNRSLEEE ESQL 

« Hide

References

[1]"Tamalin, a PDZ domain-containing protein, links a protein complex formation of group 1 metabotropic glutamate receptors and the guanine nucleotide exchange factor cytohesins."
Kitano J., Kimura K., Yamazaki Y., Soda T., Shigemoto R., Nakajima Y., Nakanishi S.
J. Neurosci. 22:1280-1289(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH GRM1; GRM2; GRM3; GRM5 AND PSCD2, SUBCELLULAR LOCATION, FUNCTION.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF374272 mRNA. Translation: AAL87038.1.
RefSeqNP_620249.1. NM_138894.1.
UniGeneRn.229849.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2EGKX-ray2.85A/B/C/D96-394[»]
2EGNX-ray2.40A96-189[»]
2EGOX-ray1.80A/B96-189[»]
ProteinModelPortalQ8R4T5.
SMRQ8R4T5. Positions 97-191.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid251380. 14 interactions.
IntActQ8R4T5. 16 interactions.
MINTMINT-1790327.
STRING10116.ENSRNOP00000009979.

PTM databases

PhosphoSiteQ8R4T5.

Proteomic databases

PaxDbQ8R4T5.
PRIDEQ8R4T5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000009979; ENSRNOP00000009979; ENSRNOG00000007346.
GeneID192254.
KEGGrno:192254.
UCSCRGD:70554. rat.

Organism-specific databases

CTD160622.
RGD70554. Grasp.

Phylogenomic databases

eggNOGNOG244814.
GeneTreeENSGT00530000063734.
HOGENOMHOG000231871.
HOVERGENHBG053061.
InParanoidQ8R4T5.
OMAEGFRHKE.
OrthoDBEOG72NRQP.
PhylomeDBQ8R4T5.
TreeFamTF316315.

Gene expression databases

GenevestigatorQ8R4T5.

Family and domain databases

Gene3D2.30.42.10. 1 hit.
InterProIPR001478. PDZ.
[Graphical view]
PfamPF00595. PDZ. 1 hit.
[Graphical view]
SMARTSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMSSF50156. SSF50156. 1 hit.
PROSITEPS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ8R4T5.
NextBio622908.
PROQ8R4T5.

Entry information

Entry nameGRASP_RAT
AccessionPrimary (citable) accession number: Q8R4T5
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: June 1, 2002
Last modified: April 16, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references