Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein phosphatase 1 regulatory subunit 14C

Gene

Ppp1r14c

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor of the PP1 regulatory subunit PPP1CA.1 Publication

GO - Molecular functioni

  • protein serine/threonine phosphatase inhibitor activity Source: MGI

GO - Biological processi

  • regulation of phosphorylation Source: InterPro
  • regulation of protein dephosphorylation Source: GO_Central
  • signal transduction Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Protein phosphatase inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory subunit 14C
Alternative name(s):
Kinase-enhanced PP1 inhibitor
PKC-potentiated PP1 inhibitory protein
Gene namesi
Name:Ppp1r14c
Synonyms:Kepi
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1923392. Ppp1r14c.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 164163Protein phosphatase 1 regulatory subunit 14CPRO_0000071495Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei25 – 251PhosphoserineBy similarity
Modified residuei33 – 331PhosphoserineBy similarity
Modified residuei72 – 721Phosphothreonine; by ILK1By similarity

Post-translational modificationi

The main inhibitory site appears to be Thr-72 (By similarity). Has over 600-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.By similarity1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ8R4S0.
PaxDbiQ8R4S0.
PeptideAtlasiQ8R4S0.
PRIDEiQ8R4S0.

PTM databases

iPTMnetiQ8R4S0.
PhosphoSiteiQ8R4S0.

Expressioni

Tissue specificityi

Detected in heart, muscle, spinal cord, hippocampus, hypothalamus, thalamus, midbrain, brain stem, cerebellum, brain cortex and olfactory bulb.1 Publication

Inductioni

Up-regulated by morphine.1 Publication

Gene expression databases

BgeeiQ8R4S0.
GenevisibleiQ8R4S0. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000045110.

Structurei

3D structure databases

ProteinModelPortaliQ8R4S0.
SMRiQ8R4S0. Positions 70-155.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi6 – 3530Gly-richAdd
BLAST
Compositional biasi36 – 438Poly-Ser
Compositional biasi58 – 636Poly-Gln
Compositional biasi101 – 1044Poly-Glu

Sequence similaritiesi

Belongs to the PP1 inhibitor family.Curated

Phylogenomic databases

eggNOGiENOG410IYUT. Eukaryota.
ENOG4112B8C. LUCA.
GeneTreeiENSGT00390000003608.
HOGENOMiHOG000231993.
HOVERGENiHBG054419.
InParanoidiQ8R4S0.
KOiK17556.
OMAiDCHKPTE.
OrthoDBiEOG7RNK21.
PhylomeDBiQ8R4S0.
TreeFamiTF105546.

Family and domain databases

Gene3Di1.10.150.220. 1 hit.
InterProiIPR008025. PP1_inhibitor.
[Graphical view]
PANTHERiPTHR16188. PTHR16188. 1 hit.
PfamiPF05361. PP1_inhibitor. 1 hit.
[Graphical view]
SUPFAMiSSF81790. SSF81790. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R4S0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVVTGGGEA AGGGGGGGAR VFFQSPRGGT GGSPGSSSSS GSSREDSAPV
60 70 80 90 100
TTVAAAGQVQ QQQRRHQQGK VTVKYDRKEL RKRLVLEEWI VEQLGQLYGC
110 120 130 140 150
EEEEMPDVEI DIDDLLDANS EEERASKLQE ALVDCYKPTE EFIRELLSRI
160
RGMRKLSPPQ KKSV
Length:164
Mass (Da):17,754
Last modified:June 1, 2002 - v1
Checksum:i0919DEE0344B691B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF407167 mRNA. Translation: AAL83508.1.
AK085997 mRNA. Translation: BAC39589.1.
BC055717 mRNA. Translation: AAH55717.1.
CCDSiCCDS56669.1.
RefSeqiNP_597844.1. NM_133485.2.
UniGeneiMm.308126.

Genome annotation databases

EnsembliENSMUST00000043374; ENSMUSP00000045110; ENSMUSG00000040653.
GeneIDi76142.
KEGGimmu:76142.
UCSCiuc007ehq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF407167 mRNA. Translation: AAL83508.1.
AK085997 mRNA. Translation: BAC39589.1.
BC055717 mRNA. Translation: AAH55717.1.
CCDSiCCDS56669.1.
RefSeqiNP_597844.1. NM_133485.2.
UniGeneiMm.308126.

3D structure databases

ProteinModelPortaliQ8R4S0.
SMRiQ8R4S0. Positions 70-155.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000045110.

PTM databases

iPTMnetiQ8R4S0.
PhosphoSiteiQ8R4S0.

Proteomic databases

MaxQBiQ8R4S0.
PaxDbiQ8R4S0.
PeptideAtlasiQ8R4S0.
PRIDEiQ8R4S0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043374; ENSMUSP00000045110; ENSMUSG00000040653.
GeneIDi76142.
KEGGimmu:76142.
UCSCiuc007ehq.1. mouse.

Organism-specific databases

CTDi81706.
MGIiMGI:1923392. Ppp1r14c.

Phylogenomic databases

eggNOGiENOG410IYUT. Eukaryota.
ENOG4112B8C. LUCA.
GeneTreeiENSGT00390000003608.
HOGENOMiHOG000231993.
HOVERGENiHBG054419.
InParanoidiQ8R4S0.
KOiK17556.
OMAiDCHKPTE.
OrthoDBiEOG7RNK21.
PhylomeDBiQ8R4S0.
TreeFamiTF105546.

Miscellaneous databases

PROiQ8R4S0.
SOURCEiSearch...

Gene expression databases

BgeeiQ8R4S0.
GenevisibleiQ8R4S0. MM.

Family and domain databases

Gene3Di1.10.150.220. 1 hit.
InterProiIPR008025. PP1_inhibitor.
[Graphical view]
PANTHERiPTHR16188. PTHR16188. 1 hit.
PfamiPF05361. PP1_inhibitor. 1 hit.
[Graphical view]
SUPFAMiSSF81790. SSF81790. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "KEPI, a PKC-dependent protein phosphatase 1 inhibitor regulated by morphine."
    Liu Q.-R., Zhang P.-W., Zhen Q., Walther D., Wang X.-B., Uhl G.
    J. Biol. Chem. 277:13312-13320(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, PHOSPHORYLATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
    Strain: C57BL/6J.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Heart.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Heart and Lung.

Entry informationi

Entry nameiPP14C_MOUSE
AccessioniPrimary (citable) accession number: Q8R4S0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 9, 2004
Last sequence update: June 1, 2002
Last modified: July 6, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.