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Protein

Protein phosphatase 1 regulatory subunit 14C

Gene

Ppp1r14c

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor of the PP1 regulatory subunit PPP1CA.

GO - Molecular functioni

  • protein serine/threonine phosphatase inhibitor activity Source: RGD

GO - Biological processi

  • negative regulation of catalytic activity Source: GOC
  • regulation of phosphorylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Protein phosphatase inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory subunit 14C
Alternative name(s):
Kinase-enhanced PP1 inhibitor
PKC-potentiated PP1 inhibitory protein
Gene namesi
Name:Ppp1r14c
Synonyms:Kepi
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi620538. Ppp1r14c.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: InterPro
  • endomembrane system Source: UniProtKB-SubCell
  • membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 164163Protein phosphatase 1 regulatory subunit 14CPRO_0000071496Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei72 – 721Phosphothreonine; by ILK1By similarity

Post-translational modificationi

Has over 600-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction. The main inhibitory site appears to be Thr-72 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiQ8R4R9.

PTM databases

PhosphoSiteiQ8R4R9.

Expressioni

Gene expression databases

GenevestigatoriQ8R4R9.

Structurei

3D structure databases

ProteinModelPortaliQ8R4R9.
SMRiQ8R4R9. Positions 62-155.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi58 – 636Poly-Gln
Compositional biasi101 – 1044Poly-Glu

Sequence similaritiesi

Belongs to the PP1 inhibitor family.Curated

Phylogenomic databases

eggNOGieuNOG04467.
GeneTreeiENSGT00390000003608.
HOGENOMiHOG000231993.
HOVERGENiHBG054419.
InParanoidiQ8R4R9.
KOiK17556.
OMAiDCHKPTE.
OrthoDBiEOG7RNK21.
PhylomeDBiQ8R4R9.
TreeFamiTF105546.

Family and domain databases

Gene3Di1.10.150.220. 1 hit.
InterProiIPR008025. PP1_inhibitor.
[Graphical view]
PANTHERiPTHR16188. PTHR16188. 1 hit.
PfamiPF05361. PP1_inhibitor. 1 hit.
[Graphical view]
SUPFAMiSSF81790. SSF81790. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R4R9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVVTGGGEA AGGTSGGGAR VFFQSPRGGT GGSRESSSHS GSSREDSAPV
60 70 80 90 100
ATVAAAGQVQ QQQRRHQQGK VTVKYDRKEL RKRLVLEEWI VEQLGQLYGC
110 120 130 140 150
EEEEMPDVEI DIDDLLDADS EEERASKLQE ALVDCYKPTE EFIRELLSRI
160
RGMRKLSPPQ KKSV
Length:164
Mass (Da):17,980
Last modified:June 1, 2002 - v1
Checksum:i2E48BCB85446A4DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF407168 mRNA. Translation: AAL83509.1.
BC086978 mRNA. Translation: AAH86978.1.
RefSeqiNP_596916.1. NM_133425.3.
UniGeneiRn.87667.

Genome annotation databases

EnsembliENSRNOT00000022046; ENSRNOP00000022046; ENSRNOG00000016368.
GeneIDi171010.
KEGGirno:171010.
UCSCiRGD:620538. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF407168 mRNA. Translation: AAL83509.1.
BC086978 mRNA. Translation: AAH86978.1.
RefSeqiNP_596916.1. NM_133425.3.
UniGeneiRn.87667.

3D structure databases

ProteinModelPortaliQ8R4R9.
SMRiQ8R4R9. Positions 62-155.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ8R4R9.

Proteomic databases

PRIDEiQ8R4R9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022046; ENSRNOP00000022046; ENSRNOG00000016368.
GeneIDi171010.
KEGGirno:171010.
UCSCiRGD:620538. rat.

Organism-specific databases

CTDi81706.
RGDi620538. Ppp1r14c.

Phylogenomic databases

eggNOGieuNOG04467.
GeneTreeiENSGT00390000003608.
HOGENOMiHOG000231993.
HOVERGENiHBG054419.
InParanoidiQ8R4R9.
KOiK17556.
OMAiDCHKPTE.
OrthoDBiEOG7RNK21.
PhylomeDBiQ8R4R9.
TreeFamiTF105546.

Miscellaneous databases

NextBioi621491.
PROiQ8R4R9.

Gene expression databases

GenevestigatoriQ8R4R9.

Family and domain databases

Gene3Di1.10.150.220. 1 hit.
InterProiIPR008025. PP1_inhibitor.
[Graphical view]
PANTHERiPTHR16188. PTHR16188. 1 hit.
PfamiPF05361. PP1_inhibitor. 1 hit.
[Graphical view]
SUPFAMiSSF81790. SSF81790. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "KEPI, a PKC-dependent protein phosphatase 1 inhibitor regulated by morphine."
    Liu Q.-R., Zhang P.-W., Zhen Q., Walther D., Wang X.-B., Uhl G.
    J. Biol. Chem. 277:13312-13320(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  3. Lubec G., Kang S.U., Lubec S.
    Submitted (SEP-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 35-44, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiPP14C_RAT
AccessioniPrimary (citable) accession number: Q8R4R9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 9, 2004
Last sequence update: June 1, 2002
Last modified: March 4, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.