Q8R4R9 (PP14C_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 1 regulatory subunit 14C

Alternative name(s):
Kinase-enhanced PP1 inhibitor
PKC-potentiated PP1 inhibitory protein

Gene names

Name:	Ppp1r14c
Synonyms:	Kepi

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	164 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibitor of the PP1 regulatory subunit PPP1CA.
Subcellular location	Endomembrane system; Peripheral membrane protein By similarity.
Post-translational modification	Has over 600-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction. The main inhibitory site appears to be Thr-72 By similarity.
Sequence similarities	Belongs to the PP1 inhibitor family.

Ontologies

Keywords
Cellular component	Membrane
Molecular function	Protein phosphatase inhibitor
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	regulation of phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro endomembrane system Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	protein serine/threonine phosphatase inhibitor activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 164	164	Protein phosphatase 1 regulatory subunit 14C		PRO_0000071496
Regions
	Compositional bias	58 – 63	6	Poly-Gln
	Compositional bias	101 – 104	4	Poly-Glu
Amino acid modifications
	Modified residue	72	1	Phosphothreonine; by ILK1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8R4R9 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 2E48BCB85446A4DE	FASTA	164	17,980
	10 20 30 40 50 60 MSVVTGGGEA AGGTSGGGAR VFFQSPRGGT GGSRESSSHS GSSREDSAPV ATVAAAGQVQ 70 80 90 100 110 120 QQQRRHQQGK VTVKYDRKEL RKRLVLEEWI VEQLGQLYGC EEEEMPDVEI DIDDLLDADS 130 140 150 160 EEERASKLQE ALVDCYKPTE EFIRELLSRI RGMRKLSPPQ KKSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"KEPI, a PKC-dependent protein phosphatase 1 inhibitor regulated by morphine." Liu Q.-R., Zhang P.-W., Zhen Q., Walther D., Wang X.-B., Uhl G. J. Biol. Chem. 277:13312-13320(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.
[3]	Lubec G., Kang S.U., Lubec S. Submitted (SEP-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 35-44, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF407168 mRNA. Translation: AAL83509.1. BC086978 mRNA. Translation: AAH86978.1.
IPI	IPI00201092.
RefSeq	NP_596916.1. NM_133425.3.
UniGene	Rn.87667.
3D structure databases
ProteinModelPortal	Q8R4R9.
SMR	Q8R4R9. Positions 62-155.
ModBase	Search...
PTM databases
PhosphoSite	Q8R4R9.
Proteomic databases
PRIDE	Q8R4R9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000022046; ENSRNOP00000022046; ENSRNOG00000016368.
GeneID	171010.
KEGG	rno:171010.
UCSC	RGD:620538. rat.
Organism-specific databases
CTD	81706.
RGD	620538. Ppp1r14c.
Phylogenomic databases
eggNOG	euNOG04467.
GeneTree	ENSGT00390000003608.
HOGENOM	HOG000231993.
HOVERGEN	HBG054419.
InParanoid	Q8R4R9.
OMA	XEEEMPD.
OrthoDB	EOG4BVRW6.
Gene expression databases
Genevestigator	Q8R4R9.
GermOnline	ENSRNOG00000016368. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.150.220. 1 hit.
InterPro	IPR008025. PP1_inhibitor. [Graphical view]
PANTHER	PTHR16188. PTHR16188. 1 hit.
Pfam	PF05361. PP1_inhibitor. 1 hit. [Graphical view]
SUPFAM	SSF81790. PP1_inhibitor. 1 hit.
ProtoNet	Search...
Other
NextBio	621491.

Entry information

Entry name

PP14C_RAT

Accession

Primary (citable) accession number: Q8R4R9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 9, 2004
Last sequence update:	June 1, 2002
Last modified:	April 3, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program