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Protein

Nucleoporin NUP53

Gene

Nup35

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

ReactomeiR-MMU-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-MMU-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-MMU-191859. snRNP Assembly.
R-MMU-3108214. SUMOylation of DNA damage response and repair proteins.
R-MMU-3301854. Nuclear Pore Complex (NPC) Disassembly.
R-MMU-3371453. Regulation of HSF1-mediated heat shock response.
R-MMU-4570464. SUMOylation of RNA binding proteins.
R-MMU-4615885. SUMOylation of DNA replication proteins.
R-MMU-5578749. Transcriptional regulation by small RNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin NUP53
Alternative name(s):
35 kDa nucleoporin
Mitotic phosphoprotein 44
Short name:
MP-44
Nuclear pore complex protein Nup53
Nucleoporin Nup35
Gene namesi
Name:Nup35
Synonyms:Mp44, Nup53
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componentsi: Chromosome 2, Chromosome 7

Organism-specific databases

MGIiMGI:1916732. Nup35.

Subcellular locationi

  • Nucleusnuclear pore complex By similarity
  • Nucleus membrane By similarity; Peripheral membrane protein By similarity

  • Note: Tightly associated with the nuclear membrane and lamina.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002342951 – 325Nucleoporin NUP53Add BLAST325

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53PhosphoserineBy similarity1
Modified residuei55PhosphoserineBy similarity1
Modified residuei66PhosphoserineCombined sources1
Modified residuei73PhosphoserineBy similarity1
Modified residuei99PhosphoserineBy similarity1
Modified residuei100PhosphoserineCombined sources1
Modified residuei106PhosphothreonineBy similarity1
Modified residuei121PhosphoserineBy similarity1
Modified residuei128PhosphothreonineBy similarity1
Modified residuei137PhosphoserineBy similarity1
Modified residuei251PhosphoserineCombined sources1
Modified residuei258PhosphoserineCombined sources1
Modified residuei264PhosphothreonineBy similarity1
Modified residuei265PhosphothreonineCombined sources1
Modified residuei272PhosphothreonineBy similarity1
Modified residuei274PhosphothreonineBy similarity1
Modified residuei278PhosphoserineBy similarity1
Modified residuei279PhosphothreonineBy similarity1
Modified residuei283PhosphoserineBy similarity1
Modified residuei307PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8R4R6.
MaxQBiQ8R4R6.
PaxDbiQ8R4R6.
PeptideAtlasiQ8R4R6.
PRIDEiQ8R4R6.

PTM databases

iPTMnetiQ8R4R6.
PhosphoSitePlusiQ8R4R6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026999.
CleanExiMM_NUP35.
ExpressionAtlasiQ8R4R6. baseline and differential.
GenevisibleiQ8R4R6. MM.

Interactioni

Subunit structurei

Interacts with TMEM48/NDC1. Forms a complex with NUP93, NUP155, NUP205 and lamin B; The interaction with NUP93 is direct.By similarity

GO - Molecular functioni

  • protein homodimerization activity Source: MGI

Protein-protein interaction databases

IntActiQ8R4R6. 2 interactors.
MINTiMINT-4127456.
STRINGi10090.ENSMUSP00000107385.

Structurei

Secondary structure

1325
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi170 – 173Combined sources4
Beta strandi174 – 178Combined sources5
Helixi182 – 184Combined sources3
Helixi185 – 193Combined sources9
Beta strandi198 – 203Combined sources6
Beta strandi205 – 216Combined sources12
Helixi217 – 224Combined sources8
Turni225 – 228Combined sources4
Turni232 – 234Combined sources3
Beta strandi238 – 241Combined sources4
Helixi245 – 248Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WWHX-ray2.70A/B/C/D156-261[»]
ProteinModelPortaliQ8R4R6.
SMRiQ8R4R6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8R4R6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini169 – 249RRM Nup35-typePROSITE-ProRule annotationAdd BLAST81

Sequence similaritiesi

Belongs to the Nup53 family.Curated
Contains 1 RRM Nup35-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4285. Eukaryota.
ENOG4111ARE. LUCA.
GeneTreeiENSGT00390000005923.
HOGENOMiHOG000231921.
HOVERGENiHBG060396.
InParanoidiQ8R4R6.
KOiK14313.
OMAiSSYHTND.
OrthoDBiEOG091G0DAQ.
PhylomeDBiQ8R4R6.
TreeFamiTF325369.

Family and domain databases

InterProiIPR017389. Nucleoporin_NUP53.
IPR012677. Nucleotide-bd_a/b_plait.
IPR007846. RRM_NUP35_dom.
[Graphical view]
PfamiPF05172. Nup35_RRM. 1 hit.
[Graphical view]
PIRSFiPIRSF038119. Nucleoporin_NUP53. 1 hit.
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS51472. RRM_NUP35. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R4R6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAFAVDPQA PTLGSEPMML GSPTSPKTGA NAQFLPGFLM GDLPAPVTPQ
60 70 80 90 100
PRSISGPSVG VMEMRSPLLA GGSPPQPVVP AHKDKSGAPP VRSIYDDISS
110 120 130 140 150
PGLGSTPLTS RRQANISLLQ SPLVGATTPV PGQSMFSPAN IGQPRKTTLS
160 170 180 190 200
PAQLDPFYTQ GDSLTSEDHL DDTWVTVFGF PQASASYILL QFAQYGNILK
210 220 230 240 250
HVMSNTGNWM HIRYQSKLQA RKALSKDGRI FGESIMIGVK PCIDKNVMEN
260 270 280 290 300
SDRGVLSSPS LAFTTPIRTL GTPTQSGSTP RVSTMRPLAT AYKASTSDYQ
310 320
VISDRQTPKK DESLVSRAME YMFGW
Length:325
Mass (Da):34,786
Last modified:May 16, 2006 - v2
Checksum:iA8994CBDF25A3C84
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13L → V in AAL86380 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF411517 mRNA. Translation: AAL86380.1.
AK009187 mRNA. Translation: BAB26128.1.
AK089997 mRNA. Translation: BAC41034.1.
AK136109 mRNA. Translation: BAE22825.1.
AK145775 mRNA. Translation: BAE26644.1.
AL928869 Genomic DNA. Translation: CAM23081.1.
BC048814 mRNA. Translation: AAH48814.1.
CCDSiCCDS16179.1.
RefSeqiNP_001177108.1. NM_001190179.1.
NP_081367.1. NM_027091.4.
UniGeneiMm.29200.

Genome annotation databases

EnsembliENSMUST00000028382; ENSMUSP00000028382; ENSMUSG00000026999.
ENSMUST00000111755; ENSMUSP00000107385; ENSMUSG00000091900.
GeneIDi69482.
KEGGimmu:69482.
UCSCiuc008khs.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF411517 mRNA. Translation: AAL86380.1.
AK009187 mRNA. Translation: BAB26128.1.
AK089997 mRNA. Translation: BAC41034.1.
AK136109 mRNA. Translation: BAE22825.1.
AK145775 mRNA. Translation: BAE26644.1.
AL928869 Genomic DNA. Translation: CAM23081.1.
BC048814 mRNA. Translation: AAH48814.1.
CCDSiCCDS16179.1.
RefSeqiNP_001177108.1. NM_001190179.1.
NP_081367.1. NM_027091.4.
UniGeneiMm.29200.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WWHX-ray2.70A/B/C/D156-261[»]
ProteinModelPortaliQ8R4R6.
SMRiQ8R4R6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8R4R6. 2 interactors.
MINTiMINT-4127456.
STRINGi10090.ENSMUSP00000107385.

PTM databases

iPTMnetiQ8R4R6.
PhosphoSitePlusiQ8R4R6.

Proteomic databases

EPDiQ8R4R6.
MaxQBiQ8R4R6.
PaxDbiQ8R4R6.
PeptideAtlasiQ8R4R6.
PRIDEiQ8R4R6.

Protocols and materials databases

DNASUi69482.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028382; ENSMUSP00000028382; ENSMUSG00000026999.
ENSMUST00000111755; ENSMUSP00000107385; ENSMUSG00000091900.
GeneIDi69482.
KEGGimmu:69482.
UCSCiuc008khs.2. mouse.

Organism-specific databases

CTDi129401.
MGIiMGI:1916732. Nup35.

Phylogenomic databases

eggNOGiKOG4285. Eukaryota.
ENOG4111ARE. LUCA.
GeneTreeiENSGT00390000005923.
HOGENOMiHOG000231921.
HOVERGENiHBG060396.
InParanoidiQ8R4R6.
KOiK14313.
OMAiSSYHTND.
OrthoDBiEOG091G0DAQ.
PhylomeDBiQ8R4R6.
TreeFamiTF325369.

Enzyme and pathway databases

ReactomeiR-MMU-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-MMU-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-MMU-191859. snRNP Assembly.
R-MMU-3108214. SUMOylation of DNA damage response and repair proteins.
R-MMU-3301854. Nuclear Pore Complex (NPC) Disassembly.
R-MMU-3371453. Regulation of HSF1-mediated heat shock response.
R-MMU-4570464. SUMOylation of RNA binding proteins.
R-MMU-4615885. SUMOylation of DNA replication proteins.
R-MMU-5578749. Transcriptional regulation by small RNAs.

Miscellaneous databases

EvolutionaryTraceiQ8R4R6.
PROiQ8R4R6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026999.
CleanExiMM_NUP35.
ExpressionAtlasiQ8R4R6. baseline and differential.
GenevisibleiQ8R4R6. MM.

Family and domain databases

InterProiIPR017389. Nucleoporin_NUP53.
IPR012677. Nucleotide-bd_a/b_plait.
IPR007846. RRM_NUP35_dom.
[Graphical view]
PfamiPF05172. Nup35_RRM. 1 hit.
[Graphical view]
PIRSFiPIRSF038119. Nucleoporin_NUP53. 1 hit.
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS51472. RRM_NUP35. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUP53_MOUSE
AccessioniPrimary (citable) accession number: Q8R4R6
Secondary accession number(s): A2ATJ3, Q9D7J2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: May 16, 2006
Last modified: November 30, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.