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Q8R4R6 (NUP53_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nucleoporin NUP53
Alternative name(s):
35 kDa nucleoporin
Mitotic phosphoprotein 44
Short name=MP-44
Nuclear pore complex protein Nup53
Nucleoporin Nup35
Gene names
Name:Nup35
Synonyms:Mp44, Nup53
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length325 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC By similarity.

Subunit structure

Interacts with TMEM48/NDC1 By similarity. Forms a complex with NUP93, NUP155, NUP205 and lamin B; The interaction with NUP93 is direct By similarity.

Subcellular location

Nucleusnuclear pore complex By similarity. Nucleus membrane; Peripheral membrane protein By similarity. Note: Tightly associated with the nuclear membrane and lamina By similarity.

Sequence similarities

Belongs to the Nup53 family.

Contains 1 RRM Nup35-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 325325Nucleoporin NUP53
PRO_0000234295

Regions

Domain169 – 24981RRM Nup35-type

Amino acid modifications

Modified residue661Phosphoserine By similarity
Modified residue731Phosphoserine By similarity
Modified residue991Phosphoserine By similarity
Modified residue1001Phosphoserine By similarity
Modified residue1061Phosphothreonine By similarity
Modified residue1211Phosphoserine By similarity
Modified residue1281Phosphothreonine By similarity
Modified residue1371Phosphoserine By similarity
Modified residue2581Phosphoserine Ref.6
Modified residue2641Phosphothreonine By similarity
Modified residue2721Phosphothreonine By similarity
Modified residue2781Phosphoserine By similarity
Modified residue2791Phosphothreonine By similarity
Modified residue3071Phosphothreonine By similarity

Experimental info

Sequence conflict131L → V in AAL86380. Ref.1

Secondary structure

................... 325
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8R4R6 [UniParc].

Last modified May 16, 2006. Version 2.
Checksum: A8994CBDF25A3C84

FASTA32534,786
        10         20         30         40         50         60 
MAAFAVDPQA PTLGSEPMML GSPTSPKTGA NAQFLPGFLM GDLPAPVTPQ PRSISGPSVG 

        70         80         90        100        110        120 
VMEMRSPLLA GGSPPQPVVP AHKDKSGAPP VRSIYDDISS PGLGSTPLTS RRQANISLLQ 

       130        140        150        160        170        180 
SPLVGATTPV PGQSMFSPAN IGQPRKTTLS PAQLDPFYTQ GDSLTSEDHL DDTWVTVFGF 

       190        200        210        220        230        240 
PQASASYILL QFAQYGNILK HVMSNTGNWM HIRYQSKLQA RKALSKDGRI FGESIMIGVK 

       250        260        270        280        290        300 
PCIDKNVMEN SDRGVLSSPS LAFTTPIRTL GTPTQSGSTP RVSTMRPLAT AYKASTSDYQ 

       310        320 
VISDRQTPKK DESLVSRAME YMFGW 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and expression analysis of human mitotic phosphoprotein 44 gene."
Guo J.H., Yu L.
Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: BALB/c.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Egg, Submandibular gland and Tongue.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[5]Lubec G., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 214-221, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: OF1.
Tissue: Hippocampus.
[6]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-258, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Crystal structure of the mppn domain of mouse nup35."
Handa N., Murayama K., Kukimoto M., Hamana H., Uchikubo T., Takemoto C., Terada T., Shirouzu M., Yokoyama S.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 156-261.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF411517 mRNA. Translation: AAL86380.1.
AK009187 mRNA. Translation: BAB26128.1.
AK089997 mRNA. Translation: BAC41034.1.
AK136109 mRNA. Translation: BAE22825.1.
AK145775 mRNA. Translation: BAE26644.1.
AL928869 Genomic DNA. Translation: CAM23081.1.
BC048814 mRNA. Translation: AAH48814.1.
RefSeqNP_001177108.1. NM_001190179.1.
NP_081367.1. NM_027091.4.
UniGeneMm.29200.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1WWHX-ray2.70A/B/C/D156-261[»]
ProteinModelPortalQ8R4R6.
SMRQ8R4R6. Positions 169-249.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8R4R6. 2 interactions.
MINTMINT-4127456.
STRING10090.ENSMUSP00000028382.

PTM databases

PhosphoSiteQ8R4R6.

Proteomic databases

PaxDbQ8R4R6.
PRIDEQ8R4R6.

Protocols and materials databases

DNASU69482.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000028382; ENSMUSP00000028382; ENSMUSG00000026999.
ENSMUST00000111755; ENSMUSP00000107385; ENSMUSG00000091900.
GeneID69482.
KEGGmmu:69482.
UCSCuc008khs.2. mouse.

Organism-specific databases

CTD129401.
MGIMGI:1916732. Nup35.

Phylogenomic databases

eggNOGNOG253574.
GeneTreeENSGT00390000005923.
HOGENOMHOG000231921.
HOVERGENHBG060396.
InParanoidA2ATJ3.
KOK14313.
OMAANISVMQ.
OrthoDBEOG79KPFQ.
PhylomeDBQ8R4R6.
TreeFamTF325369.

Gene expression databases

BgeeQ8R4R6.
CleanExMM_NUP35.
GenevestigatorQ8R4R6.

Family and domain databases

InterProIPR017389. Nucleoporin_NUP53.
IPR007846. RRM_NUP35_dom.
[Graphical view]
PANTHERPTHR21527:SF3. PTHR21527:SF3. 1 hit.
PfamPF05172. Nup35_RRM. 1 hit.
[Graphical view]
PIRSFPIRSF038119. Nucleoporin_NUP53. 1 hit.
PROSITEPS51472. RRM_NUP35. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ8R4R6.
NextBio329578.
PROQ8R4R6.
SOURCESearch...

Entry information

Entry nameNUP53_MOUSE
AccessionPrimary (citable) accession number: Q8R4R6
Secondary accession number(s): A2ATJ3, Q9D7J2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: May 16, 2006
Last modified: April 16, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot