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Q8R4R6

- NUP53_MOUSE

UniProt

Q8R4R6 - NUP53_MOUSE

Protein

Nucleoporin NUP53

Gene

Nup35

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 2 (16 May 2006)
      Previous versions | rss
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    Functioni

    Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC By similarity.By similarity

    GO - Biological processi

    1. mRNA transport Source: UniProtKB-KW
    2. protein transport Source: UniProtKB-KW
    3. transmembrane transport Source: InterPro

    Keywords - Biological processi

    mRNA transport, Protein transport, Translocation, Transport

    Enzyme and pathway databases

    ReactomeiREACT_196650. Nuclear Pore Complex (NPC) Disassembly.
    REACT_204192. Regulation of Glucokinase by Glucokinase Regulatory Protein.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nucleoporin NUP53
    Alternative name(s):
    35 kDa nucleoporin
    Mitotic phosphoprotein 44
    Short name:
    MP-44
    Nuclear pore complex protein Nup53
    Nucleoporin Nup35
    Gene namesi
    Name:Nup35
    Synonyms:Mp44, Nup53
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2, UP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1916732. Nup35.

    Subcellular locationi

    Nucleusnuclear pore complex By similarity. Nucleus membrane By similarity; Peripheral membrane protein By similarity
    Note: Tightly associated with the nuclear membrane and lamina.By similarity

    GO - Cellular componenti

    1. nuclear membrane Source: UniProtKB-SubCell
    2. nuclear pore Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Nuclear pore complex, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 325325Nucleoporin NUP53PRO_0000234295Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei66 – 661PhosphoserineBy similarity
    Modified residuei73 – 731PhosphoserineBy similarity
    Modified residuei99 – 991PhosphoserineBy similarity
    Modified residuei100 – 1001PhosphoserineBy similarity
    Modified residuei106 – 1061PhosphothreonineBy similarity
    Modified residuei121 – 1211PhosphoserineBy similarity
    Modified residuei128 – 1281PhosphothreonineBy similarity
    Modified residuei137 – 1371PhosphoserineBy similarity
    Modified residuei258 – 2581Phosphoserine1 Publication
    Modified residuei264 – 2641PhosphothreonineBy similarity
    Modified residuei272 – 2721PhosphothreonineBy similarity
    Modified residuei278 – 2781PhosphoserineBy similarity
    Modified residuei279 – 2791PhosphothreonineBy similarity
    Modified residuei307 – 3071PhosphothreonineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ8R4R6.
    PRIDEiQ8R4R6.

    PTM databases

    PhosphoSiteiQ8R4R6.

    Expressioni

    Gene expression databases

    BgeeiQ8R4R6.
    CleanExiMM_NUP35.
    GenevestigatoriQ8R4R6.

    Interactioni

    Subunit structurei

    Interacts with TMEM48/NDC1. Forms a complex with NUP93, NUP155, NUP205 and lamin B; The interaction with NUP93 is direct.By similarity

    Protein-protein interaction databases

    IntActiQ8R4R6. 2 interactions.
    MINTiMINT-4127456.
    STRINGi10090.ENSMUSP00000028382.

    Structurei

    Secondary structure

    1
    325
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi170 – 1734
    Beta strandi174 – 1785
    Helixi182 – 1843
    Helixi185 – 1939
    Beta strandi198 – 2036
    Beta strandi205 – 21612
    Helixi217 – 2248
    Turni225 – 2284
    Turni232 – 2343
    Beta strandi238 – 2414
    Helixi245 – 2484

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1WWHX-ray2.70A/B/C/D156-261[»]
    ProteinModelPortaliQ8R4R6.
    SMRiQ8R4R6. Positions 169-249.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8R4R6.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini169 – 24981RRM Nup35-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Nup53 family.Curated
    Contains 1 RRM Nup35-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG253574.
    GeneTreeiENSGT00390000005923.
    HOGENOMiHOG000231921.
    HOVERGENiHBG060396.
    InParanoidiA2ATJ3.
    KOiK14313.
    OMAiANISVMQ.
    OrthoDBiEOG79KPFQ.
    PhylomeDBiQ8R4R6.
    TreeFamiTF325369.

    Family and domain databases

    InterProiIPR017389. Nucleoporin_NUP53.
    IPR007846. RRM_NUP35_dom.
    [Graphical view]
    PfamiPF05172. Nup35_RRM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038119. Nucleoporin_NUP53. 1 hit.
    PROSITEiPS51472. RRM_NUP35. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8R4R6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAFAVDPQA PTLGSEPMML GSPTSPKTGA NAQFLPGFLM GDLPAPVTPQ    50
    PRSISGPSVG VMEMRSPLLA GGSPPQPVVP AHKDKSGAPP VRSIYDDISS 100
    PGLGSTPLTS RRQANISLLQ SPLVGATTPV PGQSMFSPAN IGQPRKTTLS 150
    PAQLDPFYTQ GDSLTSEDHL DDTWVTVFGF PQASASYILL QFAQYGNILK 200
    HVMSNTGNWM HIRYQSKLQA RKALSKDGRI FGESIMIGVK PCIDKNVMEN 250
    SDRGVLSSPS LAFTTPIRTL GTPTQSGSTP RVSTMRPLAT AYKASTSDYQ 300
    VISDRQTPKK DESLVSRAME YMFGW 325
    Length:325
    Mass (Da):34,786
    Last modified:May 16, 2006 - v2
    Checksum:iA8994CBDF25A3C84
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti13 – 131L → V in AAL86380. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF411517 mRNA. Translation: AAL86380.1.
    AK009187 mRNA. Translation: BAB26128.1.
    AK089997 mRNA. Translation: BAC41034.1.
    AK136109 mRNA. Translation: BAE22825.1.
    AK145775 mRNA. Translation: BAE26644.1.
    AL928869 Genomic DNA. Translation: CAM23081.1.
    BC048814 mRNA. Translation: AAH48814.1.
    CCDSiCCDS16179.1.
    RefSeqiNP_001177108.1. NM_001190179.1.
    NP_081367.1. NM_027091.4.
    UniGeneiMm.29200.

    Genome annotation databases

    EnsembliENSMUST00000028382; ENSMUSP00000028382; ENSMUSG00000026999.
    ENSMUST00000111755; ENSMUSP00000107385; ENSMUSG00000091900.
    GeneIDi69482.
    KEGGimmu:69482.
    UCSCiuc008khs.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF411517 mRNA. Translation: AAL86380.1 .
    AK009187 mRNA. Translation: BAB26128.1 .
    AK089997 mRNA. Translation: BAC41034.1 .
    AK136109 mRNA. Translation: BAE22825.1 .
    AK145775 mRNA. Translation: BAE26644.1 .
    AL928869 Genomic DNA. Translation: CAM23081.1 .
    BC048814 mRNA. Translation: AAH48814.1 .
    CCDSi CCDS16179.1.
    RefSeqi NP_001177108.1. NM_001190179.1.
    NP_081367.1. NM_027091.4.
    UniGenei Mm.29200.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1WWH X-ray 2.70 A/B/C/D 156-261 [» ]
    ProteinModelPortali Q8R4R6.
    SMRi Q8R4R6. Positions 169-249.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8R4R6. 2 interactions.
    MINTi MINT-4127456.
    STRINGi 10090.ENSMUSP00000028382.

    PTM databases

    PhosphoSitei Q8R4R6.

    Proteomic databases

    PaxDbi Q8R4R6.
    PRIDEi Q8R4R6.

    Protocols and materials databases

    DNASUi 69482.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028382 ; ENSMUSP00000028382 ; ENSMUSG00000026999 .
    ENSMUST00000111755 ; ENSMUSP00000107385 ; ENSMUSG00000091900 .
    GeneIDi 69482.
    KEGGi mmu:69482.
    UCSCi uc008khs.2. mouse.

    Organism-specific databases

    CTDi 129401.
    MGIi MGI:1916732. Nup35.

    Phylogenomic databases

    eggNOGi NOG253574.
    GeneTreei ENSGT00390000005923.
    HOGENOMi HOG000231921.
    HOVERGENi HBG060396.
    InParanoidi A2ATJ3.
    KOi K14313.
    OMAi ANISVMQ.
    OrthoDBi EOG79KPFQ.
    PhylomeDBi Q8R4R6.
    TreeFami TF325369.

    Enzyme and pathway databases

    Reactomei REACT_196650. Nuclear Pore Complex (NPC) Disassembly.
    REACT_204192. Regulation of Glucokinase by Glucokinase Regulatory Protein.

    Miscellaneous databases

    EvolutionaryTracei Q8R4R6.
    NextBioi 329578.
    PROi Q8R4R6.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8R4R6.
    CleanExi MM_NUP35.
    Genevestigatori Q8R4R6.

    Family and domain databases

    InterProi IPR017389. Nucleoporin_NUP53.
    IPR007846. RRM_NUP35_dom.
    [Graphical view ]
    Pfami PF05172. Nup35_RRM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038119. Nucleoporin_NUP53. 1 hit.
    PROSITEi PS51472. RRM_NUP35. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression analysis of human mitotic phosphoprotein 44 gene."
      Guo J.H., Yu L.
      Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: BALB/c.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Egg, Submandibular gland and Tongue.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    5. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 214-221, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.
    6. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-258, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Crystal structure of the mppn domain of mouse nup35."
      Handa N., Murayama K., Kukimoto M., Hamana H., Uchikubo T., Takemoto C., Terada T., Shirouzu M., Yokoyama S.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 156-261.

    Entry informationi

    Entry nameiNUP53_MOUSE
    AccessioniPrimary (citable) accession number: Q8R4R6
    Secondary accession number(s): A2ATJ3, Q9D7J2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2006
    Last sequence update: May 16, 2006
    Last modified: October 1, 2014
    This is version 88 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3