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Protein

Neuropilin and tolloid-like protein 1

Gene

Neto1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the development and/or maintenance of neuronal circuitry. Accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR) critical for maintaining the abundance of GRIN2A-containing NMDARs in the postsynaptic density. Regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, at least in the context of spatial learning and memory.2 Publications

GO - Molecular functioni

  • ionotropic glutamate receptor binding Source: MGI

GO - Biological processi

  • memory Source: UniProtKB
  • positive regulation of excitatory postsynaptic potential Source: MGI
  • receptor localization to synapse Source: MGI
  • regulation of long-term neuronal synaptic plasticity Source: UniProtKB
  • visual learning Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Names & Taxonomyi

Protein namesi
Recommended name:
Neuropilin and tolloid-like protein 1
Alternative name(s):
Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1
Gene namesi
Name:Neto1
Synonyms:Btcl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:2180216. Neto1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 344322ExtracellularSequence analysisAdd
BLAST
Transmembranei345 – 36521HelicalSequence analysisAdd
BLAST
Topological domaini366 – 533168CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • excitatory synapse Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • postsynaptic density Source: UniProtKB
  • postsynaptic membrane Source: UniProtKB-KW
  • synapse Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Depressed long-term potentiation (LTP) at Schaffer collateral-CA1 synapses, NMDAR-dependent spatial learning and memory. Rescued by the ampakine CX546 at physiological doses.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 533511Neuropilin and tolloid-like protein 1PRO_0000021800Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi41 ↔ 68By similarity
Disulfide bondi96 ↔ 118By similarity
Disulfide bondi172 ↔ 202By similarity
Disulfide bondi229 ↔ 251By similarity
Disulfide bondi292 ↔ 304By similarity
Disulfide bondi299 ↔ 317By similarity
Glycosylationi306 – 3061N-linked (GlcNAc...)Sequence analysis
Disulfide bondi311 ↔ 326By similarity
Glycosylationi340 – 3401N-linked (GlcNAc...)Sequence analysis
Modified residuei417 – 4171PhosphotyrosineCombined sources

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ8R4I7.
PaxDbiQ8R4I7.
PeptideAtlasiQ8R4I7.
PRIDEiQ8R4I7.

PTM databases

iPTMnetiQ8R4I7.
PhosphoSiteiQ8R4I7.

Expressioni

Tissue specificityi

Expressed only in brain. Present throughout the central nervous system. Highly expressed in the hippocampal CA3 region, olfactory bulb and tubercle, caudate putamen, and neocortex in the adult brain.3 Publications

Developmental stagei

Observed restrictively in brain throughout embryonic and postnatal stages. Expression pattern in brain slightly changes from E13 to postnatal day 21 (P21). Expressed in both cerebrum and cerebellum throughout P0 to P35. In the cerebrum expression reached a plateau at P14 while expression in the cerebellum remains constant throughout all postnatal stages.2 Publications

Gene expression databases

BgeeiENSMUSG00000050321.
CleanExiMM_NETO1.
GenevisibleiQ8R4I7. MM.

Interactioni

Subunit structurei

Interacts with PLZ domains of DLG2, DLG3 and DLG4 via its C-terminal TRV domain. Interacts with GRIN2A and GRIN2B via its CUB domains.1 Publication

GO - Molecular functioni

  • ionotropic glutamate receptor binding Source: MGI

Protein-protein interaction databases

DIPiDIP-48395N.
IntActiQ8R4I7. 1 interaction.
STRINGi10090.ENSMUSP00000057340.

Structurei

3D structure databases

ProteinModelPortaliQ8R4I7.
SMRiQ8R4I7. Positions 41-269.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 155115CUB 1PROSITE-ProRule annotationAdd
BLAST
Domaini172 – 287116CUB 2PROSITE-ProRule annotationAdd
BLAST
Domaini291 – 32737LDL-receptor class APROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi531 – 5333PDZ-binding

Sequence similaritiesi

Contains 2 CUB domains.PROSITE-ProRule annotation
Contains 1 LDL-receptor class A domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJXA. Eukaryota.
ENOG410ZS19. LUCA.
GeneTreeiENSGT00760000119018.
HOGENOMiHOG000060303.
HOVERGENiHBG052600.
InParanoidiQ8R4I7.
OMAiTEAVDCK.
OrthoDBiEOG091G060O.
PhylomeDBiQ8R4I7.

Family and domain databases

Gene3Di2.60.120.290. 2 hits.
4.10.400.10. 1 hit.
InterProiIPR000859. CUB_dom.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR028867. NETO1.
[Graphical view]
PANTHERiPTHR10127:SF315. PTHR10127:SF315. 1 hit.
PfamiPF00431. CUB. 2 hits.
PF00057. Ldl_recept_a. 1 hit.
[Graphical view]
SMARTiSM00042. CUB. 2 hits.
SM00192. LDLa. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 2 hits.
SSF57424. SSF57424. 1 hit.
PROSITEiPS01180. CUB. 2 hits.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R4I7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIYGRSLFHI IASLIILHSS GATKKGTEKQ ITPETQKSVQ CGTWTKHAEG
60 70 80 90 100
GVFTSPNYPS KYPPDRECVY IIEAAPRQCI ELYFDEKYSI EPSWECKFDH
110 120 130 140 150
IEVRDGPFGF SPIIGRFCGQ QNPPVIKSSG RFLWIKFFAD GELESMGFSA
160 170 180 190 200
RYNFTPDPDF KDLGVLKPLP ACEFEMGGPE GIVESIQILK EGKASASEAV
210 220 230 240 250
DCKWYIRAPP RSKIYLRFLD YEMQNSNECK RNFVAVYDGS SSVEDLKAKF
260 270 280 290 300
CSTVANDVML RTGLGVIRMW ADEGSRNSRF QMLFTSFQEP PCEGNTFFCH
310 320 330 340 350
SNMCINNTLV CNGLQNCVYP WDENHCKEKR KTSLLDQLTN TSGTVIGVTS
360 370 380 390 400
CIVIILIIVS VIVQIKQPRK KYVQRKSDFD QTVFQEVFEP PHYELCTLRG
410 420 430 440 450
TGATADFADV AEDFENYHKL RRSSSKCIHD HHCGSQLSSA KGSRSNLSTR
460 470 480 490 500
DASILAEIPT QPVKPLIPPV NRRNILVMKH NYSQDAADAC DIDEIEEVPT
510 520 530
TSHRLSRHEK SVQRFCLIGS LSKHESEYNT TRV
Length:533
Mass (Da):60,242
Last modified:February 15, 2005 - v2
Checksum:i6375B395421D558F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti80 – 801I → F in BAC38196 (PubMed:16141072).Curated
Sequence conflicti514 – 5141R → Q in AAM18028 (PubMed:11943477).Curated
Sequence conflicti514 – 5141R → Q in BAC38196 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448840 mRNA. Translation: AAM18028.1.
AY138990 mRNA. Translation: AAN38318.1.
AK032510 mRNA. Translation: BAC27902.1.
AK081325 mRNA. Translation: BAC38196.1.
BC051145 mRNA. Translation: AAH51145.1.
CCDSiCCDS29387.1.
RefSeqiNP_659195.3. NM_144946.4.
XP_006526543.1. XM_006526480.2.
XP_006526544.1. XM_006526481.2.
XP_006526545.1. XM_006526482.2.
UniGeneiMm.329578.

Genome annotation databases

EnsembliENSMUST00000058829; ENSMUSP00000057340; ENSMUSG00000050321.
GeneIDi246317.
KEGGimmu:246317.
UCSCiuc008fuz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448840 mRNA. Translation: AAM18028.1.
AY138990 mRNA. Translation: AAN38318.1.
AK032510 mRNA. Translation: BAC27902.1.
AK081325 mRNA. Translation: BAC38196.1.
BC051145 mRNA. Translation: AAH51145.1.
CCDSiCCDS29387.1.
RefSeqiNP_659195.3. NM_144946.4.
XP_006526543.1. XM_006526480.2.
XP_006526544.1. XM_006526481.2.
XP_006526545.1. XM_006526482.2.
UniGeneiMm.329578.

3D structure databases

ProteinModelPortaliQ8R4I7.
SMRiQ8R4I7. Positions 41-269.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48395N.
IntActiQ8R4I7. 1 interaction.
STRINGi10090.ENSMUSP00000057340.

PTM databases

iPTMnetiQ8R4I7.
PhosphoSiteiQ8R4I7.

Proteomic databases

MaxQBiQ8R4I7.
PaxDbiQ8R4I7.
PeptideAtlasiQ8R4I7.
PRIDEiQ8R4I7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000058829; ENSMUSP00000057340; ENSMUSG00000050321.
GeneIDi246317.
KEGGimmu:246317.
UCSCiuc008fuz.2. mouse.

Organism-specific databases

CTDi81832.
MGIiMGI:2180216. Neto1.

Phylogenomic databases

eggNOGiENOG410IJXA. Eukaryota.
ENOG410ZS19. LUCA.
GeneTreeiENSGT00760000119018.
HOGENOMiHOG000060303.
HOVERGENiHBG052600.
InParanoidiQ8R4I7.
OMAiTEAVDCK.
OrthoDBiEOG091G060O.
PhylomeDBiQ8R4I7.

Miscellaneous databases

ChiTaRSiNeto1. mouse.
PROiQ8R4I7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000050321.
CleanExiMM_NETO1.
GenevisibleiQ8R4I7. MM.

Family and domain databases

Gene3Di2.60.120.290. 2 hits.
4.10.400.10. 1 hit.
InterProiIPR000859. CUB_dom.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR028867. NETO1.
[Graphical view]
PANTHERiPTHR10127:SF315. PTHR10127:SF315. 1 hit.
PfamiPF00431. CUB. 2 hits.
PF00057. Ldl_recept_a. 1 hit.
[Graphical view]
SMARTiSM00042. CUB. 2 hits.
SM00192. LDLa. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 2 hits.
SSF57424. SSF57424. 1 hit.
PROSITEiPS01180. CUB. 2 hits.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNETO1_MOUSE
AccessioniPrimary (citable) accession number: Q8R4I7
Secondary accession number(s): Q80X39, Q8C4S3, Q8CCM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: February 15, 2005
Last modified: September 7, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.