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Protein

Rho guanine nucleotide exchange factor 12

Gene

Arhgef12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGTPase activation, Guanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiR-MMU-193648 NRAGE signals death through JNK
R-MMU-194840 Rho GTPase cycle
R-MMU-416482 G alpha (12/13) signalling events
R-MMU-416572 Sema4D induced cell migration and growth-cone collapse

Names & Taxonomyi

Protein namesi
Recommended name:
Rho guanine nucleotide exchange factor 12
Alternative name(s):
Leukemia-associated RhoGEF
Gene namesi
Name:Arhgef12
Synonyms:Kiaa0382, Larg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1916882 Arhgef12

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000809312 – 1543Rho guanine nucleotide exchange factor 12Add BLAST1542

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei41PhosphoserineBy similarity1
Modified residuei309PhosphoserineCombined sources1
Modified residuei341PhosphoserineBy similarity1
Modified residuei637PhosphoserineBy similarity1
Modified residuei736PhosphothreonineBy similarity1
Modified residuei1288PhosphoserineBy similarity1
Modified residuei1327PhosphoserineCombined sources1
Modified residuei1377PhosphoserineBy similarity1
Modified residuei1457PhosphoserineBy similarity1
Modified residuei1540PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8R4H2
MaxQBiQ8R4H2
PaxDbiQ8R4H2
PeptideAtlasiQ8R4H2
PRIDEiQ8R4H2

PTM databases

iPTMnetiQ8R4H2
PhosphoSitePlusiQ8R4H2

Expressioni

Tissue specificityi

Expressed in brain, predominantly in neuronal cell bodies.1 Publication

Gene expression databases

BgeeiENSMUSG00000059495
CleanExiMM_ARHGEF12
ExpressionAtlasiQ8R4H2 baseline and differential
GenevisibleiQ8R4H2 MM

Interactioni

Subunit structurei

Interacts with GNA12 and GNA13, probably through the RGS-like domain, with RHOA, PLXNB1 and PLXNB2, and through its PDZ domain with IGF1R beta subunit. Interacts with GCSAM.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Plxnb1Q8CJH32EBI-8046267,EBI-2637650

GO - Molecular functioni

Protein-protein interaction databases

BioGridi213578, 2 interactors
IntActiQ8R4H2, 6 interactors
MINTiQ8R4H2
STRINGi10090.ENSMUSP00000126598

Structurei

3D structure databases

ProteinModelPortaliQ8R4H2
SMRiQ8R4H2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 151PDZPROSITE-ProRule annotationAdd BLAST80
Domaini367 – 558RGSLAdd BLAST192
Domaini787 – 977DHPROSITE-ProRule annotationAdd BLAST191
Domaini1019 – 1132PHPROSITE-ProRule annotationAdd BLAST114

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili194 – 262Sequence analysisAdd BLAST69
Coiled coili981 – 1004Sequence analysisAdd BLAST24

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3520 Eukaryota
COG5422 LUCA
GeneTreeiENSGT00760000119193
HOGENOMiHOG000034046
HOVERGENiHBG050571
InParanoidiQ8R4H2

Family and domain databases

CDDicd13390 PH_LARG, 1 hit
cd08754 RGS_LARG, 1 hit
cd00160 RhoGEF, 1 hit
Gene3Di1.20.900.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR037801 ARHGEF12_PH
IPR035899 DBL_dom_sf
IPR000219 DH-domain
IPR001331 GDS_CDC24_CS
IPR037884 LARG_RGS
IPR001478 PDZ
IPR036034 PDZ_sf
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR015212 RGS-like_dom
IPR036305 RGS_sf
PfamiView protein in Pfam
PF00595 PDZ, 1 hit
PF09128 RGS-like, 1 hit
PF00621 RhoGEF, 1 hit
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SM00233 PH, 1 hit
SM00325 RhoGEF, 1 hit
SUPFAMiSSF48065 SSF48065, 1 hit
SSF48097 SSF48097, 1 hit
SSF50156 SSF50156, 1 hit
PROSITEiView protein in PROSITE
PS00741 DH_1, 1 hit
PS50010 DH_2, 1 hit
PS50106 PDZ, 1 hit
PS50003 PH_DOMAIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R4H2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGTQSTITD RFPLKKPIRH GSILNRESPT DKKQKVERSS SHDFDPTDSS
60 70 80 90 100
SKKTKSSSEE SRSEIYGLVQ RCVIIQKDDN GFGLTVSGDN PVFVQSVKED
110 120 130 140 150
GAAMRAGVQT GDRIIKVNGT LVTHSNHLEV VKLIRSGSYV ALTVQGRPPG
160 170 180 190 200
SPQIPLADSE VEPSVTGHMS PIMTSPHSPG AAGNMERITS PVLVGEENNV
210 220 230 240 250
VHNQKVEILR KMLQKEQERL QLLQEDYNRT ATQRLLKEIQ EAKKHIPQLQ
260 270 280 290 300
EQLSKATGSA QDGAVIAPSR PLGDALTLSE AEADPGDGLC RTDWSSGDAS
310 320 330 340 350
RPSSDSADSP KSSLRERSYL EEAPERSEGV QDAEPQSLVG SPSTRGAPHI
360 370 380 390 400
IGAEDDDFGT EHEQINGQCS CFQSIELLKS RPAHLAVFLH HVVSQFDPAT
410 420 430 440 450
LLCYLYSDLY KQTNSKETRR VFLEFHQFFL DRSAHLKVPV PEEISVDLEK
460 470 480 490 500
RRPELIPEDL HRLYIQTMQE RVHPEVQRHL EDFRQKRSMG LTLAESELTK
510 520 530 540 550
LDAERDKDRG TLEKERACAE QIVTKIEEVL MTAQAVEEER SSTMQYVILM
560 570 580 590 600
YMKYLGVKVK EPRNLEHKRG RIGFLPKIKQ SMKKDREGEE KGKRRGFPSI
610 620 630 640 650
LGPPRRPSRH DNSAIGRAME IQKSRHPKHL STPSSVSPEP QDPAKLRQSG
660 670 680 690 700
VANEGTDTGY LPASSMSSAT SGTALSQEGG RENDTGTKQV GEASAPGDCL
710 720 730 740 750
DSTPRVPTTV FDFPPPLLDQ VQEEECEVER VAEHGTPKPF RKFDSIAFGE
760 770 780 790 800
SQSEDEQFEN DLETDPPNWQ QLVSREVLLG LKPSEIKRQE VINELFYTER
810 820 830 840 850
AHVRTLKVLD QVFYQRVSRE GILSPSELRK IFSNLEDILQ LHVGLNEQMK
860 870 880 890 900
AVRKRNETSV IDHIGEDLLI WFSGPGEEKL KHAAATFCSN QPFALEMIKS
910 920 930 940 950
RQKKDSRFHT FVQDAESNPL CRRLQLKDII PTQMQRLTKY PLLLDNIAKY
960 970 980 990 1000
TEWPPEREKV KKAADHCRQI LNYVNQAVRE AENKQRLEDY QRRLDTSNLK
1010 1020 1030 1040 1050
LSEYPNVDEL RNLDLTKRKM IHEGPLVWKV NRDKSIDLYT LLLEDILVLL
1060 1070 1080 1090 1100
QKQDDRLVLR CHSKILASTA DSKHTFSPVI KLSTVLVRQV ATDNKALFVI
1110 1120 1130 1140 1150
SMSDNGAQIY ELVAQTVSEK TVWQDLICRM AASVKEQSTK PIPLPQPPPC
1160 1170 1180 1190 1200
EGDNDEEEPA KLKVEHHDLS VAGLQSPDRV LGLESPLISS KPQSHSLNTP
1210 1220 1230 1240 1250
GKSAAEHLFV TATQFAKEQH ANGALKEGDG GYPVTIPGPH LPVSEERWAL
1260 1270 1280 1290 1300
DALRNLGLLK QLLVQQLGLT EKSTQEDWQS FSRYGPASEE VQADSGIRDL
1310 1320 1330 1340 1350
ENVKACHARE GQMSFKTGTG DIATCDSPRT STESCAAQDS VILASQDSQA
1360 1370 1380 1390 1400
SNVLVMDHMI LTPEMPPAEP EGGLDESGEH FFDAREAHSD DNPSEGDGAV
1410 1420 1430 1440 1450
KKEEKDVNLR ISGNCLILDG YDAVQESSTD EEVASSFPLQ PVTGIPAVDS
1460 1470 1480 1490 1500
SHQQQHSPQN VHPEGPVSPF TPEFLVQRHW RAMEDTCFEI QSPSCTDSQS
1510 1520 1530 1540
QILEYIHKIE ADLEHLKKVE ESYALLCQRL AGSALPDKLS DKS
Length:1,543
Mass (Da):172,349
Last modified:July 27, 2011 - v2
Checksum:i9A3D0B3BB8E40A86
GO

Sequence cautioni

The sequence BAC65549 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti307A → V in BAC65549 (PubMed:12693553).Curated1
Sequence conflicti320L → S in AAL87100 (Ref. 1) Curated1
Sequence conflicti334E → QE in BAC65549 (PubMed:12693553).Curated1
Sequence conflicti1447A → T in BAC65549 (PubMed:12693553).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF467766 mRNA Translation: AAL87100.1
AK122267 mRNA Translation: BAC65549.1 Different initiation.
AC110169 Genomic DNA No translation available.
AC160123 Genomic DNA No translation available.
RefSeqiXP_006510649.1, XM_006510586.3
UniGeneiMm.275266

Genome annotation databases

EnsembliENSMUST00000072767; ENSMUSP00000072547; ENSMUSG00000059495
GeneIDi69632
UCSCiuc009pbb.1 mouse

Similar proteinsi

Entry informationi

Entry nameiARHGC_MOUSE
AccessioniPrimary (citable) accession number: Q8R4H2
Secondary accession number(s): E9QPW9, Q80U18
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: July 27, 2011
Last modified: March 28, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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