Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Neuronal acetylcholine receptor subunit alpha-3

Gene

Chrna3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.By similarity

GO - Molecular functioni

  • acetylcholine-activated cation-selective channel activity Source: MGI
  • acetylcholine receptor activity Source: MGI

GO - Biological processi

  • activation of transmembrane receptor protein tyrosine kinase activity Source: MGI
  • behavioral response to nicotine Source: MGI
  • excitatory postsynaptic potential Source: MGI
  • locomotory behavior Source: MGI
  • nervous system development Source: MGI
  • regulation of acetylcholine secretion, neurotransmission Source: MGI
  • regulation of dendrite morphogenesis Source: MGI
  • regulation of membrane potential Source: MGI
  • regulation of muscle contraction Source: MGI
  • regulation of smooth muscle contraction Source: MGI
  • signal transduction Source: MGI
  • synaptic transmission, cholinergic Source: MGI
  • synaptic transmission involved in micturition Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit alpha-3
Gene namesi
Name:Chrna3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:87887. Chrna3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini26 – 234209ExtracellularSequence analysisAdd
BLAST
Transmembranei235 – 25925HelicalSequence analysisAdd
BLAST
Transmembranei267 – 28519HelicalSequence analysisAdd
BLAST
Transmembranei301 – 32222HelicalSequence analysisAdd
BLAST
Topological domaini323 – 471149CytoplasmicSequence analysisAdd
BLAST
Transmembranei472 – 49120HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • acetylcholine-gated channel complex Source: MGI
  • cell junction Source: UniProtKB-KW
  • postsynaptic membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3350222.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 499474Neuronal acetylcholine receptor subunit alpha-3PRO_0000000347Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi49 – 491N-linked (GlcNAc...)Sequence analysis
Disulfide bondi153 ↔ 167By similarity
Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence analysis
Disulfide bondi217 ↔ 218Associated with receptor activationBy similarity
Modified residuei407 – 4071PhosphoserineBy similarity
Modified residuei410 – 4101PhosphoserineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ8R4G9.
PaxDbiQ8R4G9.
PRIDEiQ8R4G9.

PTM databases

PhosphoSiteiQ8R4G9.

Expressioni

Gene expression databases

BgeeiQ8R4G9.

Interactioni

Subunit structurei

Neuronal AChR is composed of two different types of subunits: alpha and beta. Alpha-3 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors. Interacts with RIC3; which is required for proper folding and assembly (By similarity).By similarity

Protein-protein interaction databases

BioGridi225944. 2 interactions.
STRINGi10090.ENSMUSP00000034851.

Structurei

3D structure databases

ProteinModelPortaliQ8R4G9.
SMRiQ8R4G9. Positions 26-339, 425-495.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
HOVERGENiHBG003756.
InParanoidiQ8R4G9.
KOiK04805.
PhylomeDBiQ8R4G9.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R4G9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVVLPPPPL SMLMLVLMLL PVASASEAEH RLFQYLFEDY NEIIRPVANV
60 70 80 90 100
SHPVIIQFEV SMSQLVKVDE VNQIMETNLW LKQIWNDYKL KWKPSDYQGV
110 120 130 140 150
EFMRVPAEKI WKPDIVLYNN ADGDFQVDDK TKALLKYTGE VTWIPPAIFK
160 170 180 190 200
SSCKIDVTYF PFDYQNCTMK FGSWSYDKAK IDLVLIGSSM NLKDYWESGE
210 220 230 240 250
WAIIKAPGYK HEIKYNCCEE IYQDITYSLY IRRLPLFYTI NLIIPCLLIS
260 270 280 290 300
FLTVLVFYLP SDCGEKVTLC ISVLLSLTVF LLVITETIPS TSLVIPLIGE
310 320 330 340 350
YLLFTMIFVT LSIVITVFVL NVHYRTPTTH TMPTWVKAVF LNLLPRVMFM
360 370 380 390 400
TRPTSTEEDA PKTRNFYGAE LSNLNCFSRA DSKSCKEGYP CQDGTCGYCH
410 420 430 440 450
HRRVKISNFS ANLTRSSSSE SVDAVLSLSA LSPEIKEAIQ SVKYIAENMK
460 470 480 490
AQNVAKEIQD DWKYVAMVID RIFLWVFILV CILGTAGLFL QPLMARDDT
Length:499
Mass (Da):57,110
Last modified:June 1, 2002 - v1
Checksum:i721650E3F38D00FD
GO

Sequence cautioni

The sequence BAC37909.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF472588 mRNA. Translation: AAL84757.1.
AY574262 mRNA. Translation: AAS90358.1.
AK051730 mRNA. Translation: BAC34740.1.
AK053497 mRNA. Translation: BAC35404.1.
AK080415 mRNA. Translation: BAC37909.1. Different initiation.
RefSeqiNP_660111.2. NM_145129.2.
UniGeneiMm.63569.

Genome annotation databases

GeneIDi110834.
KEGGimmu:110834.
UCSCiuc009pry.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF472588 mRNA. Translation: AAL84757.1.
AY574262 mRNA. Translation: AAS90358.1.
AK051730 mRNA. Translation: BAC34740.1.
AK053497 mRNA. Translation: BAC35404.1.
AK080415 mRNA. Translation: BAC37909.1. Different initiation.
RefSeqiNP_660111.2. NM_145129.2.
UniGeneiMm.63569.

3D structure databases

ProteinModelPortaliQ8R4G9.
SMRiQ8R4G9. Positions 26-339, 425-495.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi225944. 2 interactions.
STRINGi10090.ENSMUSP00000034851.

Chemistry

ChEMBLiCHEMBL3350222.

PTM databases

PhosphoSiteiQ8R4G9.

Proteomic databases

MaxQBiQ8R4G9.
PaxDbiQ8R4G9.
PRIDEiQ8R4G9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi110834.
KEGGimmu:110834.
UCSCiuc009pry.1. mouse.

Organism-specific databases

CTDi1136.
MGIiMGI:87887. Chrna3.

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
HOVERGENiHBG003756.
InParanoidiQ8R4G9.
KOiK04805.
PhylomeDBiQ8R4G9.

Miscellaneous databases

ChiTaRSiChrna3. mouse.
PROiQ8R4G9.
SOURCEiSearch...

Gene expression databases

BgeeiQ8R4G9.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of mouse nicotinic acetylcholine receptor alpha 3 subunit cDNA."
    Lautner M.A., Stitzel J.A.
    Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: DBA/2Ibg.
    Tissue: Adrenal gland.
  2. Groot Kormelink P.J.
    Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cerebellum, Eye and Spinal ganglion.

Entry informationi

Entry nameiACHA3_MOUSE
AccessioniPrimary (citable) accession number: Q8R4G9
Secondary accession number(s): Q8BV44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: June 1, 2002
Last modified: July 6, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.