Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A - Mus musculus (Mouse)

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Q8R4G6 (MGT5A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 72. History...

Clusters with 100%, 90%, 50% identity |

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
EC=2.4.1.155

Alternative name(s):
Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase
GlcNAc-T V
Short name=GNT-V
Mannoside acetylglucosaminyltransferase 5
N-acetylglucosaminyl-transferase V

Gene names

Name:

Mgat5

Organism

Mus musculus (Mouse)

Taxonomic identifier

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	740 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 6-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Sequence similarities	Belongs to the glycosyltransferase 18 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein N-linked glycosylation Inferred from direct assay. Source: MGI
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

740

Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A

PRO_0000080523

Regions

Topological domain

13

Cytoplasmic Potential

Transmembrane

17

Helical; Signal-anchor for type II membrane protein; Potential

Topological domain

710

Lumenal Potential

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q8R4G6 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: E4D0A58BD6954C7A
Show »

740

84,551

        10         20         30         40         50         60 
MAFFSPWKLS SQKLGFFLVT FGFIWGMMLL HFTIQQRTQP ESSSMLREQI LDLSKRYIKA 

        70         80         90        100        110        120 
LAEENRDVVD GPYAGVMTAY DLKKTLAVLL DNILQRIGKL ESKVDNLVNG TGANSTNSTT 

       130        140        150        160        170        180 
AVPSLVSLEK INVADIINGV QEKCVLPPMD GYPHCEGKIK WMKDMWRSDP CYADYGVDGT 

       190        200        210        220        230        240 
SCSFFIYLSE VENWCPRLPW RAKNPYEEAD HNSLAEIRTD FNILYGMMKK HEEFRWMRLR 

       250        260        270        280        290        300 
IRRMADAWIQ AIKSLAEKQN LEKRKRKKIL VHLGLLTKES GFKIAETAFS GGPLGELVQW 

       310        320        330        340        350        360 
SDLITSLYLL GHDIRISASL AELKEIMKKV VGNRSGCPTV GDRIVELIYI DIVGLAQFKK 

       370        380        390        400        410        420 
TLGPSWVHYQ CMLRVLDSFG TEPEFNHASY AQSKGHKTPW GKWNLNPQQF YTMFPHTPDN 

       430        440        450        460        470        480 
SFLGFVVEQH LNSSDIHHIN EIKRQNQSLV YGKVDSFWKN KKIYLDIIHT YMEVHATVYG 

       490        500        510        520        530        540 
SSTKNIPSYV KNHGILSGRD LQFLLRETKL FVGLGFPYEG PAPLEAIANG CAFLNPKFNP 

       550        560        570        580        590        600 
PKSSKNTDFF IGKPTLRELT SQHPYAEVFI GRPHVWTVDL NNREEVEDAV KAILNQKIEP 

       610        620        630        640        650        660 
YMPYEFTCEG MLQRINAFIE KQDFCHGQVM WPPLSALQVK LAEPGQSCKQ VCQESQLICE 

       670        680        690        700        710        720 
PSFFQHLNKE KDLLKYKVTC QSSELYKDIL VPSFYPKSKH CVFQGDLLLF SCAGAHPTHQ 

       730        740 
RICPCRDFIK GQVALCKDCL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequences of the mouse N-acetylglucosaminyltransferase V (Mgat5) mRNA and an mRNA expressed by an Mgat5-deficient cell line." Alverez K., Haswell C., St Clair M., Perng G.S., Shorebah M., Pierce M., Fregien N. Glycobiology 12:389-394(2002) [PubMed: 12122020] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Pituitary.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF474154 mRNA. Translation: AAL83249.1. AK030402 mRNA. Translation: BAC26946.1.
IPI	IPI00322019.
RefSeq	NP_660110.2. NM_145128.3.
UniGene	Mm.214682. Mm.391592. Mm.472488.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8R4G6.
Protein family/group databases
CAZy	GT18. Glycosyltransferase Family 18.
PTM databases
PhosphoSite	Q8R4G6.
Proteomic databases
PRIDE	Q8R4G6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000038361; ENSMUSP00000038359; ENSMUSG00000036155. ENSMUST00000171405; ENSMUSP00000129166; ENSMUSG00000036155.
GeneID	107895.
KEGG	mmu:107895.
Organism-specific databases
CTD	4249.
MGI	MGI:894701. Mgat5.
Phylogenomic databases
GeneTree	ENSGT00390000012263.
HOGENOM	HBG505889.
HOVERGEN	HBG052469.
InParanoid	Q8R4G6.
OMA	ENWCPRL.
OrthoDB	EOG4HT8RM.
PhylomeDB	Q8R4G6.
Gene expression databases
ArrayExpress	Q8R4G6.
Bgee	Q8R4G6.
CleanEx	MM_MGAT5.
Genevestigator	Q8R4G6.
Family and domain databases
InterPro	IPR024144. Glyco_trans_18. [Graphical view]
KO	K00744.
PANTHER	PTHR15075. PTHR15075. 1 hit.
ProtoNet	Search...
Other
NextBio	359663.
SOURCE	Search...

Entry information

Entry name

MGT5A_MOUSE

Accession

Primary (citable) accession number: Q8R4G6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 1, 2002
Last modified:	November 16, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents