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Protein

Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A

Gene

Mgat5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides (By similarity).By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 6-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • acetylglucosaminyltransferase activity Source: MGI
  • alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity Source: MGI
  • transferase activity, transferring glycosyl groups Source: MGI

GO - Biological processi

  • protein N-linked glycosylation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-975577. N-Glycan antennae elongation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT18. Glycosyltransferase Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (EC:2.4.1.155)
Alternative name(s):
Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase
GlcNAc-T V
Short name:
GNT-V
Mannoside acetylglucosaminyltransferase 5
N-acetylglucosaminyl-transferase V
Gene namesi
Name:Mgat5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:894701. Mgat5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 13CytoplasmicSequence analysisAdd BLAST13
Transmembranei14 – 30Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
Topological domaini31 – 740LumenalSequence analysisAdd BLAST710

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • Golgi apparatus Source: MGI
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805231 – 740Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase AAdd BLAST740

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi109N-linked (GlcNAc...)Sequence analysis1
Glycosylationi114N-linked (GlcNAc...)Sequence analysis1
Glycosylationi117N-linked (GlcNAc...)Sequence analysis1
Glycosylationi333N-linked (GlcNAc...)Sequence analysis1
Glycosylationi432N-linked (GlcNAc...)Sequence analysis1
Glycosylationi446N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8R4G6.
PaxDbiQ8R4G6.
PRIDEiQ8R4G6.

PTM databases

iPTMnetiQ8R4G6.
PhosphoSitePlusiQ8R4G6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000036155.
CleanExiMM_MGAT5.
ExpressionAtlasiQ8R4G6. baseline and differential.
GenevisibleiQ8R4G6. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038359.

Structurei

3D structure databases

ProteinModelPortaliQ8R4G6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 18 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IDYS. Eukaryota.
ENOG410XTV7. LUCA.
GeneTreeiENSGT00390000012263.
HOGENOMiHOG000006557.
HOVERGENiHBG052469.
InParanoidiQ8R4G6.
KOiK00744.
OMAiGKVDNFW.
OrthoDBiEOG091G044F.
PhylomeDBiQ8R4G6.
TreeFamiTF313714.

Family and domain databases

InterProiIPR027833. DUF4525.
IPR026116. GlyclTrfase_18.
[Graphical view]
PANTHERiPTHR15075. PTHR15075. 1 hit.
PfamiPF15027. DUF4525. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R4G6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFFSPWKLS SQKLGFFLVT FGFIWGMMLL HFTIQQRTQP ESSSMLREQI
60 70 80 90 100
LDLSKRYIKA LAEENRDVVD GPYAGVMTAY DLKKTLAVLL DNILQRIGKL
110 120 130 140 150
ESKVDNLVNG TGANSTNSTT AVPSLVSLEK INVADIINGV QEKCVLPPMD
160 170 180 190 200
GYPHCEGKIK WMKDMWRSDP CYADYGVDGT SCSFFIYLSE VENWCPRLPW
210 220 230 240 250
RAKNPYEEAD HNSLAEIRTD FNILYGMMKK HEEFRWMRLR IRRMADAWIQ
260 270 280 290 300
AIKSLAEKQN LEKRKRKKIL VHLGLLTKES GFKIAETAFS GGPLGELVQW
310 320 330 340 350
SDLITSLYLL GHDIRISASL AELKEIMKKV VGNRSGCPTV GDRIVELIYI
360 370 380 390 400
DIVGLAQFKK TLGPSWVHYQ CMLRVLDSFG TEPEFNHASY AQSKGHKTPW
410 420 430 440 450
GKWNLNPQQF YTMFPHTPDN SFLGFVVEQH LNSSDIHHIN EIKRQNQSLV
460 470 480 490 500
YGKVDSFWKN KKIYLDIIHT YMEVHATVYG SSTKNIPSYV KNHGILSGRD
510 520 530 540 550
LQFLLRETKL FVGLGFPYEG PAPLEAIANG CAFLNPKFNP PKSSKNTDFF
560 570 580 590 600
IGKPTLRELT SQHPYAEVFI GRPHVWTVDL NNREEVEDAV KAILNQKIEP
610 620 630 640 650
YMPYEFTCEG MLQRINAFIE KQDFCHGQVM WPPLSALQVK LAEPGQSCKQ
660 670 680 690 700
VCQESQLICE PSFFQHLNKE KDLLKYKVTC QSSELYKDIL VPSFYPKSKH
710 720 730 740
CVFQGDLLLF SCAGAHPTHQ RICPCRDFIK GQVALCKDCL
Length:740
Mass (Da):84,551
Last modified:June 1, 2002 - v1
Checksum:iE4D0A58BD6954C7A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF474154 mRNA. Translation: AAL83249.1.
AK030402 mRNA. Translation: BAC26946.1.
CCDSiCCDS15245.1.
RefSeqiNP_660110.2. NM_145128.3.
XP_006529118.1. XM_006529055.3.
XP_006529119.1. XM_006529056.3.
XP_006529120.1. XM_006529057.3.
UniGeneiMm.214682.
Mm.391592.
Mm.472488.

Genome annotation databases

EnsembliENSMUST00000038361; ENSMUSP00000038359; ENSMUSG00000036155.
ENSMUST00000171405; ENSMUSP00000129166; ENSMUSG00000036155.
GeneIDi107895.
KEGGimmu:107895.
UCSCiuc007ckq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF474154 mRNA. Translation: AAL83249.1.
AK030402 mRNA. Translation: BAC26946.1.
CCDSiCCDS15245.1.
RefSeqiNP_660110.2. NM_145128.3.
XP_006529118.1. XM_006529055.3.
XP_006529119.1. XM_006529056.3.
XP_006529120.1. XM_006529057.3.
UniGeneiMm.214682.
Mm.391592.
Mm.472488.

3D structure databases

ProteinModelPortaliQ8R4G6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038359.

Protein family/group databases

CAZyiGT18. Glycosyltransferase Family 18.

PTM databases

iPTMnetiQ8R4G6.
PhosphoSitePlusiQ8R4G6.

Proteomic databases

MaxQBiQ8R4G6.
PaxDbiQ8R4G6.
PRIDEiQ8R4G6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038361; ENSMUSP00000038359; ENSMUSG00000036155.
ENSMUST00000171405; ENSMUSP00000129166; ENSMUSG00000036155.
GeneIDi107895.
KEGGimmu:107895.
UCSCiuc007ckq.1. mouse.

Organism-specific databases

CTDi4249.
MGIiMGI:894701. Mgat5.

Phylogenomic databases

eggNOGiENOG410IDYS. Eukaryota.
ENOG410XTV7. LUCA.
GeneTreeiENSGT00390000012263.
HOGENOMiHOG000006557.
HOVERGENiHBG052469.
InParanoidiQ8R4G6.
KOiK00744.
OMAiGKVDNFW.
OrthoDBiEOG091G044F.
PhylomeDBiQ8R4G6.
TreeFamiTF313714.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-MMU-975577. N-Glycan antennae elongation.

Miscellaneous databases

ChiTaRSiMgat5. mouse.
PROiQ8R4G6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036155.
CleanExiMM_MGAT5.
ExpressionAtlasiQ8R4G6. baseline and differential.
GenevisibleiQ8R4G6. MM.

Family and domain databases

InterProiIPR027833. DUF4525.
IPR026116. GlyclTrfase_18.
[Graphical view]
PANTHERiPTHR15075. PTHR15075. 1 hit.
PfamiPF15027. DUF4525. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMGT5A_MOUSE
AccessioniPrimary (citable) accession number: Q8R4G6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 1, 2002
Last modified: November 2, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.