Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A - Mus musculus (Mouse)

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Reviewed, UniProtKB/Swiss-Prot Q8R4G6 (MGT5A_MOUSE)

Last modified June 16, 2009. Version 49. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
    Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
    EC=2.4.1.155
Alternative name(s):
    Mannoside acetylglucosaminyltransferase 5
    Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase
    N-acetylglucosaminyl-transferase V
    GlcNAc-T V
      Short name=GNT-V

Gene names

Name:

Mgat5

Organism

Mus musculus (Mouse)

Taxonomic identifier

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	740 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 6-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Sequence similarities	Belongs to the glycosyltransferase 18 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	protein amino acid N-linked glycosylation Inferred from direct assay. Source: MGI
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

740

Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A

PRO_0000080523

Regions

Topological domain

13

Cytoplasmic Potential

Transmembrane

17

Signal-anchor for type II membrane protein Potential

Topological domain

710

Lumenal Potential

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q8R4G6-1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: E4D0A58BD6954C7A
Show »

740

84,551

        10         20         30         40         50         60 
MAFFSPWKLS SQKLGFFLVT FGFIWGMMLL HFTIQQRTQP ESSSMLREQI LDLSKRYIKA 

        70         80         90        100        110        120 
LAEENRDVVD GPYAGVMTAY DLKKTLAVLL DNILQRIGKL ESKVDNLVNG TGANSTNSTT 

       130        140        150        160        170        180 
AVPSLVSLEK INVADIINGV QEKCVLPPMD GYPHCEGKIK WMKDMWRSDP CYADYGVDGT 

       190        200        210        220        230        240 
SCSFFIYLSE VENWCPRLPW RAKNPYEEAD HNSLAEIRTD FNILYGMMKK HEEFRWMRLR 

       250        260        270        280        290        300 
IRRMADAWIQ AIKSLAEKQN LEKRKRKKIL VHLGLLTKES GFKIAETAFS GGPLGELVQW 

       310        320        330        340        350        360 
SDLITSLYLL GHDIRISASL AELKEIMKKV VGNRSGCPTV GDRIVELIYI DIVGLAQFKK 

       370        380        390        400        410        420 
TLGPSWVHYQ CMLRVLDSFG TEPEFNHASY AQSKGHKTPW GKWNLNPQQF YTMFPHTPDN 

       430        440        450        460        470        480 
SFLGFVVEQH LNSSDIHHIN EIKRQNQSLV YGKVDSFWKN KKIYLDIIHT YMEVHATVYG 

       490        500        510        520        530        540 
SSTKNIPSYV KNHGILSGRD LQFLLRETKL FVGLGFPYEG PAPLEAIANG CAFLNPKFNP 

       550        560        570        580        590        600 
PKSSKNTDFF IGKPTLRELT SQHPYAEVFI GRPHVWTVDL NNREEVEDAV KAILNQKIEP 

       610        620        630        640        650        660 
YMPYEFTCEG MLQRINAFIE KQDFCHGQVM WPPLSALQVK LAEPGQSCKQ VCQESQLICE 

       670        680        690        700        710        720 
PSFFQHLNKE KDLLKYKVTC QSSELYKDIL VPSFYPKSKH CVFQGDLLLF SCAGAHPTHQ 

       730        740 
RICPCRDFIK GQVALCKDCL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequences of the mouse N-acetylglucosaminyltransferase V (Mgat5) mRNA and an mRNA expressed by an Mgat5-deficient cell line." Alverez K., Haswell C., St Clair M., Perng G.S., Shorebah M., Pierce M., Fregien N. Glycobiology 12:389-394(2002) [PubMed: 12122020] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Pituitary.
+	Additional computationally mapped references.

Cross-references

Sequence databases
	AF474154 mRNA. Translation: AAL83249.1. AK030402 mRNA. Translation: BAC26946.1.
IPI	IPI00322019.
RefSeq	NP_660110.2.
UniGene	Mm.214682 Mm.391592 Mm.472488
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT18. Glycosyltransferase Family 18.
PTM databases
PhosphoSite	Q8R4G6.
Proteomic databases
PRIDE	Q8R4G6.
Genome annotation databases
Ensembl	ENSMUSG00000036155. Mus musculus. [Contig view]
GeneID	107895.
KEGG	mmu:107895.
Organism-specific databases
MGI	MGI:894701. Mgat5.
Phylogenomic databases
HOGENOM	Q8R4G6.
HOVERGEN	Q8R4G6.
OMA	Q8R4G6. ENQLICE.
Enzyme and pathway databases
BRENDA	2.4.1.155. 244.
Gene expression databases
ArrayExpress	Q8R4G6.
CleanEx	MM_MGAT5.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	359663.
SOURCE	Search...

Entry information

Entry name

MGT5A_MOUSE

Accession

Primary (citable) accession number: Q8R4G6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents