Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Netrin-G2

Gene

Ntng2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in controlling patterning and neuronal circuit formation at the laminar, cellular, subcellular and synaptic levels. Promotes neurite outgrowth of both axons and dendrites.1 Publication

GO - Biological processi

  • axonogenesis Source: MGI

Keywordsi

Molecular functionDevelopmental protein
Biological processDifferentiation, Neurogenesis

Enzyme and pathway databases

ReactomeiR-MMU-163125 Post-translational modification: synthesis of GPI-anchored proteins

Names & Taxonomyi

Protein namesi
Recommended name:
Netrin-G2
Alternative name(s):
Laminet-2
Gene namesi
Name:Ntng2
Synonyms:Lmnt2
OrganismiMus musculus (Mouse)Curated
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2159341 Ntng2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000001709718 – 566Netrin-G2Add BLAST549
PropeptideiPRO_0000017098567 – 589Removed in mature formSequence analysisAdd BLAST23

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi22 ↔ 39By similarity
Disulfide bondi61 ↔ 81By similarity
Disulfide bondi69 ↔ 77By similarity
Glycosylationi122N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi128N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi171 ↔ 195By similarity
Disulfide bondi287 ↔ 296By similarity
Disulfide bondi289 ↔ 305By similarity
Disulfide bondi307 ↔ 316By similarity
Glycosylationi310N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi319 ↔ 344By similarity
Disulfide bondi413 ↔ 422Sequence analysis
Disulfide bondi415 ↔ 433Sequence analysis
Disulfide bondi436 ↔ 445Sequence analysis
Disulfide bondi448 ↔ 466Sequence analysis
Glycosylationi455N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi469 ↔ 481Sequence analysis
Disulfide bondi471 ↔ 487Sequence analysis
Glycosylationi482N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi489 ↔ 498Sequence analysis
Disulfide bondi501 ↔ 511Sequence analysis
Disulfide bondi516 ↔ 529By similarity
Disulfide bondi523 ↔ 535By similarity
Disulfide bondi537 ↔ 546By similarity
Lipidationi566GPI-anchor amidated glycineSequence analysis1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ8R4F1
PRIDEiQ8R4F1

PTM databases

PhosphoSitePlusiQ8R4F1

Expressioni

Tissue specificityi

Expression is restricted primarily to neurons of the CNS, particularly in the cerebral cortex, habenular nucleus and superior colliculus. Low levels in lung, kidney, heart and spleen.2 Publications

Developmental stagei

Expression is detected from embryonic day 9. Strong expression is maintained from embryonic day 14 well into adulthood.1 Publication

Gene expression databases

BgeeiENSMUSG00000035513
CleanExiMM_NTNG2
ExpressionAtlasiQ8R4F1 baseline and differential
GenevisibleiQ8R4F1 MM

Interactioni

Subunit structurei

Interacts with LRRC4.2 Publications

Protein-protein interaction databases

IntActiQ8R4F1, 1 interactor
STRINGi10090.ENSMUSP00000035468

Structurei

3D structure databases

ProteinModelPortaliQ8R4F1
SMRiQ8R4F1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 286Laminin N-terminalPROSITE-ProRule annotationAdd BLAST252
Domaini287 – 346Laminin EGF-like 1PROSITE-ProRule annotationAdd BLAST60
Domaini413 – 468Laminin EGF-like 2PROSITE-ProRule annotationAdd BLAST56
Domaini469 – 513Laminin EGF-like 3PROSITE-ProRule annotationAdd BLAST45

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni69 – 88NGL discriminant loop IBy similarityAdd BLAST20
Regioni201 – 203NGL discriminant loop IIBy similarity3
Regioni264 – 267NGL discriminant loop IIIBy similarity4

Domaini

The laminin N-terminal domain mediates 1:1 binding to NGL ligand with sub-micromolar affinity. Three NGL-binding loops mediate discrimination for LRRC4/NGL2 among other NGLs by binding specifically to its LRR repeats. This specificity drives the sorting of a mixed population of molecules into discrete cell surface subdomains (By similarity).By similarity

Keywords - Domaini

Laminin EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG3512 Eukaryota
ENOG410XS7U LUCA
GeneTreeiENSGT00890000139348
HOGENOMiHOG000231614
HOVERGENiHBG052676
InParanoidiQ8R4F1
KOiK16359
OMAiQHCQHCR
OrthoDBiEOG091G05AV
TreeFamiTF333945

Family and domain databases

Gene3Di2.60.120.1490, 1 hit
InterProiView protein in InterPro
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR002049 Laminin_EGF
IPR008211 Laminin_N
IPR038684 Laminin_N_sf
IPR036278 Sialidase_sf
PfamiView protein in Pfam
PF00053 Laminin_EGF, 3 hits
PF00055 Laminin_N, 1 hit
SMARTiView protein in SMART
SM00181 EGF, 2 hits
SM00180 EGF_Lam, 3 hits
SM00136 LamNT, 1 hit
SUPFAMiSSF50939 SSF50939, 2 hits
PROSITEiView protein in PROSITE
PS00022 EGF_1, 3 hits
PS01186 EGF_2, 1 hit
PS50026 EGF_3, 1 hit
PS01248 EGF_LAM_1, 2 hits
PS50027 EGF_LAM_2, 3 hits
PS51117 LAMININ_NTER, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2B1 Publication (identifier: Q8R4F1-1) [UniParc]FASTAAdd to basket
Also known as: G2b1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLRLLALFLH CLPLVSGDYD ICKSWVTTDE GPTWEFYACQ PKVMRLKDYV
60 70 80 90 100
KVKVEPSGIT CGDPPERFCS HENPYLCSNE CDASNPDLAH PPRLMFDRED
110 120 130 140 150
EGLATYWQSV TWSRYPSPLE ANITLSWNKS VELTDDVVVT FEYGRPTVMV
160 170 180 190 200
LEKSLDNGRT WQPYQFYAED CMEAFGMSAR RARDMSPSSA HRVLCTEEYS
210 220 230 240 250
RWAGSKKEKH VRFEVRDRFA IFAGPDLRNM DNLYTRMESA KGLKEFFTFT
260 270 280 290 300
DLRMRLLRPA LGGTYVQREN LYKYFYAISN IEVIGRCKCN LHANLCTVRE
310 320 330 340 350
GSLQCECEHN TTGPDCGRCK KNFRTRAWRA GSYLPLPHGS PNACAAAGSA
360 370 380 390 400
FGSQTKPPTM APLGDSSFWP QVSSSAEAVA ISVAVPSQAK DSTLFELKPR
410 420 430 440 450
SPQVIPIEEF QDCECYGHSN RCSYIDFLNV VTCVSCKHNT RGQHCQHCRL
460 470 480 490 500
GYYRNGSAEL DDENVCIECN CNQIGSVHDR CNETGFCECR EGAVGPKCDD
510 520 530 540 550
CLPTHYWRQG CYPNVCDDDQ LLCQNGGTCQ QNQRCACPPG YTGIRCEQPR
560 570 580
CDLADDAGPD CDRAPGIVPR PDTLLGCLLL LGLAARLAC
Length:589
Mass (Da):66,166
Last modified:July 27, 2011 - v2
Checksum:iF2AB419F0EE074F7
GO
Isoform 2A1 Publication (identifier: Q8R4F1-2) [UniParc]FASTAAdd to basket
Also known as: G2a1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     353-411: Missing.
     412-412: D → N

Show »
Length:530
Mass (Da):59,869
Checksum:i1C9D908035342C64
GO
Isoform 2CCurated (identifier: Q8R4F1-3) [UniParc]FASTAAdd to basket
Also known as: G2c1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     353-377: Missing.
     378-378: A → T

Show »
Length:564
Mass (Da):63,578
Checksum:iF5239CC7ABFF196B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41P → S in AAL84788 (PubMed:11906208).Curated1
Sequence conflicti41P → S in AAL84789 (PubMed:11906208).Curated1
Sequence conflicti392S → F in AAL84789 (PubMed:11906208).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_050562353 – 411Missing in isoform 2A. 2 PublicationsAdd BLAST59
Alternative sequenceiVSP_050564353 – 377Missing in isoform 2C. 2 PublicationsAdd BLAST25
Alternative sequenceiVSP_050565378A → T in isoform 2C. 2 Publications1
Alternative sequenceiVSP_050563412D → N in isoform 2A. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB052336 mRNA Translation: BAB71994.1
AB052337 mRNA Translation: BAB71995.1
AB052338 mRNA Translation: BAB71996.1
AF475079 mRNA Translation: AAL84788.1
AF475080 mRNA Translation: AAL84789.1
AL772379 Genomic DNA Translation: CAM16243.1
CH466542 Genomic DNA Translation: EDL08409.1
CCDSiCCDS15851.1 [Q8R4F1-2]
CCDS15852.1 [Q8R4F1-1]
CCDS84499.1 [Q8R4F1-3]
RefSeqiNP_001292734.1, NM_001305805.1 [Q8R4F1-3]
NP_598007.1, NM_133500.2 [Q8R4F1-2]
NP_598008.1, NM_133501.2 [Q8R4F1-1]
UniGeneiMm.442448

Genome annotation databases

EnsembliENSMUST00000048455; ENSMUSP00000035468; ENSMUSG00000035513 [Q8R4F1-1]
ENSMUST00000091153; ENSMUSP00000088688; ENSMUSG00000035513 [Q8R4F1-3]
ENSMUST00000102873; ENSMUSP00000099937; ENSMUSG00000035513 [Q8R4F1-2]
GeneIDi171171
KEGGimmu:171171
UCSCiuc008izo.2 mouse [Q8R4F1-1]
uc012btf.2 mouse [Q8R4F1-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNTNG2_MOUSE
AccessioniPrimary (citable) accession number: Q8R4F1
Secondary accession number(s): A2AKX0
, Q8R4F2, Q8VIP6, Q8VIP7, Q8VIP8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: July 27, 2011
Last modified: April 25, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health