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Protein

Netrin-G2

Gene

Ntng2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in controlling patterning and neuronal circuit formation at the laminar, cellular, subcellular and synaptic levels. Promotes neurite outgrowth of both axons and dendrites.1 Publication

GO - Biological processi

  • axonogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Netrin-G2
Alternative name(s):
Laminet-2
Gene namesi
Name:Ntng2
Synonyms:Lmnt2
OrganismiMus musculus (Mouse)Curated
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2159341. Ntng2.

Subcellular locationi

  • Cell membrane 1 Publication; Lipid-anchorGPI-anchor 1 Publication; Extracellular side 1 Publication

GO - Cellular componenti

  • anchored component of plasma membrane Source: UniProtKB
  • axon Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence analysisAdd
BLAST
Chaini18 – 566549Netrin-G2PRO_0000017097Add
BLAST
Propeptidei567 – 58923Removed in mature formSequence analysisPRO_0000017098Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi22 ↔ 39By similarity
Disulfide bondi61 ↔ 81By similarity
Disulfide bondi69 ↔ 77By similarity
Glycosylationi122 – 1221N-linked (GlcNAc...)Sequence analysis
Glycosylationi128 – 1281N-linked (GlcNAc...)Sequence analysis
Disulfide bondi171 ↔ 195By similarity
Disulfide bondi287 ↔ 296By similarity
Disulfide bondi289 ↔ 305By similarity
Disulfide bondi307 ↔ 316By similarity
Glycosylationi310 – 3101N-linked (GlcNAc...)Sequence analysis
Disulfide bondi319 ↔ 344By similarity
Disulfide bondi413 ↔ 422Sequence analysis
Disulfide bondi415 ↔ 433Sequence analysis
Disulfide bondi436 ↔ 445Sequence analysis
Disulfide bondi448 ↔ 466Sequence analysis
Glycosylationi455 – 4551N-linked (GlcNAc...)Sequence analysis
Disulfide bondi469 ↔ 481Sequence analysis
Disulfide bondi471 ↔ 487Sequence analysis
Glycosylationi482 – 4821N-linked (GlcNAc...)Sequence analysis
Disulfide bondi489 ↔ 498Sequence analysis
Disulfide bondi501 ↔ 511Sequence analysis
Disulfide bondi516 ↔ 529By similarity
Disulfide bondi523 ↔ 535By similarity
Disulfide bondi537 ↔ 546By similarity
Lipidationi566 – 5661GPI-anchor amidated glycineSequence analysis

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiQ8R4F1.
PaxDbiQ8R4F1.
PRIDEiQ8R4F1.

PTM databases

PhosphoSiteiQ8R4F1.

Expressioni

Tissue specificityi

Expression is restricted primarily to neurons of the CNS, particularly in the cerebral cortex, habenular nucleus and superior colliculus. Low levels in lung, kidney, heart and spleen.2 Publications

Developmental stagei

Expression is detected from embryonic day 9. Strong expression is maintained from embryonic day 14 well into adulthood.1 Publication

Gene expression databases

BgeeiENSMUSG00000035513.
CleanExiMM_NTNG2.
ExpressionAtlasiQ8R4F1. baseline and differential.
GenevisibleiQ8R4F1. MM.

Interactioni

Subunit structurei

Interacts with LRRC4.2 Publications

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035468.

Structurei

3D structure databases

ProteinModelPortaliQ8R4F1.
SMRiQ8R4F1. Positions 18-348, 412-562.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 286252Laminin N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini287 – 34660Laminin EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini413 – 46856Laminin EGF-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini469 – 51345Laminin EGF-like 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni69 – 8820NGL discriminant loop IBy similarityAdd
BLAST
Regioni201 – 2033NGL discriminant loop IIBy similarity
Regioni264 – 2674NGL discriminant loop IIIBy similarity

Domaini

The laminin N-terminal domain mediates 1:1 binding to NGL ligand with sub-micromolar affinity. Three NGL-binding loops mediate discrimination for LRRC4/NGL2 among other NGLs by binding specifically to its LRR repeats. This specificity drives the sorting of a mixed population of molecules into discrete cell surface subdomains (By similarity).By similarity

Sequence similaritiesi

Contains 3 laminin EGF-like domains.PROSITE-ProRule annotationCurated
Contains 1 laminin N-terminal domain.PROSITE-ProRule annotationCurated

Keywords - Domaini

Laminin EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG3512. Eukaryota.
ENOG410XS7U. LUCA.
GeneTreeiENSGT00840000129717.
HOGENOMiHOG000231614.
HOVERGENiHBG052676.
InParanoidiQ8R4F1.
KOiK16359.
OMAiNCECYGH.
OrthoDBiEOG091G05AV.
TreeFamiTF333945.

Family and domain databases

InterProiIPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR002049. Laminin_EGF.
IPR008211. Laminin_N.
IPR011040. Sialidases.
[Graphical view]
PfamiPF00053. Laminin_EGF. 3 hits.
PF00055. Laminin_N. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 2 hits.
SM00180. EGF_Lam. 3 hits.
SM00136. LamNT. 1 hit.
[Graphical view]
SUPFAMiSSF50939. SSF50939. 2 hits.
PROSITEiPS00022. EGF_1. 3 hits.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS01248. EGF_LAM_1. 2 hits.
PS50027. EGF_LAM_2. 3 hits.
PS51117. LAMININ_NTER. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2B1 Publication (identifier: Q8R4F1-1) [UniParc]FASTAAdd to basket
Also known as: G2b1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLRLLALFLH CLPLVSGDYD ICKSWVTTDE GPTWEFYACQ PKVMRLKDYV
60 70 80 90 100
KVKVEPSGIT CGDPPERFCS HENPYLCSNE CDASNPDLAH PPRLMFDRED
110 120 130 140 150
EGLATYWQSV TWSRYPSPLE ANITLSWNKS VELTDDVVVT FEYGRPTVMV
160 170 180 190 200
LEKSLDNGRT WQPYQFYAED CMEAFGMSAR RARDMSPSSA HRVLCTEEYS
210 220 230 240 250
RWAGSKKEKH VRFEVRDRFA IFAGPDLRNM DNLYTRMESA KGLKEFFTFT
260 270 280 290 300
DLRMRLLRPA LGGTYVQREN LYKYFYAISN IEVIGRCKCN LHANLCTVRE
310 320 330 340 350
GSLQCECEHN TTGPDCGRCK KNFRTRAWRA GSYLPLPHGS PNACAAAGSA
360 370 380 390 400
FGSQTKPPTM APLGDSSFWP QVSSSAEAVA ISVAVPSQAK DSTLFELKPR
410 420 430 440 450
SPQVIPIEEF QDCECYGHSN RCSYIDFLNV VTCVSCKHNT RGQHCQHCRL
460 470 480 490 500
GYYRNGSAEL DDENVCIECN CNQIGSVHDR CNETGFCECR EGAVGPKCDD
510 520 530 540 550
CLPTHYWRQG CYPNVCDDDQ LLCQNGGTCQ QNQRCACPPG YTGIRCEQPR
560 570 580
CDLADDAGPD CDRAPGIVPR PDTLLGCLLL LGLAARLAC
Length:589
Mass (Da):66,166
Last modified:July 27, 2011 - v2
Checksum:iF2AB419F0EE074F7
GO
Isoform 2A1 Publication (identifier: Q8R4F1-2) [UniParc]FASTAAdd to basket
Also known as: G2a1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     353-411: Missing.
     412-412: D → N

Show »
Length:530
Mass (Da):59,869
Checksum:i1C9D908035342C64
GO
Isoform 2CCurated (identifier: Q8R4F1-3) [UniParc]FASTAAdd to basket
Also known as: G2c1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     353-377: Missing.
     378-378: A → T

Show »
Length:564
Mass (Da):63,578
Checksum:iF5239CC7ABFF196B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti41 – 411P → S in AAL84788 (PubMed:11906208).Curated
Sequence conflicti41 – 411P → S in AAL84789 (PubMed:11906208).Curated
Sequence conflicti392 – 3921S → F in AAL84789 (PubMed:11906208).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei353 – 41159Missing in isoform 2A. 2 PublicationsVSP_050562Add
BLAST
Alternative sequencei353 – 37725Missing in isoform 2C. 2 PublicationsVSP_050564Add
BLAST
Alternative sequencei378 – 3781A → T in isoform 2C. 2 PublicationsVSP_050565
Alternative sequencei412 – 4121D → N in isoform 2A. 2 PublicationsVSP_050563

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB052336 mRNA. Translation: BAB71994.1.
AB052337 mRNA. Translation: BAB71995.1.
AB052338 mRNA. Translation: BAB71996.1.
AF475079 mRNA. Translation: AAL84788.1.
AF475080 mRNA. Translation: AAL84789.1.
AL772379 Genomic DNA. Translation: CAM16243.1.
CH466542 Genomic DNA. Translation: EDL08409.1.
CCDSiCCDS15851.1. [Q8R4F1-2]
CCDS15852.1. [Q8R4F1-1]
RefSeqiNP_001292734.1. NM_001305805.1. [Q8R4F1-3]
NP_598007.1. NM_133500.2. [Q8R4F1-2]
NP_598008.1. NM_133501.2. [Q8R4F1-1]
UniGeneiMm.442448.

Genome annotation databases

EnsembliENSMUST00000048455; ENSMUSP00000035468; ENSMUSG00000035513. [Q8R4F1-1]
ENSMUST00000091153; ENSMUSP00000088688; ENSMUSG00000035513. [Q8R4F1-3]
ENSMUST00000102873; ENSMUSP00000099937; ENSMUSG00000035513. [Q8R4F1-2]
GeneIDi171171.
KEGGimmu:171171.
UCSCiuc008izo.2. mouse. [Q8R4F1-1]
uc012btf.2. mouse. [Q8R4F1-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB052336 mRNA. Translation: BAB71994.1.
AB052337 mRNA. Translation: BAB71995.1.
AB052338 mRNA. Translation: BAB71996.1.
AF475079 mRNA. Translation: AAL84788.1.
AF475080 mRNA. Translation: AAL84789.1.
AL772379 Genomic DNA. Translation: CAM16243.1.
CH466542 Genomic DNA. Translation: EDL08409.1.
CCDSiCCDS15851.1. [Q8R4F1-2]
CCDS15852.1. [Q8R4F1-1]
RefSeqiNP_001292734.1. NM_001305805.1. [Q8R4F1-3]
NP_598007.1. NM_133500.2. [Q8R4F1-2]
NP_598008.1. NM_133501.2. [Q8R4F1-1]
UniGeneiMm.442448.

3D structure databases

ProteinModelPortaliQ8R4F1.
SMRiQ8R4F1. Positions 18-348, 412-562.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035468.

PTM databases

PhosphoSiteiQ8R4F1.

Proteomic databases

MaxQBiQ8R4F1.
PaxDbiQ8R4F1.
PRIDEiQ8R4F1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048455; ENSMUSP00000035468; ENSMUSG00000035513. [Q8R4F1-1]
ENSMUST00000091153; ENSMUSP00000088688; ENSMUSG00000035513. [Q8R4F1-3]
ENSMUST00000102873; ENSMUSP00000099937; ENSMUSG00000035513. [Q8R4F1-2]
GeneIDi171171.
KEGGimmu:171171.
UCSCiuc008izo.2. mouse. [Q8R4F1-1]
uc012btf.2. mouse. [Q8R4F1-3]

Organism-specific databases

CTDi84628.
MGIiMGI:2159341. Ntng2.

Phylogenomic databases

eggNOGiKOG3512. Eukaryota.
ENOG410XS7U. LUCA.
GeneTreeiENSGT00840000129717.
HOGENOMiHOG000231614.
HOVERGENiHBG052676.
InParanoidiQ8R4F1.
KOiK16359.
OMAiNCECYGH.
OrthoDBiEOG091G05AV.
TreeFamiTF333945.

Miscellaneous databases

ChiTaRSiNtng2. mouse.
PROiQ8R4F1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035513.
CleanExiMM_NTNG2.
ExpressionAtlasiQ8R4F1. baseline and differential.
GenevisibleiQ8R4F1. MM.

Family and domain databases

InterProiIPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR002049. Laminin_EGF.
IPR008211. Laminin_N.
IPR011040. Sialidases.
[Graphical view]
PfamiPF00053. Laminin_EGF. 3 hits.
PF00055. Laminin_N. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 2 hits.
SM00180. EGF_Lam. 3 hits.
SM00136. LamNT. 1 hit.
[Graphical view]
SUPFAMiSSF50939. SSF50939. 2 hits.
PROSITEiPS00022. EGF_1. 3 hits.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS01248. EGF_LAM_1. 2 hits.
PS50027. EGF_LAM_2. 3 hits.
PS51117. LAMININ_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNTNG2_MOUSE
AccessioniPrimary (citable) accession number: Q8R4F1
Secondary accession number(s): A2AKX0
, Q8R4F2, Q8VIP6, Q8VIP7, Q8VIP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.