Q8R4E6 (PURG_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Purine-rich element-binding protein gamma

Gene names

Name:

Purg

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Nucleus By similarity.
Tissue specificity	Isoform 1 is expressed in testis. Isoform 2 is expressed in blastocyst and kidney. Ref.1
Sequence similarities	Belongs to the PUR DNA-binding protein family.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8R4E6-1)

Also known as: PURG-A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8R4E6-2)

Also known as: PURG-A;

The sequence of this isoform differs from the canonical sequence as follows:
     292-350: VSEVRPPYRN...ASGEEQECLD → LTNYPKSRENLNLFHCCQTKHKEQPYDTPKTVEE

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	Purine-rich element-binding protein gamma		PRO_0000255953
Regions
	DNA binding	54 – 296	243	By similarity
	Compositional bias	8 – 27	20	Gly-rich
Amino acid modifications
	Modified residue	163	1	Phosphoserine Ref.4
	Modified residue	166	1	Phosphoserine Ref.4
	Modified residue	342	1	Phosphoserine Ref.3
Natural variations
	Alternative sequence	292 – 350	59	VSEVR…QECLD → LTNYPKSRENLNLFHCCQTK HKEQPYDTPKTVEE in isoform 2.		VSP_021320
Experimental info
	Sequence conflict	222	1	D → E in AAI16407. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (PURG-A) [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 983142173937D7D4	FASTA	350	39,938
	10 20 30 40 50 60 MERARRRGGG GSGGGRGRGG KNVGGPGLSK SRLYPQAQHS HYPHYSASAT PNQSGGTSEI 70 80 90 100 110 120 QELASKRVDI QKKRFYLDVK QSSRGRFLKI AEVWIGRGRQ DNIRKSKLTL SLSVAAELKD 130 140 150 160 170 180 CLGDFIEHYA HLGLKGHRQE HGQSKEQVSR RRQKHSAPSP PVSVGSEEHP HSVLKTDYIE 190 200 210 220 230 240 RDNRKYYLDL KENQRGRFLR IRQTMMRGTG MIGYFGHSLG QDQTIVLPAQ GMIEFRDALV 250 260 270 280 290 300 QLIEDYGEGD IEERRCGDDD PLELPEGTSF RVDNKRFYFD VGSNKYGIFL KVSEVRPPYR 310 320 330 340 350 NTITVPFKAW TRFGENFIKY EEEMRKICNS HKEKRMDGRR ASGEEQECLD
	Isoform 2 (PURG-A) [UniParc]. Checksum: 57D07EF6AD8967AD	FASTA	325	36,936
	10 20 30 40 50 60 MERARRRGGG GSGGGRGRGG KNVGGPGLSK SRLYPQAQHS HYPHYSASAT PNQSGGTSEI 70 80 90 100 110 120 QELASKRVDI QKKRFYLDVK QSSRGRFLKI AEVWIGRGRQ DNIRKSKLTL SLSVAAELKD 130 140 150 160 170 180 CLGDFIEHYA HLGLKGHRQE HGQSKEQVSR RRQKHSAPSP PVSVGSEEHP HSVLKTDYIE 190 200 210 220 230 240 RDNRKYYLDL KENQRGRFLR IRQTMMRGTG MIGYFGHSLG QDQTIVLPAQ GMIEFRDALV 250 260 270 280 290 300 QLIEDYGEGD IEERRCGDDD PLELPEGTSF RVDNKRFYFD VGSNKYGIFL KLTNYPKSRE 310 320 NLNLFHCCQT KHKEQPYDTP KTVEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Distinct proteins encoded by alternative transcripts of the PURG gene, located contrapodal to WRN on chromosome 8, determined by differential termination/polyadenylation." Liu H., Johnson E.M. Nucleic Acids Res. 30:2417-2426(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY. Strain: B6D2 x J F1 and C57BL/6J. Tissue: Testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"Comprehensive identification of phosphorylation sites in postsynaptic density preparations." Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-342, MASS SPECTROMETRY. Tissue: Brain.
[4]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-163 AND SER-166, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF479672 mRNA. Translation: AAL86450.1. AF479673 mRNA. Translation: AAL86451.1. BC116406 mRNA. Translation: AAI16407.1.
IPI	IPI00153880. IPI00408500.
RefSeq	NP_001091703.1. NM_001098233.1. NP_690034.1. NM_152821.2.
UniGene	Mm.159402.
3D structure databases
ProteinModelPortal	Q8R4E6.
SMR	Q8R4E6. Positions 61-323.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000065002.
PTM databases
PhosphoSite	Q8R4E6.
Proteomic databases
PaxDb	Q8R4E6.
PRIDE	Q8R4E6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000070340; ENSMUSP00000065002; ENSMUSG00000049184. ENSMUST00000078058; ENSMUSP00000077205; ENSMUSG00000049184.
GeneID	75029.
KEGG	mmu:75029.
UCSC	uc009ljx.1. mouse. uc009ljy.1. mouse.
Organism-specific databases
CTD	29942.
MGI	MGI:1922279. Purg.
Phylogenomic databases
eggNOG	NOG312871.
GeneTree	ENSGT00390000015406.
HOGENOM	HOG000232132.
HOVERGEN	HBG006888.
InParanoid	Q8R4E6.
OMA	FGHTLGQ.
OrthoDB	EOG4PRSRB.
Gene expression databases
Bgee	Q8R4E6.
CleanEx	MM_PURG.
Genevestigator	Q8R4E6.
GermOnline	ENSMUSG00000049184. Mus musculus.
Family and domain databases
InterPro	IPR006628. PUR_DNA_RNA-bd. [Graphical view]
PANTHER	PTHR12611. PTHR12611. 1 hit.
Pfam	PF04845. PurA. 1 hit. [Graphical view]
SMART	SM00712. PUR. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	342048.
SOURCE	Search...

Entry information

Entry name

PURG_MOUSE

Accession

Primary (citable) accession number: Q8R4E6
Secondary accession number(s): Q14B11, Q8R4E7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program