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Q8R4D1 (SL9A8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium/hydrogen exchanger 8
Alternative name(s):
Na(+)/H(+) exchanger 8
Short name=NHE-8
Solute carrier family 9 member 8
Gene names
Name:Slc9a8
Synonyms:Nhe8
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length576 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction By similarity.

Subcellular location

Golgi apparatus membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Ubiquitously expressed. Expressed in both renal cortex and medulla. In the cortex, present in the majority of cortical tubules. In the medulla, most highly expressed in the proximal tubules (S3) of the outer stripe of the outer medulla. Ref.1

Sequence similarities

Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. [View classification]

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8R4D1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8R4D1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     65-91: Missing.
Isoform 3 (identifier: Q8R4D1-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-289: Missing.
     290-315: PSLEFGMMIIFAYLPYGLAEGISLSG → MWTLCSTCWCLEKAFSTMQSPSSSPS
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 576576Sodium/hydrogen exchanger 8
PRO_0000052366

Regions

Transmembrane55 – 7521Helical; Potential
Transmembrane79 – 9921Helical; Potential
Transmembrane118 – 13821Helical; Potential
Transmembrane151 – 17121Helical; Potential
Transmembrane186 – 20621Helical; Potential
Transmembrane211 – 23121Helical; Potential
Transmembrane256 – 27621Helical; Potential
Transmembrane306 – 32621Helical; Potential
Transmembrane349 – 36921Helical; Potential
Transmembrane374 – 39421Helical; Potential
Transmembrane412 – 43221Helical; Potential
Transmembrane446 – 46621Helical; Potential

Amino acid modifications

Modified residue5661Phosphoserine By similarity
Modified residue5681Phosphoserine By similarity

Natural variations

Alternative sequence1 – 289289Missing in isoform 3.
VSP_009503
Alternative sequence65 – 9127Missing in isoform 2.
VSP_009500
Alternative sequence290 – 31526PSLEF…ISLSG → MWTLCSTCWCLEKAFSTMQS PSSSPS in isoform 3.
VSP_009504

Experimental info

Sequence conflict2471S → R in BAE25331. Ref.3
Sequence conflict280 – 2834Missing in BAC34770. Ref.3
Sequence conflict5421D → E in BAE25331. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 8322845FDBAC1F8D

FASTA57664,737
        10         20         30         40         50         60 
MAEEEFSNTT HETFNFTLHT TLGVTTKLVL PTPAKPILPV QTGEQAQQEE QSSGMTIFFS 

        70         80         90        100        110        120 
LLVLAICIIL VHLLIRYRLH FLPESVAVVS LGILMGAVIK VIEFKKLANW KEEEMFRPNM 

       130        140        150        160        170        180 
FFLLLLPPII FESGYSLHKG NFFQNIGSIT LFAVFGTAIS AFVVGGGIYF LGQADVISKL 

       190        200        210        220        230        240 
NMTDSFAFGS LISAVDPVAT IAIFNALHVD PVLNMLVFGE SILNDAVSIV LTNTAEGLTR 

       250        260        270        280        290        300 
KHMSDVSGWQ TFSQALGYFL KMFFGSAALG TLTGLISALV LKHIDLRKTP SLEFGMMIIF 

       310        320        330        340        350        360 
AYLPYGLAEG ISLSGIMAIL FSGIVMSHYT HHNLSPVTQI LMQQTLRTVA FLCETCVFAF 

       370        380        390        400        410        420 
LGLSIFSFPH KFEISFVIWC IVLVLFGRAV NIFPLSYLLN FFRDHKITPK MMFIMWFSGL 

       430        440        450        460        470        480 
RGAIPYALSL HLGLEPMEKR QLIGTTTIVI VLFTILLLGG STMPLIRLVD IEDARARRRS 

       490        500        510        520        530        540 
KKDVNLSKTE KMGNAIESEH LSELTEEEYE AHYIRQQDLK GFMWLDAKYL NPFFTRRLTQ 

       550        560        570 
EDLHHGRIQM KSLTNKWYEE VRQGPSGSED DEQELF 

« Hide

Isoform 2 [UniParc].

Checksum: 8E715F0A659F2FD4
Show »

FASTA54961,666
Isoform 3 [UniParc].

Checksum: 0C465B32192E04A8
Show »

FASTA28733,222

References

« Hide 'large scale' references
[1]"Renal expression of novel Na+/H+ exchanger isoform NHE8."
Goyal S., Vanden Heuvel G., Aronson P.S.
Am. J. Physiol. 284:F467-F473(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
Strain: Swiss.
Tissue: Kidney.
[2]"Four Na+/H+ exchanger isoforms are distributed to Golgi and post-Golgi Compartments and are involved in organelle pH regulation."
Nakamura N., Tanaka S., Teko Y., Mitsui K., Kanazawa H.
J. Biol. Chem. 280:1561-1572(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Kidney.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Embryonic spinal ganglion and Oviduct.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[5]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[6]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6 and FVB/N.
Tissue: Brain and Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF482993 mRNA. Translation: AAL89753.1.
AB089793 mRNA. Translation: BAD69591.1.
AK051791 mRNA. Translation: BAC34770.1.
AK129013 mRNA. Translation: BAC87669.1.
AK143281 mRNA. Translation: BAE25331.1.
AL589870, AL591762 Genomic DNA. Translation: CAM13827.1.
CH466551 Genomic DNA. Translation: EDL06513.1.
BC030879 mRNA. Translation: AAH30879.1.
BC058947 mRNA. Translation: AAH58947.1.
CCDSCCDS38339.1. [Q8R4D1-1]
CCDS38340.1. [Q8R4D1-2]
RefSeqNP_683731.1. NM_148929.2. [Q8R4D1-1]
NP_848458.2. NM_178371.3. [Q8R4D1-2]
UniGeneMm.278924.

3D structure databases

ProteinModelPortalQ8R4D1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000044185.

PTM databases

PhosphoSiteQ8R4D1.

Proteomic databases

MaxQBQ8R4D1.
PRIDEQ8R4D1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000047815; ENSMUSP00000044185; ENSMUSG00000039463. [Q8R4D1-1]
ENSMUST00000073873; ENSMUSP00000073536; ENSMUSG00000039463. [Q8R4D1-2]
GeneID77031.
KEGGmmu:77031.
UCSCuc008nzp.1. mouse. [Q8R4D1-1]
uc008nzq.1. mouse. [Q8R4D1-2]
uc008nzr.1. mouse. [Q8R4D1-4]

Organism-specific databases

CTD23315.
MGIMGI:1924281. Slc9a8.

Phylogenomic databases

eggNOGCOG0025.
GeneTreeENSGT00560000076802.
HOGENOMHOG000172307.
HOVERGENHBG056815.
InParanoidA2A465.
KOK14724.
OMAQKMGNTI.
OrthoDBEOG7H4DT6.
PhylomeDBQ8R4D1.
TreeFamTF354313.

Gene expression databases

ArrayExpressQ8R4D1.
BgeeQ8R4D1.
GenevestigatorQ8R4D1.

Family and domain databases

InterProIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR018409. Na/H_exchanger_8.
IPR004709. NaH_exchanger.
[Graphical view]
PANTHERPTHR10110. PTHR10110. 1 hit.
PTHR10110:SF88. PTHR10110:SF88. 1 hit.
PfamPF00999. Na_H_Exchanger. 1 hit.
[Graphical view]
PRINTSPR01084. NAHEXCHNGR.
TIGRFAMsTIGR00840. b_cpa1. 1 hit.
ProtoNetSearch...

Other

NextBio346322.
PROQ8R4D1.
SOURCESearch...

Entry information

Entry nameSL9A8_MOUSE
AccessionPrimary (citable) accession number: Q8R4D1
Secondary accession number(s): A2A465 expand/collapse secondary AC list , Q3UPR4, Q5WA59, Q8BIH8, Q8BJ27
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot