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Protein

Sodium/hydrogen exchanger 8

Gene

Slc9a8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction (By similarity).By similarity

GO - Molecular functioni

  • potassium:proton antiporter activity Source: MGI
  • sodium:proton antiporter activity Source: MGI

GO - Biological processi

  • potassium ion transport Source: MGI
  • regulation of intracellular pH Source: MGI
  • sodium ion transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Sodium transport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiR-MMU-425986. Sodium/Proton exchangers.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/hydrogen exchanger 8
Alternative name(s):
Na(+)/H(+) exchanger 8
Short name:
NHE-8
Solute carrier family 9 member 8
Gene namesi
Name:Slc9a8
Synonyms:Nhe8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1924281. Slc9a8.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei55 – 75HelicalSequence analysisAdd BLAST21
Transmembranei79 – 99HelicalSequence analysisAdd BLAST21
Transmembranei118 – 138HelicalSequence analysisAdd BLAST21
Transmembranei151 – 171HelicalSequence analysisAdd BLAST21
Transmembranei186 – 206HelicalSequence analysisAdd BLAST21
Transmembranei211 – 231HelicalSequence analysisAdd BLAST21
Transmembranei256 – 276HelicalSequence analysisAdd BLAST21
Transmembranei306 – 326HelicalSequence analysisAdd BLAST21
Transmembranei349 – 369HelicalSequence analysisAdd BLAST21
Transmembranei374 – 394HelicalSequence analysisAdd BLAST21
Transmembranei412 – 432HelicalSequence analysisAdd BLAST21
Transmembranei446 – 466HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • Golgi apparatus Source: MGI
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000523661 – 576Sodium/hydrogen exchanger 8Add BLAST576

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei505PhosphothreonineCombined sources1
Modified residuei566PhosphoserineBy similarity1
Modified residuei568PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8R4D1.
PRIDEiQ8R4D1.

PTM databases

iPTMnetiQ8R4D1.
PhosphoSitePlusiQ8R4D1.

Expressioni

Tissue specificityi

Ubiquitously expressed. Expressed in both renal cortex and medulla. In the cortex, present in the majority of cortical tubules. In the medulla, most highly expressed in the proximal tubules (S3) of the outer stripe of the outer medulla.1 Publication

Gene expression databases

BgeeiENSMUSG00000039463.
ExpressionAtlasiQ8R4D1. baseline and differential.
GenevisibleiQ8R4D1. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044185.

Structurei

3D structure databases

ProteinModelPortaliQ8R4D1.
SMRiQ8R4D1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1965. Eukaryota.
COG0025. LUCA.
GeneTreeiENSGT00760000119074.
HOGENOMiHOG000172307.
HOVERGENiHBG056815.
InParanoidiQ8R4D1.
KOiK14724.
OMAiEFSNTTH.
OrthoDBiEOG091G05T4.
PhylomeDBiQ8R4D1.
TreeFamiTF354313.

Family and domain databases

InterProiIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR018409. Na/H_exchanger_8.
IPR004709. NaH_exchanger.
[Graphical view]
PANTHERiPTHR10110. PTHR10110. 1 hit.
PTHR10110:SF122. PTHR10110:SF122. 1 hit.
PfamiPF00999. Na_H_Exchanger. 1 hit.
[Graphical view]
PRINTSiPR01084. NAHEXCHNGR.
TIGRFAMsiTIGR00840. b_cpa1. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R4D1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEEEFSNTT HETFNFTLHT TLGVTTKLVL PTPAKPILPV QTGEQAQQEE
60 70 80 90 100
QSSGMTIFFS LLVLAICIIL VHLLIRYRLH FLPESVAVVS LGILMGAVIK
110 120 130 140 150
VIEFKKLANW KEEEMFRPNM FFLLLLPPII FESGYSLHKG NFFQNIGSIT
160 170 180 190 200
LFAVFGTAIS AFVVGGGIYF LGQADVISKL NMTDSFAFGS LISAVDPVAT
210 220 230 240 250
IAIFNALHVD PVLNMLVFGE SILNDAVSIV LTNTAEGLTR KHMSDVSGWQ
260 270 280 290 300
TFSQALGYFL KMFFGSAALG TLTGLISALV LKHIDLRKTP SLEFGMMIIF
310 320 330 340 350
AYLPYGLAEG ISLSGIMAIL FSGIVMSHYT HHNLSPVTQI LMQQTLRTVA
360 370 380 390 400
FLCETCVFAF LGLSIFSFPH KFEISFVIWC IVLVLFGRAV NIFPLSYLLN
410 420 430 440 450
FFRDHKITPK MMFIMWFSGL RGAIPYALSL HLGLEPMEKR QLIGTTTIVI
460 470 480 490 500
VLFTILLLGG STMPLIRLVD IEDARARRRS KKDVNLSKTE KMGNAIESEH
510 520 530 540 550
LSELTEEEYE AHYIRQQDLK GFMWLDAKYL NPFFTRRLTQ EDLHHGRIQM
560 570
KSLTNKWYEE VRQGPSGSED DEQELF
Length:576
Mass (Da):64,737
Last modified:June 1, 2002 - v1
Checksum:i8322845FDBAC1F8D
GO
Isoform 2 (identifier: Q8R4D1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     65-91: Missing.

Show »
Length:549
Mass (Da):61,666
Checksum:i8E715F0A659F2FD4
GO
Isoform 3 (identifier: Q8R4D1-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-289: Missing.
     290-315: PSLEFGMMIIFAYLPYGLAEGISLSG → MWTLCSTCWCLEKAFSTMQSPSSSPS

Note: No experimental confirmation available.
Show »
Length:287
Mass (Da):33,222
Checksum:i0C465B32192E04A8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti247S → R in BAE25331 (PubMed:16141072).Curated1
Sequence conflicti280 – 283Missing in BAC34770 (PubMed:16141072).Curated4
Sequence conflicti542D → E in BAE25331 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0095031 – 289Missing in isoform 3. 1 PublicationAdd BLAST289
Alternative sequenceiVSP_00950065 – 91Missing in isoform 2. 1 PublicationAdd BLAST27
Alternative sequenceiVSP_009504290 – 315PSLEF…ISLSG → MWTLCSTCWCLEKAFSTMQS PSSSPS in isoform 3. 1 PublicationAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF482993 mRNA. Translation: AAL89753.1.
AB089793 mRNA. Translation: BAD69591.1.
AK051791 mRNA. Translation: BAC34770.1.
AK129013 mRNA. Translation: BAC87669.1.
AK143281 mRNA. Translation: BAE25331.1.
AL589870, AL591762 Genomic DNA. Translation: CAM13827.1.
CH466551 Genomic DNA. Translation: EDL06513.1.
BC030879 mRNA. Translation: AAH30879.1.
BC058947 mRNA. Translation: AAH58947.1.
CCDSiCCDS38339.1. [Q8R4D1-1]
RefSeqiNP_001291469.1. NM_001304540.1.
NP_001291471.1. NM_001304542.1.
NP_683731.1. NM_148929.3. [Q8R4D1-1]
UniGeneiMm.278924.

Genome annotation databases

EnsembliENSMUST00000047815; ENSMUSP00000044185; ENSMUSG00000039463. [Q8R4D1-1]
ENSMUST00000073873; ENSMUSP00000073536; ENSMUSG00000039463. [Q8R4D1-2]
GeneIDi77031.
KEGGimmu:77031.
UCSCiuc008nzp.2. mouse. [Q8R4D1-1]
uc008nzr.2. mouse. [Q8R4D1-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF482993 mRNA. Translation: AAL89753.1.
AB089793 mRNA. Translation: BAD69591.1.
AK051791 mRNA. Translation: BAC34770.1.
AK129013 mRNA. Translation: BAC87669.1.
AK143281 mRNA. Translation: BAE25331.1.
AL589870, AL591762 Genomic DNA. Translation: CAM13827.1.
CH466551 Genomic DNA. Translation: EDL06513.1.
BC030879 mRNA. Translation: AAH30879.1.
BC058947 mRNA. Translation: AAH58947.1.
CCDSiCCDS38339.1. [Q8R4D1-1]
RefSeqiNP_001291469.1. NM_001304540.1.
NP_001291471.1. NM_001304542.1.
NP_683731.1. NM_148929.3. [Q8R4D1-1]
UniGeneiMm.278924.

3D structure databases

ProteinModelPortaliQ8R4D1.
SMRiQ8R4D1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044185.

PTM databases

iPTMnetiQ8R4D1.
PhosphoSitePlusiQ8R4D1.

Proteomic databases

PaxDbiQ8R4D1.
PRIDEiQ8R4D1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047815; ENSMUSP00000044185; ENSMUSG00000039463. [Q8R4D1-1]
ENSMUST00000073873; ENSMUSP00000073536; ENSMUSG00000039463. [Q8R4D1-2]
GeneIDi77031.
KEGGimmu:77031.
UCSCiuc008nzp.2. mouse. [Q8R4D1-1]
uc008nzr.2. mouse. [Q8R4D1-4]

Organism-specific databases

CTDi23315.
MGIiMGI:1924281. Slc9a8.

Phylogenomic databases

eggNOGiKOG1965. Eukaryota.
COG0025. LUCA.
GeneTreeiENSGT00760000119074.
HOGENOMiHOG000172307.
HOVERGENiHBG056815.
InParanoidiQ8R4D1.
KOiK14724.
OMAiEFSNTTH.
OrthoDBiEOG091G05T4.
PhylomeDBiQ8R4D1.
TreeFamiTF354313.

Enzyme and pathway databases

ReactomeiR-MMU-425986. Sodium/Proton exchangers.

Miscellaneous databases

PROiQ8R4D1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039463.
ExpressionAtlasiQ8R4D1. baseline and differential.
GenevisibleiQ8R4D1. MM.

Family and domain databases

InterProiIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR018409. Na/H_exchanger_8.
IPR004709. NaH_exchanger.
[Graphical view]
PANTHERiPTHR10110. PTHR10110. 1 hit.
PTHR10110:SF122. PTHR10110:SF122. 1 hit.
PfamiPF00999. Na_H_Exchanger. 1 hit.
[Graphical view]
PRINTSiPR01084. NAHEXCHNGR.
TIGRFAMsiTIGR00840. b_cpa1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSL9A8_MOUSE
AccessioniPrimary (citable) accession number: Q8R4D1
Secondary accession number(s): A2A465
, Q3UPR4, Q5WA59, Q8BIH8, Q8BJ27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: June 1, 2002
Last modified: November 2, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.