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Q8R4D1

- SL9A8_MOUSE

UniProt

Q8R4D1 - SL9A8_MOUSE

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Protein

Sodium/hydrogen exchanger 8

Gene
Slc9a8, Nhe8
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction By similarity.

GO - Molecular functioni

  1. potassium:proton antiporter activity Source: MGI
  2. sodium:proton antiporter activity Source: MGI

GO - Biological processi

  1. potassium ion transmembrane transport Source: GOC
  2. potassium ion transport Source: MGI
  3. regulation of intracellular pH Source: MGI
  4. sodium ion transmembrane transport Source: GOC
  5. sodium ion transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Sodium transport, Transport

Keywords - Ligandi

Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/hydrogen exchanger 8
Alternative name(s):
Na(+)/H(+) exchanger 8
Short name:
NHE-8
Solute carrier family 9 member 8
Gene namesi
Name:Slc9a8
Synonyms:Nhe8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:1924281. Slc9a8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei55 – 7521Helical; Reviewed predictionAdd
BLAST
Transmembranei79 – 9921Helical; Reviewed predictionAdd
BLAST
Transmembranei118 – 13821Helical; Reviewed predictionAdd
BLAST
Transmembranei151 – 17121Helical; Reviewed predictionAdd
BLAST
Transmembranei186 – 20621Helical; Reviewed predictionAdd
BLAST
Transmembranei211 – 23121Helical; Reviewed predictionAdd
BLAST
Transmembranei256 – 27621Helical; Reviewed predictionAdd
BLAST
Transmembranei306 – 32621Helical; Reviewed predictionAdd
BLAST
Transmembranei349 – 36921Helical; Reviewed predictionAdd
BLAST
Transmembranei374 – 39421Helical; Reviewed predictionAdd
BLAST
Transmembranei412 – 43221Helical; Reviewed predictionAdd
BLAST
Transmembranei446 – 46621Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: MGI
  2. Golgi membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 576576Sodium/hydrogen exchanger 8PRO_0000052366Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei566 – 5661Phosphoserine By similarity
Modified residuei568 – 5681Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8R4D1.
PRIDEiQ8R4D1.

PTM databases

PhosphoSiteiQ8R4D1.

Expressioni

Tissue specificityi

Ubiquitously expressed. Expressed in both renal cortex and medulla. In the cortex, present in the majority of cortical tubules. In the medulla, most highly expressed in the proximal tubules (S3) of the outer stripe of the outer medulla.1 Publication

Gene expression databases

ArrayExpressiQ8R4D1.
BgeeiQ8R4D1.
GenevestigatoriQ8R4D1.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044185.

Structurei

3D structure databases

ProteinModelPortaliQ8R4D1.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0025.
GeneTreeiENSGT00560000076802.
HOGENOMiHOG000172307.
HOVERGENiHBG056815.
InParanoidiA2A465.
KOiK14724.
OMAiQKMGNTI.
OrthoDBiEOG7H4DT6.
PhylomeDBiQ8R4D1.
TreeFamiTF354313.

Family and domain databases

InterProiIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR018409. Na/H_exchanger_8.
IPR004709. NaH_exchanger.
[Graphical view]
PANTHERiPTHR10110. PTHR10110. 1 hit.
PTHR10110:SF88. PTHR10110:SF88. 1 hit.
PfamiPF00999. Na_H_Exchanger. 1 hit.
[Graphical view]
PRINTSiPR01084. NAHEXCHNGR.
TIGRFAMsiTIGR00840. b_cpa1. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8R4D1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAEEEFSNTT HETFNFTLHT TLGVTTKLVL PTPAKPILPV QTGEQAQQEE    50
QSSGMTIFFS LLVLAICIIL VHLLIRYRLH FLPESVAVVS LGILMGAVIK 100
VIEFKKLANW KEEEMFRPNM FFLLLLPPII FESGYSLHKG NFFQNIGSIT 150
LFAVFGTAIS AFVVGGGIYF LGQADVISKL NMTDSFAFGS LISAVDPVAT 200
IAIFNALHVD PVLNMLVFGE SILNDAVSIV LTNTAEGLTR KHMSDVSGWQ 250
TFSQALGYFL KMFFGSAALG TLTGLISALV LKHIDLRKTP SLEFGMMIIF 300
AYLPYGLAEG ISLSGIMAIL FSGIVMSHYT HHNLSPVTQI LMQQTLRTVA 350
FLCETCVFAF LGLSIFSFPH KFEISFVIWC IVLVLFGRAV NIFPLSYLLN 400
FFRDHKITPK MMFIMWFSGL RGAIPYALSL HLGLEPMEKR QLIGTTTIVI 450
VLFTILLLGG STMPLIRLVD IEDARARRRS KKDVNLSKTE KMGNAIESEH 500
LSELTEEEYE AHYIRQQDLK GFMWLDAKYL NPFFTRRLTQ EDLHHGRIQM 550
KSLTNKWYEE VRQGPSGSED DEQELF 576
Length:576
Mass (Da):64,737
Last modified:June 1, 2002 - v1
Checksum:i8322845FDBAC1F8D
GO
Isoform 2 (identifier: Q8R4D1-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     65-91: Missing.

Show »
Length:549
Mass (Da):61,666
Checksum:i8E715F0A659F2FD4
GO
Isoform 3 (identifier: Q8R4D1-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-289: Missing.
     290-315: PSLEFGMMIIFAYLPYGLAEGISLSG → MWTLCSTCWCLEKAFSTMQSPSSSPS

Note: No experimental confirmation available.

Show »
Length:287
Mass (Da):33,222
Checksum:i0C465B32192E04A8
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 289289Missing in isoform 3. VSP_009503Add
BLAST
Alternative sequencei65 – 9127Missing in isoform 2. VSP_009500Add
BLAST
Alternative sequencei290 – 31526PSLEF…ISLSG → MWTLCSTCWCLEKAFSTMQS PSSSPS in isoform 3. VSP_009504Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti247 – 2471S → R in BAE25331. 1 Publication
Sequence conflicti280 – 2834Missing in BAC34770. 1 Publication
Sequence conflicti542 – 5421D → E in BAE25331. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF482993 mRNA. Translation: AAL89753.1.
AB089793 mRNA. Translation: BAD69591.1.
AK051791 mRNA. Translation: BAC34770.1.
AK129013 mRNA. Translation: BAC87669.1.
AK143281 mRNA. Translation: BAE25331.1.
AL589870, AL591762 Genomic DNA. Translation: CAM13827.1.
CH466551 Genomic DNA. Translation: EDL06513.1.
BC030879 mRNA. Translation: AAH30879.1.
BC058947 mRNA. Translation: AAH58947.1.
CCDSiCCDS38339.1. [Q8R4D1-1]
CCDS38340.1. [Q8R4D1-2]
RefSeqiNP_683731.1. NM_148929.2. [Q8R4D1-1]
NP_848458.2. NM_178371.3. [Q8R4D1-2]
UniGeneiMm.278924.

Genome annotation databases

EnsembliENSMUST00000047815; ENSMUSP00000044185; ENSMUSG00000039463. [Q8R4D1-1]
ENSMUST00000073873; ENSMUSP00000073536; ENSMUSG00000039463. [Q8R4D1-2]
GeneIDi77031.
KEGGimmu:77031.
UCSCiuc008nzp.1. mouse. [Q8R4D1-1]
uc008nzq.1. mouse. [Q8R4D1-2]
uc008nzr.1. mouse. [Q8R4D1-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF482993 mRNA. Translation: AAL89753.1 .
AB089793 mRNA. Translation: BAD69591.1 .
AK051791 mRNA. Translation: BAC34770.1 .
AK129013 mRNA. Translation: BAC87669.1 .
AK143281 mRNA. Translation: BAE25331.1 .
AL589870 , AL591762 Genomic DNA. Translation: CAM13827.1 .
CH466551 Genomic DNA. Translation: EDL06513.1 .
BC030879 mRNA. Translation: AAH30879.1 .
BC058947 mRNA. Translation: AAH58947.1 .
CCDSi CCDS38339.1. [Q8R4D1-1 ]
CCDS38340.1. [Q8R4D1-2 ]
RefSeqi NP_683731.1. NM_148929.2. [Q8R4D1-1 ]
NP_848458.2. NM_178371.3. [Q8R4D1-2 ]
UniGenei Mm.278924.

3D structure databases

ProteinModelPortali Q8R4D1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000044185.

PTM databases

PhosphoSitei Q8R4D1.

Proteomic databases

MaxQBi Q8R4D1.
PRIDEi Q8R4D1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000047815 ; ENSMUSP00000044185 ; ENSMUSG00000039463 . [Q8R4D1-1 ]
ENSMUST00000073873 ; ENSMUSP00000073536 ; ENSMUSG00000039463 . [Q8R4D1-2 ]
GeneIDi 77031.
KEGGi mmu:77031.
UCSCi uc008nzp.1. mouse. [Q8R4D1-1 ]
uc008nzq.1. mouse. [Q8R4D1-2 ]
uc008nzr.1. mouse. [Q8R4D1-4 ]

Organism-specific databases

CTDi 23315.
MGIi MGI:1924281. Slc9a8.

Phylogenomic databases

eggNOGi COG0025.
GeneTreei ENSGT00560000076802.
HOGENOMi HOG000172307.
HOVERGENi HBG056815.
InParanoidi A2A465.
KOi K14724.
OMAi QKMGNTI.
OrthoDBi EOG7H4DT6.
PhylomeDBi Q8R4D1.
TreeFami TF354313.

Miscellaneous databases

NextBioi 346322.
PROi Q8R4D1.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8R4D1.
Bgeei Q8R4D1.
Genevestigatori Q8R4D1.

Family and domain databases

InterProi IPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR018409. Na/H_exchanger_8.
IPR004709. NaH_exchanger.
[Graphical view ]
PANTHERi PTHR10110. PTHR10110. 1 hit.
PTHR10110:SF88. PTHR10110:SF88. 1 hit.
Pfami PF00999. Na_H_Exchanger. 1 hit.
[Graphical view ]
PRINTSi PR01084. NAHEXCHNGR.
TIGRFAMsi TIGR00840. b_cpa1. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Renal expression of novel Na+/H+ exchanger isoform NHE8."
    Goyal S., Vanden Heuvel G., Aronson P.S.
    Am. J. Physiol. 284:F467-F473(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: Swiss.
    Tissue: Kidney.
  2. "Four Na+/H+ exchanger isoforms are distributed to Golgi and post-Golgi Compartments and are involved in organelle pH regulation."
    Nakamura N., Tanaka S., Teko Y., Mitsui K., Kanazawa H.
    J. Biol. Chem. 280:1561-1572(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Kidney.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Embryonic spinal ganglion and Oviduct.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  6. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6 and FVB/N.
    Tissue: Brain and Kidney.

Entry informationi

Entry nameiSL9A8_MOUSE
AccessioniPrimary (citable) accession number: Q8R4D1
Secondary accession number(s): A2A465
, Q3UPR4, Q5WA59, Q8BIH8, Q8BJ27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi