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Q8R4D1

- SL9A8_MOUSE

UniProt

Q8R4D1 - SL9A8_MOUSE

Protein

Sodium/hydrogen exchanger 8

Gene

Slc9a8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction By similarity.By similarity

    GO - Molecular functioni

    1. potassium:proton antiporter activity Source: MGI
    2. sodium:proton antiporter activity Source: MGI

    GO - Biological processi

    1. potassium ion transmembrane transport Source: GOC
    2. potassium ion transport Source: MGI
    3. regulation of intracellular pH Source: MGI
    4. sodium ion transmembrane transport Source: GOC
    5. sodium ion transport Source: MGI

    Keywords - Biological processi

    Antiport, Ion transport, Sodium transport, Transport

    Keywords - Ligandi

    Sodium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium/hydrogen exchanger 8
    Alternative name(s):
    Na(+)/H(+) exchanger 8
    Short name:
    NHE-8
    Solute carrier family 9 member 8
    Gene namesi
    Name:Slc9a8
    Synonyms:Nhe8
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1924281. Slc9a8.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi apparatus Source: MGI
    2. Golgi membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 576576Sodium/hydrogen exchanger 8PRO_0000052366Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei566 – 5661PhosphoserineBy similarity
    Modified residuei568 – 5681PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8R4D1.
    PRIDEiQ8R4D1.

    PTM databases

    PhosphoSiteiQ8R4D1.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed. Expressed in both renal cortex and medulla. In the cortex, present in the majority of cortical tubules. In the medulla, most highly expressed in the proximal tubules (S3) of the outer stripe of the outer medulla.1 Publication

    Gene expression databases

    ArrayExpressiQ8R4D1.
    BgeeiQ8R4D1.
    GenevestigatoriQ8R4D1.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000044185.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8R4D1.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei55 – 7521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei79 – 9921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei118 – 13821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei151 – 17121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei186 – 20621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei211 – 23121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei256 – 27621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei306 – 32621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei349 – 36921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei374 – 39421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei412 – 43221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei446 – 46621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0025.
    GeneTreeiENSGT00560000076802.
    HOGENOMiHOG000172307.
    HOVERGENiHBG056815.
    InParanoidiA2A465.
    KOiK14724.
    OMAiQKMGNTI.
    OrthoDBiEOG7H4DT6.
    PhylomeDBiQ8R4D1.
    TreeFamiTF354313.

    Family and domain databases

    InterProiIPR006153. Cation/H_exchanger.
    IPR018422. Cation/H_exchanger_CPA1.
    IPR018409. Na/H_exchanger_8.
    IPR004709. NaH_exchanger.
    [Graphical view]
    PANTHERiPTHR10110. PTHR10110. 1 hit.
    PTHR10110:SF88. PTHR10110:SF88. 1 hit.
    PfamiPF00999. Na_H_Exchanger. 1 hit.
    [Graphical view]
    PRINTSiPR01084. NAHEXCHNGR.
    TIGRFAMsiTIGR00840. b_cpa1. 1 hit.

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8R4D1-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAEEEFSNTT HETFNFTLHT TLGVTTKLVL PTPAKPILPV QTGEQAQQEE    50
    QSSGMTIFFS LLVLAICIIL VHLLIRYRLH FLPESVAVVS LGILMGAVIK 100
    VIEFKKLANW KEEEMFRPNM FFLLLLPPII FESGYSLHKG NFFQNIGSIT 150
    LFAVFGTAIS AFVVGGGIYF LGQADVISKL NMTDSFAFGS LISAVDPVAT 200
    IAIFNALHVD PVLNMLVFGE SILNDAVSIV LTNTAEGLTR KHMSDVSGWQ 250
    TFSQALGYFL KMFFGSAALG TLTGLISALV LKHIDLRKTP SLEFGMMIIF 300
    AYLPYGLAEG ISLSGIMAIL FSGIVMSHYT HHNLSPVTQI LMQQTLRTVA 350
    FLCETCVFAF LGLSIFSFPH KFEISFVIWC IVLVLFGRAV NIFPLSYLLN 400
    FFRDHKITPK MMFIMWFSGL RGAIPYALSL HLGLEPMEKR QLIGTTTIVI 450
    VLFTILLLGG STMPLIRLVD IEDARARRRS KKDVNLSKTE KMGNAIESEH 500
    LSELTEEEYE AHYIRQQDLK GFMWLDAKYL NPFFTRRLTQ EDLHHGRIQM 550
    KSLTNKWYEE VRQGPSGSED DEQELF 576
    Length:576
    Mass (Da):64,737
    Last modified:June 1, 2002 - v1
    Checksum:i8322845FDBAC1F8D
    GO
    Isoform 2 (identifier: Q8R4D1-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         65-91: Missing.

    Show »
    Length:549
    Mass (Da):61,666
    Checksum:i8E715F0A659F2FD4
    GO
    Isoform 3 (identifier: Q8R4D1-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-289: Missing.
         290-315: PSLEFGMMIIFAYLPYGLAEGISLSG → MWTLCSTCWCLEKAFSTMQSPSSSPS

    Note: No experimental confirmation available.

    Show »
    Length:287
    Mass (Da):33,222
    Checksum:i0C465B32192E04A8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti247 – 2471S → R in BAE25331. (PubMed:16141072)Curated
    Sequence conflicti280 – 2834Missing in BAC34770. (PubMed:16141072)Curated
    Sequence conflicti542 – 5421D → E in BAE25331. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 289289Missing in isoform 3. 1 PublicationVSP_009503Add
    BLAST
    Alternative sequencei65 – 9127Missing in isoform 2. 1 PublicationVSP_009500Add
    BLAST
    Alternative sequencei290 – 31526PSLEF…ISLSG → MWTLCSTCWCLEKAFSTMQS PSSSPS in isoform 3. 1 PublicationVSP_009504Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF482993 mRNA. Translation: AAL89753.1.
    AB089793 mRNA. Translation: BAD69591.1.
    AK051791 mRNA. Translation: BAC34770.1.
    AK129013 mRNA. Translation: BAC87669.1.
    AK143281 mRNA. Translation: BAE25331.1.
    AL589870, AL591762 Genomic DNA. Translation: CAM13827.1.
    CH466551 Genomic DNA. Translation: EDL06513.1.
    BC030879 mRNA. Translation: AAH30879.1.
    BC058947 mRNA. Translation: AAH58947.1.
    CCDSiCCDS38339.1. [Q8R4D1-1]
    CCDS38340.1. [Q8R4D1-2]
    RefSeqiNP_683731.1. NM_148929.2. [Q8R4D1-1]
    NP_848458.2. NM_178371.3. [Q8R4D1-2]
    UniGeneiMm.278924.

    Genome annotation databases

    EnsembliENSMUST00000047815; ENSMUSP00000044185; ENSMUSG00000039463. [Q8R4D1-1]
    ENSMUST00000073873; ENSMUSP00000073536; ENSMUSG00000039463. [Q8R4D1-2]
    GeneIDi77031.
    KEGGimmu:77031.
    UCSCiuc008nzp.1. mouse. [Q8R4D1-1]
    uc008nzq.1. mouse. [Q8R4D1-2]
    uc008nzr.1. mouse. [Q8R4D1-4]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF482993 mRNA. Translation: AAL89753.1 .
    AB089793 mRNA. Translation: BAD69591.1 .
    AK051791 mRNA. Translation: BAC34770.1 .
    AK129013 mRNA. Translation: BAC87669.1 .
    AK143281 mRNA. Translation: BAE25331.1 .
    AL589870 , AL591762 Genomic DNA. Translation: CAM13827.1 .
    CH466551 Genomic DNA. Translation: EDL06513.1 .
    BC030879 mRNA. Translation: AAH30879.1 .
    BC058947 mRNA. Translation: AAH58947.1 .
    CCDSi CCDS38339.1. [Q8R4D1-1 ]
    CCDS38340.1. [Q8R4D1-2 ]
    RefSeqi NP_683731.1. NM_148929.2. [Q8R4D1-1 ]
    NP_848458.2. NM_178371.3. [Q8R4D1-2 ]
    UniGenei Mm.278924.

    3D structure databases

    ProteinModelPortali Q8R4D1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000044185.

    PTM databases

    PhosphoSitei Q8R4D1.

    Proteomic databases

    MaxQBi Q8R4D1.
    PRIDEi Q8R4D1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000047815 ; ENSMUSP00000044185 ; ENSMUSG00000039463 . [Q8R4D1-1 ]
    ENSMUST00000073873 ; ENSMUSP00000073536 ; ENSMUSG00000039463 . [Q8R4D1-2 ]
    GeneIDi 77031.
    KEGGi mmu:77031.
    UCSCi uc008nzp.1. mouse. [Q8R4D1-1 ]
    uc008nzq.1. mouse. [Q8R4D1-2 ]
    uc008nzr.1. mouse. [Q8R4D1-4 ]

    Organism-specific databases

    CTDi 23315.
    MGIi MGI:1924281. Slc9a8.

    Phylogenomic databases

    eggNOGi COG0025.
    GeneTreei ENSGT00560000076802.
    HOGENOMi HOG000172307.
    HOVERGENi HBG056815.
    InParanoidi A2A465.
    KOi K14724.
    OMAi QKMGNTI.
    OrthoDBi EOG7H4DT6.
    PhylomeDBi Q8R4D1.
    TreeFami TF354313.

    Miscellaneous databases

    NextBioi 346322.
    PROi Q8R4D1.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8R4D1.
    Bgeei Q8R4D1.
    Genevestigatori Q8R4D1.

    Family and domain databases

    InterProi IPR006153. Cation/H_exchanger.
    IPR018422. Cation/H_exchanger_CPA1.
    IPR018409. Na/H_exchanger_8.
    IPR004709. NaH_exchanger.
    [Graphical view ]
    PANTHERi PTHR10110. PTHR10110. 1 hit.
    PTHR10110:SF88. PTHR10110:SF88. 1 hit.
    Pfami PF00999. Na_H_Exchanger. 1 hit.
    [Graphical view ]
    PRINTSi PR01084. NAHEXCHNGR.
    TIGRFAMsi TIGR00840. b_cpa1. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Renal expression of novel Na+/H+ exchanger isoform NHE8."
      Goyal S., Vanden Heuvel G., Aronson P.S.
      Am. J. Physiol. 284:F467-F473(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
      Strain: Swiss.
      Tissue: Kidney.
    2. "Four Na+/H+ exchanger isoforms are distributed to Golgi and post-Golgi Compartments and are involved in organelle pH regulation."
      Nakamura N., Tanaka S., Teko Y., Mitsui K., Kanazawa H.
      J. Biol. Chem. 280:1561-1572(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Kidney.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Embryonic spinal ganglion and Oviduct.
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    6. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6 and FVB/N.
      Tissue: Brain and Kidney.

    Entry informationi

    Entry nameiSL9A8_MOUSE
    AccessioniPrimary (citable) accession number: Q8R4D1
    Secondary accession number(s): A2A465
    , Q3UPR4, Q5WA59, Q8BIH8, Q8BJ27
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2004
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3