ID   RUFY2_MOUSE             Reviewed;         606 AA.
AC   Q8R4C2; Q69ZH1;
DT   11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 2.
DT   27-MAR-2024, entry version 128.
DE   RecName: Full=RUN and FYVE domain-containing protein 2;
DE   AltName: Full=Leucine zipper FYVE-finger protein;
DE            Short=LZ-FYVE;
GN   Name=Rufy2; Synonyms=Kiaa1537;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, AND SUBCELLULAR LOCATION.
RC   TISSUE=Embryo;
RX   PubMed=11506704; DOI=10.1089/104454901750361460;
RA   Dunkelberg J.C., Gutierrez-Hartmann A.;
RT   "LZ-FYVE: a novel developmental stage-specific leucine zipper, FYVE-finger
RT   protein.";
RL   DNA Cell Biol. 20:403-412(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=BALB/cJ; TISSUE=Brain;
RX   PubMed=11877430; DOI=10.1074/jbc.m111933200;
RA   Yang J., Kim O., Wu J., Qiu Y.;
RT   "Interaction between tyrosine kinase Etk and a RUN-domain and FYVE-domain
RT   containing protein RUFY1. A possible role of Etk in regulation of vesicle
RT   trafficking.";
RL   J. Biol. Chem. 277:30219-30226(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15368895; DOI=10.1093/dnares/11.3.205;
RA   Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA   Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H.,
RA   Nagase T., Ohara O., Koga H.;
RT   "Prediction of the coding sequences of mouse homologues of KIAA gene: IV.
RT   The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT   identified by screening of terminal sequences of cDNA clones randomly
RT   sampled from size-fractionated libraries.";
RL   DNA Res. 11:205-218(2004).
CC   -!- SUBUNIT: Interacts with BMX. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11506704}.
CC   -!- DEVELOPMENTAL STAGE: Expressed only at embryonic stages. Present at 12
CC       dpc in primordial lung, cartilage and otic capsule (at protein level).
CC       {ECO:0000269|PubMed:11506704}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAD32473.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AF484555; AAM18673.1; -; mRNA.
DR   EMBL; AK173195; BAD32473.1; ALT_INIT; mRNA.
DR   CCDS; CCDS48582.1; -.
DR   RefSeq; NP_081701.2; NM_027425.3.
DR   AlphaFoldDB; Q8R4C2; -.
DR   SMR; Q8R4C2; -.
DR   BioGRID; 214046; 1.
DR   STRING; 10090.ENSMUSP00000113429; -.
DR   iPTMnet; Q8R4C2; -.
DR   PhosphoSitePlus; Q8R4C2; -.
DR   MaxQB; Q8R4C2; -.
DR   PaxDb; 10090-ENSMUSP00000113429; -.
DR   ProteomicsDB; 256808; -.
DR   Pumba; Q8R4C2; -.
DR   Antibodypedia; 45205; 93 antibodies from 20 providers.
DR   DNASU; 70432; -.
DR   Ensembl; ENSMUST00000119567.8; ENSMUSP00000113429.2; ENSMUSG00000020070.19.
DR   GeneID; 70432; -.
DR   KEGG; mmu:70432; -.
DR   UCSC; uc007fjn.1; mouse.
DR   AGR; MGI:1917682; -.
DR   CTD; 55680; -.
DR   MGI; MGI:1917682; Rufy2.
DR   VEuPathDB; HostDB:ENSMUSG00000020070; -.
DR   eggNOG; KOG4381; Eukaryota.
DR   GeneTree; ENSGT00940000155595; -.
DR   HOGENOM; CLU_014576_3_1_1; -.
DR   InParanoid; Q8R4C2; -.
DR   OMA; KNDMISR; -.
DR   OrthoDB; 5385125at2759; -.
DR   PhylomeDB; Q8R4C2; -.
DR   TreeFam; TF323904; -.
DR   BioGRID-ORCS; 70432; 0 hits in 77 CRISPR screens.
DR   ChiTaRS; Rufy2; mouse.
DR   PRO; PR:Q8R4C2; -.
DR   Proteomes; UP000000589; Chromosome 10.
DR   RNAct; Q8R4C2; Protein.
DR   Bgee; ENSMUSG00000020070; Expressed in rostral migratory stream and 225 other cell types or tissues.
DR   ExpressionAtlas; Q8R4C2; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0017124; F:SH3 domain binding; ISO:MGI.
DR   CDD; cd15759; FYVE_RUFY2; 1.
DR   CDD; cd17695; RUN_RUFY2; 1.
DR   Gene3D; 1.20.5.170; -; 1.
DR   Gene3D; 1.20.58.900; -; 1.
DR   Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR   InterPro; IPR047333; FYVE_RUFY2.
DR   InterPro; IPR047335; RUFY1-3.
DR   InterPro; IPR004012; Run_dom.
DR   InterPro; IPR037213; Run_dom_sf.
DR   InterPro; IPR047332; RUN_RUFY2.
DR   InterPro; IPR000306; Znf_FYVE.
DR   InterPro; IPR017455; Znf_FYVE-rel.
DR   InterPro; IPR011011; Znf_FYVE_PHD.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   PANTHER; PTHR45956:SF3; RUN AND FYVE DOMAIN-CONTAINING PROTEIN 2; 1.
DR   PANTHER; PTHR45956; RUN AND FYVE DOMAIN-CONTAINING PROTEIN 2-LIKE PROTEIN; 1.
DR   Pfam; PF01363; FYVE; 1.
DR   Pfam; PF02759; RUN; 1.
DR   SMART; SM00064; FYVE; 1.
DR   SMART; SM00593; RUN; 1.
DR   SUPFAM; SSF57903; FYVE/PHD zinc finger; 1.
DR   SUPFAM; SSF140741; RUN domain-like; 1.
DR   PROSITE; PS50826; RUN; 1.
DR   PROSITE; PS50178; ZF_FYVE; 1.
DR   Genevisible; Q8R4C2; MM.
PE   1: Evidence at protein level;
KW   Coiled coil; Metal-binding; Nucleus; Reference proteome; Zinc; Zinc-finger.
FT   CHAIN           1..606
FT                   /note="RUN and FYVE domain-containing protein 2"
FT                   /id="PRO_0000245831"
FT   DOMAIN          37..169
FT                   /note="RUN"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00178"
FT   ZN_FING         540..598
FT                   /note="FYVE-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00091"
FT   COILED          210..267
FT                   /evidence="ECO:0000255"
FT   COILED          294..515
FT                   /evidence="ECO:0000255"
FT   BINDING         546
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00091"
FT   BINDING         549
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00091"
FT   BINDING         562
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00091"
FT   BINDING         565
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00091"
FT   BINDING         570
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00091"
FT   BINDING         573
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00091"
FT   BINDING         590
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00091"
FT   BINDING         593
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00091"
FT   CONFLICT        208
FT                   /note="Y -> C (in Ref. 2; AAM18673)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        435
FT                   /note="I -> V (in Ref. 2; AAM18673)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   606 AA;  70093 MW;  741EE7EA1E0A4EF2 CRC64;
     MATKDPTAVE RANLLNMAKL SIKGLIESAL SFGRTLDSDY PPLQQFFVVM EHCLKHGLKG
     RKSFLSYNKT IWGPLELVEK LYPEAEEIGA SVRDLPGLKT PLGRARAWLR LALMQKKMAD
     YLRCLIIQRE LLSEFYEYHA LMMEEEGAVI VGLLVGLNVI DANLCVKGED LDSQVGVIDF
     SMYLKNEEEI GNKERNVQIA AILDQKNYVE ELNRQLNSTV SSLHSRVDSL EKSNTKLIEE
     LAIAKNNIIK LQEENHQLRS ENELILMRTR QHLEVTKVDV ETELQTYKHS RQGLDEMYND
     ARRQLRDESQ LRQDVENELS VQVGMKHEME LAMKLLEKDI HEKQDTLIGL RQQLEEVKAI
     NIEMYQRLQG SEDGLKEKNE IIARLEEKTN KITTAMRQLE QRLQQAEKAQ KEAEAEDEKY
     AQECLSQSDS LQRQISQKEQ QLVQLETDLK IEKEWRQTLQ EDLQKEKDVL SHLRHETQKV
     ISLKKEFLNL QDENQQLKRI YQEQEQALQE LGSKLCESKL KIDDIKEANK ALQGLVWLKD
     KDATHCKLCE KEFSLSKRKH HCRNCGEIFC NACSDNELPL PSSPKPVRVC DSCHAMLIQR
     CSSNMP
//