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Q8R493

- ACHB4_MOUSE

UniProt

Q8R493 - ACHB4_MOUSE

Protein

Neuronal acetylcholine receptor subunit beta-4

Gene

Chrnb4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.By similarity

    GO - Molecular functioni

    1. acetylcholine-activated cation-selective channel activity Source: InterPro
    2. acetylcholine receptor activity Source: Ensembl

    GO - Biological processi

    1. action potential Source: MGI
    2. behavioral response to nicotine Source: MGI
    3. locomotory behavior Source: MGI
    4. regulation of membrane potential Source: MGI
    5. regulation of smooth muscle contraction Source: MGI
    6. response to nicotine Source: MGI
    7. smooth muscle contraction Source: MGI
    8. synaptic transmission involved in micturition Source: MGI

    Keywords - Molecular functioni

    Ion channel, Ligand-gated ion channel, Receptor

    Keywords - Biological processi

    Ion transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_198583. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
    REACT_198585. Highly calcium permeable nicotinic acetylcholine receptors.
    REACT_198593. Highly sodium permeable acetylcholine nicotinic receptors.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neuronal acetylcholine receptor subunit beta-4
    Gene namesi
    Name:Chrnb4
    Synonyms:Acrb4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:87892. Chrnb4.

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: MGI
    4. postsynaptic membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 495475Neuronal acetylcholine receptor subunit beta-4PRO_0000000390Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi35 – 351N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi92 – 921N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi137 – 1371N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi152 ↔ 166By similarity
    Glycosylationi165 – 1651N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ8R493.
    PRIDEiQ8R493.

    PTM databases

    PhosphoSiteiQ8R493.

    Expressioni

    Tissue specificityi

    Predominantly expressed by immature T-cells in the thymus.1 Publication

    Gene expression databases

    ArrayExpressiQ8R493.
    BgeeiQ8R493.
    GenevestigatoriQ8R493.

    Interactioni

    Subunit structurei

    Neuronal AChR is composed of two different types of subunits: alpha and beta. Beta-4 subunit can be combined to alpha-2, alpha-3 or alpha-4 to give rise to functional receptors. Interacts with RIC3; which is required for proper folding and assembly By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ8R493.
    SMRiQ8R493. Positions 24-327, 456-487.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini21 – 235215ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini257 – 2648CytoplasmicSequence Analysis
    Topological domaini286 – 29712ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini319 – 463145CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini485 – 49511ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei236 – 25621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei265 – 28521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei298 – 31821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei464 – 48421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG290206.
    GeneTreeiENSGT00750000117277.
    HOGENOMiHOG000006757.
    HOVERGENiHBG003756.
    InParanoidiQ8R493.
    KOiK04815.
    OMAiMTPTASM.
    OrthoDBiEOG72JWGV.
    PhylomeDBiQ8R493.
    TreeFamiTF315605.

    Family and domain databases

    Gene3Di1.20.120.370. 2 hits.
    2.70.170.10. 1 hit.
    InterProiIPR027361. Acetylcholine_rcpt_TM.
    IPR006202. Neur_chan_lig-bd.
    IPR006201. Neur_channel.
    IPR006029. Neurotrans-gated_channel_TM.
    IPR018000. Neurotransmitter_ion_chnl_CS.
    IPR002394. Nicotinic_acetylcholine_rcpt.
    [Graphical view]
    PANTHERiPTHR18945. PTHR18945. 1 hit.
    PfamiPF02931. Neur_chan_LBD. 1 hit.
    PF02932. Neur_chan_memb. 1 hit.
    [Graphical view]
    PRINTSiPR00254. NICOTINICR.
    PR00252. NRIONCHANNEL.
    SUPFAMiSSF63712. SSF63712. 1 hit.
    SSF90112. SSF90112. 1 hit.
    TIGRFAMsiTIGR00860. LIC. 1 hit.
    PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8R493-1 [UniParc]FASTAAdd to Basket

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    MRGTPLLLVS LFALLQPGDC RLANAEEKLM DDLLNKTRYN NLIRPATSSS    50
    QLISIRLELS LSQLISVNER EQIMTTSIWL KQEWTDYRLA WNSSCYEGVN 100
    ILRIPAKRVW LPDIVLYNNA DGTYEVSVYT NVIVRSNGSI QWLPPAIYKS 150
    ACKIEVKHFP FDQQNCTLKF RSWTYDHTEI DMVLKSPTAI MDDFTPSGEW 200
    DIVALPGRRT VNPQDPSYVD VTYDFIIKRK PLFYTINLII PCVLITSLAI 250
    LVFYLPSDCG EKMTLCISVL LALTFFLLLI SKIVPPTSLD IPLIGKYLLF 300
    TMVLVTFSIV TTVCVLNVHH RSPSTHTMAS WVKECFLHKL PTFLFMKRPG 350
    LEVSPARVPH SSQLHLTTAE ATSTSALGPS SPSNLYGNSM YFVNPVPATP 400
    KSAVSSHTAG LPRDARLRSS GRFRQDLQEA LEGVSFIAQH LESDDRDQSV 450
    IEDWKFVAMV VDRLFLWVFV IVCILGTMGL FLPPLFQIHA PSKGL 495
    Length:495
    Mass (Da):55,809
    Last modified:June 1, 2002 - v1
    Checksum:i025AF3585C17A6A8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF492840 mRNA. Translation: AAM11659.1.
    AY574269 mRNA. Translation: AAS90365.1.
    AF325351 mRNA. Translation: AAL37367.1.
    CCDSiCCDS23200.1.
    RefSeqiNP_683746.1. NM_148944.4.
    UniGeneiMm.32694.

    Genome annotation databases

    EnsembliENSMUST00000034854; ENSMUSP00000034854; ENSMUSG00000035200.
    GeneIDi108015.
    KEGGimmu:108015.
    UCSCiuc009prz.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF492840 mRNA. Translation: AAM11659.1 .
    AY574269 mRNA. Translation: AAS90365.1 .
    AF325351 mRNA. Translation: AAL37367.1 .
    CCDSi CCDS23200.1.
    RefSeqi NP_683746.1. NM_148944.4.
    UniGenei Mm.32694.

    3D structure databases

    ProteinModelPortali Q8R493.
    SMRi Q8R493. Positions 24-327, 456-487.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi Q8R493.
    GuidetoPHARMACOLOGYi 474.

    PTM databases

    PhosphoSitei Q8R493.

    Proteomic databases

    PaxDbi Q8R493.
    PRIDEi Q8R493.

    Protocols and materials databases

    DNASUi 108015.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034854 ; ENSMUSP00000034854 ; ENSMUSG00000035200 .
    GeneIDi 108015.
    KEGGi mmu:108015.
    UCSCi uc009prz.1. mouse.

    Organism-specific databases

    CTDi 1143.
    MGIi MGI:87892. Chrnb4.

    Phylogenomic databases

    eggNOGi NOG290206.
    GeneTreei ENSGT00750000117277.
    HOGENOMi HOG000006757.
    HOVERGENi HBG003756.
    InParanoidi Q8R493.
    KOi K04815.
    OMAi MTPTASM.
    OrthoDBi EOG72JWGV.
    PhylomeDBi Q8R493.
    TreeFami TF315605.

    Enzyme and pathway databases

    Reactomei REACT_198583. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
    REACT_198585. Highly calcium permeable nicotinic acetylcholine receptors.
    REACT_198593. Highly sodium permeable acetylcholine nicotinic receptors.

    Miscellaneous databases

    NextBioi 359889.
    PROi Q8R493.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8R493.
    Bgeei Q8R493.
    Genevestigatori Q8R493.

    Family and domain databases

    Gene3Di 1.20.120.370. 2 hits.
    2.70.170.10. 1 hit.
    InterProi IPR027361. Acetylcholine_rcpt_TM.
    IPR006202. Neur_chan_lig-bd.
    IPR006201. Neur_channel.
    IPR006029. Neurotrans-gated_channel_TM.
    IPR018000. Neurotransmitter_ion_chnl_CS.
    IPR002394. Nicotinic_acetylcholine_rcpt.
    [Graphical view ]
    PANTHERi PTHR18945. PTHR18945. 1 hit.
    Pfami PF02931. Neur_chan_LBD. 1 hit.
    PF02932. Neur_chan_memb. 1 hit.
    [Graphical view ]
    PRINTSi PR00254. NICOTINICR.
    PR00252. NRIONCHANNEL.
    SUPFAMi SSF63712. SSF63712. 1 hit.
    SSF90112. SSF90112. 1 hit.
    TIGRFAMsi TIGR00860. LIC. 1 hit.
    PROSITEi PS00236. NEUROTR_ION_CHANNEL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a mouse nicotinic acetylcholine receptor beta 4 subunit cDNA."
      Lautner M.A., Remias J., Curtis C., Bhandarkar S., Stitzel J.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C3H.
    2. Groot Kormelink P.J.
      Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Brain.
    3. "Differential expression of nicotinic acetylcholine receptor subunits in fetal and neonatal mouse thymus."
      Kuo Y.-P., Lucero L., Michaels J., DeLuca D., Lukas R.L.
      J. Neuroimmunol. 130:140-154(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 120-395, TISSUE SPECIFICITY.
      Strain: C57BL/6.
      Tissue: Thymus.

    Entry informationi

    Entry nameiACHB4_MOUSE
    AccessioniPrimary (citable) accession number: Q8R493
    Secondary accession number(s): Q8VI06
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 4, 2005
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3