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Protein

Neuronal acetylcholine receptor subunit beta-4

Gene

Chrnb4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.By similarity

GO - Molecular functioni

GO - Biological processi

  • action potential Source: MGI
  • behavioral response to nicotine Source: MGI
  • cation transmembrane transport Source: GO_Central
  • locomotory behavior Source: MGI
  • positive regulation of transmission of nerve impulse Source: CACAO
  • protein heterooligomerization Source: Ensembl
  • regulation of membrane potential Source: MGI
  • regulation of smooth muscle contraction Source: MGI
  • response to nicotine Source: MGI
  • signal transduction Source: MGI
  • smooth muscle contraction Source: MGI
  • synaptic transmission, cholinergic Source: GO_Central
  • synaptic transmission involved in micturition Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-629587. Highly sodium permeable acetylcholine nicotinic receptors.
R-MMU-629594. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
R-MMU-629597. Highly calcium permeable nicotinic acetylcholine receptors.
R-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit beta-4
Gene namesi
Name:Chrnb4
Synonyms:Acrb4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:87892. Chrnb4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 235ExtracellularSequence analysisAdd BLAST215
Transmembranei236 – 256HelicalSequence analysisAdd BLAST21
Topological domaini257 – 264CytoplasmicSequence analysis8
Transmembranei265 – 285HelicalSequence analysisAdd BLAST21
Topological domaini286 – 297ExtracellularSequence analysisAdd BLAST12
Transmembranei298 – 318HelicalSequence analysisAdd BLAST21
Topological domaini319 – 463CytoplasmicSequence analysisAdd BLAST145
Transmembranei464 – 484HelicalSequence analysisAdd BLAST21
Topological domaini485 – 495ExtracellularSequence analysisAdd BLAST11

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3301382.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000000039021 – 495Neuronal acetylcholine receptor subunit beta-4Add BLAST475

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi35N-linked (GlcNAc...)Sequence analysis1
Glycosylationi92N-linked (GlcNAc...)Sequence analysis1
Glycosylationi137N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi152 ↔ 166By similarity
Glycosylationi165N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8R493.
PRIDEiQ8R493.

PTM databases

PhosphoSitePlusiQ8R493.

Expressioni

Tissue specificityi

Predominantly expressed by immature T-cells in the thymus.1 Publication

Gene expression databases

BgeeiENSMUSG00000035200.
ExpressionAtlasiQ8R493. baseline and differential.
GenevisibleiQ8R493. MM.

Interactioni

Subunit structurei

Neuronal AChR is composed of two different types of subunits: alpha and beta. Beta-4 subunit can be combined to alpha-2, alpha-3 or alpha-4 to give rise to functional receptors. Interacts with RIC3; which is required for proper folding and assembly (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034854.

Chemistry databases

BindingDBiQ8R493.

Structurei

3D structure databases

ProteinModelPortaliQ8R493.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
GeneTreeiENSGT00760000118930.
HOGENOMiHOG000006757.
HOVERGENiHBG003756.
InParanoidiQ8R493.
KOiK04815.
OMAiFMKRPGP.
OrthoDBiEOG091G0R20.
PhylomeDBiQ8R493.
TreeFamiTF315605.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R493-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGTPLLLVS LFALLQPGDC RLANAEEKLM DDLLNKTRYN NLIRPATSSS
60 70 80 90 100
QLISIRLELS LSQLISVNER EQIMTTSIWL KQEWTDYRLA WNSSCYEGVN
110 120 130 140 150
ILRIPAKRVW LPDIVLYNNA DGTYEVSVYT NVIVRSNGSI QWLPPAIYKS
160 170 180 190 200
ACKIEVKHFP FDQQNCTLKF RSWTYDHTEI DMVLKSPTAI MDDFTPSGEW
210 220 230 240 250
DIVALPGRRT VNPQDPSYVD VTYDFIIKRK PLFYTINLII PCVLITSLAI
260 270 280 290 300
LVFYLPSDCG EKMTLCISVL LALTFFLLLI SKIVPPTSLD IPLIGKYLLF
310 320 330 340 350
TMVLVTFSIV TTVCVLNVHH RSPSTHTMAS WVKECFLHKL PTFLFMKRPG
360 370 380 390 400
LEVSPARVPH SSQLHLTTAE ATSTSALGPS SPSNLYGNSM YFVNPVPATP
410 420 430 440 450
KSAVSSHTAG LPRDARLRSS GRFRQDLQEA LEGVSFIAQH LESDDRDQSV
460 470 480 490
IEDWKFVAMV VDRLFLWVFV IVCILGTMGL FLPPLFQIHA PSKGL
Length:495
Mass (Da):55,809
Last modified:June 1, 2002 - v1
Checksum:i025AF3585C17A6A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492840 mRNA. Translation: AAM11659.1.
AY574269 mRNA. Translation: AAS90365.1.
AF325351 mRNA. Translation: AAL37367.1.
CCDSiCCDS23200.1.
RefSeqiNP_683746.1. NM_148944.4.
UniGeneiMm.32694.

Genome annotation databases

EnsembliENSMUST00000034854; ENSMUSP00000034854; ENSMUSG00000035200.
GeneIDi108015.
KEGGimmu:108015.
UCSCiuc009prz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492840 mRNA. Translation: AAM11659.1.
AY574269 mRNA. Translation: AAS90365.1.
AF325351 mRNA. Translation: AAL37367.1.
CCDSiCCDS23200.1.
RefSeqiNP_683746.1. NM_148944.4.
UniGeneiMm.32694.

3D structure databases

ProteinModelPortaliQ8R493.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034854.

Chemistry databases

BindingDBiQ8R493.
ChEMBLiCHEMBL3301382.

PTM databases

PhosphoSitePlusiQ8R493.

Proteomic databases

PaxDbiQ8R493.
PRIDEiQ8R493.

Protocols and materials databases

DNASUi108015.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034854; ENSMUSP00000034854; ENSMUSG00000035200.
GeneIDi108015.
KEGGimmu:108015.
UCSCiuc009prz.1. mouse.

Organism-specific databases

CTDi1143.
MGIiMGI:87892. Chrnb4.

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
GeneTreeiENSGT00760000118930.
HOGENOMiHOG000006757.
HOVERGENiHBG003756.
InParanoidiQ8R493.
KOiK04815.
OMAiFMKRPGP.
OrthoDBiEOG091G0R20.
PhylomeDBiQ8R493.
TreeFamiTF315605.

Enzyme and pathway databases

ReactomeiR-MMU-629587. Highly sodium permeable acetylcholine nicotinic receptors.
R-MMU-629594. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
R-MMU-629597. Highly calcium permeable nicotinic acetylcholine receptors.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ8R493.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035200.
ExpressionAtlasiQ8R493. baseline and differential.
GenevisibleiQ8R493. MM.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACHB4_MOUSE
AccessioniPrimary (citable) accession number: Q8R493
Secondary accession number(s): Q8VI06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: June 1, 2002
Last modified: November 30, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.