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Protein

Neuronal acetylcholine receptor subunit beta-4

Gene

Chrnb4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.By similarity

GO - Molecular functioni

  1. acetylcholine-activated cation-selective channel activity Source: InterPro
  2. acetylcholine receptor activity Source: MGI

GO - Biological processi

  1. action potential Source: MGI
  2. behavioral response to nicotine Source: MGI
  3. locomotory behavior Source: MGI
  4. positive regulation of transmission of nerve impulse Source: CACAO
  5. regulation of membrane potential Source: MGI
  6. regulation of smooth muscle contraction Source: MGI
  7. response to nicotine Source: MGI
  8. signal transduction Source: MGI
  9. smooth muscle contraction Source: MGI
  10. synaptic transmission involved in micturition Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiREACT_291046. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
REACT_305614. Highly sodium permeable acetylcholine nicotinic receptors.
REACT_329749. Highly calcium permeable nicotinic acetylcholine receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit beta-4
Gene namesi
Name:Chrnb4
Synonyms:Acrb4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:87892. Chrnb4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 235215ExtracellularSequence AnalysisAdd
BLAST
Transmembranei236 – 25621HelicalSequence AnalysisAdd
BLAST
Topological domaini257 – 2648CytoplasmicSequence Analysis
Transmembranei265 – 28521HelicalSequence AnalysisAdd
BLAST
Topological domaini286 – 29712ExtracellularSequence AnalysisAdd
BLAST
Transmembranei298 – 31821HelicalSequence AnalysisAdd
BLAST
Topological domaini319 – 463145CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei464 – 48421HelicalSequence AnalysisAdd
BLAST
Topological domaini485 – 49511ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. acetylcholine-gated channel complex Source: MGI
  2. cell junction Source: UniProtKB-KW
  3. plasma membrane Source: MGI
  4. postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 495475Neuronal acetylcholine receptor subunit beta-4PRO_0000000390Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi35 – 351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi92 – 921N-linked (GlcNAc...)Sequence Analysis
Glycosylationi137 – 1371N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi152 ↔ 166By similarity
Glycosylationi165 – 1651N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8R493.
PRIDEiQ8R493.

PTM databases

PhosphoSiteiQ8R493.

Expressioni

Tissue specificityi

Predominantly expressed by immature T-cells in the thymus.1 Publication

Gene expression databases

BgeeiQ8R493.
ExpressionAtlasiQ8R493. baseline and differential.
GenevestigatoriQ8R493.

Interactioni

Subunit structurei

Neuronal AChR is composed of two different types of subunits: alpha and beta. Beta-4 subunit can be combined to alpha-2, alpha-3 or alpha-4 to give rise to functional receptors. Interacts with RIC3; which is required for proper folding and assembly (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ8R493.
SMRiQ8R493. Positions 24-327, 456-487.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG290206.
GeneTreeiENSGT00760000118930.
HOGENOMiHOG000006757.
HOVERGENiHBG003756.
InParanoidiQ8R493.
KOiK04815.
OMAiMTPTASM.
OrthoDBiEOG72JWGV.
PhylomeDBiQ8R493.
TreeFamiTF315605.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R493-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGTPLLLVS LFALLQPGDC RLANAEEKLM DDLLNKTRYN NLIRPATSSS
60 70 80 90 100
QLISIRLELS LSQLISVNER EQIMTTSIWL KQEWTDYRLA WNSSCYEGVN
110 120 130 140 150
ILRIPAKRVW LPDIVLYNNA DGTYEVSVYT NVIVRSNGSI QWLPPAIYKS
160 170 180 190 200
ACKIEVKHFP FDQQNCTLKF RSWTYDHTEI DMVLKSPTAI MDDFTPSGEW
210 220 230 240 250
DIVALPGRRT VNPQDPSYVD VTYDFIIKRK PLFYTINLII PCVLITSLAI
260 270 280 290 300
LVFYLPSDCG EKMTLCISVL LALTFFLLLI SKIVPPTSLD IPLIGKYLLF
310 320 330 340 350
TMVLVTFSIV TTVCVLNVHH RSPSTHTMAS WVKECFLHKL PTFLFMKRPG
360 370 380 390 400
LEVSPARVPH SSQLHLTTAE ATSTSALGPS SPSNLYGNSM YFVNPVPATP
410 420 430 440 450
KSAVSSHTAG LPRDARLRSS GRFRQDLQEA LEGVSFIAQH LESDDRDQSV
460 470 480 490
IEDWKFVAMV VDRLFLWVFV IVCILGTMGL FLPPLFQIHA PSKGL
Length:495
Mass (Da):55,809
Last modified:June 1, 2002 - v1
Checksum:i025AF3585C17A6A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492840 mRNA. Translation: AAM11659.1.
AY574269 mRNA. Translation: AAS90365.1.
AF325351 mRNA. Translation: AAL37367.1.
CCDSiCCDS23200.1.
RefSeqiNP_683746.1. NM_148944.4.
UniGeneiMm.32694.

Genome annotation databases

EnsembliENSMUST00000034854; ENSMUSP00000034854; ENSMUSG00000035200.
GeneIDi108015.
KEGGimmu:108015.
UCSCiuc009prz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492840 mRNA. Translation: AAM11659.1.
AY574269 mRNA. Translation: AAS90365.1.
AF325351 mRNA. Translation: AAL37367.1.
CCDSiCCDS23200.1.
RefSeqiNP_683746.1. NM_148944.4.
UniGeneiMm.32694.

3D structure databases

ProteinModelPortaliQ8R493.
SMRiQ8R493. Positions 24-327, 456-487.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

ChEMBLiCHEMBL3301382.
GuidetoPHARMACOLOGYi474.

PTM databases

PhosphoSiteiQ8R493.

Proteomic databases

PaxDbiQ8R493.
PRIDEiQ8R493.

Protocols and materials databases

DNASUi108015.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034854; ENSMUSP00000034854; ENSMUSG00000035200.
GeneIDi108015.
KEGGimmu:108015.
UCSCiuc009prz.1. mouse.

Organism-specific databases

CTDi1143.
MGIiMGI:87892. Chrnb4.

Phylogenomic databases

eggNOGiNOG290206.
GeneTreeiENSGT00760000118930.
HOGENOMiHOG000006757.
HOVERGENiHBG003756.
InParanoidiQ8R493.
KOiK04815.
OMAiMTPTASM.
OrthoDBiEOG72JWGV.
PhylomeDBiQ8R493.
TreeFamiTF315605.

Enzyme and pathway databases

ReactomeiREACT_291046. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
REACT_305614. Highly sodium permeable acetylcholine nicotinic receptors.
REACT_329749. Highly calcium permeable nicotinic acetylcholine receptors.

Miscellaneous databases

NextBioi359889.
PROiQ8R493.
SOURCEiSearch...

Gene expression databases

BgeeiQ8R493.
ExpressionAtlasiQ8R493. baseline and differential.
GenevestigatoriQ8R493.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning of a mouse nicotinic acetylcholine receptor beta 4 subunit cDNA."
    Lautner M.A., Remias J., Curtis C., Bhandarkar S., Stitzel J.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C3H.
  2. Groot Kormelink P.J.
    Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Brain.
  3. "Differential expression of nicotinic acetylcholine receptor subunits in fetal and neonatal mouse thymus."
    Kuo Y.-P., Lucero L., Michaels J., DeLuca D., Lukas R.L.
    J. Neuroimmunol. 130:140-154(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 120-395, TISSUE SPECIFICITY.
    Strain: C57BL/6.
    Tissue: Thymus.

Entry informationi

Entry nameiACHB4_MOUSE
AccessioniPrimary (citable) accession number: Q8R493
Secondary accession number(s): Q8VI06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: June 1, 2002
Last modified: April 29, 2015
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.