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Q8R493 (ACHB4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neuronal acetylcholine receptor subunit beta-4
Gene names
Name:Chrnb4
Synonyms:Acrb4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length495 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane By similarity.

Subunit structure

Neuronal AChR is composed of two different types of subunits: alpha and beta. Beta-4 subunit can be combined to alpha-2, alpha-3 or alpha-4 to give rise to functional receptors. Interacts with RIC3; which is required for proper folding and assembly By similarity.

Subcellular location

Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein By similarity. Cell membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Predominantly expressed by immature T-cells in the thymus. Ref.3

Sequence similarities

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-4/CHRNB4 sub-subfamily. [View classification]

Ontologies

Keywords
   Biological processIon transport
Transport
   Cellular componentCell junction
Cell membrane
Membrane
Postsynaptic cell membrane
Synapse
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionIon channel
Ligand-gated ion channel
Receptor
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaction potential

Inferred from genetic interaction PubMed 10531434. Source: MGI

behavioral response to nicotine

Inferred from mutant phenotype PubMed 15275829PubMed 15537871. Source: MGI

locomotory behavior

Inferred from mutant phenotype PubMed 15275829. Source: MGI

regulation of membrane potential

Inferred from genetic interaction PubMed 10531434. Source: MGI

regulation of smooth muscle contraction

Inferred from mutant phenotype PubMed 10771006. Source: MGI

response to nicotine

Inferred from genetic interaction PubMed 10531434. Source: MGI

smooth muscle contraction

Inferred from genetic interaction PubMed 10531434. Source: MGI

synaptic transmission involved in micturition

Inferred from mutant phenotype PubMed 10771006. Source: MGI

   Cellular_componentcell junction

Inferred from electronic annotation. Source: UniProtKB-KW

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from sequence orthology PubMed 19474315. Source: MGI

postsynaptic membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionacetylcholine receptor activity

Inferred from electronic annotation. Source: Ensembl

acetylcholine-activated cation-selective channel activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 495475Neuronal acetylcholine receptor subunit beta-4
PRO_0000000390

Regions

Topological domain21 – 235215Extracellular Potential
Transmembrane236 – 25621Helical; Potential
Topological domain257 – 2648Cytoplasmic Potential
Transmembrane265 – 28521Helical; Potential
Topological domain286 – 29712Extracellular Potential
Transmembrane298 – 31821Helical; Potential
Topological domain319 – 463145Cytoplasmic Potential
Transmembrane464 – 48421Helical; Potential
Topological domain485 – 49511Extracellular Potential

Amino acid modifications

Glycosylation351N-linked (GlcNAc...) Potential
Glycosylation921N-linked (GlcNAc...) Potential
Glycosylation1371N-linked (GlcNAc...) Potential
Glycosylation1651N-linked (GlcNAc...) Potential
Disulfide bond152 ↔ 166 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8R493 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 025AF3585C17A6A8

FASTA49555,809
        10         20         30         40         50         60 
MRGTPLLLVS LFALLQPGDC RLANAEEKLM DDLLNKTRYN NLIRPATSSS QLISIRLELS 

        70         80         90        100        110        120 
LSQLISVNER EQIMTTSIWL KQEWTDYRLA WNSSCYEGVN ILRIPAKRVW LPDIVLYNNA 

       130        140        150        160        170        180 
DGTYEVSVYT NVIVRSNGSI QWLPPAIYKS ACKIEVKHFP FDQQNCTLKF RSWTYDHTEI 

       190        200        210        220        230        240 
DMVLKSPTAI MDDFTPSGEW DIVALPGRRT VNPQDPSYVD VTYDFIIKRK PLFYTINLII 

       250        260        270        280        290        300 
PCVLITSLAI LVFYLPSDCG EKMTLCISVL LALTFFLLLI SKIVPPTSLD IPLIGKYLLF 

       310        320        330        340        350        360 
TMVLVTFSIV TTVCVLNVHH RSPSTHTMAS WVKECFLHKL PTFLFMKRPG LEVSPARVPH 

       370        380        390        400        410        420 
SSQLHLTTAE ATSTSALGPS SPSNLYGNSM YFVNPVPATP KSAVSSHTAG LPRDARLRSS 

       430        440        450        460        470        480 
GRFRQDLQEA LEGVSFIAQH LESDDRDQSV IEDWKFVAMV VDRLFLWVFV IVCILGTMGL 

       490 
FLPPLFQIHA PSKGL 

« Hide

References

[1]"Cloning of a mouse nicotinic acetylcholine receptor beta 4 subunit cDNA."
Lautner M.A., Remias J., Curtis C., Bhandarkar S., Stitzel J.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C3H.
[2]Groot Kormelink P.J.
Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Brain.
[3]"Differential expression of nicotinic acetylcholine receptor subunits in fetal and neonatal mouse thymus."
Kuo Y.-P., Lucero L., Michaels J., DeLuca D., Lukas R.L.
J. Neuroimmunol. 130:140-154(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 120-395, TISSUE SPECIFICITY.
Strain: C57BL/6.
Tissue: Thymus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF492840 mRNA. Translation: AAM11659.1.
AY574269 mRNA. Translation: AAS90365.1.
AF325351 mRNA. Translation: AAL37367.1.
RefSeqNP_683746.1. NM_148944.4.
UniGeneMm.32694.

3D structure databases

ProteinModelPortalQ8R493.
SMRQ8R493. Positions 23-492.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBQ8R493.

PTM databases

PhosphoSiteQ8R493.

Proteomic databases

PaxDbQ8R493.
PRIDEQ8R493.

Protocols and materials databases

DNASU108015.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034854; ENSMUSP00000034854; ENSMUSG00000035200.
GeneID108015.
KEGGmmu:108015.
UCSCuc009prz.1. mouse.

Organism-specific databases

CTD1143.
MGIMGI:87892. Chrnb4.

Phylogenomic databases

eggNOGNOG290206.
GeneTreeENSGT00750000117277.
HOGENOMHOG000006757.
HOVERGENHBG003756.
InParanoidQ8R493.
KOK04815.
OMAMTPTASM.
OrthoDBEOG72JWGV.
PhylomeDBQ8R493.
TreeFamTF315605.

Gene expression databases

ArrayExpressQ8R493.
BgeeQ8R493.
GenevestigatorQ8R493.

Family and domain databases

Gene3D1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERPTHR18945. PTHR18945. 1 hit.
PfamPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsTIGR00860. LIC. 1 hit.
PROSITEPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio359889.
PROQ8R493.
SOURCESearch...

Entry information

Entry nameACHB4_MOUSE
AccessionPrimary (citable) accession number: Q8R493
Secondary accession number(s): Q8VI06
Entry history
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: June 1, 2002
Last modified: April 16, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot