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Protein

Transient receptor potential cation channel subfamily M member 8

Gene

Trpm8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel
Biological processIon transport, Sensory transduction, Transport

Enzyme and pathway databases

ReactomeiR-RNO-3295583 TRP channels

Names & Taxonomyi

Protein namesi
Recommended name:
Transient receptor potential cation channel subfamily M member 8
Alternative name(s):
Cold menthol receptor 1
Gene namesi
Name:Trpm8
Synonyms:Cmr1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi

Organism-specific databases

RGDi620762 Trpm8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 691CytoplasmicSequence analysisAdd BLAST691
Transmembranei692 – 712Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini713 – 734ExtracellularSequence analysisAdd BLAST22
Transmembranei735 – 755Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini756 – 759CytoplasmicSequence analysis4
Transmembranei760 – 780Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini781 – 793ExtracellularSequence analysisAdd BLAST13
Transmembranei794 – 814Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini815 – 829CytoplasmicSequence analysisAdd BLAST15
Transmembranei830 – 850Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini851 – 958ExtracellularSequence analysisAdd BLAST108
Transmembranei959 – 979Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini980 – 1104CytoplasmicSequence analysisAdd BLAST125

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5011
GuidetoPHARMACOLOGYi500

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002153351 – 1104Transient receptor potential cation channel subfamily M member 8Add BLAST1104

Proteomic databases

PaxDbiQ8R455
PRIDEiQ8R455

PTM databases

PhosphoSitePlusiQ8R455

Expressioni

Tissue specificityi

Expressed in dorsal root and trigeminal ganglia. Specifically expressed in a subset of sensory neurons, including cold-sensitive neurons in trigeminal neurons.2 Publications

Gene expression databases

BgeeiENSRNOG00000019035
GenevisibleiQ8R455 RN

Interactioni

Subunit structurei

Interacts (via N-terminus and C-terminus domains) with TCAF1; the interaction stimulates TRPM8 channel activity. Interacts (via N-terminus and C-terminus domains) with TCAF2; the interaction inhibits TRPM8 channel activity (By similarity). Homotetramer.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GNAQP501484EBI-15993527,EBI-3909604From Homo sapiens.

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-61851N
IntActiQ8R455, 1 interactor
STRINGi10116.ENSRNOP00000025879

Chemistry databases

BindingDBiQ8R455

Structurei

3D structure databases

ProteinModelPortaliQ8R455
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1067 – 1104Sequence analysisAdd BLAST38

Domaini

The coiled coil region is required for multimerization.By similarity

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410KCTC Eukaryota
ENOG4110YBY LUCA
GeneTreeiENSGT00760000119127
HOGENOMiHOG000236350
HOVERGENiHBG100888
InParanoidiQ8R455
KOiK04983
OMAiETLAWEG
OrthoDBiEOG091G035A
PhylomeDBiQ8R455
TreeFamiTF314204

Family and domain databases

InterProiView protein in InterPro
IPR029603 TRPM8
PANTHERiPTHR13800:SF9 PTHR13800:SF9, 1 hit

Sequencei

Sequence statusi: Complete.

Q8R455-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFEGARLSM RSRRNGTLGS TRTLYSSVSR STDVSYSESD LVNFIQANFK
60 70 80 90 100
KRECVFFTRD SKAMESICKC GYAQSQHIEG TQINQNEKWN YKKHTKEFPT
110 120 130 140 150
DAFGDIQFET LGKKGKYLRL SCDTDSETLY ELLTQHWHLK TPNLVISVTG
160 170 180 190 200
GAKNFALKPR MRKIFSRLIY IAQSKGAWIL TGGTHYGLMK YIGEVVRDNT
210 220 230 240 250
ISRNSEENIV AIGIAAWGMV SNRDTLIRNC DDEGHFSAQY IMDDFMRDPL
260 270 280 290 300
YILDNNHTHL LLVDNGCHGH PTVEAKLRNQ LEKYISERTS QDSNYGGKIP
310 320 330 340 350
IVCFAQGGGR ETLKAINTSV KSKIPCVVVE GSGQIADVIA SLVEVEDVLT
360 370 380 390 400
SSMVKEKLVR FLPRTVSRLP EEEIESWIKW LKEILESPHL LTVIKMEEAG
410 420 430 440 450
DEVVSSAISY ALYKAFSTNE QDKDNWNGQL KLLLEWNQLD LASDEIFTND
460 470 480 490 500
RRWESADLQE VMFTALIKDR PKFVRLFLEN GLNLQKFLTN EVLTELFSTH
510 520 530 540 550
FSTLVYRNLQ IAKNSYNDAL LTFVWKLVAN FRRSFWKEDR SSREDLDVEL
560 570 580 590 600
HDASLTTRHP LQALFIWAIL QNKKELSKVI WEQTKGCTLA ALGASKLLKT
610 620 630 640 650
LAKVKNDINA AGESEELANE YETRAVELFT ECYSSDEDLA EQLLVYSCEA
660 670 680 690 700
WGGSNCLELA VEATDQHFIA QPGVQNFLSK QWYGEISRDT KNWKIILCLF
710 720 730 740 750
IIPLVGCGLV SFRKKPIDKH KKLLWYYVAF FTSPFVVFSW NVVFYIAFLL
760 770 780 790 800
LFAYVLLMDF HSVPHTPELI LYALVFVLFC DEVRQWYMNG VNYFTDLWNV
810 820 830 840 850
MDTLGLFYFI AGIVFRLHSS NKSSLYSGRV IFCLDYIIFT LRLIHIFTVS
860 870 880 890 900
RNLGPKIIML QRMLIDVFFF LFLFAVWMVA FGVARQGILR QNEQRWRWIF
910 920 930 940 950
RSVIYEPYLA MFGQVPSDVD STTYDFSHCT FSGNESKPLC VELDEYNLPR
960 970 980 990 1000
FPEWITIPLV CIYMLSTNIL LVNLLVAMFG YTVGIVQENN DQVWKFQRYF
1010 1020 1030 1040 1050
LVQEYCNRLN IPFPFVVFAY FYMVVKKCFK CCCKEKNTES SACCFRNEDN
1060 1070 1080 1090 1100
ETLAWEGVMK ENYLVKINTK ANDNAEEMRH RFRQLDTKLN DLKGLLKEIA

NKIK
Length:1,104
Mass (Da):127,629
Last modified:January 4, 2005 - v4
Checksum:iD07BFF14EB23970A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY072788 mRNA Translation: AAL68394.2
RefSeqiNP_599198.2, NM_134371.2
UniGeneiRn.81225

Genome annotation databases

EnsembliENSRNOT00000025879; ENSRNOP00000025879; ENSRNOG00000019035
GeneIDi171384
KEGGirno:171384

Similar proteinsi

Entry informationi

Entry nameiTRPM8_RAT
AccessioniPrimary (citable) accession number: Q8R455
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: January 4, 2005
Last modified: May 23, 2018
This is version 101 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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