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Protein

Monoglyceride lipase

Gene

Mgll

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain. Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth.By similarity1 Publication

Catalytic activityi

Hydrolyzes glycerol monoesters of long-chain fatty acids.By similarity

Pathwayi: triacylglycerol degradation

This protein is involved in the pathway triacylglycerol degradation, which is part of Glycerolipid metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway triacylglycerol degradation and in Glycerolipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei122 – 1221NucleophileBy similarity
Active sitei239 – 2391Charge relay systemBy similarity
Active sitei269 – 2691Charge relay systemBy similarity

GO - Molecular functioni

  • acylglycerol lipase activity Source: UniProtKB
  • hydrolase activity Source: RGD
  • lipid binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BRENDAi3.1.1.23. 5301.
ReactomeiR-RNO-1482883. Acyl chain remodeling of DAG and TAG.
R-RNO-163560. Hormone-sensitive lipase (HSL)-mediated triacylglycerol hydrolysis.
R-RNO-426048. Arachidonate production from DAG.
UniPathwayiUPA00256.

Protein family/group databases

ESTHERiratno-MGLL. Monoglyceridelipase_lysophospholip.
MEROPSiS33.979.

Chemistry

SwissLipidsiSLP:000001427.

Names & Taxonomyi

Protein namesi
Recommended name:
Monoglyceride lipaseCurated (EC:3.1.1.23By similarity)
Short name:
MGL
Alternative name(s):
Monoacylglycerol lipase
Short name:
MAGL
Gene namesi
Name:MgllImported
Synonyms:Mgl2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi71039. Mgll.

Subcellular locationi

  • Cytoplasmcytosol By similarity
  • Membrane By similarity; Peripheral membrane protein By similarity

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • extrinsic component of membrane Source: UniProtKB
  • lipid particle Source: Reactome
  • membrane Source: GO_Central
  • synapse Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3321.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 303303Monoglyceride lipasePRO_0000191267Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei10 – 101PhosphothreonineBy similarity
Modified residuei58 – 581Nitrated tyrosineBy similarity
Modified residuei189 – 1891PhosphoserineBy similarity

Keywords - PTMi

Nitration, Phosphoprotein

Proteomic databases

PaxDbiQ8R431.
PRIDEiQ8R431.

PTM databases

iPTMnetiQ8R431.
PhosphoSiteiQ8R431.
SwissPalmiQ8R431.

Expressioni

Tissue specificityi

Ubiquitous. Highly expressed in adipose tissue, adrenal gland, ovary, heart, spleen, lung, skeletal muscle, kidney and testis. Highly expressed throughout the brain.2 Publications

Gene expression databases

BgeeiENSRNOG00000014508.
ExpressionAtlasiQ8R431. baseline and differential.
GenevisibleiQ8R431. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

MINTiMINT-4580167.
STRINGi10116.ENSRNOP00000030271.

Chemistry

BindingDBiQ8R431.

Structurei

3D structure databases

ProteinModelPortaliQ8R431.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1455. Eukaryota.
COG2267. LUCA.
GeneTreeiENSGT00390000011364.
HOGENOMiHOG000003227.
HOVERGENiHBG049220.
InParanoidiQ8R431.
KOiK01054.
PhylomeDBiQ8R431.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR022742. Hydrolase_4.
[Graphical view]
PfamiPF12146. Hydrolase_4. 1 hit.
[Graphical view]
PRINTSiPR00111. ABHYDROLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R431-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEASSPRRT PQNVPYQDLP HLVNADGQYL FCRYWKPSGT PKALIFVSHG
60 70 80 90 100
AGEHCGRYDE LAQMLKRLDM LVFAHDHVGH GQSEGERMVV SDFQVFVRDL
110 120 130 140 150
LQHVNTVQKD YPEVPVFLLG HSMGGAISIL AAAERPTHFS GMILISPLIL
160 170 180 190 200
ANPESASTLK VLAAKLLNFV LPNISLGRID SSVLSRNKSE VDLYNSDPLI
210 220 230 240 250
CHAGVKVCFG IQLLNAVSRV ERAMPRLTLP FLLLQGSADR LCDSKGAYLL
260 270 280 290 300
MESSPSQDKT LKMYEGAYHV LHKELPEVTN SVLHEINTWV SHRIAVAGAR

CLP
Length:303
Mass (Da):33,500
Last modified:June 1, 2002 - v1
Checksum:i49CD407D97A1A971
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY081195 mRNA. Translation: AAL87453.1.
BC107920 mRNA. Translation: AAI07921.1.
RefSeqiNP_612511.1. NM_138502.2.
UniGeneiRn.40396.

Genome annotation databases

EnsembliENSRNOT00000029125; ENSRNOP00000030271; ENSRNOG00000014508.
GeneIDi29254.
KEGGirno:29254.
UCSCiRGD:71039. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY081195 mRNA. Translation: AAL87453.1.
BC107920 mRNA. Translation: AAI07921.1.
RefSeqiNP_612511.1. NM_138502.2.
UniGeneiRn.40396.

3D structure databases

ProteinModelPortaliQ8R431.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4580167.
STRINGi10116.ENSRNOP00000030271.

Chemistry

BindingDBiQ8R431.
ChEMBLiCHEMBL3321.
SwissLipidsiSLP:000001427.

Protein family/group databases

ESTHERiratno-MGLL. Monoglyceridelipase_lysophospholip.
MEROPSiS33.979.

PTM databases

iPTMnetiQ8R431.
PhosphoSiteiQ8R431.
SwissPalmiQ8R431.

Proteomic databases

PaxDbiQ8R431.
PRIDEiQ8R431.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000029125; ENSRNOP00000030271; ENSRNOG00000014508.
GeneIDi29254.
KEGGirno:29254.
UCSCiRGD:71039. rat.

Organism-specific databases

CTDi11343.
RGDi71039. Mgll.

Phylogenomic databases

eggNOGiKOG1455. Eukaryota.
COG2267. LUCA.
GeneTreeiENSGT00390000011364.
HOGENOMiHOG000003227.
HOVERGENiHBG049220.
InParanoidiQ8R431.
KOiK01054.
PhylomeDBiQ8R431.

Enzyme and pathway databases

UniPathwayiUPA00256.
BRENDAi3.1.1.23. 5301.
ReactomeiR-RNO-1482883. Acyl chain remodeling of DAG and TAG.
R-RNO-163560. Hormone-sensitive lipase (HSL)-mediated triacylglycerol hydrolysis.
R-RNO-426048. Arachidonate production from DAG.

Miscellaneous databases

PROiQ8R431.

Gene expression databases

BgeeiENSRNOG00000014508.
ExpressionAtlasiQ8R431. baseline and differential.
GenevisibleiQ8R431. RN.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR022742. Hydrolase_4.
[Graphical view]
PfamiPF12146. Hydrolase_4. 1 hit.
[Graphical view]
PRINTSiPR00111. ABHYDROLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMGLL_RAT
AccessioniPrimary (citable) accession number: Q8R431
Secondary accession number(s): Q32PZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: June 1, 2002
Last modified: September 7, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Requires non-ionic detergent for solubilization.
Short-term inhibition causes analgesia, while long-term inhibition causes tolerance to endocannabinoids acting on brain cannabinoid receptor CNR1, and a reduction in brain cannabinoid receptor CNR1 activity.By similarity

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.