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Reviewed, UniProtKB/Swiss-Prot Q8R429 (AT2A1_MOUSE)

Last modified November 3, 2009. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
      Short name=SERCA1
    EC=3.6.3.8
Alternative name(s):
    Calcium pump 1
    Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform
    SR Ca(2+)-ATPase 1
    Endoplasmic reticulum class 1/2 Ca(2+) ATPase
Gene names
Name: Atp2a1
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length994 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction By similarity.

Catalytic activity

ATP + H2O + Ca2+(Cis) = ADP + phosphate + Ca2+(Trans).

Enzyme regulation

Reversibly inhibited by phospholamban (PLN) at low calcium concentrations. Dephosphorylated PLN decreases the apparent affinity of the ATPase for calcium. This inhibition is regulated by the phosphorylation of PLN By similarity.

Subunit structure

Associated with sarcolipin (SLN) and phospholamban (PLN) By similarity.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the cation transport ATPase (P-type) family. Type IIA subfamily.

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Ontologies

Keywords
   Biological processCalcium transport
Ion transport
Transport
   Cellular componentEndoplasmic reticulum
Membrane
Sarcoplasmic reticulum
   DomainTransmembrane
   LigandATP-binding
Calcium
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionHydrolase
   PTMDisulfide bond
Phosphoprotein
Gene Ontology (GO)
   Biological processATP biosynthetic process

Inferred from electronic annotation. Source: InterPro

ATP catabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

calcium ion transport

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of striated muscle contraction

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of fast-twitch skeletal muscle fiber contraction

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular componentER-Golgi intermediate compartment

Inferred from sequence or structural similarity. Source: UniProtKB

H zone

Inferred from sequence or structural similarity. Source: UniProtKB

I band

Inferred from sequence or structural similarity. Source: UniProtKB

endoplasmic reticulum membrane

Inferred from sequence or structural similarity. Source: UniProtKB

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

perinuclear region of cytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

sarcoplasmic reticulum

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular functionATP binding

Inferred from sequence or structural similarity. Source: UniProtKB

calcium ion binding

Inferred from sequence or structural similarity. Source: UniProtKB

calcium-transporting ATPase activity

Inferred from sequence or structural similarity. Source: UniProtKB

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 994994Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
PRO_0000046188

Regions

Topological domain1 – 4848Cytoplasmic By similarity
Transmembrane49 – 69211 By similarity
Topological domain70 – 8920Lumenal By similarity
Transmembrane90 – 110212 By similarity
Topological domain111 – 253143Cytoplasmic By similarity
Transmembrane254 – 273203 By similarity
Topological domain274 – 29522Lumenal By similarity
Transmembrane296 – 313184 By similarity
Topological domain314 – 757444Cytoplasmic By similarity
Transmembrane758 – 777205 By similarity
Topological domain778 – 78710Lumenal By similarity
Transmembrane788 – 808216 By similarity
Topological domain809 – 82820Cytoplasmic By similarity
Transmembrane829 – 851237 By similarity
Topological domain852 – 89746Lumenal By similarity
Transmembrane898 – 917208 By similarity
Topological domain918 – 93013Cytoplasmic By similarity
Transmembrane931 – 949199 By similarity
Topological domain950 – 96415Lumenal By similarity
Transmembrane965 – 9852110 By similarity
Topological domain986 – 9949Cytoplasmic By similarity
Region370 – 40031Interacts with phospholamban 1 By similarity
Region788 – 80821Interacts with phospholamban 2 By similarity

Sites

Active site35114-aspartylphosphate intermediate By similarity
Metal binding3041Calcium 2; via carbonyl oxygen By similarity
Metal binding3051Calcium 2; via carbonyl oxygen By similarity
Metal binding3071Calcium 2; via carbonyl oxygen By similarity
Metal binding3091Calcium 2 By similarity
Metal binding7031Magnesium By similarity
Metal binding7071Magnesium By similarity
Metal binding7681Calcium 1 By similarity
Metal binding7711Calcium 1 By similarity
Metal binding7961Calcium 2 By similarity
Metal binding7991Calcium 1 By similarity
Metal binding8001Calcium 1 By similarity
Metal binding8001Calcium 2 By similarity
Metal binding9081Calcium 1 By similarity

Amino acid modifications

Disulfide bond876 ↔ 888 By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q8R429-1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 884FBDD286B4916B

FASTA994109,425
        10         20         30         40         50         60 
MEAAHSKSTE ECLSYFGVSE TTGLTPDQVK RHLEKYGPNE LPAEEGKSLW ELVVEQFEDL 

        70         80         90        100        110        120 
LVRILLLAAC ISFVLAWFEE GEETVTAFVE PFVILLILIA NAIVGVWQER NAENAIEALK 

       130        140        150        160        170        180 
EYEPEMGKVY RADRKSVQRI KARDIVPGDI VEVAVGDKVP ADIRILSIKS TTLRVDQSIL 

       190        200        210        220        230        240 
TGESVSVIKH TDPVPDPRAV NQDKKNMLFS GTNIAAGKAV GIVATTGVST EIGKIRDQMA 

       250        260        270        280        290        300 
ATEQDKTPLQ QKLDEFGEQL SKVISLICVA VWLINIGHFN DPVHGGSWFR GAIYYFKIAV 

       310        320        330        340        350        360 
ALAVAAIPEG LPAVITTCLA LGTRRMAKKN AIVRSLPSVE TLGCTSVICS DKTGTLTTNQ 

       370        380        390        400        410        420 
MSVCKMFIID KVDGDVCSLN EFSITGSTYA PEGEVLKNDK PVRAGQYDGL VELATICALC 

       430        440        450        460        470        480 
NDSSLDFNET KGVYEKVGEA TETALTTLVE KMNVFNTEVR SLSKVERANA CNSVIRQLMK 

       490        500        510        520        530        540 
KEFTLEFSRD RKSMSVYCSP AKSSRAAVGN KMFVKGAPEG VIDRCNYVRV GTTRVPLTGP 

       550        560        570        580        590        600 
VKEKIMSVIK EWGTGRDTLR CLALATRDTP PKREEMVLDD SAKFMEYEMD LTFVGVVGML 

       610        620        630        640        650        660 
DPPRKEVTGS IQLCRDAGIR VIMITGDNKG TAIAICRRIG IFSENEEVTD RAYTGREFDD 

       670        680        690        700        710        720 
LPLAEQREAC RRACCFARVE PSHKSKIVEY LQSYDEITAM TGDGVNDAPA LKKAEIGIAM 

       730        740        750        760        770        780 
GSGTAVAKTA SEMVLADDNF STIVAAVEEG RAIYNNMKQF IRYLISSNVG EVVCIFLTAA 

       790        800        810        820        830        840 
LGLPEALIPV QLLWVNLVTD GLPATALGFN PPDLDIMDRP PRSPKEPLIS GWLFFRYMAI 

       850        860        870        880        890        900 
GGYVGAATVG AAAWWFLYAE DGPHVSYHQL THFMQCTEHN PEFDGLDCEV FEAPEPMTMA 

       910        920        930        940        950        960 
LSVLVTIEMC NALNSLSENQ SLLRMPPWVN IWLLGSICLS MSLHFLILYV DPLPMIFKLR 

       970        980        990 
ALDFTQWLMV LKISLPVIGL DELLKFIARN YLEG

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References

« Hide 'large scale' references
[1]"Towards a complete inventory of calcium transporters of the house mouse."
Kraev A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c X CD-1.
Tissue: Skeletal muscle.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Salivary gland.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AY081946 mRNA. Translation: AAL87408.1.
BC036292 mRNA. Translation: AAH36292.1.
IPIIPI00311654.
RefSeqNP_031530.2.
UniGeneMm.35134

3D structure databases

HSSPHSSP built from PDB template 1SU4 based on UniProtKB P04191.
SMRQ8R429. Positions 1-994.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ8R429.

PTM databases

PhosphoSiteQ8R429.

Proteomic databases

PRIDEQ8R429.

Genome annotation databases

EnsemblENSMUST00000032974; ENSMUSP00000032974; ENSMUSG00000030730; Mus musculus. [Genome view]
GeneID11937.
KEGGmmu:11937.
NMPDRfig|10090.3.peg.17619.
UCSCuc009jrf.1. mouse.

Organism-specific databases

CTD11937.
MGIMGI:105058. Atp2a1.

Phylogenomic databases

HOGENOMQ8R429.
HOVERGENQ8R429.
OMAQVKRNLE.

Enzyme and pathway databases

BRENDA3.6.3.8. 244.

Gene expression databases

ArrayExpressQ8R429.
BgeeQ8R429.
GenevestigatorQ8R429.
GermOnlineENSMUSG00000030730. Mus musculus.

Family and domain databases

InterProIPR008250. ATPase_P-typ_ATPase-assoc-reg.
IPR005782. ATPase_P-typ_Ca-transp.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR000695. ATPase_P-typ_H-transp.
IPR001757. ATPase_P-typ_ion-transptr.
IPR018303. ATPase_P-typ_P_site.
IPR005834. Dehalogen-like_hydro.
[Graphical view]
PANTHERPTHR11939. ATPase_P. 1 hit.
PfamPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
PR00120. HATPASE.
TIGRFAMsTIGR01116. ATPase-IIA1_Ca. 1 hit.
TIGR01494. ATPase_P-type. 4 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio280035.
SOURCESearch...

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Entry information

Entry nameAT2A1_MOUSE
AccessionPrimary (citable) accession number: Q8R429
Entry history
Integrated into UniProtKB/Swiss-Prot: May 24, 2005
Last sequence update: June 1, 2002
Last modified: November 3, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents