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Protein

Kv channel-interacting protein 1

Gene

Kcnip1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels (PubMed:14980206). Regulates channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner (PubMed:14980206). Modulates KCND2/Kv4.2 currents (PubMed:14980206). In vitro, modulates KCND1/Kv4.1 currents (By similarity). Increases the presence of KCND2 at the cell surface (PubMed:14980206).By similarity2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi146 – 157121PROSITE-ProRule annotation1 PublicationAdd
BLAST
Calcium bindingi194 – 205122PROSITE-ProRule annotation1 PublicationAdd
BLAST

GO - Molecular functioni

  • calcium ion binding Source: RGD
  • ion channel binding Source: RGD
  • potassium channel activity Source: UniProtKB-KW
  • potassium channel regulator activity Source: RGD
  • protein heterodimerization activity Source: RGD
  • protein N-terminus binding Source: RGD
  • voltage-gated ion channel activity Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Calcium, Metal-binding, Potassium

Enzyme and pathway databases

ReactomeiR-RNO-5576894. Phase 1 - inactivation of fast Na+ channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Kv channel-interacting protein 1
Short name:
KChIP1
Alternative name(s):
A-type potassium channel modulatory protein 1
Potassium channel-interacting protein 1
Gene namesi
Name:Kcnip1
Synonyms:Kchip1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi70886. Kcnip1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • dendrite Source: RGD
  • extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
  • neuronal cell body Source: RGD
  • plasma membrane Source: RGD
  • potassium channel complex Source: RGD
  • voltage-gated potassium channel complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi214 – 22714Missing : Abolishes interaction with KCND2. 1 PublicationAdd
BLAST

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 227227Kv channel-interacting protein 1PRO_0000073820Add
BLAST

Proteomic databases

PaxDbiQ8R426.

Expressioni

Tissue specificityi

Detected in hippocampus and in the molecular layer of the dentate gyrus (at protein level) (PubMed:15356203). Isoform 1 and isoform 2 are predominantly expressed at equal levels in brain. Colocalizes with KCND3 in inhibitory interneurons in cortex and hippocampus and in striatal interneurons.3 Publications

Gene expression databases

GenevisibleiQ8R426. RN.

Interactioni

Subunit structurei

Component of heteromultimeric potassium channels (PubMed:15356203, PubMed:14980206). Identified in potassium channel complexes containing KCND1, KCND2, KCND3, KCNIP1, KCNIP2, KCNIP3, KCNIP4, DPP6 and DPP10 (By similarity). Part of a heterooctamer composed of the tetrameric channel and four KCNIP1 chains (By similarity). Interacts with KCND3 and the N-terminal domain of KCND2. Probably part of a complex consisting of KCNIP1, KCNIP2 isoform 3 and KCND2 (PubMed:14980206). Self-associates to form homodimers and homotetramers. Interacts with KCNIP2 isoform 3 in a calcium-dependent manner.By similarity4 Publications

GO - Molecular functioni

  • ion channel binding Source: RGD
  • protein heterodimerization activity Source: RGD
  • protein N-terminus binding Source: RGD

Protein-protein interaction databases

BioGridi249216. 1 interaction.
IntActiQ8R426. 1 interaction.
STRINGi10116.ENSRNOP00000007187.

Structurei

Secondary structure

1
227
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi51 – 566Combined sources
Helixi61 – 7414Combined sources
Beta strandi78 – 803Combined sources
Helixi82 – 9211Combined sources
Helixi99 – 10911Combined sources
Helixi119 – 13113Combined sources
Helixi134 – 14512Combined sources
Helixi155 – 16814Combined sources
Helixi183 – 19311Combined sources
Beta strandi198 – 2014Combined sources
Helixi203 – 2097Combined sources
Helixi215 – 22713Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1S6CX-ray2.00A45-227[»]
ProteinModelPortaliQ8R426.
SMRiQ8R426. Positions 49-227.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8R426.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 9457EF-hand 1; degeneratePROSITE-ProRule annotationAdd
BLAST
Domaini97 – 13236EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini133 – 16836EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini181 – 21636EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni214 – 22714Interaction with KCND21 PublicationAdd
BLAST

Sequence similaritiesi

Belongs to the recoverin family.Curated
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiQ8R426.
OMAiTDAPKQH.
OrthoDBiEOG7GJ6F3.
PhylomeDBiQ8R426.
TreeFamiTF318560.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PfamiPF13499. EF-hand_7. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R426-1) [UniParc]FASTAAdd to basket

Also known as: KChIP1b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGAVMGTFSS LQTKQRRPSK DIAWWYYQYQ RDKIEDDLEM TMVCHRPEGL
60 70 80 90 100
EQLEAQTNFT KRELQVLYRG FKNECPSGVV NEETFKQIYA QFFPHGDAST
110 120 130 140 150
YAHYLFNAFD TTQTGSVKFE DFVTALSILL RGTVHEKLRW TFNLYDINKD
160 170 180 190 200
GYINKEEMMD IVKAIYDMMG KYTYPVLKED TPRQHVDVFF QKMDKNKDGI
210 220
VTLDEFLESC QEDDNIMRSL QLFQNVM
Length:227
Mass (Da):26,817
Last modified:August 16, 2005 - v2
Checksum:i92A2F0D72A120197
GO
Isoform 2 (identifier: Q8R426-2) [UniParc]FASTAAdd to basket

Also known as: KChIP1a

The sequence of this isoform differs from the canonical sequence as follows:
     21-31: Missing.

Show »
Length:216
Mass (Da):25,244
Checksum:iA9076FED82B707F6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 101S → P in AAN34942 (PubMed:12646414).Curated
Sequence conflicti37 – 371D → E in BAB03308 (PubMed:11263977).Curated
Sequence conflicti107 – 1071N → D in AAL92564 (Ref. 2) Curated
Sequence conflicti107 – 1071N → D in AAL92565 (Ref. 2) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei21 – 3111Missing in isoform 2. 2 PublicationsVSP_015048Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046443 mRNA. Translation: BAB03308.1.
AY082657 mRNA. Translation: AAL92564.1.
AY082658 mRNA. Translation: AAL92565.1.
AY142709 mRNA. Translation: AAN34942.1.
RefSeqiNP_001248318.1. NM_001261389.1. [Q8R426-1]
NP_075218.2. NM_022929.2. [Q8R426-2]
UniGeneiRn.20530.

Genome annotation databases

GeneIDi65023.
KEGGirno:65023.
UCSCiRGD:70886. rat. [Q8R426-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046443 mRNA. Translation: BAB03308.1.
AY082657 mRNA. Translation: AAL92564.1.
AY082658 mRNA. Translation: AAL92565.1.
AY142709 mRNA. Translation: AAN34942.1.
RefSeqiNP_001248318.1. NM_001261389.1. [Q8R426-1]
NP_075218.2. NM_022929.2. [Q8R426-2]
UniGeneiRn.20530.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1S6CX-ray2.00A45-227[»]
ProteinModelPortaliQ8R426.
SMRiQ8R426. Positions 49-227.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249216. 1 interaction.
IntActiQ8R426. 1 interaction.
STRINGi10116.ENSRNOP00000007187.

Proteomic databases

PaxDbiQ8R426.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi65023.
KEGGirno:65023.
UCSCiRGD:70886. rat. [Q8R426-1]

Organism-specific databases

CTDi30820.
RGDi70886. Kcnip1.

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiQ8R426.
OMAiTDAPKQH.
OrthoDBiEOG7GJ6F3.
PhylomeDBiQ8R426.
TreeFamiTF318560.

Enzyme and pathway databases

ReactomeiR-RNO-5576894. Phase 1 - inactivation of fast Na+ channels.

Miscellaneous databases

EvolutionaryTraceiQ8R426.
NextBioi613784.
PROiQ8R426.

Gene expression databases

GenevisibleiQ8R426. RN.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PfamiPF13499. EF-hand_7. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and expression of the novel splice variants of K(+) channel-interacting protein 2."
    Ohya S., Morohashi Y., Muraki K., Tomita T., Watanabe M., Iwatsubo T., Imaizumi Y.
    Biochem. Biophys. Res. Commun. 282:96-102(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Brain.
  2. Takimoto K.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Strain: Sprague-Dawley.
  3. "Functional properties of a brain-specific NH2-terminally spliced modulator of Kv4 channels."
    Boland L.M., Jiang M., Lee S.Y., Fahrenkrug S.C., Harnett M.T., O'Grady S.M.
    Am. J. Physiol. 285:C161-C170(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
    Tissue: Brain.
  4. Cited for: INTERACTION WITH KCND2 AND KCND3, TISSUE SPECIFICITY.
  5. "Different effects of the Ca(2+)-binding protein, KChIP1, on two Kv4 subfamily members, Kv4.1 and Kv4.2."
    Nakamura T.Y., Nandi S., Pountney D.J., Artman M., Rudy B., Coetzee W.A.
    FEBS Lett. 499:205-209(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KCND2.
  6. "KChIPs and Kv4 alpha subunits as integral components of A-type potassium channels in mammalian brain."
    Rhodes K.J., Carroll K.I., Sung M.A., Doliveira L.C., Monaghan M.M., Burke S.L., Strassle B.W., Buchwalder L., Menegola M., Cao J., An W.F., Trimmer J.S.
    J. Neurosci. 24:7903-7915(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INTERACTION WITH KCND2, SUBCELLULAR LOCATION.
  7. "Structural insights into the functional interaction of KChIP1 with Shal-type K(+) channels."
    Zhou W., Qian Y., Kunjilwar K., Pfaffinger P.J., Choe S.
    Neuron 41:573-586(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 45-216 IN COMPLEX WITH CALCIUM IONS AND KCND2, INTERACTION WITH KCND2, CALCIUM-BINDING, FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 214-ASP--MET-227.

Entry informationi

Entry nameiKCIP1_RAT
AccessioniPrimary (citable) accession number: Q8R426
Secondary accession number(s): Q793P9, Q8CIR1, Q8R425
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: August 16, 2005
Last modified: May 11, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.