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Protein

Kv channel-interacting protein 1

Gene

Kcnip1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels (PubMed:14980206). Regulates channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner (PubMed:14980206). Modulates KCND2/Kv4.2 currents (PubMed:14980206). In vitro, modulates KCND1/Kv4.1 currents (By similarity). Increases the presence of KCND2 at the cell surface (PubMed:14980206).By similarity2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi146 – 1571PROSITE-ProRule annotation1 PublicationAdd BLAST12
Calcium bindingi194 – 2052PROSITE-ProRule annotation1 PublicationAdd BLAST12

GO - Molecular functioni

  • calcium ion binding Source: RGD
  • ion channel binding Source: RGD
  • potassium channel activity Source: UniProtKB-KW
  • potassium channel regulator activity Source: RGD
  • protein heterodimerization activity Source: RGD
  • protein N-terminus binding Source: RGD
  • voltage-gated ion channel activity Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Calcium, Metal-binding, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Kv channel-interacting protein 1
Short name:
KChIP1
Alternative name(s):
A-type potassium channel modulatory protein 1
Potassium channel-interacting protein 1
Gene namesi
Name:Kcnip1
Synonyms:Kchip1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi70886. Kcnip1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • dendrite Source: RGD
  • extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
  • neuronal cell body Source: RGD
  • plasma membrane Source: RGD
  • potassium channel complex Source: RGD
  • voltage-gated potassium channel complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi214 – 227Missing : Abolishes interaction with KCND2. 1 PublicationAdd BLAST14

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000738201 – 227Kv channel-interacting protein 1Add BLAST227

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki61Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ8R426.
PRIDEiQ8R426.

Expressioni

Tissue specificityi

Detected in hippocampus and in the molecular layer of the dentate gyrus (at protein level) (PubMed:15356203). Isoform 1 and isoform 2 are predominantly expressed at equal levels in brain. Colocalizes with KCND3 in inhibitory interneurons in cortex and hippocampus and in striatal interneurons.3 Publications

Gene expression databases

BgeeiENSRNOG00000005365.
GenevisibleiQ8R426. RN.

Interactioni

Subunit structurei

Component of heteromultimeric potassium channels (PubMed:15356203, PubMed:14980206). Identified in potassium channel complexes containing KCND1, KCND2, KCND3, KCNIP1, KCNIP2, KCNIP3, KCNIP4, DPP6 and DPP10 (By similarity). Part of a heterooctamer composed of the tetrameric channel and four KCNIP1 chains (By similarity). Interacts with KCND3 and the N-terminal domain of KCND2. Probably part of a complex consisting of KCNIP1, KCNIP2 isoform 3 and KCND2 (PubMed:14980206). Self-associates to form homodimers and homotetramers. Interacts with KCNIP2 isoform 3 in a calcium-dependent manner.By similarity4 Publications

GO - Molecular functioni

  • ion channel binding Source: RGD
  • protein heterodimerization activity Source: RGD
  • protein N-terminus binding Source: RGD

Protein-protein interaction databases

BioGridi249216. 1 interactor.
IntActiQ8R426. 1 interactor.
STRINGi10116.ENSRNOP00000007187.

Structurei

Secondary structure

1227
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi51 – 56Combined sources6
Helixi61 – 74Combined sources14
Beta strandi78 – 80Combined sources3
Helixi82 – 92Combined sources11
Helixi99 – 109Combined sources11
Helixi119 – 131Combined sources13
Helixi134 – 145Combined sources12
Helixi155 – 168Combined sources14
Helixi183 – 193Combined sources11
Beta strandi198 – 201Combined sources4
Helixi203 – 209Combined sources7
Helixi215 – 227Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S6CX-ray2.00A45-227[»]
ProteinModelPortaliQ8R426.
SMRiQ8R426.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8R426.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 94EF-hand 1; degeneratePROSITE-ProRule annotationAdd BLAST57
Domaini97 – 132EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini133 – 168EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini181 – 216EF-hand 4PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni214 – 227Interaction with KCND21 PublicationAdd BLAST14

Sequence similaritiesi

Belongs to the recoverin family.Curated
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiQ8R426.
OrthoDBiEOG091G11T4.
PhylomeDBiQ8R426.
TreeFamiTF318560.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PfamiPF13499. EF-hand_7. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R426-1) [UniParc]FASTAAdd to basket
Also known as: KChIP1b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGAVMGTFSS LQTKQRRPSK DIAWWYYQYQ RDKIEDDLEM TMVCHRPEGL
60 70 80 90 100
EQLEAQTNFT KRELQVLYRG FKNECPSGVV NEETFKQIYA QFFPHGDAST
110 120 130 140 150
YAHYLFNAFD TTQTGSVKFE DFVTALSILL RGTVHEKLRW TFNLYDINKD
160 170 180 190 200
GYINKEEMMD IVKAIYDMMG KYTYPVLKED TPRQHVDVFF QKMDKNKDGI
210 220
VTLDEFLESC QEDDNIMRSL QLFQNVM
Length:227
Mass (Da):26,817
Last modified:August 16, 2005 - v2
Checksum:i92A2F0D72A120197
GO
Isoform 2 (identifier: Q8R426-2) [UniParc]FASTAAdd to basket
Also known as: KChIP1a

The sequence of this isoform differs from the canonical sequence as follows:
     21-31: Missing.

Show »
Length:216
Mass (Da):25,244
Checksum:iA9076FED82B707F6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10S → P in AAN34942 (PubMed:12646414).Curated1
Sequence conflicti37D → E in BAB03308 (PubMed:11263977).Curated1
Sequence conflicti107N → D in AAL92564 (Ref. 2) Curated1
Sequence conflicti107N → D in AAL92565 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01504821 – 31Missing in isoform 2. 2 PublicationsAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046443 mRNA. Translation: BAB03308.1.
AY082657 mRNA. Translation: AAL92564.1.
AY082658 mRNA. Translation: AAL92565.1.
AY142709 mRNA. Translation: AAN34942.1.
RefSeqiNP_001248318.1. NM_001261389.1. [Q8R426-1]
NP_075218.2. NM_022929.2. [Q8R426-2]
UniGeneiRn.20530.

Genome annotation databases

GeneIDi65023.
KEGGirno:65023.
UCSCiRGD:70886. rat. [Q8R426-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046443 mRNA. Translation: BAB03308.1.
AY082657 mRNA. Translation: AAL92564.1.
AY082658 mRNA. Translation: AAL92565.1.
AY142709 mRNA. Translation: AAN34942.1.
RefSeqiNP_001248318.1. NM_001261389.1. [Q8R426-1]
NP_075218.2. NM_022929.2. [Q8R426-2]
UniGeneiRn.20530.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S6CX-ray2.00A45-227[»]
ProteinModelPortaliQ8R426.
SMRiQ8R426.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249216. 1 interactor.
IntActiQ8R426. 1 interactor.
STRINGi10116.ENSRNOP00000007187.

Proteomic databases

PaxDbiQ8R426.
PRIDEiQ8R426.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi65023.
KEGGirno:65023.
UCSCiRGD:70886. rat. [Q8R426-1]

Organism-specific databases

CTDi30820.
RGDi70886. Kcnip1.

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiQ8R426.
OrthoDBiEOG091G11T4.
PhylomeDBiQ8R426.
TreeFamiTF318560.

Miscellaneous databases

EvolutionaryTraceiQ8R426.
PROiQ8R426.

Gene expression databases

BgeeiENSRNOG00000005365.
GenevisibleiQ8R426. RN.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PfamiPF13499. EF-hand_7. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKCIP1_RAT
AccessioniPrimary (citable) accession number: Q8R426
Secondary accession number(s): Q793P9, Q8CIR1, Q8R425
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: August 16, 2005
Last modified: November 30, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.