Reviewed, UniProtKB/Swiss-Prot Q8R424 (STABP_RAT)

Names and origin

Protein names

Recommended name:
    STAM-binding protein
    EC=3.1.2.15
Alternative name(s):
    Associated molecule with the SH3 domain of STAM

Gene names

Name:	Stambp
Synonyms:	Amsh

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains By similarity. Functions at the endosome and is able to oppose the ubiquitin-dependent sorting of receptors to lysosomes. Endosomal localization of AMSH is required for efficient EGFR degradation but not for its internalization. Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7 By similarity.
Catalytic activity	Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol.
Cofactor	Binds 2 zinc ion per subunit By similarity.
Enzyme regulation	Inhibited by N-ethylmaleimide By similarity.
Subunit structure	Interacts with STAM1. Interacts with SMAD6 and SMAD7. Interacts with CHMP3; the interaction appears to relieve the autoinhibition of CHMP3 By similarity.
Subcellular location	Nucleus By similarity. Membrane; Peripheral membrane protein By similarity. Cytoplasm By similarity. Endosome By similarity.
Domain	The JAMM motif is essential for the protease activity By similarity.
Post-translational modification	Phosphorylated after BMP type I receptor activation By similarity.
Sequence similarities	Belongs to the peptidase M67C family. Contains 1 MPN (JAB/Mov34) domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm Endosome Membrane Nucleus
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endosome Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 424	424	STAM-binding protein		PRO_0000194871
Regions
	Domain	252 – 361	110	MPN
	Region	1 – 127	127	Interaction with CHMP3 By similarity
	Region	227 – 231	5	Interaction with STAM1 By similarity
	Motif	335 – 348	14	JAMM motif
	Compositional bias	73 – 127	55	Lys-rich
	Compositional bias	139 – 172	34	Gln-rich
Sites
	Metal binding	335	1	Zinc 1; catalytic By similarity
	Metal binding	337	1	Zinc 1; catalytic By similarity
	Metal binding	348	1	Zinc 1; catalytic By similarity
	Metal binding	350	1	Zinc 2 By similarity
	Metal binding	390	1	Zinc 2 By similarity
	Metal binding	396	1	Zinc 2 By similarity
	Metal binding	398	1	Zinc 2 By similarity
	Site	280	1	Indirect zinc-binding By similarity
Amino acid modifications
	Modified residue	2	1	Phosphoserine By similarity
	Modified residue	48	1	Phosphoserine By similarity
	Modified residue	243	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8R424-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 90BE083ADACF4B44	FASTA	424	48,512
	10 20 30 40 50 60 MSDHADVSLP PQDRVRILSQ LGSAVELNED IPPRRYFRSG VEIIRMASIY SEEGNIEHAF 70 80 90 100 110 120 ILYNKYITLF IEKLPKHRDY KSAIIPEKKD AVKKLKNVAF PKAEELKTEL LKRYTKEYEQ 130 140 150 160 170 180 YKERKKKEEE ELARNIAIQQ ELEKEKQRVA QQKQKQLEQE QFHAFEKMIQ KQELEKERLK 190 200 210 220 230 240 IVQEFGKVDP GPCGPLLPDL EKPCVDVAPS SPFSPTQTSD CNTTLRPAKP PVVDRSLKPG 250 260 270 280 290 300 ALSVIENVPT IEGLRHIVVP RNLCSEFLQL ASANTAKGIE TCGVLCGKLM RNEFTITHVL 310 320 330 340 350 360 IPRQNGGPDY CHTENEEEIF FMQDDLGLLT LGWIHTHPTQ TAFLSSVDLH THCSYQMMLP 370 380 390 400 410 420 ESIAIVCSPK FQETGFFKLT DYGLQEISTC RQKGFHPHGR DPPLFCDCSH VTVKDRIVTI TDLR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Pawlak A., Guellaen G. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY083159 mRNA. Translation: AAL92520.1. BC061711 mRNA. Translation: AAH61711.1.
IPI	IPI00200044.
RefSeq	NP_612540.1.
UniGene	Rn.14662
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M67.006.
PTM databases
PhosphoSite	Q8R424.
Genome annotation databases
Ensembl	ENSRNOG00000012227. Rattus norvegicus. [Contig view]
GeneID	171565.
KEGG	rno:171565.
Organism-specific databases
RGD	619963. Stambp.
Phylogenomic databases
HOVERGEN	Q8R424.
OMA	Q8R424. AVEINED.
Enzyme and pathway databases
BRENDA	3.1.2.15. 248.
Gene expression databases
ArrayExpress	Q8R424.
GermOnline	ENSRNOG00000012227. Rattus norvegicus.
Family and domain databases
InterPro	IPR000555. Mov34_MPN_PAD1. [Graphical view]
Pfam	PF01398. Mov34. 1 hit. [Graphical view]
SMART	SM00232. JAB_MPN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	622575.

Entry information

Entry name

STABP_RAT

Accession

Primary (citable) accession number: Q8R424

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)