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Q8R418

- DICER_MOUSE

UniProt

Q8R418 - DICER_MOUSE

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Protein

Endoribonuclease Dicer

Gene

Dicer1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes By similarity.By similarity
Isoform 2: More active than isoform 1 to process long double-stranded RNA into siRNAs. Responsible for the accumulation of endogenous siRNAs observed in mouse oocytes compared to somatic cells and it regulates meiotic spindle organization in female germline.

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Binds 2 magnesium or manganese ions per subunit.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi1316 – 13161Magnesium or manganese 1By similarity
Metal bindingi1395 – 13951Magnesium or manganese 1By similarity
Metal bindingi1398 – 13981Magnesium or manganese 1By similarity
Metal bindingi1699 – 16991Magnesium or manganese 2
Sitei1800 – 18001Important for activity
Metal bindingi1804 – 18041Magnesium or manganese 2
Metal bindingi1807 – 18071Magnesium or manganese 2

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi64 – 718ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. helicase activity Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
  4. miRNA binding Source: MGI
  5. ribonuclease III activity Source: UniProtKB

GO - Biological processi

  1. anatomical structure development Source: MGI
  2. angiogenesis Source: MGI
  3. branching morphogenesis of an epithelial tube Source: MGI
  4. cardiac muscle cell development Source: MGI
  5. cardiac neural crest cell development involved in outflow tract morphogenesis Source: MGI
  6. cartilage development Source: MGI
  7. cell proliferation Source: MGI
  8. cerebral cortex development Source: MGI
  9. defense response to virus Source: MGI
  10. digestive tract development Source: MGI
  11. embryonic hindlimb morphogenesis Source: MGI
  12. embryonic limb morphogenesis Source: MGI
  13. epidermis morphogenesis Source: MGI
  14. ganglion development Source: MGI
  15. hair follicle cell proliferation Source: MGI
  16. hair follicle development Source: MGI
  17. hair follicle morphogenesis Source: MGI
  18. inner ear receptor cell development Source: MGI
  19. intestinal epithelial cell development Source: MGI
  20. lung development Source: MGI
  21. meiotic spindle organization Source: UniProtKB
  22. mRNA stabilization Source: MGI
  23. multicellular organism growth Source: MGI
  24. myoblast differentiation involved in skeletal muscle regeneration Source: MGI
  25. negative regulation of Schwann cell proliferation Source: BHF-UCL
  26. negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  27. nerve development Source: BHF-UCL
  28. neuron projection morphogenesis Source: BHF-UCL
  29. olfactory bulb interneuron differentiation Source: MGI
  30. peripheral nervous system myelin formation Source: BHF-UCL
  31. positive regulation of gene expression Source: MGI
  32. positive regulation of miRNA metabolic process Source: MGI
  33. positive regulation of myelination Source: BHF-UCL
  34. positive regulation of Schwann cell differentiation Source: BHF-UCL
  35. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  36. post-embryonic development Source: MGI
  37. pre-miRNA processing Source: UniProtKB
  38. production of miRNAs involved in gene silencing by miRNA Source: BHF-UCL
  39. production of siRNA involved in RNA interference Source: UniProtKB
  40. regulation of cell cycle Source: MGI
  41. regulation of cell differentiation Source: MGI
  42. regulation of enamel mineralization Source: MGI
  43. regulation of gene expression Source: MGI
  44. regulation of miRNA metabolic process Source: MGI
  45. regulation of muscle cell apoptotic process Source: MGI
  46. regulation of myelination Source: MGI
  47. regulation of neurogenesis Source: MGI
  48. regulation of neuron differentiation Source: MGI
  49. regulation of Notch signaling pathway Source: BHF-UCL
  50. regulation of odontogenesis of dentin-containing tooth Source: MGI
  51. regulation of oligodendrocyte differentiation Source: MGI
  52. regulation of protein phosphorylation Source: BHF-UCL
  53. regulation of RNA metabolic process Source: MGI
  54. regulation of viral genome replication Source: MGI
  55. reproductive structure development Source: MGI
  56. RNA phosphodiester bond hydrolysis Source: GOC
  57. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
  58. RNA processing Source: MGI
  59. rRNA catabolic process Source: InterPro
  60. spinal cord motor neuron differentiation Source: MGI
  61. spindle assembly Source: MGI
  62. spleen development Source: MGI
  63. stem cell maintenance Source: MGI
  64. targeting of mRNA for destruction involved in RNA interference Source: UniProtKB
  65. zygote asymmetric cell division Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease

Keywords - Biological processi

RNA-mediated gene silencing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endoribonuclease Dicer (EC:3.1.26.3)
Alternative name(s):
Double-strand-specific ribonuclease mDCR-1
Gene namesi
Name:Dicer1
Synonyms:Dicer, Mdcr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:2177178. Dicer1.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. micro-ribonucleoprotein complex Source: MGI
  3. RISC complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Isoform 2: Mice lacking isoform 2 are viable and males are fertile. However, females are sterile, their oocytes displaying meiotic spindle defects.

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1800 – 18001K → A, R, S or T: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19161916Endoribonuclease DicerPRO_0000180471Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei413 – 4131PhosphoserineBy similarity
Modified residuei415 – 4151PhosphoserineBy similarity
Modified residuei1016 – 10161PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8R418.
PaxDbiQ8R418.
PRIDEiQ8R418.

PTM databases

PhosphoSiteiQ8R418.

Expressioni

Tissue specificityi

Isoform 1 is expressed in a wide variety of tissues. Isoform 2 is specifically expressed in oocytes during their growth (at protein level).

Gene expression databases

CleanExiMM_DICER1.
GenevestigatoriQ8R418.

Interactioni

Subunit structurei

Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2; DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. Note that the trimeric RLC/miRLC is also referred to as RISC. Interacts with DHX9, AGO1, PIWIL1 and PRKRA. Associates with the 60S ribosome. Interacts with BCDIN3D By similarity.By similarity

Protein-protein interaction databases

DIPiDIP-29721N.
IntActiQ8R418. 2 interactions.

Structurei

Secondary structure

1
1916
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi1649 – 16568
Turni1657 – 16593
Helixi1660 – 16678
Helixi1674 – 16818
Helixi1696 – 171621
Helixi1723 – 173311
Helixi1736 – 174510
Helixi1748 – 17503
Helixi1757 – 177418
Helixi1800 – 181617
Helixi1821 – 184222
Helixi1847 – 18548
Turni1856 – 18583
Beta strandi1859 – 18613
Beta strandi1872 – 18787
Turni1879 – 18813
Beta strandi1882 – 18909
Helixi1891 – 190818

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3C4BX-ray1.68A1648-1910[»]
3C4TX-ray2.80A1648-1910[»]
ProteinModelPortaliQ8R418.
SMRiQ8R418. Positions 766-1063, 1648-1910.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8R418.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini51 – 227177Helicase ATP-bindingAdd
BLAST
Domaini433 – 602170Helicase C-terminalAdd
BLAST
Domaini630 – 72293Dicer dsRNA-binding foldAdd
BLAST
Domaini891 – 1042152PAZAdd
BLAST
Domaini1276 – 1403128RNase III 1Add
BLAST
Domaini1660 – 1818159RNase III 2Add
BLAST
Domaini1843 – 190866DRBMAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni256 – 595340Required for interaction with PRKRA and TARBP2By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi175 – 1784DECH box

Sequence similaritiesi

Belongs to the helicase family. Dicer subfamily.Curated
Contains 1 helicase ATP-binding domain.Curated
Contains 1 helicase C-terminal domain.Curated
Contains 1 PAZ domain.Curated
Contains 2 RNase III domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1111.
HOGENOMiHOG000001567.
HOVERGENiHBG107811.
InParanoidiQ8R418.
PhylomeDBiQ8R418.

Family and domain databases

Gene3Di1.10.1520.10. 4 hits.
3.30.160.20. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00104. RNase_III.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR005034. Dicer_dimerisation_dom.
IPR014720. dsRNA-bd_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR003100. PAZ_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF02170. PAZ. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00358. DSRM. 1 hit.
SM00490. HELICc. 1 hit.
SM00949. PAZ. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 4 hits.
PROSITEiPS51327. DICER_DSRBF. 1 hit.
PS50137. DS_RBD. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50821. PAZ. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative promoter usage. Align

Isoform 1 (identifier: Q8R418-1) [UniParc]FASTAAdd to Basket

Also known as: DicerS

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKSPALQPLS MAGLQLMTPA SSPMGPFFGL PWQQEAIHDN IYTPRKYQVE
60 70 80 90 100
LLEAALDHNT IVCLNTGSGK TFIAVLLTKE LAHQIRGDLN PHAKRTVFLV
110 120 130 140 150
NSANQVAQQV SAVRTHSDLK VGEYSDLEVN ASWTKERWSQ EFTKHQVLIM
160 170 180 190 200
TCYVALTVLK NGYLSLSDIN LLVFDECHLA ILDHPYREIM KLCESCPSCP
210 220 230 240 250
RILGLTASIL NGKCDPEELE EKIQKLERIL RSDAETATDL VVLDRYTSQP
260 270 280 290 300
CEIVVDCGPF TDRSGLYERL LMELEAALDF INDCNVAVHS KERDSTLISK
310 320 330 340 350
QILSDCRAVL VVLGPWCADK VAGMMVRELQ KYIKHEQEEL HRKFLLFTDT
360 370 380 390 400
LLRKIHALCE EYFSPASLDL KYVTPKVMKL LEILRKYKPY ERQQFESVEW
410 420 430 440 450
YNNRNQDNYV SWSDSEDDDD DEEIEEKEKP ETNFPSPFTN ILCGIIFVER
460 470 480 490 500
RYTAVVLNRL IKEAGKQDPE LAYISSNFIT GHGIGKNQPR SKQMEAEFRK
510 520 530 540 550
QEEVLRKFRA HETNLLIATS VVEEGVDIPK CNLVVRFDLP TEYRSYVQSK
560 570 580 590 600
GRARAPISNY VMLADTDKIK SFEEDLKTYK AIEKILRNKC SKSADGAEAD
610 620 630 640 650
VHAGVDDEDA FPPYVLRPDD GGPRVTINTA IGHINRYCAR LPSDPFTHLA
660 670 680 690 700
PKCRTRELPD GTFYSTLYLP INSPLRASIV GPPMDSVRLA ERVVALICCE
710 720 730 740 750
KLHKIGELDE HLMPVGKETV KYEEELDLHD EEETSVPGRP GSTKRRQCYP
760 770 780 790 800
KAIPECLRES YPKPDQPCYL YVIGMVLTTP LPDELNFRRR KLYPPEDTTR
810 820 830 840 850
CFGILTAKPI PQIPHFPVYT RSGEVTISIE LKKSGFTLSQ QMLELITRLH
860 870 880 890 900
QYIFSHILRL EKPALEFKPT GAESAYCVLP LNVVNDSGTL DIDFKFMEDI
910 920 930 940 950
EKSEARIGIP STKYSKETPF VFKLEDYQDA VIIPRYRNFD QPHRFYVADV
960 970 980 990 1000
YTDLTPLSKF PSPEYETFAE YYKTKYNLDL TNLNQPLLDV DHTSSRLNLL
1010 1020 1030 1040 1050
TPRHLNQKGK ALPLSSAEKR KAKWESLQNK QILVPELCAI HPIPASLWRK
1060 1070 1080 1090 1100
AVCLPSILYR LHCLLTAEEL RAQTASDAGV GVRSLPVDFR YPNLDFGWKK
1110 1120 1130 1140 1150
SIDSKSFIST CNSSLAESDN YCKHSTTVVP EHAAHQGATR PSLENHDQMS
1160 1170 1180 1190 1200
VNCKRLPAES PAKLQSEVST DLTAINGLSY NKNLANGSYD LVNRDFCQGN
1210 1220 1230 1240 1250
QLNYFKQEIP VQPTTSYPIQ NLYNYENQPK PSNECPLLSN TYLDGNANTS
1260 1270 1280 1290 1300
TSDGSPAVST MPAMMNAVKA LKDRMDSEQS PSVGYSSRTL GPNPGLILQA
1310 1320 1330 1340 1350
LTLSNASDGF NLERLEMLGD SFLKHAITTY LFCTYPDAHE GRLSYMRSKK
1360 1370 1380 1390 1400
VSNCNLYRLG KKKGLPSRMV VSIFDPPVNW LPPGYVVNQD KSNSEKWEKD
1410 1420 1430 1440 1450
EMTKDCLLAN GKLGEACEEE EDLTWRAPKE EAEDEDDFLE YDQEHIQFID
1460 1470 1480 1490 1500
SMLMGSGAFV RKISLSPFSA SDSAYEWKMP KKASLGSMPF ASGLEDFDYS
1510 1520 1530 1540 1550
SWDAMCYLDP SKAVEEDDFV VGFWNPSEEN CGVDTGKQSI SYDLHTEQCI
1560 1570 1580 1590 1600
ADKSIADCVE ALLGCYLTSC GERAAQLFLC SLGLKVLPVI KRTSREKALD
1610 1620 1630 1640 1650
PAQENGSSQQ KSLSGSCAAP VGPRSSAGKD LEYGCLKIPP RCMFDHPDAE
1660 1670 1680 1690 1700
KTLNHLISGF ETFEKKINYR FKNKAYLLQA FTHASYHYNT ITDCYQRLEF
1710 1720 1730 1740 1750
LGDAILDYLI TKHLYEDPRQ HSPGVLTDLR SALVNNTIFA SLAVKYDYHK
1760 1770 1780 1790 1800
YFKAVSPELF HVIDDFVKFQ LEKNEMQGMD SELRRSEEDE EKEEDIEVPK
1810 1820 1830 1840 1850
AMGDIFESLA GAIYMDSGMS LEVVWQVYYP MMQPLIEKFS ANVPRSPVRE
1860 1870 1880 1890 1900
LLEMEPETAK FSPAERTYDG KVRVTVEVVG KGKFKGVGRS YRIAKSAAAR
1910
RALRSLKANQ PQVPNS
Length:1,916
Mass (Da):216,821
Last modified:September 1, 2009 - v3
Checksum:iD97EA17922D7E79C
GO
Isoform 2 (identifier: Q8R418-2) [UniParc]FASTAAdd to Basket

Also known as: DicerO

The sequence of this isoform differs from the canonical sequence as follows:
     1-245: MKSPALQPLS...TATDLVVLDR → MSRDTEV

Note: An MT-C retrotransposon in intron 6 of the mouse DICER gene functions as a promoter producing a transcript lacking exons 1 to 6. A new alternative first exon is directly derived from the retrotransposon.

Show »
Length:1,678
Mass (Da):190,213
Checksum:i4F445FDFB774C3EB
GO

Sequence cautioni

The sequence AAM21495.1 differs from that shown. Reason: Frameshift at position 8.
The sequence BAC15765.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 11M → L in BAC15765. (PubMed:12526743)Curated
Sequence conflicti107 – 1071A → C in AAM21495. (PubMed:15081373)Curated
Sequence conflicti167 – 1671S → P in BAC15765. (PubMed:12526743)Curated
Sequence conflicti289 – 2891H → Y in AAM21495. (PubMed:15081373)Curated
Sequence conflicti610 – 6101A → T in BAC15765. (PubMed:12526743)Curated
Sequence conflicti759 – 7591E → D in AAM21495. (PubMed:15081373)Curated
Sequence conflicti837 – 8371T → I in AAM21495. (PubMed:15081373)Curated
Sequence conflicti888 – 8881G → S in AAL84638. (PubMed:11889553)Curated
Sequence conflicti965 – 9651Y → C in BAC15765. (PubMed:12526743)Curated
Sequence conflicti993 – 9931T → A in BAC15765. (PubMed:12526743)Curated
Sequence conflicti1090 – 10901R → G in BAC15765. (PubMed:12526743)Curated
Sequence conflicti1110 – 11101T → S in BAC15765. (PubMed:12526743)Curated
Sequence conflicti1336 – 13361P → H in AAL84638. (PubMed:11889553)Curated
Sequence conflicti1619 – 16191A → S in AAL84637. (PubMed:11889553)Curated
Sequence conflicti1619 – 16191A → S in BAC15765. (PubMed:12526743)Curated
Sequence conflicti1860 – 18601K → E in BAC15765. (PubMed:12526743)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 245245MKSPA…VVLDR → MSRDTEV in isoform 2. CuratedVSP_053586Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF484523 Genomic DNA. Translation: AAL84637.1.
AF484524 Genomic DNA. Translation: AAL84638.1.
AB081470 mRNA. Translation: BAC15765.1. Different initiation.
AF430845 mRNA. Translation: AAM21495.1. Frameshift.
UniGeneiMm.21135.

Keywords - Coding sequence diversityi

Alternative promoter usage

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF484523 Genomic DNA. Translation: AAL84637.1 .
AF484524 Genomic DNA. Translation: AAL84638.1 .
AB081470 mRNA. Translation: BAC15765.1 . Different initiation.
AF430845 mRNA. Translation: AAM21495.1 . Frameshift.
UniGenei Mm.21135.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3C4B X-ray 1.68 A 1648-1910 [» ]
3C4T X-ray 2.80 A 1648-1910 [» ]
ProteinModelPortali Q8R418.
SMRi Q8R418. Positions 766-1063, 1648-1910.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-29721N.
IntActi Q8R418. 2 interactions.

PTM databases

PhosphoSitei Q8R418.

Proteomic databases

MaxQBi Q8R418.
PaxDbi Q8R418.
PRIDEi Q8R418.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

MGIi MGI:2177178. Dicer1.

Phylogenomic databases

eggNOGi COG1111.
HOGENOMi HOG000001567.
HOVERGENi HBG107811.
InParanoidi Q8R418.
PhylomeDBi Q8R418.

Miscellaneous databases

EvolutionaryTracei Q8R418.
PROi Q8R418.
SOURCEi Search...

Gene expression databases

CleanExi MM_DICER1.
Genevestigatori Q8R418.

Family and domain databases

Gene3Di 1.10.1520.10. 4 hits.
3.30.160.20. 1 hit.
3.40.50.300. 2 hits.
HAMAPi MF_00104. RNase_III.
InterProi IPR011545. DEAD/DEAH_box_helicase_dom.
IPR005034. Dicer_dimerisation_dom.
IPR014720. dsRNA-bd_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR003100. PAZ_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF02170. PAZ. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00358. DSRM. 1 hit.
SM00490. HELICc. 1 hit.
SM00949. PAZ. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view ]
SUPFAMi SSF101690. SSF101690. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 4 hits.
PROSITEi PS51327. DICER_DSRBF. 1 hit.
PS50137. DS_RBD. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50821. PAZ. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of a mouse cDNA encoding Dicer, a ribonuclease III ortholog involved in RNA interference."
    Nicholson R.H., Nicholson A.W.
    Mamm. Genome 13:67-73(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 17-1916.
    Strain: C57BL/6J.
  2. "Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors."
    Doi N., Zenno S., Ueda R., Ohki-Hamazaki H., Ui-Tei K., Saigo K.
    Curr. Biol. 13:41-46(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "RNAi and expression of retrotransposons MuERV-L and IAP in preimplantation mouse embryos."
    Svoboda P., Stein P., Anger M., Bernstein E., Hannon G.J., Schultz R.M.
    Dev. Biol. 269:276-285(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Czech II.
  4. "A retrotransposon-driven dicer isoform directs endogenous small interfering RNA production in mouse oocytes."
    Flemr M., Malik R., Franke V., Nejepinska J., Sedlacek R., Vlahovicek K., Svoboda P.
    Cell 155:807-816(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF ISOFORM 2, FUNCTION (ISOFORMS 1 AND 2), DISRUPTION PHENOTYPE (ISOFORM 2), TISSUE SPECIFICITY (ISOFORMS 1 AND 2).
  5. "Structural and biochemical insights into the dicing mechanism of mouse Dicer: a conserved lysine is critical for dsRNA cleavage."
    Du Z., Lee J.K., Tjhen R., Stroud R.M., James T.L.
    Proc. Natl. Acad. Sci. U.S.A. 105:2391-2396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.68 ANGSTROMS) OF 1647-1910, MUTAGENESIS OF LYS-1800.

Entry informationi

Entry nameiDICER_MOUSE
AccessioniPrimary (citable) accession number: Q8R418
Secondary accession number(s): Q8R419
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: September 1, 2009
Last modified: October 29, 2014
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3