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Q8R418

- DICER_MOUSE

UniProt

Q8R418 - DICER_MOUSE

Protein

Endoribonuclease Dicer

Gene

Dicer1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 3 (01 Sep 2009)
      Previous versions | rss
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    Functioni

    Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes By similarity.By similarity
    Isoform 2: More active than isoform 1 to process long double-stranded RNA into siRNAs. Responsible for the accumulation of endogenous siRNAs observed in mouse oocytes compared to somatic cells and it regulates meiotic spindle organization in female germline.

    Catalytic activityi

    Endonucleolytic cleavage to 5'-phosphomonoester.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi1316 – 13161Magnesium or manganese 1By similarity
    Metal bindingi1395 – 13951Magnesium or manganese 1By similarity
    Metal bindingi1398 – 13981Magnesium or manganese 1By similarity
    Metal bindingi1699 – 16991Magnesium or manganese 2
    Sitei1800 – 18001Important for activity
    Metal bindingi1804 – 18041Magnesium or manganese 2
    Metal bindingi1807 – 18071Magnesium or manganese 2

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi64 – 718ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. helicase activity Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW
    4. miRNA binding Source: MGI
    5. protein binding Source: UniProtKB
    6. ribonuclease III activity Source: UniProtKB

    GO - Biological processi

    1. anatomical structure development Source: MGI
    2. angiogenesis Source: MGI
    3. branching morphogenesis of an epithelial tube Source: MGI
    4. cardiac muscle cell development Source: MGI
    5. cell proliferation Source: MGI
    6. cerebral cortex development Source: MGI
    7. defense response to virus Source: MGI
    8. digestive tract development Source: MGI
    9. embryonic hindlimb morphogenesis Source: MGI
    10. embryonic limb morphogenesis Source: MGI
    11. epidermis morphogenesis Source: MGI
    12. hair follicle cell proliferation Source: MGI
    13. hair follicle development Source: MGI
    14. hair follicle morphogenesis Source: MGI
    15. inner ear receptor cell development Source: MGI
    16. intestinal epithelial cell development Source: MGI
    17. lung development Source: MGI
    18. meiotic spindle organization Source: UniProtKB
    19. mRNA stabilization Source: MGI
    20. multicellular organism growth Source: MGI
    21. negative regulation of Schwann cell proliferation Source: BHF-UCL
    22. negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    23. nerve development Source: BHF-UCL
    24. neuron projection morphogenesis Source: BHF-UCL
    25. olfactory bulb interneuron differentiation Source: MGI
    26. peripheral nervous system myelin formation Source: BHF-UCL
    27. positive regulation of gene expression Source: MGI
    28. positive regulation of miRNA metabolic process Source: MGI
    29. positive regulation of myelination Source: BHF-UCL
    30. positive regulation of Schwann cell differentiation Source: BHF-UCL
    31. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    32. post-embryonic development Source: MGI
    33. pre-miRNA processing Source: UniProtKB
    34. production of miRNAs involved in gene silencing by miRNA Source: BHF-UCL
    35. production of siRNA involved in RNA interference Source: UniProtKB
    36. regulation of cell cycle Source: MGI
    37. regulation of cell differentiation Source: MGI
    38. regulation of enamel mineralization Source: MGI
    39. regulation of gene expression Source: MGI
    40. regulation of miRNA metabolic process Source: MGI
    41. regulation of myelination Source: MGI
    42. regulation of neurogenesis Source: MGI
    43. regulation of neuron differentiation Source: MGI
    44. regulation of Notch signaling pathway Source: BHF-UCL
    45. regulation of odontogenesis of dentin-containing tooth Source: MGI
    46. regulation of oligodendrocyte differentiation Source: MGI
    47. regulation of protein phosphorylation Source: BHF-UCL
    48. regulation of RNA metabolic process Source: MGI
    49. regulation of viral genome replication Source: MGI
    50. reproductive structure development Source: MGI
    51. RNA phosphodiester bond hydrolysis Source: GOC
    52. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
    53. RNA processing Source: MGI
    54. rRNA catabolic process Source: InterPro
    55. spinal cord motor neuron differentiation Source: MGI
    56. spindle assembly Source: MGI
    57. spleen development Source: MGI
    58. stem cell maintenance Source: MGI
    59. targeting of mRNA for destruction involved in RNA interference Source: UniProtKB
    60. zygote asymmetric cell division Source: MGI

    Keywords - Molecular functioni

    Endonuclease, Helicase, Hydrolase, Nuclease

    Keywords - Biological processi

    RNA-mediated gene silencing

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoribonuclease Dicer (EC:3.1.26.3)
    Alternative name(s):
    Double-strand-specific ribonuclease mDCR-1
    Gene namesi
    Name:Dicer1
    Synonyms:Dicer, Mdcr
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:2177178. Dicer1.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. RISC complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Isoform 2: Mice lacking isoform 2 are viable and males are fertile. However, females are sterile, their oocytes displaying meiotic spindle defects.

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi1800 – 18001K → A, R, S or T: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 19161916Endoribonuclease DicerPRO_0000180471Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei413 – 4131PhosphoserineBy similarity
    Modified residuei415 – 4151PhosphoserineBy similarity
    Modified residuei1016 – 10161PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8R418.
    PaxDbiQ8R418.
    PRIDEiQ8R418.

    PTM databases

    PhosphoSiteiQ8R418.

    Expressioni

    Tissue specificityi

    Isoform 1 is expressed in a wide variety of tissues. Isoform 2 is specifically expressed in oocytes during their growth (at protein level).

    Gene expression databases

    CleanExiMM_DICER1.
    GenevestigatoriQ8R418.

    Interactioni

    Subunit structurei

    Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2; DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. Note that the trimeric RLC/miRLC is also referred to as RISC. Interacts with DHX9, AGO1, PIWIL1 and PRKRA. Associates with the 60S ribosome. Interacts with BCDIN3D By similarity.By similarity

    Protein-protein interaction databases

    DIPiDIP-29721N.
    IntActiQ8R418. 2 interactions.

    Structurei

    Secondary structure

    1
    1916
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi1649 – 16568
    Turni1657 – 16593
    Helixi1660 – 16678
    Helixi1674 – 16818
    Helixi1696 – 171621
    Helixi1723 – 173311
    Helixi1736 – 174510
    Helixi1748 – 17503
    Helixi1757 – 177418
    Helixi1800 – 181617
    Helixi1821 – 184222
    Helixi1847 – 18548
    Turni1856 – 18583
    Beta strandi1859 – 18613
    Beta strandi1872 – 18787
    Turni1879 – 18813
    Beta strandi1882 – 18909
    Helixi1891 – 190818

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3C4BX-ray1.68A1648-1910[»]
    3C4TX-ray2.80A1648-1910[»]
    ProteinModelPortaliQ8R418.
    SMRiQ8R418. Positions 766-1063, 1648-1910.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8R418.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini51 – 227177Helicase ATP-bindingAdd
    BLAST
    Domaini433 – 602170Helicase C-terminalAdd
    BLAST
    Domaini630 – 72293Dicer dsRNA-binding foldAdd
    BLAST
    Domaini891 – 1042152PAZAdd
    BLAST
    Domaini1276 – 1403128RNase III 1Add
    BLAST
    Domaini1660 – 1818159RNase III 2Add
    BLAST
    Domaini1843 – 190866DRBMAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni256 – 595340Required for interaction with PRKRA and TARBP2By similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi175 – 1784DECH box

    Sequence similaritiesi

    Belongs to the helicase family. Dicer subfamily.Curated
    Contains 1 helicase ATP-binding domain.Curated
    Contains 1 helicase C-terminal domain.Curated
    Contains 1 PAZ domain.Curated
    Contains 2 RNase III domains.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1111.
    HOGENOMiHOG000001567.
    HOVERGENiHBG107811.
    InParanoidiQ8R419.
    PhylomeDBiQ8R418.

    Family and domain databases

    Gene3Di1.10.1520.10. 4 hits.
    3.30.160.20. 1 hit.
    3.40.50.300. 2 hits.
    HAMAPiMF_00104. RNase_III.
    InterProiIPR005034. Dicer_dimerisation_dom.
    IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014720. dsRNA-bd_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR003100. PAZ_dom.
    IPR011907. RNase_III.
    IPR000999. RNase_III_dom.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF03368. Dicer_dimer. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF02170. PAZ. 1 hit.
    PF00636. Ribonuclease_3. 2 hits.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00358. DSRM. 1 hit.
    SM00490. HELICc. 1 hit.
    SM00949. PAZ. 1 hit.
    SM00535. RIBOc. 2 hits.
    [Graphical view]
    SUPFAMiSSF101690. SSF101690. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF69065. SSF69065. 4 hits.
    PROSITEiPS51327. DICER_DSRBF. 1 hit.
    PS50137. DS_RBD. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS50821. PAZ. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative promoter usage. Align

    Isoform 1 (identifier: Q8R418-1) [UniParc]FASTAAdd to Basket

    Also known as: DicerS

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKSPALQPLS MAGLQLMTPA SSPMGPFFGL PWQQEAIHDN IYTPRKYQVE     50
    LLEAALDHNT IVCLNTGSGK TFIAVLLTKE LAHQIRGDLN PHAKRTVFLV 100
    NSANQVAQQV SAVRTHSDLK VGEYSDLEVN ASWTKERWSQ EFTKHQVLIM 150
    TCYVALTVLK NGYLSLSDIN LLVFDECHLA ILDHPYREIM KLCESCPSCP 200
    RILGLTASIL NGKCDPEELE EKIQKLERIL RSDAETATDL VVLDRYTSQP 250
    CEIVVDCGPF TDRSGLYERL LMELEAALDF INDCNVAVHS KERDSTLISK 300
    QILSDCRAVL VVLGPWCADK VAGMMVRELQ KYIKHEQEEL HRKFLLFTDT 350
    LLRKIHALCE EYFSPASLDL KYVTPKVMKL LEILRKYKPY ERQQFESVEW 400
    YNNRNQDNYV SWSDSEDDDD DEEIEEKEKP ETNFPSPFTN ILCGIIFVER 450
    RYTAVVLNRL IKEAGKQDPE LAYISSNFIT GHGIGKNQPR SKQMEAEFRK 500
    QEEVLRKFRA HETNLLIATS VVEEGVDIPK CNLVVRFDLP TEYRSYVQSK 550
    GRARAPISNY VMLADTDKIK SFEEDLKTYK AIEKILRNKC SKSADGAEAD 600
    VHAGVDDEDA FPPYVLRPDD GGPRVTINTA IGHINRYCAR LPSDPFTHLA 650
    PKCRTRELPD GTFYSTLYLP INSPLRASIV GPPMDSVRLA ERVVALICCE 700
    KLHKIGELDE HLMPVGKETV KYEEELDLHD EEETSVPGRP GSTKRRQCYP 750
    KAIPECLRES YPKPDQPCYL YVIGMVLTTP LPDELNFRRR KLYPPEDTTR 800
    CFGILTAKPI PQIPHFPVYT RSGEVTISIE LKKSGFTLSQ QMLELITRLH 850
    QYIFSHILRL EKPALEFKPT GAESAYCVLP LNVVNDSGTL DIDFKFMEDI 900
    EKSEARIGIP STKYSKETPF VFKLEDYQDA VIIPRYRNFD QPHRFYVADV 950
    YTDLTPLSKF PSPEYETFAE YYKTKYNLDL TNLNQPLLDV DHTSSRLNLL 1000
    TPRHLNQKGK ALPLSSAEKR KAKWESLQNK QILVPELCAI HPIPASLWRK 1050
    AVCLPSILYR LHCLLTAEEL RAQTASDAGV GVRSLPVDFR YPNLDFGWKK 1100
    SIDSKSFIST CNSSLAESDN YCKHSTTVVP EHAAHQGATR PSLENHDQMS 1150
    VNCKRLPAES PAKLQSEVST DLTAINGLSY NKNLANGSYD LVNRDFCQGN 1200
    QLNYFKQEIP VQPTTSYPIQ NLYNYENQPK PSNECPLLSN TYLDGNANTS 1250
    TSDGSPAVST MPAMMNAVKA LKDRMDSEQS PSVGYSSRTL GPNPGLILQA 1300
    LTLSNASDGF NLERLEMLGD SFLKHAITTY LFCTYPDAHE GRLSYMRSKK 1350
    VSNCNLYRLG KKKGLPSRMV VSIFDPPVNW LPPGYVVNQD KSNSEKWEKD 1400
    EMTKDCLLAN GKLGEACEEE EDLTWRAPKE EAEDEDDFLE YDQEHIQFID 1450
    SMLMGSGAFV RKISLSPFSA SDSAYEWKMP KKASLGSMPF ASGLEDFDYS 1500
    SWDAMCYLDP SKAVEEDDFV VGFWNPSEEN CGVDTGKQSI SYDLHTEQCI 1550
    ADKSIADCVE ALLGCYLTSC GERAAQLFLC SLGLKVLPVI KRTSREKALD 1600
    PAQENGSSQQ KSLSGSCAAP VGPRSSAGKD LEYGCLKIPP RCMFDHPDAE 1650
    KTLNHLISGF ETFEKKINYR FKNKAYLLQA FTHASYHYNT ITDCYQRLEF 1700
    LGDAILDYLI TKHLYEDPRQ HSPGVLTDLR SALVNNTIFA SLAVKYDYHK 1750
    YFKAVSPELF HVIDDFVKFQ LEKNEMQGMD SELRRSEEDE EKEEDIEVPK 1800
    AMGDIFESLA GAIYMDSGMS LEVVWQVYYP MMQPLIEKFS ANVPRSPVRE 1850
    LLEMEPETAK FSPAERTYDG KVRVTVEVVG KGKFKGVGRS YRIAKSAAAR 1900
    RALRSLKANQ PQVPNS 1916
    Length:1,916
    Mass (Da):216,821
    Last modified:September 1, 2009 - v3
    Checksum:iD97EA17922D7E79C
    GO
    Isoform 2 (identifier: Q8R418-2) [UniParc]FASTAAdd to Basket

    Also known as: DicerO

    The sequence of this isoform differs from the canonical sequence as follows:
         1-245: MKSPALQPLS...TATDLVVLDR → MSRDTEV

    Note: An MT-C retrotransposon in intron 6 of the mouse DICER gene functions as a promoter producing a transcript lacking exons 1 to 6. A new alternative first exon is directly derived from the retrotransposon.

    Show »
    Length:1,678
    Mass (Da):190,213
    Checksum:i4F445FDFB774C3EB
    GO

    Sequence cautioni

    The sequence AAM21495.1 differs from that shown. Reason: Frameshift at position 8.
    The sequence BAC15765.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1 – 11M → L in BAC15765. (PubMed:12526743)Curated
    Sequence conflicti107 – 1071A → C in AAM21495. (PubMed:15081373)Curated
    Sequence conflicti167 – 1671S → P in BAC15765. (PubMed:12526743)Curated
    Sequence conflicti289 – 2891H → Y in AAM21495. (PubMed:15081373)Curated
    Sequence conflicti610 – 6101A → T in BAC15765. (PubMed:12526743)Curated
    Sequence conflicti759 – 7591E → D in AAM21495. (PubMed:15081373)Curated
    Sequence conflicti837 – 8371T → I in AAM21495. (PubMed:15081373)Curated
    Sequence conflicti888 – 8881G → S in AAL84638. (PubMed:11889553)Curated
    Sequence conflicti965 – 9651Y → C in BAC15765. (PubMed:12526743)Curated
    Sequence conflicti993 – 9931T → A in BAC15765. (PubMed:12526743)Curated
    Sequence conflicti1090 – 10901R → G in BAC15765. (PubMed:12526743)Curated
    Sequence conflicti1110 – 11101T → S in BAC15765. (PubMed:12526743)Curated
    Sequence conflicti1336 – 13361P → H in AAL84638. (PubMed:11889553)Curated
    Sequence conflicti1619 – 16191A → S in AAL84637. (PubMed:11889553)Curated
    Sequence conflicti1619 – 16191A → S in BAC15765. (PubMed:12526743)Curated
    Sequence conflicti1860 – 18601K → E in BAC15765. (PubMed:12526743)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 245245MKSPA…VVLDR → MSRDTEV in isoform 2. CuratedVSP_053586Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF484523 Genomic DNA. Translation: AAL84637.1.
    AF484524 Genomic DNA. Translation: AAL84638.1.
    AB081470 mRNA. Translation: BAC15765.1. Different initiation.
    AF430845 mRNA. Translation: AAM21495.1. Frameshift.
    UniGeneiMm.21135.

    Keywords - Coding sequence diversityi

    Alternative promoter usage

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF484523 Genomic DNA. Translation: AAL84637.1 .
    AF484524 Genomic DNA. Translation: AAL84638.1 .
    AB081470 mRNA. Translation: BAC15765.1 . Different initiation.
    AF430845 mRNA. Translation: AAM21495.1 . Frameshift.
    UniGenei Mm.21135.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3C4B X-ray 1.68 A 1648-1910 [» ]
    3C4T X-ray 2.80 A 1648-1910 [» ]
    ProteinModelPortali Q8R418.
    SMRi Q8R418. Positions 766-1063, 1648-1910.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-29721N.
    IntActi Q8R418. 2 interactions.

    PTM databases

    PhosphoSitei Q8R418.

    Proteomic databases

    MaxQBi Q8R418.
    PaxDbi Q8R418.
    PRIDEi Q8R418.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:2177178. Dicer1.

    Phylogenomic databases

    eggNOGi COG1111.
    HOGENOMi HOG000001567.
    HOVERGENi HBG107811.
    InParanoidi Q8R419.
    PhylomeDBi Q8R418.

    Miscellaneous databases

    EvolutionaryTracei Q8R418.
    PROi Q8R418.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_DICER1.
    Genevestigatori Q8R418.

    Family and domain databases

    Gene3Di 1.10.1520.10. 4 hits.
    3.30.160.20. 1 hit.
    3.40.50.300. 2 hits.
    HAMAPi MF_00104. RNase_III.
    InterProi IPR005034. Dicer_dimerisation_dom.
    IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014720. dsRNA-bd_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR003100. PAZ_dom.
    IPR011907. RNase_III.
    IPR000999. RNase_III_dom.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF03368. Dicer_dimer. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF02170. PAZ. 1 hit.
    PF00636. Ribonuclease_3. 2 hits.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00358. DSRM. 1 hit.
    SM00490. HELICc. 1 hit.
    SM00949. PAZ. 1 hit.
    SM00535. RIBOc. 2 hits.
    [Graphical view ]
    SUPFAMi SSF101690. SSF101690. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF69065. SSF69065. 4 hits.
    PROSITEi PS51327. DICER_DSRBF. 1 hit.
    PS50137. DS_RBD. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS50821. PAZ. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of a mouse cDNA encoding Dicer, a ribonuclease III ortholog involved in RNA interference."
      Nicholson R.H., Nicholson A.W.
      Mamm. Genome 13:67-73(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 17-1916.
      Strain: C57BL/6J.
    2. "Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors."
      Doi N., Zenno S., Ueda R., Ohki-Hamazaki H., Ui-Tei K., Saigo K.
      Curr. Biol. 13:41-46(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    3. "RNAi and expression of retrotransposons MuERV-L and IAP in preimplantation mouse embryos."
      Svoboda P., Stein P., Anger M., Bernstein E., Hannon G.J., Schultz R.M.
      Dev. Biol. 269:276-285(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: Czech II.
    4. "A retrotransposon-driven dicer isoform directs endogenous small interfering RNA production in mouse oocytes."
      Flemr M., Malik R., Franke V., Nejepinska J., Sedlacek R., Vlahovicek K., Svoboda P.
      Cell 155:807-816(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF ISOFORM 2, FUNCTION (ISOFORMS 1 AND 2), DISRUPTION PHENOTYPE (ISOFORM 2), TISSUE SPECIFICITY (ISOFORMS 1 AND 2).
    5. "Structural and biochemical insights into the dicing mechanism of mouse Dicer: a conserved lysine is critical for dsRNA cleavage."
      Du Z., Lee J.K., Tjhen R., Stroud R.M., James T.L.
      Proc. Natl. Acad. Sci. U.S.A. 105:2391-2396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.68 ANGSTROMS) OF 1647-1910, MUTAGENESIS OF LYS-1800.

    Entry informationi

    Entry nameiDICER_MOUSE
    AccessioniPrimary (citable) accession number: Q8R418
    Secondary accession number(s): Q8R419
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2002
    Last sequence update: September 1, 2009
    Last modified: October 1, 2014
    This is version 121 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3