Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8R409

- HEXI1_MOUSE

UniProt

Q8R409 - HEXI1_MOUSE

Protein

Protein HEXIM1

Gene

Hexim1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity By similarity.By similarity

    GO - Molecular functioni

    1. cyclin-dependent protein serine/threonine kinase inhibitor activity Source: UniProtKB
    2. protein binding Source: IntAct
    3. snRNA binding Source: UniProtKB

    GO - Biological processi

    1. heart development Source: MGI
    2. negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
    3. negative regulation of transcription, DNA-templated Source: UniProtKB
    4. negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein HEXIM1
    Alternative name(s):
    Cardiac lineage protein 1
    Gene namesi
    Name:Hexim1
    Synonyms:Clp1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:2385923. Hexim1.

    Subcellular locationi

    Nucleus By similarity. Cytoplasm By similarity
    Note: Binds alpha-importin and is mostly nuclear.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 356356Protein HEXIM1PRO_0000305264Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei98 – 981PhosphoserineBy similarity
    Modified residuei230 – 2301PhosphoserineBy similarity
    Modified residuei233 – 2331PhosphothreonineBy similarity
    Modified residuei234 – 2341PhosphoserineBy similarity
    Modified residuei249 – 2491PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8R409.
    PaxDbiQ8R409.
    PRIDEiQ8R409.

    PTM databases

    PhosphoSiteiQ8R409.

    Expressioni

    Tissue specificityi

    Widely expressed with higher expression in heart, skeletal muscle and brain (at protein level).1 Publication

    Inductioni

    Up-regulated by HMBA (hexamethylene bisacetamide).1 Publication

    Gene expression databases

    ArrayExpressiQ8R409.
    BgeeiQ8R409.
    CleanExiMM_HEXIM1.
    GenevestigatoriQ8R409.

    Interactioni

    Subunit structurei

    Homooligomer and heterooligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Srsf1Q6PDM24EBI-6261031,EBI-2550360
    Srsf2Q620934EBI-6261031,EBI-2550402

    Protein-protein interaction databases

    IntActiQ8R409. 4 interactions.
    MINTiMINT-4119070.
    STRINGi10090.ENSMUSP00000057339.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8R409.
    SMRiQ8R409. Positions 251-356.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni147 – 17428Basic region; mediates nuclear localization and interaction with 7SK snRNA and NR3C1By similarityAdd
    BLAST
    Regioni199 – 2024Interaction with P-TEFbBy similarity
    Regioni207 – 24741Autoinhibitory acidic region; in absence of 7SK snRNA interacts with the basic region preventing interaction with P-TEFb and modulating subcellular localizationBy similarityAdd
    BLAST
    Regioni283 – 31129Mediates interaction with CCNT1By similarityAdd
    BLAST
    Regioni307 – 35246Required for inhibition of ESR1-dependent transcriptionBy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili280 – 34667Sequence AnalysisAdd
    BLAST

    Domaini

    The coiled-coil domain mediates oligomerization.By similarity

    Sequence similaritiesi

    Belongs to the HEXIM family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG72325.
    GeneTreeiENSGT00390000002808.
    HOGENOMiHOG000060338.
    HOVERGENiHBG053249.
    InParanoidiQ8R409.
    KOiK15189.
    OMAiKGQNGDD.
    OrthoDBiEOG771281.
    PhylomeDBiQ8R409.
    TreeFamiTF336851.

    Family and domain databases

    InterProiIPR024872. HEXIM.
    [Graphical view]
    PANTHERiPTHR13469. PTHR13469. 1 hit.
    PfamiPF15313. HEXIM. 1 hit.
    [Graphical view]
    PRINTSiPR02094. HEXIMFAMILY.

    Sequencei

    Sequence statusi: Complete.

    Q8R409-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEPLLTEHQ HQPQTSNCTG AAVVHEEHTS ERPPSAEERV PKEDSRWQSR    50
    ASLQSGSRPG QEGEGGLKHQ LPPLQTNACP ELSSLEKGEK GQNGEDLSTG 100
    GASPSAEGEP MSESLVQPGH DSEATKQEAP AAGGEEPWGQ QQRQLGKKKH 150
    RRRPSKKKRH WKPYYKLTWE EKKKFDEKQS LRASRVRAEM FAKGQPVAPY 200
    NTTQFLMDDH DQEEPDLKTG LYPKRAAAKS DDTSDEDFVE EAGEEDGGSD 250
    GMGGDGSEFL QRDFSETYER YHAESLQNMS KQELIKEYLE LEKCLSRKED 300
    ENNRLRLESK RLGGVDARVR ELELELDRLR AENLQLLTEN ELHRQQERAP 350
    LSKFGD 356
    Length:356
    Mass (Da):40,243
    Last modified:June 1, 2002 - v1
    Checksum:i242DE7EE66BA293B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY090614 mRNA. Translation: AAM09026.1.
    AK134535 mRNA. Translation: BAE22173.1.
    AL731805 Genomic DNA. Translation: CAM22092.1.
    CCDSiCCDS25513.1.
    RefSeqiNP_620092.1. NM_138753.2.
    UniGeneiMm.8763.

    Genome annotation databases

    EnsembliENSMUST00000053063; ENSMUSP00000057339; ENSMUSG00000048878.
    GeneIDi192231.
    KEGGimmu:192231.
    UCSCiuc007lto.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY090614 mRNA. Translation: AAM09026.1 .
    AK134535 mRNA. Translation: BAE22173.1 .
    AL731805 Genomic DNA. Translation: CAM22092.1 .
    CCDSi CCDS25513.1.
    RefSeqi NP_620092.1. NM_138753.2.
    UniGenei Mm.8763.

    3D structure databases

    ProteinModelPortali Q8R409.
    SMRi Q8R409. Positions 251-356.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8R409. 4 interactions.
    MINTi MINT-4119070.
    STRINGi 10090.ENSMUSP00000057339.

    PTM databases

    PhosphoSitei Q8R409.

    Proteomic databases

    MaxQBi Q8R409.
    PaxDbi Q8R409.
    PRIDEi Q8R409.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000053063 ; ENSMUSP00000057339 ; ENSMUSG00000048878 .
    GeneIDi 192231.
    KEGGi mmu:192231.
    UCSCi uc007lto.1. mouse.

    Organism-specific databases

    CTDi 10614.
    MGIi MGI:2385923. Hexim1.

    Phylogenomic databases

    eggNOGi NOG72325.
    GeneTreei ENSGT00390000002808.
    HOGENOMi HOG000060338.
    HOVERGENi HBG053249.
    InParanoidi Q8R409.
    KOi K15189.
    OMAi KGQNGDD.
    OrthoDBi EOG771281.
    PhylomeDBi Q8R409.
    TreeFami TF336851.

    Miscellaneous databases

    ChiTaRSi HEXIM1. mouse.
    NextBioi 371270.
    PROi Q8R409.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8R409.
    Bgeei Q8R409.
    CleanExi MM_HEXIM1.
    Genevestigatori Q8R409.

    Family and domain databases

    InterProi IPR024872. HEXIM.
    [Graphical view ]
    PANTHERi PTHR13469. PTHR13469. 1 hit.
    Pfami PF15313. HEXIM. 1 hit.
    [Graphical view ]
    PRINTSi PR02094. HEXIMFAMILY.
    ProtoNeti Search...

    Publicationsi

    1. "Structure, expression, and functional characterization of the mouse CLP-1 gene."
      Huang F., Wagner M., Siddiqui M.A.Q.
      Gene 292:245-259(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
      Strain: 129/SvJ.
      Tissue: Embryonic heart.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Medulla oblongata.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiHEXI1_MOUSE
    AccessioniPrimary (citable) accession number: Q8R409
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3