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Q8R409

- HEXI1_MOUSE

UniProt

Q8R409 - HEXI1_MOUSE

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Protein

Protein HEXIM1

Gene
Hexim1, Clp1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity By similarity.

GO - Molecular functioni

  1. cyclin-dependent protein serine/threonine kinase inhibitor activity Source: UniProtKB
  2. protein binding Source: IntAct
  3. snRNA binding Source: UniProtKB

GO - Biological processi

  1. heart development Source: MGI
  2. negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
  3. negative regulation of transcription, DNA-templated Source: UniProtKB
  4. negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein HEXIM1
Alternative name(s):
Cardiac lineage protein 1
Gene namesi
Name:Hexim1
Synonyms:Clp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:2385923. Hexim1.

Subcellular locationi

Nucleus By similarity. Cytoplasm By similarity
Note: Binds alpha-importin and is mostly nuclear By similarity.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 356356Protein HEXIM1PRO_0000305264Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei98 – 981Phosphoserine By similarity
Modified residuei230 – 2301Phosphoserine By similarity
Modified residuei233 – 2331Phosphothreonine By similarity
Modified residuei234 – 2341Phosphoserine By similarity
Modified residuei249 – 2491Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8R409.
PaxDbiQ8R409.
PRIDEiQ8R409.

PTM databases

PhosphoSiteiQ8R409.

Expressioni

Tissue specificityi

Widely expressed with higher expression in heart, skeletal muscle and brain (at protein level).1 Publication

Inductioni

Up-regulated by HMBA (hexamethylene bisacetamide).1 Publication

Gene expression databases

ArrayExpressiQ8R409.
BgeeiQ8R409.
CleanExiMM_HEXIM1.
GenevestigatoriQ8R409.

Interactioni

Subunit structurei

Homooligomer and heterooligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
Srsf1Q6PDM24EBI-6261031,EBI-2550360
Srsf2Q620934EBI-6261031,EBI-2550402

Protein-protein interaction databases

IntActiQ8R409. 4 interactions.
MINTiMINT-4119070.
STRINGi10090.ENSMUSP00000057339.

Structurei

3D structure databases

ProteinModelPortaliQ8R409.
SMRiQ8R409. Positions 251-356.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni147 – 17428Basic region; mediates nuclear localization and interaction with 7SK snRNA and NR3C1 By similarityAdd
BLAST
Regioni199 – 2024Interaction with P-TEFb By similarity
Regioni207 – 24741Autoinhibitory acidic region; in absence of 7SK snRNA interacts with the basic region preventing interaction with P-TEFb and modulating subcellular localization By similarityAdd
BLAST
Regioni283 – 31129Mediates interaction with CCNT1 By similarityAdd
BLAST
Regioni307 – 35246Required for inhibition of ESR1-dependent transcription By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili280 – 34667 Reviewed predictionAdd
BLAST

Domaini

The coiled-coil domain mediates oligomerization By similarity.

Sequence similaritiesi

Belongs to the HEXIM family.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG72325.
GeneTreeiENSGT00390000002808.
HOGENOMiHOG000060338.
HOVERGENiHBG053249.
InParanoidiQ8R409.
KOiK15189.
OMAiKGQNGDD.
OrthoDBiEOG771281.
PhylomeDBiQ8R409.
TreeFamiTF336851.

Family and domain databases

InterProiIPR024872. HEXIM.
[Graphical view]
PANTHERiPTHR13469. PTHR13469. 1 hit.
PfamiPF15313. HEXIM. 1 hit.
[Graphical view]
PRINTSiPR02094. HEXIMFAMILY.

Sequencei

Sequence statusi: Complete.

Q8R409-1 [UniParc]FASTAAdd to Basket

« Hide

MAEPLLTEHQ HQPQTSNCTG AAVVHEEHTS ERPPSAEERV PKEDSRWQSR    50
ASLQSGSRPG QEGEGGLKHQ LPPLQTNACP ELSSLEKGEK GQNGEDLSTG 100
GASPSAEGEP MSESLVQPGH DSEATKQEAP AAGGEEPWGQ QQRQLGKKKH 150
RRRPSKKKRH WKPYYKLTWE EKKKFDEKQS LRASRVRAEM FAKGQPVAPY 200
NTTQFLMDDH DQEEPDLKTG LYPKRAAAKS DDTSDEDFVE EAGEEDGGSD 250
GMGGDGSEFL QRDFSETYER YHAESLQNMS KQELIKEYLE LEKCLSRKED 300
ENNRLRLESK RLGGVDARVR ELELELDRLR AENLQLLTEN ELHRQQERAP 350
LSKFGD 356
Length:356
Mass (Da):40,243
Last modified:June 1, 2002 - v1
Checksum:i242DE7EE66BA293B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY090614 mRNA. Translation: AAM09026.1.
AK134535 mRNA. Translation: BAE22173.1.
AL731805 Genomic DNA. Translation: CAM22092.1.
CCDSiCCDS25513.1.
RefSeqiNP_620092.1. NM_138753.2.
UniGeneiMm.8763.

Genome annotation databases

EnsembliENSMUST00000053063; ENSMUSP00000057339; ENSMUSG00000048878.
GeneIDi192231.
KEGGimmu:192231.
UCSCiuc007lto.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY090614 mRNA. Translation: AAM09026.1 .
AK134535 mRNA. Translation: BAE22173.1 .
AL731805 Genomic DNA. Translation: CAM22092.1 .
CCDSi CCDS25513.1.
RefSeqi NP_620092.1. NM_138753.2.
UniGenei Mm.8763.

3D structure databases

ProteinModelPortali Q8R409.
SMRi Q8R409. Positions 251-356.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8R409. 4 interactions.
MINTi MINT-4119070.
STRINGi 10090.ENSMUSP00000057339.

PTM databases

PhosphoSitei Q8R409.

Proteomic databases

MaxQBi Q8R409.
PaxDbi Q8R409.
PRIDEi Q8R409.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000053063 ; ENSMUSP00000057339 ; ENSMUSG00000048878 .
GeneIDi 192231.
KEGGi mmu:192231.
UCSCi uc007lto.1. mouse.

Organism-specific databases

CTDi 10614.
MGIi MGI:2385923. Hexim1.

Phylogenomic databases

eggNOGi NOG72325.
GeneTreei ENSGT00390000002808.
HOGENOMi HOG000060338.
HOVERGENi HBG053249.
InParanoidi Q8R409.
KOi K15189.
OMAi KGQNGDD.
OrthoDBi EOG771281.
PhylomeDBi Q8R409.
TreeFami TF336851.

Miscellaneous databases

ChiTaRSi HEXIM1. mouse.
NextBioi 371270.
PROi Q8R409.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8R409.
Bgeei Q8R409.
CleanExi MM_HEXIM1.
Genevestigatori Q8R409.

Family and domain databases

InterProi IPR024872. HEXIM.
[Graphical view ]
PANTHERi PTHR13469. PTHR13469. 1 hit.
Pfami PF15313. HEXIM. 1 hit.
[Graphical view ]
PRINTSi PR02094. HEXIMFAMILY.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure, expression, and functional characterization of the mouse CLP-1 gene."
    Huang F., Wagner M., Siddiqui M.A.Q.
    Gene 292:245-259(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
    Strain: 129/SvJ.
    Tissue: Embryonic heart.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Medulla oblongata.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiHEXI1_MOUSE
AccessioniPrimary (citable) accession number: Q8R409
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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