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Q8R409 (HEXI1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein HEXIM1
Alternative name(s):
Cardiac lineage protein 1
Gene names
Name:Hexim1
Synonyms:Clp1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity By similarity.

Subunit structure

Homooligomer and heterooligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA By similarity.

Subcellular location

Nucleus By similarity. Cytoplasm By similarity. Note: Binds alpha-importin and is mostly nuclear By similarity.

Tissue specificity

Widely expressed with higher expression in heart, skeletal muscle and brain (at protein level). Ref.1

Induction

Up-regulated by HMBA (hexamethylene bisacetamide). Ref.1

Domain

The coiled-coil domain mediates oligomerization By similarity.

Sequence similarities

Belongs to the HEXIM family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 356356Protein HEXIM1
PRO_0000305264

Regions

Region147 – 17428Basic region; mediates nuclear localization and interaction with 7SK snRNA and NR3C1 By similarity
Region199 – 2024Interaction with P-TEFb By similarity
Region207 – 24741Autoinhibitory acidic region; in absence of 7SK snRNA interacts with the basic region preventing interaction with P-TEFb and modulating subcellular localization By similarity
Region283 – 31129Mediates interaction with CCNT1 By similarity
Region307 – 35246Required for inhibition of ESR1-dependent transcription By similarity
Coiled coil280 – 34667 Potential

Amino acid modifications

Modified residue981Phosphoserine By similarity
Modified residue2301Phosphoserine By similarity
Modified residue2331Phosphothreonine By similarity
Modified residue2341Phosphoserine By similarity
Modified residue2491Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8R409 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 242DE7EE66BA293B

FASTA35640,243
        10         20         30         40         50         60 
MAEPLLTEHQ HQPQTSNCTG AAVVHEEHTS ERPPSAEERV PKEDSRWQSR ASLQSGSRPG 

        70         80         90        100        110        120 
QEGEGGLKHQ LPPLQTNACP ELSSLEKGEK GQNGEDLSTG GASPSAEGEP MSESLVQPGH 

       130        140        150        160        170        180 
DSEATKQEAP AAGGEEPWGQ QQRQLGKKKH RRRPSKKKRH WKPYYKLTWE EKKKFDEKQS 

       190        200        210        220        230        240 
LRASRVRAEM FAKGQPVAPY NTTQFLMDDH DQEEPDLKTG LYPKRAAAKS DDTSDEDFVE 

       250        260        270        280        290        300 
EAGEEDGGSD GMGGDGSEFL QRDFSETYER YHAESLQNMS KQELIKEYLE LEKCLSRKED 

       310        320        330        340        350 
ENNRLRLESK RLGGVDARVR ELELELDRLR AENLQLLTEN ELHRQQERAP LSKFGD 

« Hide

References

« Hide 'large scale' references
[1]"Structure, expression, and functional characterization of the mouse CLP-1 gene."
Huang F., Wagner M., Siddiqui M.A.Q.
Gene 292:245-259(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
Strain: 129/SvJ.
Tissue: Embryonic heart.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Medulla oblongata.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY090614 mRNA. Translation: AAM09026.1.
AK134535 mRNA. Translation: BAE22173.1.
AL731805 Genomic DNA. Translation: CAM22092.1.
CCDSCCDS25513.1.
RefSeqNP_620092.1. NM_138753.2.
UniGeneMm.8763.

3D structure databases

ProteinModelPortalQ8R409.
SMRQ8R409. Positions 251-356.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8R409. 4 interactions.
MINTMINT-4119070.
STRING10090.ENSMUSP00000057339.

PTM databases

PhosphoSiteQ8R409.

Proteomic databases

MaxQBQ8R409.
PaxDbQ8R409.
PRIDEQ8R409.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000053063; ENSMUSP00000057339; ENSMUSG00000048878.
GeneID192231.
KEGGmmu:192231.
UCSCuc007lto.1. mouse.

Organism-specific databases

CTD10614.
MGIMGI:2385923. Hexim1.

Phylogenomic databases

eggNOGNOG72325.
GeneTreeENSGT00390000002808.
HOGENOMHOG000060338.
HOVERGENHBG053249.
InParanoidQ8R409.
KOK15189.
OMAKGQNGDD.
OrthoDBEOG771281.
PhylomeDBQ8R409.
TreeFamTF336851.

Gene expression databases

ArrayExpressQ8R409.
BgeeQ8R409.
CleanExMM_HEXIM1.
GenevestigatorQ8R409.

Family and domain databases

InterProIPR024872. HEXIM.
[Graphical view]
PANTHERPTHR13469. PTHR13469. 1 hit.
PfamPF15313. HEXIM. 1 hit.
[Graphical view]
PRINTSPR02094. HEXIMFAMILY.
ProtoNetSearch...

Other

ChiTaRSHEXIM1. mouse.
NextBio371270.
PROQ8R409.
SOURCESearch...

Entry information

Entry nameHEXI1_MOUSE
AccessionPrimary (citable) accession number: Q8R409
Entry history
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot