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Protein

Anterior gradient protein 3

Gene

Agr3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Required for calcium-mediated regulation of ciliary beat frequency and mucociliary clearance in the airway. Might be involved in the regulation of intracellular calcium in tracheal epithelial cells.1 Publication

GO - Molecular functioni

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Anterior gradient protein 3Imported
Gene namesi
Name:Agr3Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2685734. Agr3.

Subcellular locationi

  • Endoplasmic reticulum By similarity
  • Cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

Knockout mice develop ciliated cells with normal-appearing cilia and histology. However, ciliary beat frequency was lower in airways from knockout mice compared with control mice in presence of calcium (20% lower in the absence of stimulation, 35% lower after ATP stimulation). Knockout mice show reduced mucociliary clearance. Knockout mice do not seem to show endoplasmic reticulum stress response.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 165145Anterior gradient protein 3PRO_0000001041Add
BLAST

Proteomic databases

MaxQBiQ8R3W7.
PaxDbiQ8R3W7.
PRIDEiQ8R3W7.

Expressioni

Tissue specificityi

Expressed in the ciliated cells of the airway epithelium. Not detected in the mucous cells.1 Publication

Gene expression databases

BgeeiQ8R3W7.
CleanExiMM_AGR3.
ExpressionAtlasiQ8R3W7. baseline and differential.
GenevisibleiQ8R3W7. MM.

Interactioni

Subunit structurei

Interacts with LYPD3 and DAG1 (alphaDAG1).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049212.

Structurei

3D structure databases

ProteinModelPortaliQ8R3W7.
SMRiQ8R3W7. Positions 30-164.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi162 – 1654Prevents secretion from ERCurated

Sequence similaritiesi

Belongs to the AGR family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IWAH. Eukaryota.
ENOG4111K70. LUCA.
GeneTreeiENSGT00530000063273.
HOGENOMiHOG000231100.
HOVERGENiHBG006516.
InParanoidiQ8R3W7.
OMAiGDEITWV.
OrthoDBiEOG7DNNX2.
PhylomeDBiQ8R3W7.
TreeFamiTF321449.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR012336. Thioredoxin-like_fold.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R3W7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLHSALALCL LLITVSSNLA IAIKKEKRPP QTLSRGWGDD ITWVQTYEEG
60 70 80 90 100
LFHARKSNKP LMVIHHLEDC QYCQALKKEF AKNEEIQEMA QNDFIMLNLM
110 120 130 140 150
HETTDKNLSP DGQYVPRIMF VDPSLTVRAD ITGRYSNRLY TYEPQDLPML
160
VDNMKKALRL IQSEL
Length:165
Mass (Da):19,081
Last modified:June 1, 2002 - v1
Checksum:i39FBC7DCB36E122C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti90 – 901A → E in BAC35297 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK053177 mRNA. Translation: BAC35297.1.
CT030196 Genomic DNA. Translation: CAO91839.1.
BC023499 mRNA. Translation: AAH23499.1.
CCDSiCCDS25880.1.
RefSeqiNP_997414.2. NM_207531.3.
UniGeneiMm.190508.

Genome annotation databases

EnsembliENSMUST00000042101; ENSMUSP00000049212; ENSMUSG00000036231.
GeneIDi403205.
KEGGimmu:403205.
UCSCiuc007njl.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK053177 mRNA. Translation: BAC35297.1.
CT030196 Genomic DNA. Translation: CAO91839.1.
BC023499 mRNA. Translation: AAH23499.1.
CCDSiCCDS25880.1.
RefSeqiNP_997414.2. NM_207531.3.
UniGeneiMm.190508.

3D structure databases

ProteinModelPortaliQ8R3W7.
SMRiQ8R3W7. Positions 30-164.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049212.

Proteomic databases

MaxQBiQ8R3W7.
PaxDbiQ8R3W7.
PRIDEiQ8R3W7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042101; ENSMUSP00000049212; ENSMUSG00000036231.
GeneIDi403205.
KEGGimmu:403205.
UCSCiuc007njl.2. mouse.

Organism-specific databases

CTDi155465.
MGIiMGI:2685734. Agr3.

Phylogenomic databases

eggNOGiENOG410IWAH. Eukaryota.
ENOG4111K70. LUCA.
GeneTreeiENSGT00530000063273.
HOGENOMiHOG000231100.
HOVERGENiHBG006516.
InParanoidiQ8R3W7.
OMAiGDEITWV.
OrthoDBiEOG7DNNX2.
PhylomeDBiQ8R3W7.
TreeFamiTF321449.

Miscellaneous databases

PROiQ8R3W7.
SOURCEiSearch...

Gene expression databases

BgeeiQ8R3W7.
CleanExiMM_AGR3.
ExpressionAtlasiQ8R3W7. baseline and differential.
GenevisibleiQ8R3W7. MM.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR012336. Thioredoxin-like_fold.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Lung.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N-3.
    Tissue: Mammary tumor.
  4. "The ER resident protein AGR3 is required for regulation of ciliary beat frequency in the airway."
    Bonser L.R., Schroeder B.W., Ostrin L.A., Baumlin N., Olson J.L., Salathe M., Erle D.J.
    Am. J. Respir. Cell Mol. Biol. 53:536-543(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, FUNCTION.

Entry informationi

Entry nameiAGR3_MOUSE
AccessioniPrimary (citable) accession number: Q8R3W7
Secondary accession number(s): A7NSI1, Q8BW95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: June 1, 2002
Last modified: June 8, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.