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Q8R3W5

- SEN15_MOUSE

UniProt

Q8R3W5 - SEN15_MOUSE

Protein

tRNA-splicing endonuclease subunit Sen15

Gene

Tsen15

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events By similarity.By similarity

    GO - Molecular functioni

    1. tRNA-intron endonuclease activity Source: InterPro

    GO - Biological processi

    1. mRNA processing Source: UniProtKB-KW
    2. tRNA splicing, via endonucleolytic cleavage and ligation Source: InterPro

    Keywords - Biological processi

    mRNA processing, tRNA processing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-splicing endonuclease subunit Sen15
    Alternative name(s):
    tRNA-intron endonuclease Sen15
    Gene namesi
    Name:Tsen15
    Synonyms:Sen15
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:1913887. Tsen15.

    Subcellular locationi

    Nucleus Curated. Nucleusnucleolus Curated
    Note: May be transiently localized in the nucleolus.Curated

    GO - Cellular componenti

    1. nucleolus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 168168tRNA-splicing endonuclease subunit Sen15PRO_0000194024Add
    BLAST

    Proteomic databases

    PaxDbiQ8R3W5.
    PRIDEiQ8R3W5.

    Expressioni

    Gene expression databases

    GenevestigatoriQ8R3W5.

    Interactioni

    Subunit structurei

    Homodimer. tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34/LENG5 and SEN54. tRNA splicing endonuclease complex also contains proteins of the pre-mRNA 3' end processing machinery such as CLP1, CPSF1, CPSF4 and CSTF2 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi211612. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8R3W5.
    SMRiQ8R3W5. Positions 33-154.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the SEN15 family.Curated

    Phylogenomic databases

    eggNOGiNOG44101.
    HOGENOMiHOG000290172.
    HOVERGENiHBG058504.
    InParanoidiA6H601.
    KOiK15324.
    PhylomeDBiQ8R3W5.

    Family and domain databases

    InterProiIPR018593. tRNA-endonuc_su_Sen15.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    [Graphical view]
    PfamiPF09631. Sen15. 1 hit.
    [Graphical view]
    SUPFAMiSSF53032. SSF53032. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8R3W5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEERSDSEPT PGCSGPGPAP VRDGGGAHTW APEDAWMGTH PKYLEMMELD    50
    IGDATQVYIA FLVYLDLMES KSWHEVNCVG IPELQLICLL GTEIEGEGLQ 100
    TVVPTPISAS LSHNRIREIL KASRKLQGDP ELPMSFTLAI VESDSTIVYY 150
    KLTDGFMLPD PQNISLRR 168
    Length:168
    Mass (Da):18,530
    Last modified:June 1, 2002 - v1
    Checksum:i99D8E6E14C2FCAA0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti5 – 84SDSE → TSSK in BAB30855. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK017650 mRNA. Translation: BAB30855.1.
    BC024342 mRNA. Translation: AAH24342.1.
    BC145701 mRNA. Translation: AAI45702.1.
    CCDSiCCDS15363.1.
    RefSeqiNP_079953.2. NM_025677.3.
    UniGeneiMm.379120.

    Genome annotation databases

    GeneIDi66637.
    KEGGimmu:66637.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK017650 mRNA. Translation: BAB30855.1 .
    BC024342 mRNA. Translation: AAH24342.1 .
    BC145701 mRNA. Translation: AAI45702.1 .
    CCDSi CCDS15363.1.
    RefSeqi NP_079953.2. NM_025677.3.
    UniGenei Mm.379120.

    3D structure databases

    ProteinModelPortali Q8R3W5.
    SMRi Q8R3W5. Positions 33-154.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 211612. 1 interaction.

    Proteomic databases

    PaxDbi Q8R3W5.
    PRIDEi Q8R3W5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 66637.
    KEGGi mmu:66637.

    Organism-specific databases

    CTDi 116461.
    MGIi MGI:1913887. Tsen15.

    Phylogenomic databases

    eggNOGi NOG44101.
    HOGENOMi HOG000290172.
    HOVERGENi HBG058504.
    InParanoidi A6H601.
    KOi K15324.
    PhylomeDBi Q8R3W5.

    Miscellaneous databases

    NextBioi 322238.
    PROi Q8R3W5.
    SOURCEi Search...

    Gene expression databases

    Genevestigatori Q8R3W5.

    Family and domain databases

    InterProi IPR018593. tRNA-endonuc_su_Sen15.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    [Graphical view ]
    Pfami PF09631. Sen15. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53032. SSF53032. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryo.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N-3.
      Tissue: Brain and Mammary gland.

    Entry informationi

    Entry nameiSEN15_MOUSE
    AccessioniPrimary (citable) accession number: Q8R3W5
    Secondary accession number(s): A6H601, Q9CQW8, Q9CYI5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    Although only weakly related to the S.cerevisiae SEN15 protein, it probably displays the same function within the tRNA splicing endonuclease complex.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3