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Protein

tRNA-splicing endonuclease subunit Sen15

Gene

Tsen15

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processmRNA processing, tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease subunit Sen15
Alternative name(s):
tRNA-intron endonuclease Sen15
Gene namesi
Name:Tsen15
Synonyms:Sen15
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1913887. Tsen15.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001940241 – 168tRNA-splicing endonuclease subunit Sen15Add BLAST168

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7PhosphoserineBy similarity1
Modified residuei165PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8R3W5.
PaxDbiQ8R3W5.
PeptideAtlasiQ8R3W5.
PRIDEiQ8R3W5.

PTM databases

PhosphoSitePlusiQ8R3W5.

Interactioni

Subunit structurei

Homodimer. tRNA splicing endonuclease is a heterotetramer composed of TSEN2, TSEN15, TSEN34/LENG5 and TSEN54. tRNA splicing endonuclease complex also contains proteins of the pre-mRNA 3' end processing machinery such as CLP1, CPSF1, CPSF4 and CSTF2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi211612. 1 interactor.
STRINGi10090.ENSMUSP00000015124.

Structurei

3D structure databases

ProteinModelPortaliQ8R3W5.
SMRiQ8R3W5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SEN15 family.Curated

Phylogenomic databases

eggNOGiENOG410IYHQ. Eukaryota.
ENOG41120H2. LUCA.
HOGENOMiHOG000290172.
HOVERGENiHBG058504.
InParanoidiQ8R3W5.
KOiK15324.
PhylomeDBiQ8R3W5.

Family and domain databases

InterProiView protein in InterPro
IPR018593. tRNA-endonuc_su_Sen15.
IPR006677. tRNA_intron_Endonuc_cat-like.
PfamiView protein in Pfam
PF09631. Sen15. 1 hit.
SUPFAMiSSF53032. SSF53032. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8R3W5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEERSDSEPT PGCSGPGPAP VRDGGGAHTW APEDAWMGTH PKYLEMMELD
60 70 80 90 100
IGDATQVYIA FLVYLDLMES KSWHEVNCVG IPELQLICLL GTEIEGEGLQ
110 120 130 140 150
TVVPTPISAS LSHNRIREIL KASRKLQGDP ELPMSFTLAI VESDSTIVYY
160
KLTDGFMLPD PQNISLRR
Length:168
Mass (Da):18,530
Last modified:June 1, 2002 - v1
Checksum:i99D8E6E14C2FCAA0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5 – 8SDSE → TSSK in BAB30855 (PubMed:16141072).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017650 mRNA. Translation: BAB30855.1.
BC024342 mRNA. Translation: AAH24342.1.
BC145701 mRNA. Translation: AAI45702.1.
CCDSiCCDS15363.1.
RefSeqiNP_079953.2. NM_025677.3.
UniGeneiMm.379120.

Genome annotation databases

GeneIDi66637.
KEGGimmu:66637.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSEN15_MOUSE
AccessioniPrimary (citable) accession number: Q8R3W5
Secondary accession number(s): A6H601, Q9CQW8, Q9CYI5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: June 1, 2002
Last modified: May 10, 2017
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Although only weakly related to the S.cerevisiae SEN15 protein, it probably displays the same function within the tRNA splicing endonuclease complex.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families