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Q8R3Q0 (SARAF_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Store-operated calcium entry-associated regulatory factor

Short name=SARAF
Short name=SOCE-associated regulatory factor
Alternative name(s):
Transmembrane protein 66
Gene names
Name:Tmem66
Synonyms:Saraf
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Negative regulator of store-operated Ca2+ entry (SOCE) involved in protecting cells from Ca2+ overfilling. In response to cytosolic Ca2+ elevation after endoplasmic reticulum Ca2+ refilling, promotes a slow inactivation of STIM (STIM1 or STIM2)-dependent SOCE activity: possibly act by facilitating the deoligomerization of STIM to efficiently turn off ORAI when the endoplasmic reticulum lumen is filled with the appropriate Ca2+ levels, and thus preventing the overload of the cell with excessive Ca2+ ions By similarity.

Subunit structure

Interacts with STIM1 By similarity.

Subcellular location

Endoplasmic reticulum membrane; Single-pass type I membrane protein By similarity. Note: Translocates to the endoplasmic reticulum-plasma membrane (ER-PM) region in a STIM1-dependent manner following cytosolic Ca2+ elevation By similarity.

Domain

The cytoplasmic C-terminal region mediates interaction with STIM1, while the N-terminal lumenal region mediates regulation of SOCE activity By similarity.

Sequence similarities

Belongs to the SARAF family.

Sequence caution

The sequence AAH13497.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAH22616.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAH24888.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAB25254.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAC04255.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAC40531.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAE37240.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Chain32 – 334303Store-operated calcium entry-associated regulatory factor
PRO_0000045486

Regions

Topological domain32 – 168137Lumenal Potential
Transmembrane169 – 18921Helical; Potential
Topological domain190 – 334145Cytoplasmic Potential

Experimental info

Sequence conflict121L → V in BAC04255. Ref.2
Sequence conflict121L → V in BAC40531. Ref.2
Sequence conflict121L → V in AAH24888. Ref.3
Sequence conflict1171G → V in AAH22616. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q8R3Q0 [UniParc].

Last modified January 10, 2006. Version 2.
Checksum: EA12FE138052BCC4

FASTA33435,856
        10         20         30         40         50         60 
MAVAAVGRPR ALRCPLLLLL SLLLVAGPAL GWNDPDRILL RDVKALTLYS DRYTTSRRLD 

        70         80         90        100        110        120 
PIPQLKCVGG TAGCEAYTPR VIQCQNKGWD GYDVQWECKT DLDIAYKFGK TVVSCEGYES 

       130        140        150        160        170        180 
SEDQYVLRGS CGLEYNLDYT ELGLKKLKES GKHQGFSDYY HKLYSSDSCG FITIAVLFVL 

       190        200        210        220        230        240 
AFAVYKLFLS DGQGSPPPYS EHPPYSEHSQ RFASAAGAPP PGFKSEFTGP QNTGYGASSG 

       250        260        270        280        290        300 
FGSAFGGQGY GSSGPGFWSG LGAGGLLGYL FGSNRAATPF SDSWYHPAYP PSHSGAWNSR 

       310        320        330 
AYSPLGGGAG SYCASSNADS RTRTASGYGG TRRR 

« Hide

References

[1]"NEDO cDNA sequencing project."
Suzuki O., Sasaki N., Aotsuka S., Shoji T., Ichihara T., Shiohata N., Matsumoto K., Hirano M., Sano S., Nomura R., Yoshikawa Y., Matsumura Y., Moriya S., Chiba E., Momiyama H., Onogawa S., Kaeriyama S., Satoh N. expand/collapse author list , Matsunawa H., Takahashi E., Kataoka R., Kuga N., Kuroda A., Satoh I., Kamata K., Takami S., Terashima Y., Watanabe M., Sugiyama T., Irie R., Otsuki T., Sato H., Ota T., Wakamatsu A., Ishii S., Yamamoto J., Isono Y., Kawai-Hio Y., Saito K., Nishikawa T., Kimura K., Yamashita H., Matsuo K., Nakamura Y., Sekine M., Kikuchi H., Kanda K., Wagatsuma M., Murakawa K., Kanehori K., Takahashi-Fujii A., Oshima A., Sugiyama A., Kawakami B., Suzuki Y., Sugano S., Nagahari K., Masuho Y., Nagai K., Isogai T.
Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Egg, Pancreas, Placenta and Thymus.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Czech II.
Tissue: Mammary tumor and Salivary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK093942 mRNA. Translation: BAC04255.1. Different initiation.
AK007787 mRNA. Translation: BAB25254.1. Different initiation.
AK075744 mRNA. Translation: BAC35924.1.
AK088728 mRNA. Translation: BAC40531.1. Different initiation.
AK163218 mRNA. Translation: BAE37240.1. Different initiation.
BC013497 mRNA. Translation: AAH13497.1. Different initiation.
BC022616 mRNA. Translation: AAH22616.1. Different initiation.
BC024888 mRNA. Translation: AAH24888.1. Different initiation.
RefSeqNP_080708.3. NM_026432.3.
UniGeneMm.290353.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8R3Q0. 1 interaction.

PTM databases

PhosphoSiteQ8R3Q0.

Proteomic databases

PaxDbQ8R3Q0.
PRIDEQ8R3Q0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000033933; ENSMUSP00000033933; ENSMUSG00000031532.
GeneID67887.
KEGGmmu:67887.
UCSCuc012gci.1. mouse.

Organism-specific databases

CTD51669.
MGIMGI:1915137. Tmem66.

Phylogenomic databases

eggNOGNOG85111.
GeneTreeENSGT00390000013419.
HOGENOMHOG000154648.
HOVERGENHBG059843.
InParanoidQ8R3Q0.
OrthoDBEOG7QG462.
PhylomeDBQ8R3Q0.
TreeFamTF314811.

Gene expression databases

BgeeQ8R3Q0.
CleanExMM_TMEM66.
GenevestigatorQ8R3Q0.

Family and domain databases

InterProIPR009567. DUF1183_TMEM66.
[Graphical view]
PANTHERPTHR15929. PTHR15929. 1 hit.
PfamPF06682. DUF1183. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTMEM66. mouse.
NextBio325846.
PROQ8R3Q0.
SOURCESearch...

Entry information

Entry nameSARAF_MOUSE
AccessionPrimary (citable) accession number: Q8R3Q0
Secondary accession number(s): Q8C2F1 expand/collapse secondary AC list , Q8C6G7, Q8N9S4, Q8R233, Q9D8R1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: January 10, 2006
Last modified: April 16, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot