Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8R3Q0

- SARAF_MOUSE

UniProt

Q8R3Q0 - SARAF_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Store-operated calcium entry-associated regulatory factor

Gene

Saraf

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Negative regulator of store-operated Ca2+ entry (SOCE) involved in protecting cells from Ca2+ overfilling. In response to cytosolic Ca2+ elevation after endoplasmic reticulum Ca2+ refilling, promotes a slow inactivation of STIM (STIM1 or STIM2)-dependent SOCE activity: possibly act by facilitating the deoligomerization of STIM to efficiently turn off ORAI when the endoplasmic reticulum lumen is filled with the appropriate Ca2+ levels, and thus preventing the overload of the cell with excessive Ca2+ ions By similarity.By similarity

GO - Molecular functioni

  1. store-operated calcium channel activity Source: Ensembl

GO - Biological processi

  1. regulation of store-operated calcium entry Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Store-operated calcium entry-associated regulatory factor
Short name:
SARAF
Short name:
SOCE-associated regulatory factor
Alternative name(s):
Transmembrane protein 66
Gene namesi
Name:Saraf
Synonyms:Tmem66
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:1915137. Saraf.

Subcellular locationi

Endoplasmic reticulum membrane By similarity; Single-pass type I membrane protein By similarity
Note: Translocates to the endoplasmic reticulum-plasma membrane (ER-PM) region in a STIM1-dependent manner following cytosolic Ca2+ elevation.By similarity

GO - Cellular componenti

  1. integral component of endoplasmic reticulum membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence AnalysisAdd
BLAST
Chaini32 – 334303Store-operated calcium entry-associated regulatory factorPRO_0000045486Add
BLAST

Proteomic databases

MaxQBiQ8R3Q0.
PaxDbiQ8R3Q0.
PRIDEiQ8R3Q0.

PTM databases

PhosphoSiteiQ8R3Q0.

Expressioni

Gene expression databases

BgeeiQ8R3Q0.
CleanExiMM_TMEM66.
GenevestigatoriQ8R3Q0.

Interactioni

Subunit structurei

Interacts with STIM1.By similarity

Protein-protein interaction databases

IntActiQ8R3Q0. 1 interaction.

Structurei

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini32 – 168137LumenalSequence AnalysisAdd
BLAST
Topological domaini190 – 334145CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei169 – 18921HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domaini

The cytoplasmic C-terminal region mediates interaction with STIM1, while the N-terminal lumenal region mediates regulation of SOCE activity.By similarity

Sequence similaritiesi

Belongs to the SARAF family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG85111.
GeneTreeiENSGT00390000013419.
HOGENOMiHOG000154648.
HOVERGENiHBG059843.
InParanoidiQ8R3Q0.
OrthoDBiEOG7QG462.
PhylomeDBiQ8R3Q0.
TreeFamiTF314811.

Family and domain databases

InterProiIPR009567. DUF1183_TMEM66.
[Graphical view]
PANTHERiPTHR15929. PTHR15929. 1 hit.
PfamiPF06682. DUF1183. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R3Q0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVAAVGRPR ALRCPLLLLL SLLLVAGPAL GWNDPDRILL RDVKALTLYS
60 70 80 90 100
DRYTTSRRLD PIPQLKCVGG TAGCEAYTPR VIQCQNKGWD GYDVQWECKT
110 120 130 140 150
DLDIAYKFGK TVVSCEGYES SEDQYVLRGS CGLEYNLDYT ELGLKKLKES
160 170 180 190 200
GKHQGFSDYY HKLYSSDSCG FITIAVLFVL AFAVYKLFLS DGQGSPPPYS
210 220 230 240 250
EHPPYSEHSQ RFASAAGAPP PGFKSEFTGP QNTGYGASSG FGSAFGGQGY
260 270 280 290 300
GSSGPGFWSG LGAGGLLGYL FGSNRAATPF SDSWYHPAYP PSHSGAWNSR
310 320 330
AYSPLGGGAG SYCASSNADS RTRTASGYGG TRRR
Length:334
Mass (Da):35,856
Last modified:January 10, 2006 - v2
Checksum:iEA12FE138052BCC4
GO

Sequence cautioni

The sequence AAH13497.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAH22616.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAH24888.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAB25254.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAC04255.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAC40531.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAE37240.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121L → V in BAC04255. (PubMed:16141072)Curated
Sequence conflicti12 – 121L → V in BAC40531. (PubMed:16141072)Curated
Sequence conflicti12 – 121L → V in AAH24888. (PubMed:15489334)Curated
Sequence conflicti117 – 1171G → V in AAH22616. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK093942 mRNA. Translation: BAC04255.1. Different initiation.
AK007787 mRNA. Translation: BAB25254.1. Different initiation.
AK075744 mRNA. Translation: BAC35924.1.
AK088728 mRNA. Translation: BAC40531.1. Different initiation.
AK163218 mRNA. Translation: BAE37240.1. Different initiation.
BC013497 mRNA. Translation: AAH13497.1. Different initiation.
BC022616 mRNA. Translation: AAH22616.1. Different initiation.
BC024888 mRNA. Translation: AAH24888.1. Different initiation.
RefSeqiNP_080708.3. NM_026432.3.
UniGeneiMm.290353.

Genome annotation databases

EnsembliENSMUST00000033933; ENSMUSP00000033933; ENSMUSG00000031532.
GeneIDi67887.
KEGGimmu:67887.
UCSCiuc012gci.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK093942 mRNA. Translation: BAC04255.1 . Different initiation.
AK007787 mRNA. Translation: BAB25254.1 . Different initiation.
AK075744 mRNA. Translation: BAC35924.1 .
AK088728 mRNA. Translation: BAC40531.1 . Different initiation.
AK163218 mRNA. Translation: BAE37240.1 . Different initiation.
BC013497 mRNA. Translation: AAH13497.1 . Different initiation.
BC022616 mRNA. Translation: AAH22616.1 . Different initiation.
BC024888 mRNA. Translation: AAH24888.1 . Different initiation.
RefSeqi NP_080708.3. NM_026432.3.
UniGenei Mm.290353.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8R3Q0. 1 interaction.

PTM databases

PhosphoSitei Q8R3Q0.

Proteomic databases

MaxQBi Q8R3Q0.
PaxDbi Q8R3Q0.
PRIDEi Q8R3Q0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000033933 ; ENSMUSP00000033933 ; ENSMUSG00000031532 .
GeneIDi 67887.
KEGGi mmu:67887.
UCSCi uc012gci.1. mouse.

Organism-specific databases

CTDi 51669.
MGIi MGI:1915137. Saraf.

Phylogenomic databases

eggNOGi NOG85111.
GeneTreei ENSGT00390000013419.
HOGENOMi HOG000154648.
HOVERGENi HBG059843.
InParanoidi Q8R3Q0.
OrthoDBi EOG7QG462.
PhylomeDBi Q8R3Q0.
TreeFami TF314811.

Miscellaneous databases

ChiTaRSi TMEM66. mouse.
NextBioi 325846.
PROi Q8R3Q0.
SOURCEi Search...

Gene expression databases

Bgeei Q8R3Q0.
CleanExi MM_TMEM66.
Genevestigatori Q8R3Q0.

Family and domain databases

InterProi IPR009567. DUF1183_TMEM66.
[Graphical view ]
PANTHERi PTHR15929. PTHR15929. 1 hit.
Pfami PF06682. DUF1183. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Egg, Pancreas, Placenta and Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary tumor and Salivary gland.

Entry informationi

Entry nameiSARAF_MOUSE
AccessioniPrimary (citable) accession number: Q8R3Q0
Secondary accession number(s): Q8C2F1
, Q8C6G7, Q8N9S4, Q8R233, Q9D8R1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: January 10, 2006
Last modified: October 29, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3