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Protein

Store-operated calcium entry-associated regulatory factor

Gene

Saraf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Negative regulator of store-operated Ca2+ entry (SOCE) involved in protecting cells from Ca2+ overfilling. In response to cytosolic Ca2+ elevation after endoplasmic reticulum Ca2+ refilling, promotes a slow inactivation of STIM (STIM1 or STIM2)-dependent SOCE activity: possibly act by facilitating the deoligomerization of STIM to efficiently turn off ORAI when the endoplasmic reticulum lumen is filled with the appropriate Ca2+ levels, and thus preventing the overload of the cell with excessive Ca2+ ions (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Store-operated calcium entry-associated regulatory factor
Short name:
SARAF
Short name:
SOCE-associated regulatory factor
Alternative name(s):
Transmembrane protein 66
Gene namesi
Name:Saraf
Synonyms:Tmem66
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1915137. Saraf.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini32 – 168LumenalSequence analysisAdd BLAST137
Transmembranei169 – 189HelicalSequence analysisAdd BLAST21
Topological domaini190 – 334CytoplasmicSequence analysisAdd BLAST145

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_000004548632 – 334Store-operated calcium entry-associated regulatory factorAdd BLAST303

Proteomic databases

EPDiQ8R3Q0.
MaxQBiQ8R3Q0.
PaxDbiQ8R3Q0.
PeptideAtlasiQ8R3Q0.
PRIDEiQ8R3Q0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031532.
CleanExiMM_TMEM66.

Interactioni

Subunit structurei

Interacts with STIM1.By similarity

Protein-protein interaction databases

IntActiQ8R3Q0. 1 interactor.
STRINGi10090.ENSMUSP00000033933.

Structurei

3D structure databases

ProteinModelPortaliQ8R3Q0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The cytoplasmic C-terminal region mediates interaction with STIM1, while the N-terminal lumenal region mediates regulation of SOCE activity.By similarity

Sequence similaritiesi

Belongs to the SARAF family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IV9Z. Eukaryota.
ENOG4111IKY. LUCA.
HOGENOMiHOG000154648.
HOVERGENiHBG059843.
InParanoidiQ8R3Q0.
PhylomeDBiQ8R3Q0.
TreeFamiTF314811.

Family and domain databases

InterProiIPR009567. SARAF.
[Graphical view]
PANTHERiPTHR15929. PTHR15929. 1 hit.
PfamiPF06682. SARAF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R3Q0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVAAVGRPR ALRCPLLLLL SLLLVAGPAL GWNDPDRILL RDVKALTLYS
60 70 80 90 100
DRYTTSRRLD PIPQLKCVGG TAGCEAYTPR VIQCQNKGWD GYDVQWECKT
110 120 130 140 150
DLDIAYKFGK TVVSCEGYES SEDQYVLRGS CGLEYNLDYT ELGLKKLKES
160 170 180 190 200
GKHQGFSDYY HKLYSSDSCG FITIAVLFVL AFAVYKLFLS DGQGSPPPYS
210 220 230 240 250
EHPPYSEHSQ RFASAAGAPP PGFKSEFTGP QNTGYGASSG FGSAFGGQGY
260 270 280 290 300
GSSGPGFWSG LGAGGLLGYL FGSNRAATPF SDSWYHPAYP PSHSGAWNSR
310 320 330
AYSPLGGGAG SYCASSNADS RTRTASGYGG TRRR
Length:334
Mass (Da):35,856
Last modified:January 10, 2006 - v2
Checksum:iEA12FE138052BCC4
GO

Sequence cautioni

The sequence AAH13497 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH22616 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH24888 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB25254 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC04255 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC40531 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE37240 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12L → V in BAC04255 (PubMed:16141072).Curated1
Sequence conflicti12L → V in BAC40531 (PubMed:16141072).Curated1
Sequence conflicti12L → V in AAH24888 (PubMed:15489334).Curated1
Sequence conflicti117G → V in AAH22616 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK093942 mRNA. Translation: BAC04255.1. Different initiation.
AK007787 mRNA. Translation: BAB25254.1. Different initiation.
AK075744 mRNA. Translation: BAC35924.1.
AK088728 mRNA. Translation: BAC40531.1. Different initiation.
AK163218 mRNA. Translation: BAE37240.1. Different initiation.
BC013497 mRNA. Translation: AAH13497.1. Different initiation.
BC022616 mRNA. Translation: AAH22616.1. Different initiation.
BC024888 mRNA. Translation: AAH24888.1. Different initiation.
RefSeqiNP_080708.3. NM_026432.3.
UniGeneiMm.290353.

Genome annotation databases

GeneIDi67887.
KEGGimmu:67887.
UCSCiuc012gci.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK093942 mRNA. Translation: BAC04255.1. Different initiation.
AK007787 mRNA. Translation: BAB25254.1. Different initiation.
AK075744 mRNA. Translation: BAC35924.1.
AK088728 mRNA. Translation: BAC40531.1. Different initiation.
AK163218 mRNA. Translation: BAE37240.1. Different initiation.
BC013497 mRNA. Translation: AAH13497.1. Different initiation.
BC022616 mRNA. Translation: AAH22616.1. Different initiation.
BC024888 mRNA. Translation: AAH24888.1. Different initiation.
RefSeqiNP_080708.3. NM_026432.3.
UniGeneiMm.290353.

3D structure databases

ProteinModelPortaliQ8R3Q0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8R3Q0. 1 interactor.
STRINGi10090.ENSMUSP00000033933.

Proteomic databases

EPDiQ8R3Q0.
MaxQBiQ8R3Q0.
PaxDbiQ8R3Q0.
PeptideAtlasiQ8R3Q0.
PRIDEiQ8R3Q0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi67887.
KEGGimmu:67887.
UCSCiuc012gci.1. mouse.

Organism-specific databases

CTDi51669.
MGIiMGI:1915137. Saraf.

Phylogenomic databases

eggNOGiENOG410IV9Z. Eukaryota.
ENOG4111IKY. LUCA.
HOGENOMiHOG000154648.
HOVERGENiHBG059843.
InParanoidiQ8R3Q0.
PhylomeDBiQ8R3Q0.
TreeFamiTF314811.

Miscellaneous databases

ChiTaRSiTmem66. mouse.
PROiQ8R3Q0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031532.
CleanExiMM_TMEM66.

Family and domain databases

InterProiIPR009567. SARAF.
[Graphical view]
PANTHERiPTHR15929. PTHR15929. 1 hit.
PfamiPF06682. SARAF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSARAF_MOUSE
AccessioniPrimary (citable) accession number: Q8R3Q0
Secondary accession number(s): Q8C2F1
, Q8C6G7, Q8N9S4, Q8R233, Q9D8R1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: January 10, 2006
Last modified: November 2, 2016
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.