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Q8R3Q0

- SARAF_MOUSE

UniProt

Q8R3Q0 - SARAF_MOUSE

Protein

Store-operated calcium entry-associated regulatory factor

Gene

Saraf

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 2 (10 Jan 2006)
      Previous versions | rss
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    Functioni

    Negative regulator of store-operated Ca2+ entry (SOCE) involved in protecting cells from Ca2+ overfilling. In response to cytosolic Ca2+ elevation after endoplasmic reticulum Ca2+ refilling, promotes a slow inactivation of STIM (STIM1 or STIM2)-dependent SOCE activity: possibly act by facilitating the deoligomerization of STIM to efficiently turn off ORAI when the endoplasmic reticulum lumen is filled with the appropriate Ca2+ levels, and thus preventing the overload of the cell with excessive Ca2+ ions By similarity.By similarity

    GO - Molecular functioni

    1. store-operated calcium channel activity Source: UniProtKB

    GO - Biological processi

    1. calcium ion transmembrane transport Source: GOC
    2. regulation of store-operated calcium entry Source: UniProtKB

    Keywords - Biological processi

    Calcium transport, Ion transport, Transport

    Keywords - Ligandi

    Calcium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Store-operated calcium entry-associated regulatory factor
    Short name:
    SARAF
    Short name:
    SOCE-associated regulatory factor
    Alternative name(s):
    Transmembrane protein 66
    Gene namesi
    Name:Saraf
    Synonyms:Tmem66
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:1915137. Tmem66.

    Subcellular locationi

    Endoplasmic reticulum membrane By similarity; Single-pass type I membrane protein By similarity
    Note: Translocates to the endoplasmic reticulum-plasma membrane (ER-PM) region in a STIM1-dependent manner following cytosolic Ca2+ elevation.By similarity

    GO - Cellular componenti

    1. integral component of endoplasmic reticulum membrane Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Sequence AnalysisAdd
    BLAST
    Chaini32 – 334303Store-operated calcium entry-associated regulatory factorPRO_0000045486Add
    BLAST

    Proteomic databases

    PaxDbiQ8R3Q0.
    PRIDEiQ8R3Q0.

    PTM databases

    PhosphoSiteiQ8R3Q0.

    Expressioni

    Gene expression databases

    BgeeiQ8R3Q0.
    CleanExiMM_TMEM66.
    GenevestigatoriQ8R3Q0.

    Interactioni

    Subunit structurei

    Interacts with STIM1.By similarity

    Protein-protein interaction databases

    IntActiQ8R3Q0. 1 interaction.

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini32 – 168137LumenalSequence AnalysisAdd
    BLAST
    Topological domaini190 – 334145CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei169 – 18921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domaini

    The cytoplasmic C-terminal region mediates interaction with STIM1, while the N-terminal lumenal region mediates regulation of SOCE activity.By similarity

    Sequence similaritiesi

    Belongs to the SARAF family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG85111.
    GeneTreeiENSGT00390000013419.
    HOGENOMiHOG000154648.
    HOVERGENiHBG059843.
    InParanoidiQ8R3Q0.
    OrthoDBiEOG7QG462.
    PhylomeDBiQ8R3Q0.
    TreeFamiTF314811.

    Family and domain databases

    InterProiIPR009567. DUF1183_TMEM66.
    [Graphical view]
    PANTHERiPTHR15929. PTHR15929. 1 hit.
    PfamiPF06682. DUF1183. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8R3Q0-1 [UniParc]FASTAAdd to Basket

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    MAVAAVGRPR ALRCPLLLLL SLLLVAGPAL GWNDPDRILL RDVKALTLYS    50
    DRYTTSRRLD PIPQLKCVGG TAGCEAYTPR VIQCQNKGWD GYDVQWECKT 100
    DLDIAYKFGK TVVSCEGYES SEDQYVLRGS CGLEYNLDYT ELGLKKLKES 150
    GKHQGFSDYY HKLYSSDSCG FITIAVLFVL AFAVYKLFLS DGQGSPPPYS 200
    EHPPYSEHSQ RFASAAGAPP PGFKSEFTGP QNTGYGASSG FGSAFGGQGY 250
    GSSGPGFWSG LGAGGLLGYL FGSNRAATPF SDSWYHPAYP PSHSGAWNSR 300
    AYSPLGGGAG SYCASSNADS RTRTASGYGG TRRR 334
    Length:334
    Mass (Da):35,856
    Last modified:January 10, 2006 - v2
    Checksum:iEA12FE138052BCC4
    GO

    Sequence cautioni

    The sequence AAH13497.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAH22616.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAH24888.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAB25254.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAC04255.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAC40531.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAE37240.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti12 – 121L → V in BAC04255. (PubMed:16141072)Curated
    Sequence conflicti12 – 121L → V in BAC40531. (PubMed:16141072)Curated
    Sequence conflicti12 – 121L → V in AAH24888. (PubMed:15489334)Curated
    Sequence conflicti117 – 1171G → V in AAH22616. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK093942 mRNA. Translation: BAC04255.1. Different initiation.
    AK007787 mRNA. Translation: BAB25254.1. Different initiation.
    AK075744 mRNA. Translation: BAC35924.1.
    AK088728 mRNA. Translation: BAC40531.1. Different initiation.
    AK163218 mRNA. Translation: BAE37240.1. Different initiation.
    BC013497 mRNA. Translation: AAH13497.1. Different initiation.
    BC022616 mRNA. Translation: AAH22616.1. Different initiation.
    BC024888 mRNA. Translation: AAH24888.1. Different initiation.
    RefSeqiNP_080708.3. NM_026432.3.
    UniGeneiMm.290353.

    Genome annotation databases

    EnsembliENSMUST00000033933; ENSMUSP00000033933; ENSMUSG00000031532.
    GeneIDi67887.
    KEGGimmu:67887.
    UCSCiuc012gci.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK093942 mRNA. Translation: BAC04255.1 . Different initiation.
    AK007787 mRNA. Translation: BAB25254.1 . Different initiation.
    AK075744 mRNA. Translation: BAC35924.1 .
    AK088728 mRNA. Translation: BAC40531.1 . Different initiation.
    AK163218 mRNA. Translation: BAE37240.1 . Different initiation.
    BC013497 mRNA. Translation: AAH13497.1 . Different initiation.
    BC022616 mRNA. Translation: AAH22616.1 . Different initiation.
    BC024888 mRNA. Translation: AAH24888.1 . Different initiation.
    RefSeqi NP_080708.3. NM_026432.3.
    UniGenei Mm.290353.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8R3Q0. 1 interaction.

    PTM databases

    PhosphoSitei Q8R3Q0.

    Proteomic databases

    PaxDbi Q8R3Q0.
    PRIDEi Q8R3Q0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033933 ; ENSMUSP00000033933 ; ENSMUSG00000031532 .
    GeneIDi 67887.
    KEGGi mmu:67887.
    UCSCi uc012gci.1. mouse.

    Organism-specific databases

    CTDi 67887.
    MGIi MGI:1915137. Tmem66.

    Phylogenomic databases

    eggNOGi NOG85111.
    GeneTreei ENSGT00390000013419.
    HOGENOMi HOG000154648.
    HOVERGENi HBG059843.
    InParanoidi Q8R3Q0.
    OrthoDBi EOG7QG462.
    PhylomeDBi Q8R3Q0.
    TreeFami TF314811.

    Miscellaneous databases

    ChiTaRSi TMEM66. mouse.
    NextBioi 325846.
    PROi Q8R3Q0.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8R3Q0.
    CleanExi MM_TMEM66.
    Genevestigatori Q8R3Q0.

    Family and domain databases

    InterProi IPR009567. DUF1183_TMEM66.
    [Graphical view ]
    PANTHERi PTHR15929. PTHR15929. 1 hit.
    Pfami PF06682. DUF1183. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Egg, Pancreas, Placenta and Thymus.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
      Tissue: Mammary tumor and Salivary gland.

    Entry informationi

    Entry nameiSARAF_MOUSE
    AccessioniPrimary (citable) accession number: Q8R3Q0
    Secondary accession number(s): Q8C2F1
    , Q8C6G7, Q8N9S4, Q8R233, Q9D8R1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: January 10, 2006
    Last modified: October 1, 2014
    This is version 85 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3