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Protein

SMC5-SMC6 complex localization factor protein 1

Gene

Slf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in the DNA damage response (DDR) pathway by regulating postreplication repair of UV-damaged DNA and genomic stability maintenance. The SLF1-SLF2 complex acts to link RAD18 with the SMC5-SMC6 complex at replication-coupled interstrand cross-links (ICL) and DNA double-strand breaks (DSBs) sites on chromatin during DNA repair in response to stalled replication forks. Promotes the recruitment of SLF2 and the SMC5-SMC6 complex to DNA lesions.By similarity

GO - Molecular functioni

  • protein-containing complex binding Source: MGI
  • ubiquitin protein ligase binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processDNA damage, DNA repair

Names & Taxonomyi

Protein namesi
Recommended name:
SMC5-SMC6 complex localization factor protein 1Imported
Alternative name(s):
Ankyrin repeat domain-containing protein 32
BRCT domain-containing protein 1
Protein BRCTx1 Publication
Gene namesi
Name:Slf1Imported
Synonyms:Ankrd32, Brctd1, Brctx1 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi

Organism-specific databases

MGIiMGI:2145448 Slf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice develop normally, display no pathological abnormalities and are fertile (PubMed:15632077).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002439071 – 1054SMC5-SMC6 complex localization factor protein 1Add BLAST1054

Proteomic databases

EPDiQ8R3P9
PaxDbiQ8R3P9
PRIDEiQ8R3P9

PTM databases

iPTMnetiQ8R3P9
PhosphoSitePlusiQ8R3P9

Expressioni

Tissue specificityi

Widely expressed (PubMed:15632077). Expressed in testis (PubMed:15632077). Expressed in spermatocytes (PubMed:15632077).1 Publication

Developmental stagei

Expressed in the developing embryo (PubMed:15632077).1 Publication

Gene expression databases

BgeeiENSMUSG00000021597
CleanExiMM_ANKRD32
ExpressionAtlasiQ8R3P9 baseline and differential
GenevisibleiQ8R3P9 MM

Interactioni

Subunit structurei

Interacts (via BRCT domains) with RAD18 (via C-terminus and phosphorylated form); this interaction is required for efficient repair of UV-induced DNA damage (PubMed:15632077, PubMed:22036607). Interacts (via N-terminus) with SLF2; this interaction links RAD18 to the SMC5-SMC6 complex. Interacts (via BRCT domains) with RAD18; this interaction occurs in a SLF2-independent manner. Interacts with SMC6.By similarity2 Publications

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi222830, 4 interactors
STRINGi10090.ENSMUSP00000118312

Structurei

3D structure databases

ProteinModelPortaliQ8R3P9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 80BRCT 1Add BLAST79
Domaini121 – 199BRCT 2Add BLAST79
Repeati802 – 832ANK 1Add BLAST31
Repeati836 – 865ANK 2Add BLAST30
Repeati870 – 900ANK 3Add BLAST31

Domaini

BRCT domains are necessary for its targeting to ionizing radiation-induced nuclear foci (PubMed:22036607).1 Publication

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0504 Eukaryota
KOG1929 Eukaryota
ENOG410XPFH LUCA
GeneTreeiENSGT00760000119090
HOGENOMiHOG000200317
HOVERGENiHBG103143
InParanoidiQ8R3P9
OMAiPFYPIQY
OrthoDBiEOG091G0JQ3
PhylomeDBiQ8R3P9
TreeFamiTF329705

Family and domain databases

CDDicd00204 ANK, 1 hit
cd00027 BRCT, 1 hit
Gene3Di1.25.40.20, 1 hit
3.40.50.10190, 2 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF16770 RTT107_BRCT_5, 1 hit
SMARTiView protein in SMART
SM00248 ANK, 3 hits
SM00292 BRCT, 2 hits
SUPFAMiSSF48403 SSF48403, 1 hit
SSF52113 SSF52113, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 3 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R3P9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDSATKHII QMTGFKMEEK EALVKLLLKL DCTFIKSEKY KNCTHLIAER
60 70 80 90 100
LCKSEKFLAA CAAGKWVLTK DYIIHSAKSG RWLDETTYEW GYKIEKDSHY
110 120 130 140 150
SPQMQSAPKR WREELKRTGA PGAFHRWKVV LLVRADKRSD SLVRVLEAGK
160 170 180 190 200
ANVILPKNSP SGITHVIASN ARISAEREQE NFKAPFYPIQ YLGDFLLEKE
210 220 230 240 250
IQNDEHSQIS PAWTKYNNQE KGNDVGFPEM KGAGENMYRT QNKMENHNKN
260 270 280 290 300
VSDRFVLSEH HKKKFKDFRK DIRSVKKRNT LRRHGLENQK ETKKKDKNIQ
310 320 330 340 350
RSYILRKKNK KEGYCKTDDA HDTIRSMLKK RGHYREQKEM KNPLLTDGTK
360 370 380 390 400
ESKTKDVKTN MNLIEIKNAL KKQIYKDIYR AQAVRYNCIR VDKQPVYNVE
410 420 430 440 450
VKNSEFPRGI LNLIENLIEG QFFKEAIEEL SSLQAHYIPP VCLLHAILEN
460 470 480 490 500
VLQDKIDTFS GRYFHILSAL LHLHPPWKSP AMLKYYLELF QCPTCMKGAW
510 520 530 540 550
DFTEVLIRSC LFNEDFCHQI SENISTKVVN LTLLKFFFNL LEGEVRHLSQ
560 570 580 590 600
KLCDWSDSQS LKVTEKAILH EIFWSGSETS GLLTKPVNML LEWTIYSHKE
610 620 630 640 650
KCKSNDVFKH ELSYLLTGIL GAAVDYWIFL GIQMGRNVIR HMSDDLGSYI
660 670 680 690 700
SLSCDDFSSK ELEIFICSFS SSWLQMFVAE AIFKKLCLQG PTSTCTEPLS
710 720 730 740 750
LQKIIDSYLP ILGKMDIHGA GKMQSPKKLC QRPCLESQRA LLMLNGAKRK
760 770 780 790 800
QAEGRPELLE LNRAKCSSSL KKLKKKSEEL SCSKENCPSL VTKMNFHKTN
810 820 830 840 850
LKGETALHRV CIKNQVEKLI ILLSLPGIDI NVKDNAGWTP LHEACNYGNT
860 870 880 890 900
ECVQEILQRC PEVDLLTQVD GVTPLHDALS NGHVEIGKLL LQRGGPELLQ
910 920 930 940 950
QRNSKGELPL DYVLSPKDKE ELFAITNIDD TVDNFHAKTQ KHFYHQQLEF
960 970 980 990 1000
GSFLLSRMLI NFCSIFDLSS EFILAFKGLG HLNELLMACN SDTEASNAHT
1010 1020 1030 1040 1050
DWLLDVYARN IKTLKKLPSV LKELPENLNV CPGVHTEALL VTLKMMCQSI

TELS
Length:1,054
Mass (Da):121,004
Last modified:July 1, 2008 - v3
Checksum:i248B6D44E221D6CF
GO
Isoform 2 (identifier: Q8R3P9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     242-250: NKMENHNKN → VKFCELEIL
     251-1054: Missing.

Note: No experimental confirmation available.
Show »
Length:250
Mass (Da):28,777
Checksum:iEC437E521752D0D2
GO

Sequence cautioni

The sequence AAH09101 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH24900 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH83095 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti711I → V in AAH09101 (PubMed:15489334).Curated1
Sequence conflicti711I → V in AAH24900 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_034415242 – 250NKMENHNKN → VKFCELEIL in isoform 2. 1 Publication9
Alternative sequenceiVSP_034416251 – 1054Missing in isoform 2. 1 PublicationAdd BLAST804

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC122418 Genomic DNA No translation available.
AC161266 Genomic DNA No translation available.
BC009101 mRNA Translation: AAH09101.1 Different initiation.
BC024900 mRNA Translation: AAH24900.1 Different initiation.
BC083095 mRNA Translation: AAH83095.1 Different initiation.
BC100362 mRNA Translation: AAI00363.1
AK047775 mRNA Translation: BAC33152.1
AK050168 mRNA Translation: BAC34106.1
CCDSiCCDS26657.2 [Q8R3P9-1]
RefSeqiNP_598832.3, NM_134071.3 [Q8R3P9-1]
XP_017170840.1, XM_017315351.1 [Q8R3P9-1]
UniGeneiMm.209730

Genome annotation databases

EnsembliENSMUST00000151524; ENSMUSP00000118312; ENSMUSG00000021597 [Q8R3P9-1]
GeneIDi105377
KEGGimmu:105377
UCSCiuc007rgs.2 mouse [Q8R3P9-1]
uc007rgv.2 mouse [Q8R3P9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Entry informationi

Entry nameiSLF1_MOUSE
AccessioniPrimary (citable) accession number: Q8R3P9
Secondary accession number(s): Q497V2
, Q5XK30, Q8BHY5, Q8BQM6, Q921Y9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: July 1, 2008
Last modified: May 23, 2018
This is version 119 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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