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Protein

SMC5-SMC6 complex localization factor protein 1

Gene

Slf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the DNA damage response (DDR) pathway by regulating postreplication repair of UV-damaged DNA and genomic stability maintenance. The SLF1-SLF2 complex acts to link RAD18 with the SMC5-SMC6 complex at replication-coupled interstrand cross-links (ICL) and DNA double-strand breaks (DSBs) sites on chromatin during DNA repair in response to stalled replication forks. Promotes the recruitment of SLF2 and the SMC5-SMC6 complex to DNA lesions.By similarity

GO - Molecular functioni

  • protein complex binding Source: MGI
  • ubiquitin protein ligase binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Names & Taxonomyi

Protein namesi
Recommended name:
SMC5-SMC6 complex localization factor protein 1Imported
Alternative name(s):
Ankyrin repeat domain-containing protein 32
BRCT domain-containing protein 1
Protein BRCTx1 Publication
Gene namesi
Name:Slf1Imported
Synonyms:Ankrd32, Brctd1, Brctx1 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:2145448. Slf1.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: MGI
  • cytoplasm Source: UniProtKB
  • nuclear inclusion body Source: UniProtKB
  • nucleosome Source: UniProtKB
  • nucleus Source: UniProtKB
  • site of double-strand break Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice develop normally, display no pathological abnormalities and are fertile (PubMed:15632077).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002439071 – 1054SMC5-SMC6 complex localization factor protein 1Add BLAST1054

Proteomic databases

EPDiQ8R3P9.
PaxDbiQ8R3P9.
PRIDEiQ8R3P9.

PTM databases

iPTMnetiQ8R3P9.
PhosphoSitePlusiQ8R3P9.

Expressioni

Tissue specificityi

Widely expressed (PubMed:15632077). Expressed in testis (PubMed:15632077). Expressed in spermatocytes (PubMed:15632077).1 Publication

Developmental stagei

Expressed in the developing embryo (PubMed:15632077).1 Publication

Gene expression databases

BgeeiENSMUSG00000021597.
CleanExiMM_ANKRD32.
ExpressionAtlasiQ8R3P9. baseline and differential.
GenevisibleiQ8R3P9. MM.

Interactioni

Subunit structurei

Interacts (via BRCT domains) with RAD18 (via C-terminus and phosphorylated form); this interaction is required for efficient repair of UV-induced DNA damage (PubMed:15632077, PubMed:22036607). Interacts (via N-terminus) with SLF2; this interaction links RAD18 to the SMC5-SMC6 complex. Interacts (via BRCT domains) with RAD18; this interaction occurs in a SLF2-independent manner. Interacts with SMC6.By similarity2 Publications

GO - Molecular functioni

  • protein complex binding Source: MGI
  • ubiquitin protein ligase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi222830. 4 interactors.
STRINGi10090.ENSMUSP00000118312.

Structurei

3D structure databases

ProteinModelPortaliQ8R3P9.
SMRiQ8R3P9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 80BRCT 1Add BLAST79
Domaini121 – 199BRCT 2Add BLAST79
Repeati802 – 832ANK 1Add BLAST31
Repeati836 – 865ANK 2Add BLAST30
Repeati870 – 900ANK 3Add BLAST31

Domaini

BRCT domains are necessary for its targeting to ionizing radiation-induced nuclear foci (PubMed:22036607).1 Publication

Sequence similaritiesi

Contains 3 ANK repeats.PROSITE-ProRule annotation
Contains 2 BRCT domains.Curated

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
KOG1929. Eukaryota.
ENOG410XPFH. LUCA.
GeneTreeiENSGT00760000119090.
HOGENOMiHOG000200317.
HOVERGENiHBG103143.
InParanoidiQ8R3P9.
OMAiYLELFQC.
OrthoDBiEOG091G0JQ3.
PhylomeDBiQ8R3P9.
TreeFamiTF329705.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
Gene3Di1.25.40.20. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001357. BRCT_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF16770. RTT107_BRCT_5. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
SM00292. BRCT. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R3P9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDSATKHII QMTGFKMEEK EALVKLLLKL DCTFIKSEKY KNCTHLIAER
60 70 80 90 100
LCKSEKFLAA CAAGKWVLTK DYIIHSAKSG RWLDETTYEW GYKIEKDSHY
110 120 130 140 150
SPQMQSAPKR WREELKRTGA PGAFHRWKVV LLVRADKRSD SLVRVLEAGK
160 170 180 190 200
ANVILPKNSP SGITHVIASN ARISAEREQE NFKAPFYPIQ YLGDFLLEKE
210 220 230 240 250
IQNDEHSQIS PAWTKYNNQE KGNDVGFPEM KGAGENMYRT QNKMENHNKN
260 270 280 290 300
VSDRFVLSEH HKKKFKDFRK DIRSVKKRNT LRRHGLENQK ETKKKDKNIQ
310 320 330 340 350
RSYILRKKNK KEGYCKTDDA HDTIRSMLKK RGHYREQKEM KNPLLTDGTK
360 370 380 390 400
ESKTKDVKTN MNLIEIKNAL KKQIYKDIYR AQAVRYNCIR VDKQPVYNVE
410 420 430 440 450
VKNSEFPRGI LNLIENLIEG QFFKEAIEEL SSLQAHYIPP VCLLHAILEN
460 470 480 490 500
VLQDKIDTFS GRYFHILSAL LHLHPPWKSP AMLKYYLELF QCPTCMKGAW
510 520 530 540 550
DFTEVLIRSC LFNEDFCHQI SENISTKVVN LTLLKFFFNL LEGEVRHLSQ
560 570 580 590 600
KLCDWSDSQS LKVTEKAILH EIFWSGSETS GLLTKPVNML LEWTIYSHKE
610 620 630 640 650
KCKSNDVFKH ELSYLLTGIL GAAVDYWIFL GIQMGRNVIR HMSDDLGSYI
660 670 680 690 700
SLSCDDFSSK ELEIFICSFS SSWLQMFVAE AIFKKLCLQG PTSTCTEPLS
710 720 730 740 750
LQKIIDSYLP ILGKMDIHGA GKMQSPKKLC QRPCLESQRA LLMLNGAKRK
760 770 780 790 800
QAEGRPELLE LNRAKCSSSL KKLKKKSEEL SCSKENCPSL VTKMNFHKTN
810 820 830 840 850
LKGETALHRV CIKNQVEKLI ILLSLPGIDI NVKDNAGWTP LHEACNYGNT
860 870 880 890 900
ECVQEILQRC PEVDLLTQVD GVTPLHDALS NGHVEIGKLL LQRGGPELLQ
910 920 930 940 950
QRNSKGELPL DYVLSPKDKE ELFAITNIDD TVDNFHAKTQ KHFYHQQLEF
960 970 980 990 1000
GSFLLSRMLI NFCSIFDLSS EFILAFKGLG HLNELLMACN SDTEASNAHT
1010 1020 1030 1040 1050
DWLLDVYARN IKTLKKLPSV LKELPENLNV CPGVHTEALL VTLKMMCQSI

TELS
Length:1,054
Mass (Da):121,004
Last modified:July 1, 2008 - v3
Checksum:i248B6D44E221D6CF
GO
Isoform 2 (identifier: Q8R3P9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     242-250: NKMENHNKN → VKFCELEIL
     251-1054: Missing.

Note: No experimental confirmation available.
Show »
Length:250
Mass (Da):28,777
Checksum:iEC437E521752D0D2
GO

Sequence cautioni

The sequence AAH09101 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH24900 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH83095 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti711I → V in AAH09101 (PubMed:15489334).Curated1
Sequence conflicti711I → V in AAH24900 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_034415242 – 250NKMENHNKN → VKFCELEIL in isoform 2. 1 Publication9
Alternative sequenceiVSP_034416251 – 1054Missing in isoform 2. 1 PublicationAdd BLAST804

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC122418 Genomic DNA. No translation available.
AC161266 Genomic DNA. No translation available.
BC009101 mRNA. Translation: AAH09101.1. Different initiation.
BC024900 mRNA. Translation: AAH24900.1. Different initiation.
BC083095 mRNA. Translation: AAH83095.1. Different initiation.
BC100362 mRNA. Translation: AAI00363.1.
AK047775 mRNA. Translation: BAC33152.1.
AK050168 mRNA. Translation: BAC34106.1.
CCDSiCCDS26657.2. [Q8R3P9-1]
RefSeqiNP_598832.3. NM_134071.3. [Q8R3P9-1]
XP_017170840.1. XM_017315351.1. [Q8R3P9-1]
UniGeneiMm.209730.

Genome annotation databases

EnsembliENSMUST00000151524; ENSMUSP00000118312; ENSMUSG00000021597. [Q8R3P9-1]
GeneIDi105377.
KEGGimmu:105377.
UCSCiuc007rgs.2. mouse. [Q8R3P9-1]
uc007rgv.2. mouse. [Q8R3P9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC122418 Genomic DNA. No translation available.
AC161266 Genomic DNA. No translation available.
BC009101 mRNA. Translation: AAH09101.1. Different initiation.
BC024900 mRNA. Translation: AAH24900.1. Different initiation.
BC083095 mRNA. Translation: AAH83095.1. Different initiation.
BC100362 mRNA. Translation: AAI00363.1.
AK047775 mRNA. Translation: BAC33152.1.
AK050168 mRNA. Translation: BAC34106.1.
CCDSiCCDS26657.2. [Q8R3P9-1]
RefSeqiNP_598832.3. NM_134071.3. [Q8R3P9-1]
XP_017170840.1. XM_017315351.1. [Q8R3P9-1]
UniGeneiMm.209730.

3D structure databases

ProteinModelPortaliQ8R3P9.
SMRiQ8R3P9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi222830. 4 interactors.
STRINGi10090.ENSMUSP00000118312.

PTM databases

iPTMnetiQ8R3P9.
PhosphoSitePlusiQ8R3P9.

Proteomic databases

EPDiQ8R3P9.
PaxDbiQ8R3P9.
PRIDEiQ8R3P9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000151524; ENSMUSP00000118312; ENSMUSG00000021597. [Q8R3P9-1]
GeneIDi105377.
KEGGimmu:105377.
UCSCiuc007rgs.2. mouse. [Q8R3P9-1]
uc007rgv.2. mouse. [Q8R3P9-2]

Organism-specific databases

CTDi84250.
MGIiMGI:2145448. Slf1.

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
KOG1929. Eukaryota.
ENOG410XPFH. LUCA.
GeneTreeiENSGT00760000119090.
HOGENOMiHOG000200317.
HOVERGENiHBG103143.
InParanoidiQ8R3P9.
OMAiYLELFQC.
OrthoDBiEOG091G0JQ3.
PhylomeDBiQ8R3P9.
TreeFamiTF329705.

Miscellaneous databases

PROiQ8R3P9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021597.
CleanExiMM_ANKRD32.
ExpressionAtlasiQ8R3P9. baseline and differential.
GenevisibleiQ8R3P9. MM.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
Gene3Di1.25.40.20. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001357. BRCT_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF16770. RTT107_BRCT_5. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
SM00292. BRCT. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSLF1_MOUSE
AccessioniPrimary (citable) accession number: Q8R3P9
Secondary accession number(s): Q497V2
, Q5XK30, Q8BHY5, Q8BQM6, Q921Y9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: July 1, 2008
Last modified: November 30, 2016
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.