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Protein

Clusterin-associated protein 1

Gene

Cluap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for cilia biogenesis. Appears to function within the multiple intraflagellar transport complex B (IFT-B). Key regulator of hedgehog signaling.1 Publication

GO - Biological processi

  • axoneme assembly Source: MGI
  • cilium assembly Source: MGI
  • floor plate formation Source: MGI
  • heart looping Source: MGI
  • intraciliary transport Source: MGI
  • left/right pattern formation Source: MGI
  • neural tube closure Source: MGI
  • smoothened signaling pathway Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation

Enzyme and pathway databases

ReactomeiR-MMU-5620924. Intraflagellar transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Clusterin-associated protein 1
Gene namesi
Name:Cluap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1924029. Cluap1.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: MGI
  • ciliary base Source: MGI
  • ciliary tip Source: MGI
  • cilium Source: MGI
  • intracellular membrane-bounded organelle Source: MGI
  • intraciliary transport particle B Source: MGI
  • nucleoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Nucleus

Pathology & Biotechi

Disruption phenotypei

E9.5 embryos are still viable but die thereafter. Mutant embryos are runted, have exhibit enlarged pericardial sacs, and defects in neural tube development. There is a complete absence of cilia in E9.5 embryos. Sonic hedgehog (SHH) signaling is severely disrupted with down-regulation of PTCH1 and GLI1.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 413413Clusterin-associated protein 1PRO_0000239453Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei314 – 3141PhosphoserineCombined sources
Modified residuei324 – 3241PhosphoserineCombined sources
Modified residuei326 – 3261PhosphoserineCombined sources
Modified residuei409 – 4091PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8R3P7.
PaxDbiQ8R3P7.
PRIDEiQ8R3P7.

PTM databases

iPTMnetiQ8R3P7.
PhosphoSiteiQ8R3P7.

Expressioni

Tissue specificityi

Expressed in all tissues tested including heart, kidney, skeletal muscle, eye, liver, ovary, oviduct, testes, lung and brain. Elevated levels in multiciliated cells such as the bronchioles of the lungs, ependymal cells of the brain and cells with a single primary cilia of heart and kidney.1 Publication

Gene expression databases

BgeeiQ8R3P7.
CleanExiMM_CLUAP1.
ExpressionAtlasiQ8R3P7. baseline and differential.
GenevisibleiQ8R3P7. MM.

Interactioni

Subunit structurei

Interacts with CLU/clusterin.By similarity

Protein-protein interaction databases

BioGridi218308. 1 interaction.
STRINGi10090.ENSMUSP00000043397.

Structurei

3D structure databases

ProteinModelPortaliQ8R3P7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili185 – 308124Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi360 – 38627Asp-richAdd
BLAST

Sequence similaritiesi

Belongs to the CLUAP1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3647. Eukaryota.
ENOG410XRVS. LUCA.
GeneTreeiENSGT00390000008957.
HOGENOMiHOG000257815.
HOVERGENiHBG081253.
InParanoidiQ8R3P7.
KOiK19684.
OMAiMRIAVKD.
PhylomeDBiQ8R3P7.
TreeFamiTF314639.

Family and domain databases

InterProiIPR019366. Clusterin-associated_protein-1.
[Graphical view]
PANTHERiPTHR21547. PTHR21547. 1 hit.
PfamiPF10234. Cluap1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R3P7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFRDLRNFT EMMRALGYPR HISMENFRTP NFGLVSEVLL WLVKRYEPQT
60 70 80 90 100
DIPSDIETEQ DRVFFIKAIA QFMATKAHIK LNTKKLYQAD GYAVKELLKI
110 120 130 140 150
TSVLYNAMKT KGMEGSNVGE EDISKFKFDL GSKIADLKAA RQLASEITAK
160 170 180 190 200
GASLYDLLGK EVELRELRTE AIARPLEINE TEKVMRIAIK DLLAQVQKTK
210 220 230 240 250
DLLNNVASDE ANLEAKIEKR KLELERNRKR LQTLQSVRPA FMDEYEKVEE
260 270 280 290 300
DLQKQYDVYL EKFRNLAYLE QQLEDHHRME QERFEEAENT LRLMQNKLKE
310 320 330 340 350
EEKRLLKSGS NDDSDIDIQE DDESDSELED RRMSKPRTAM EVLMQGRPSK
360 370 380 390 400
RIVGAMQGGD SDEDEDSEDS EIDMEDDEED DDDLEDESIA LSPAKPSRRI
410
RKPEPLDESD NDF
Length:413
Mass (Da):47,953
Last modified:June 1, 2002 - v1
Checksum:iF0AEDF9320D09B0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK134648 mRNA. Translation: BAE22224.1.
BC024910 mRNA. Translation: AAH24910.1.
BC063769 mRNA. Translation: AAH63769.1.
CCDSiCCDS27912.1.
RefSeqiNP_084014.1. NM_029738.2.
UniGeneiMm.458921.

Genome annotation databases

EnsembliENSMUST00000040881; ENSMUSP00000043397; ENSMUSG00000014232.
GeneIDi76779.
KEGGimmu:76779.
UCSCiuc007xzb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK134648 mRNA. Translation: BAE22224.1.
BC024910 mRNA. Translation: AAH24910.1.
BC063769 mRNA. Translation: AAH63769.1.
CCDSiCCDS27912.1.
RefSeqiNP_084014.1. NM_029738.2.
UniGeneiMm.458921.

3D structure databases

ProteinModelPortaliQ8R3P7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218308. 1 interaction.
STRINGi10090.ENSMUSP00000043397.

PTM databases

iPTMnetiQ8R3P7.
PhosphoSiteiQ8R3P7.

Proteomic databases

MaxQBiQ8R3P7.
PaxDbiQ8R3P7.
PRIDEiQ8R3P7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040881; ENSMUSP00000043397; ENSMUSG00000014232.
GeneIDi76779.
KEGGimmu:76779.
UCSCiuc007xzb.1. mouse.

Organism-specific databases

CTDi23059.
MGIiMGI:1924029. Cluap1.

Phylogenomic databases

eggNOGiKOG3647. Eukaryota.
ENOG410XRVS. LUCA.
GeneTreeiENSGT00390000008957.
HOGENOMiHOG000257815.
HOVERGENiHBG081253.
InParanoidiQ8R3P7.
KOiK19684.
OMAiMRIAVKD.
PhylomeDBiQ8R3P7.
TreeFamiTF314639.

Enzyme and pathway databases

ReactomeiR-MMU-5620924. Intraflagellar transport.

Miscellaneous databases

ChiTaRSiCluap1. mouse.
PROiQ8R3P7.
SOURCEiSearch...

Gene expression databases

BgeeiQ8R3P7.
CleanExiMM_CLUAP1.
ExpressionAtlasiQ8R3P7. baseline and differential.
GenevisibleiQ8R3P7. MM.

Family and domain databases

InterProiIPR019366. Clusterin-associated_protein-1.
[Graphical view]
PANTHERiPTHR21547. PTHR21547. 1 hit.
PfamiPF10234. Cluap1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Medulla oblongata.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Jaw, Limb and Mammary tumor.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-409, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-314; SER-324; SER-326 AND SER-409, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney, Lung, Pancreas and Testis.
  5. "Mammalian Clusterin associated protein 1 is an evolutionarily conserved protein required for ciliogenesis."
    Pasek R.C., Berbari N.F., Lewis W.R., Kesterson R.A., Yoder B.K.
    Cilia 1:20-20(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiCLUA1_MOUSE
AccessioniPrimary (citable) accession number: Q8R3P7
Secondary accession number(s): Q6P3Y8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 1, 2002
Last modified: June 8, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.