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Protein

Probable E3 ubiquitin-protein ligase DTX2

Gene

Dtx2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Functions as a ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri409 – 47062RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • Notch signaling pathway Source: MGI
  • protein ubiquitination Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Notch signaling pathway, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-2122948. Activated NOTCH1 Transmits Signal to the Nucleus.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable E3 ubiquitin-protein ligase DTX2 (EC:6.3.2.-)
Alternative name(s):
Protein deltex-2
Short name:
Deltex2
Short name:
mDTX2
Gene namesi
Name:Dtx2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1921448. Dtx2.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Predominantly cytoplasmic. Partially nuclear.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

Recurrent site of retroviral integration in murine B-cell lymphomas.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 619619Probable E3 ubiquitin-protein ligase DTX2PRO_0000219084Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei248 – 2481N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8R3P2.
MaxQBiQ8R3P2.
PaxDbiQ8R3P2.
PRIDEiQ8R3P2.

PTM databases

iPTMnetiQ8R3P2.
PhosphoSiteiQ8R3P2.

Expressioni

Tissue specificityi

Expressed in testis and the CNS.1 Publication

Developmental stagei

In the CNS, it is expressed in the developing neural tube starting from E10.5 in the spinal cord and around E11.5 in the telencephalon. Expressed ubiquitously throughout the spinal cord and telencephalon during neurogenesis. Expressed throughout the developing retina at E15.5. Not expressed in the somite or presomite during somitogenesis. Expressed slightly earlier that Dtx1 and Dtx3.1 Publication

Gene expression databases

BgeeiQ8R3P2.
CleanExiMM_DTX2.
ExpressionAtlasiQ8R3P2. baseline and differential.
GenevisibleiQ8R3P2. MM.

Interactioni

Subunit structurei

Homodimer. May form a heterodimer with other members of the Deltex family. Interacts with NOTCH1.1 Publication

Protein-protein interaction databases

IntActiQ8R3P2. 1 interaction.
STRINGi10090.ENSMUSP00000106772.

Structurei

Secondary structure

1
619
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi390 – 3978Combined sources
Beta strandi398 – 4003Combined sources
Turni410 – 4134Combined sources
Turni422 – 4254Combined sources
Beta strandi429 – 4313Combined sources
Beta strandi437 – 4426Combined sources
Helixi448 – 45710Combined sources
Turni467 – 4693Combined sources
Beta strandi472 – 4754Combined sources
Beta strandi477 – 4793Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V87NMR-A389-489[»]
ProteinModelPortaliQ8R3P2.
SMRiQ8R3P2. Positions 19-182, 389-611.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8R3P2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 9791WWE 1PROSITE-ProRule annotationAdd
BLAST
Domaini98 – 17477WWE 2PROSITE-ProRule annotationAdd
BLAST

Domaini

The WWE domains are thought to mediate some protein-protein interaction, and are frequently found in ubiquitin ligases.By similarity

Sequence similaritiesi

Belongs to the Deltex family.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 2 WWE domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri409 – 47062RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IEMS. Eukaryota.
ENOG4111EY2. LUCA.
GeneTreeiENSGT00440000035370.
HOGENOMiHOG000007352.
HOVERGENiHBG007213.
InParanoidiQ8R3P2.
KOiK06058.
OMAiLSHAHFS.
OrthoDBiEOG7J17ZB.
PhylomeDBiQ8R3P2.
TreeFamiTF325526.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR004170. WWE-dom.
IPR018123. WWE-dom_subgr.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF02825. WWE. 2 hits.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
SM00678. WWE. 2 hits.
[Graphical view]
PROSITEiPS50918. WWE. 2 hits.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R3P2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAMAPSSSLP QVYPSHVVVA VWEWQDGLGI WHPYSATVCS FIEQHFVRQR
60 70 80 90 100
GQHFGLGSLA HSIPLGQADP SLAPYIIDLP SWTQFRQNTG TMRSVRRHLF
110 120 130 140 150
SQNSAPGQGI VWEWLGDDGS WVAYEARICD YLEQQVARGI QVVDLAPLGY
160 170 180 190 200
NYTVNYATLT QTNKTSSFCR SVRRQVGPVY PVTSDIAVPR QMGLICFCQQ
210 220 230 240 250
CLHGSGTGPV SGRYRHSMTN LPAYPAPQAP HRTTTVSGAH QAFAPYNKPS
260 270 280 290 300
LSGARSAPRL NTTNPWAAAP PVAGNQSLFH SSLSHLGPQL LPSGPSTSSG
310 320 330 340 350
ASASFPSGPS SSSPGSAPTT VPVQMPKASR VQQALAGMTS VLSAIGLPVC
360 370 380 390 400
LSRAPRPTGP PASRPASKSH SSVKRLRKMS VKEGAPKPEP EQVIRKYTEE
410 420 430 440 450
LKVAPEEDCI ICMEKLAVAS GYSDMTDSKA LGPMVVGRLT KCSHAFHLLC
460 470 480 490 500
LLAMYCNGNK DGSLQCPSCK TIYGEKTGTQ PWGKMEVFRF QMSLPGHEDC
510 520 530 540 550
GTILIVYNIP HGIQGPEHPS PGKPFTARGF PRQCYLPDSP QGRKVLELLK
560 570 580 590 600
VAWKRRLIFT VGTSSTTGET DTVVWNEIHH KTEMDRNVTG HGYPDPNYLQ
610
NVLAELAAQG VTEDCLEQQ
Length:619
Mass (Da):67,195
Last modified:September 26, 2003 - v2
Checksum:iA3A9F16F5B855697
GO
Isoform 2 (identifier: Q8R3P2-2) [UniParc]FASTAAdd to basket

Also known as: DeltaE

The sequence of this isoform differs from the canonical sequence as follows:
     337-382: Missing.

Show »
Length:573
Mass (Da):62,413
Checksum:iDAD97294AA8EDF28
GO
Isoform 3 (identifier: Q8R3P2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     338-357: MTSVLSAIGLPVCLSRAPRP → EDRRVYWLLVLYSGYRELDN
     358-619: Missing.

Note: Splicing donor site not canonical. No experimental confirmation available.
Show »
Length:357
Mass (Da):38,720
Checksum:iBB4C45686E15FED6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti383 – 3831Missing in BAB18940 (PubMed:11226752).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei337 – 38246Missing in isoform 2. 2 PublicationsVSP_008351Add
BLAST
Alternative sequencei338 – 35720MTSVL…RAPRP → EDRRVYWLLVLYSGYRELDN in isoform 3. 1 PublicationVSP_008352Add
BLAST
Alternative sequencei358 – 619262Missing in isoform 3. 1 PublicationVSP_008353Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015423 mRNA. Translation: BAB18940.1.
AB015424 mRNA. Translation: BAB18941.1.
AK012137 mRNA. Translation: BAB28055.1.
AK044105 mRNA. Translation: BAC31780.1.
AK054443 mRNA. Translation: BAC35781.1.
BC024925 mRNA. Translation: AAH24925.1.
CCDSiCCDS57389.1. [Q8R3P2-1]
CCDS57390.1. [Q8R3P2-2]
RefSeqiNP_001243025.1. NM_001256096.1. [Q8R3P2-1]
NP_001243026.1. NM_001256097.1. [Q8R3P2-2]
NP_001243027.1. NM_001256098.1. [Q8R3P2-2]
NP_076231.1. NM_023742.2.
XP_006504569.1. XM_006504506.2. [Q8R3P2-1]
XP_006504570.1. XM_006504507.2. [Q8R3P2-1]
XP_006504571.1. XM_006504508.1. [Q8R3P2-1]
XP_011239211.1. XM_011240909.1. [Q8R3P2-1]
UniGeneiMm.275574.

Genome annotation databases

EnsembliENSMUST00000111142; ENSMUSP00000106772; ENSMUSG00000004947. [Q8R3P2-1]
ENSMUST00000111144; ENSMUSP00000106774; ENSMUSG00000004947. [Q8R3P2-2]
ENSMUST00000111145; ENSMUSP00000106775; ENSMUSG00000004947. [Q8R3P2-2]
ENSMUST00000125827; ENSMUSP00000115122; ENSMUSG00000004947. [Q8R3P2-3]
GeneIDi74198.
KEGGimmu:74198.
UCSCiuc008zzm.2. mouse. [Q8R3P2-1]
uc008zzo.2. mouse. [Q8R3P2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015423 mRNA. Translation: BAB18940.1.
AB015424 mRNA. Translation: BAB18941.1.
AK012137 mRNA. Translation: BAB28055.1.
AK044105 mRNA. Translation: BAC31780.1.
AK054443 mRNA. Translation: BAC35781.1.
BC024925 mRNA. Translation: AAH24925.1.
CCDSiCCDS57389.1. [Q8R3P2-1]
CCDS57390.1. [Q8R3P2-2]
RefSeqiNP_001243025.1. NM_001256096.1. [Q8R3P2-1]
NP_001243026.1. NM_001256097.1. [Q8R3P2-2]
NP_001243027.1. NM_001256098.1. [Q8R3P2-2]
NP_076231.1. NM_023742.2.
XP_006504569.1. XM_006504506.2. [Q8R3P2-1]
XP_006504570.1. XM_006504507.2. [Q8R3P2-1]
XP_006504571.1. XM_006504508.1. [Q8R3P2-1]
XP_011239211.1. XM_011240909.1. [Q8R3P2-1]
UniGeneiMm.275574.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V87NMR-A389-489[»]
ProteinModelPortaliQ8R3P2.
SMRiQ8R3P2. Positions 19-182, 389-611.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8R3P2. 1 interaction.
STRINGi10090.ENSMUSP00000106772.

PTM databases

iPTMnetiQ8R3P2.
PhosphoSiteiQ8R3P2.

Proteomic databases

EPDiQ8R3P2.
MaxQBiQ8R3P2.
PaxDbiQ8R3P2.
PRIDEiQ8R3P2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000111142; ENSMUSP00000106772; ENSMUSG00000004947. [Q8R3P2-1]
ENSMUST00000111144; ENSMUSP00000106774; ENSMUSG00000004947. [Q8R3P2-2]
ENSMUST00000111145; ENSMUSP00000106775; ENSMUSG00000004947. [Q8R3P2-2]
ENSMUST00000125827; ENSMUSP00000115122; ENSMUSG00000004947. [Q8R3P2-3]
GeneIDi74198.
KEGGimmu:74198.
UCSCiuc008zzm.2. mouse. [Q8R3P2-1]
uc008zzo.2. mouse. [Q8R3P2-2]

Organism-specific databases

CTDi113878.
MGIiMGI:1921448. Dtx2.

Phylogenomic databases

eggNOGiENOG410IEMS. Eukaryota.
ENOG4111EY2. LUCA.
GeneTreeiENSGT00440000035370.
HOGENOMiHOG000007352.
HOVERGENiHBG007213.
InParanoidiQ8R3P2.
KOiK06058.
OMAiLSHAHFS.
OrthoDBiEOG7J17ZB.
PhylomeDBiQ8R3P2.
TreeFamiTF325526.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-MMU-2122948. Activated NOTCH1 Transmits Signal to the Nucleus.

Miscellaneous databases

ChiTaRSiDtx2. mouse.
EvolutionaryTraceiQ8R3P2.
PROiQ8R3P2.
SOURCEiSearch...

Gene expression databases

BgeeiQ8R3P2.
CleanExiMM_DTX2.
ExpressionAtlasiQ8R3P2. baseline and differential.
GenevisibleiQ8R3P2. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR004170. WWE-dom.
IPR018123. WWE-dom_subgr.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF02825. WWE. 2 hits.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
SM00678. WWE. 2 hits.
[Graphical view]
PROSITEiPS50918. WWE. 2 hits.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Murine homologs of deltex define a novel gene family involved in vertebrate Notch signaling and neurogenesis."
    Kishi N., Tang Z., Maeda Y., Hirai A., Mo R., Ito M., Suzuki S., Nakao K., Kinoshita T., Kadesch T., Hui C.-C., Artavanis-Tsakonas S., Okano H., Matsuno K.
    Int. J. Dev. Neurosci. 19:21-35(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MULTIMERIZATION, INTERACTION WITH NOTCH1.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Brain cortex, Embryo and Ovary.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Mammary tumor.
  4. Cited for: RETROVIRAL INSERTION.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Spleen.
  6. "Solution structure of the RING-H2 finger domain of mouse deltex protein 2."
    RIKEN structural genomics initiative (RSGI)
    Submitted (JUN-2004) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 389-489.

Entry informationi

Entry nameiDTX2_MOUSE
AccessioniPrimary (citable) accession number: Q8R3P2
Secondary accession number(s): Q9CZV3, Q9ER07, Q9ER08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: September 26, 2003
Last modified: June 8, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.