Q8R3P2 (DTX2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein deltex-2
Short name=Deltex2
Short name=mDTX2

Gene names

Name:

Dtx2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	619 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Functions as an ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity By similarity.
Subunit structure	Homomultimer. May form a heteromultimer with other members of the Deltex family. Interacts with NOTCH1. Ref.1
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: Predominantly cytoplasmic By similarity. Partially nuclear By similarity.
Tissue specificity	Expressed in testis and the CNS. Ref.1
Developmental stage	In the CNS, it is expressed in the developing neural tube starting from E10.5 in the spinal cord and around E11.5 in the telencephalon. Expressed ubiquitously throughout the spinal cord and telencephalon during neurogenesis. Expressed throughout the developing retina at E15.5. Not expressed in the somite or presomite during somitogenesis. Expressed slightly earlier that Dtx1 and Dtx3. Ref.1
Domain	The WWE domains are thought to mediate some protein-protein interaction, and are frequently found in ubiquitin ligases By similarity.
Involvement in disease	Note=Recurrent site of retroviral integration in murine B-cell lymphomas.
Sequence similarities	Belongs to the Deltex family. Contains 1 RING-type zinc finger. Contains 2 WWE domains.

Ontologies

Keywords
Biological process	Notch signaling pathway
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
PTM	Acetylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Notch signaling pathway Inferred from direct assay Ref.1. Source: MGI
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction Ref.1. Source: MGI zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8R3P2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8R3P2-2)

Also known as: DeltaE;

The sequence of this isoform differs from the canonical sequence as follows:
     337-382: Missing.

Isoform 3 (identifier: Q8R3P2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     338-357: MTSVLSAIGLPVCLSRAPRP → EDRRVYWLLVLYSGYRELDN
     358-619: Missing.

Note: Splicing donor site not canonical. No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 619

619

Protein deltex-2

PRO_0000219084

Regions

Domain

7 – 97

91

WWE 1

Domain

98 – 174

77

WWE 2

Zinc finger

409 – 470

62

RING-type

Amino acid modifications

Modified residue

248

1

N6-acetyllysine By similarity

Natural variations

Alternative sequence

337 – 382

46

Missing in isoform 2.

VSP_008351

Alternative sequence

338 – 357

20

MTSVL…RAPRP → EDRRVYWLLVLYSGYRELDN in isoform 3.

VSP_008352

Alternative sequence

358 – 619

262

Missing in isoform 3.

VSP_008353

Experimental info

Sequence conflict

383

1

Missing in BAB18940. Ref.1

Secondary structure

1

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619

Helix Strand Turn

Details...

Helix

390 – 397

8

Beta strand

398 – 400

3

Turn

410 – 413

4

Turn

422 – 425

4

Beta strand

429 – 431

3

Beta strand

437 – 442

6

Helix

448 – 457

10

Turn

467 – 469

3

Beta strand

472 – 475

4

Beta strand

477 – 479

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 26, 2003. Version 2. Checksum: A3A9F16F5B855697	FASTA	619	67,195
	10 20 30 40 50 60 MAMAPSSSLP QVYPSHVVVA VWEWQDGLGI WHPYSATVCS FIEQHFVRQR GQHFGLGSLA 70 80 90 100 110 120 HSIPLGQADP SLAPYIIDLP SWTQFRQNTG TMRSVRRHLF SQNSAPGQGI VWEWLGDDGS 130 140 150 160 170 180 WVAYEARICD YLEQQVARGI QVVDLAPLGY NYTVNYATLT QTNKTSSFCR SVRRQVGPVY 190 200 210 220 230 240 PVTSDIAVPR QMGLICFCQQ CLHGSGTGPV SGRYRHSMTN LPAYPAPQAP HRTTTVSGAH 250 260 270 280 290 300 QAFAPYNKPS LSGARSAPRL NTTNPWAAAP PVAGNQSLFH SSLSHLGPQL LPSGPSTSSG 310 320 330 340 350 360 ASASFPSGPS SSSPGSAPTT VPVQMPKASR VQQALAGMTS VLSAIGLPVC LSRAPRPTGP 370 380 390 400 410 420 PASRPASKSH SSVKRLRKMS VKEGAPKPEP EQVIRKYTEE LKVAPEEDCI ICMEKLAVAS 430 440 450 460 470 480 GYSDMTDSKA LGPMVVGRLT KCSHAFHLLC LLAMYCNGNK DGSLQCPSCK TIYGEKTGTQ 490 500 510 520 530 540 PWGKMEVFRF QMSLPGHEDC GTILIVYNIP HGIQGPEHPS PGKPFTARGF PRQCYLPDSP 550 560 570 580 590 600 QGRKVLELLK VAWKRRLIFT VGTSSTTGET DTVVWNEIHH KTEMDRNVTG HGYPDPNYLQ 610 NVLAELAAQG VTEDCLEQQ
	Isoform 2 (DeltaE) [UniParc]. Checksum: DAD97294AA8EDF28	FASTA	573	62,413
	10 20 30 40 50 60 MAMAPSSSLP QVYPSHVVVA VWEWQDGLGI WHPYSATVCS FIEQHFVRQR GQHFGLGSLA 70 80 90 100 110 120 HSIPLGQADP SLAPYIIDLP SWTQFRQNTG TMRSVRRHLF SQNSAPGQGI VWEWLGDDGS 130 140 150 160 170 180 WVAYEARICD YLEQQVARGI QVVDLAPLGY NYTVNYATLT QTNKTSSFCR SVRRQVGPVY 190 200 210 220 230 240 PVTSDIAVPR QMGLICFCQQ CLHGSGTGPV SGRYRHSMTN LPAYPAPQAP HRTTTVSGAH 250 260 270 280 290 300 QAFAPYNKPS LSGARSAPRL NTTNPWAAAP PVAGNQSLFH SSLSHLGPQL LPSGPSTSSG 310 320 330 340 350 360 ASASFPSGPS SSSPGSAPTT VPVQMPKASR VQQALAEGAP KPEPEQVIRK YTEELKVAPE 370 380 390 400 410 420 EDCIICMEKL AVASGYSDMT DSKALGPMVV GRLTKCSHAF HLLCLLAMYC NGNKDGSLQC 430 440 450 460 470 480 PSCKTIYGEK TGTQPWGKME VFRFQMSLPG HEDCGTILIV YNIPHGIQGP EHPSPGKPFT 490 500 510 520 530 540 ARGFPRQCYL PDSPQGRKVL ELLKVAWKRR LIFTVGTSST TGETDTVVWN EIHHKTEMDR 550 560 570 NVTGHGYPDP NYLQNVLAEL AAQGVTEDCL EQQ
	Isoform 3 [UniParc]. Checksum: BB4C45686E15FED6	FASTA	357	38,720
	10 20 30 40 50 60 MAMAPSSSLP QVYPSHVVVA VWEWQDGLGI WHPYSATVCS FIEQHFVRQR GQHFGLGSLA 70 80 90 100 110 120 HSIPLGQADP SLAPYIIDLP SWTQFRQNTG TMRSVRRHLF SQNSAPGQGI VWEWLGDDGS 130 140 150 160 170 180 WVAYEARICD YLEQQVARGI QVVDLAPLGY NYTVNYATLT QTNKTSSFCR SVRRQVGPVY 190 200 210 220 230 240 PVTSDIAVPR QMGLICFCQQ CLHGSGTGPV SGRYRHSMTN LPAYPAPQAP HRTTTVSGAH 250 260 270 280 290 300 QAFAPYNKPS LSGARSAPRL NTTNPWAAAP PVAGNQSLFH SSLSHLGPQL LPSGPSTSSG 310 320 330 340 350 ASASFPSGPS SSSPGSAPTT VPVQMPKASR VQQALAGEDR RVYWLLVLYS GYRELDN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Murine homologs of deltex define a novel gene family involved in vertebrate Notch signaling and neurogenesis." Kishi N., Tang Z., Maeda Y., Hirai A., Mo R., Ito M., Suzuki S., Nakao K., Kinoshita T., Kadesch T., Hui C.-C., Artavanis-Tsakonas S., Okano H., Matsuno K. Int. J. Dev. Neurosci. 19:21-35(2001) [PubMed: 11226752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MULTIMERIZATION, INTERACTION WITH NOTCH1.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Brain cortex, Embryo and Ovary.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Mammary tumor.
[4]	"New genes involved in cancer identified by retroviral tagging." Suzuki T., Shen H., Akagi K., Morse H.C. III, Malley J.D., Naiman D.Q., Jenkins N.A., Copeland N.G. Nat. Genet. 32:166-174(2002) [PubMed: 12185365] [Abstract] Cited for: RETROVIRAL INSERTION.
[5]	"Solution structure of the RING-H2 finger domain of mouse deltex protein 2." RIKEN structural genomics initiative (RSGI) Submitted (JUN-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 389-489.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB015423 mRNA. Translation: BAB18940.1.
AB015424 mRNA. Translation: BAB18941.1.
AK012137 mRNA. Translation: BAB28055.1.
AK044105 mRNA. Translation: BAC31780.1.
AK054443 mRNA. Translation: BAC35781.1.
BC024925 mRNA. Translation: AAH24925.1.

IPI

IPI00111429.
IPI00113171.
IPI00153781.

RefSeq

NP_076231.1. NM_023742.1.

UniGene

Mm.275574.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1V87	NMR	-	A	389-489	[»]

ProteinModelPortal

Q8R3P2.

SMR

Q8R3P2. Positions 16-184, 389-612.

ModBase

Search...

Protein-protein interaction databases

STRING

Q8R3P2.

PTM databases

PhosphoSite

Q8R3P2.

Proteomic databases

PRIDE

Q8R3P2.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000005072; ENSMUSP00000005072; ENSMUSG00000004947.
ENSMUST00000111141; ENSMUSP00000106771; ENSMUSG00000004947.
ENSMUST00000111142; ENSMUSP00000106772; ENSMUSG00000004947.
ENSMUST00000111144; ENSMUSP00000106774; ENSMUSG00000004947.
ENSMUST00000111145; ENSMUSP00000106775; ENSMUSG00000004947.
ENSMUST00000125827; ENSMUSP00000115122; ENSMUSG00000004947.

GeneID

74198.

KEGG

mmu:74198.

UCSC

uc008zzm.1. mouse.
uc008zzo.1. mouse.

Organism-specific databases

CTD

113878.

MGI

MGI:1921448. Dtx2.

Phylogenomic databases

eggNOG

maNOG06187.

GeneTree

ENSGT00440000035370.

HOGENOM

HBG446502.

HOVERGEN

HBG007213.

InParanoid

Q8R3P2.

OMA

YPVTTVI.

OrthoDB

EOG4XSKPV.

PhylomeDB

Q8R3P2.

Gene expression databases

ArrayExpress

Q8R3P2.

Bgee

Q8R3P2.

CleanEx

MM_DTX2.

Genevestigator

Q8R3P2.

GermOnline

ENSMUSG00000004947. Mus musculus.

Family and domain databases

InterPro

IPR004170. WWE-dom.
IPR018123. WWE-dom_subgr.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]

Gene3D

G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 1 hit.

KO

K06058.

Pfam

PF02825. WWE. 2 hits.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]

SMART

SM00184. RING. 1 hit.
SM00678. WWE. 2 hits.
[Graphical view]

PROSITE

PS50918. WWE. 2 hits.
PS00518. ZF_RING_1. False negative.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

340078.

SOURCE

Search...

Entry information

Entry name

DTX2_MOUSE

Accession

Primary (citable) accession number: Q8R3P2
Secondary accession number(s): Q9CZV3, Q9ER07, Q9ER08

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2003
Last sequence update:	September 26, 2003
Last modified:	November 16, 2011
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program