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Protein

Probable E3 ubiquitin-protein ligase DTX2

Gene

Dtx2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Functions as a ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri409 – 470RING-typePROSITE-ProRule annotationAdd BLAST62

GO - Molecular functioni

GO - Biological processi

  • Notch signaling pathway Source: MGI
  • protein ubiquitination Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Notch signaling pathway, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-2122948. Activated NOTCH1 Transmits Signal to the Nucleus.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable E3 ubiquitin-protein ligase DTX2 (EC:6.3.2.-)
Alternative name(s):
Protein deltex-2
Short name:
Deltex2
Short name:
mDTX2
Gene namesi
Name:Dtx2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1921448. Dtx2.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Predominantly cytoplasmic. Partially nuclear.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

Recurrent site of retroviral integration in murine B-cell lymphomas.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002190841 – 619Probable E3 ubiquitin-protein ligase DTX2Add BLAST619

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei213Asymmetric dimethylarginineBy similarity1
Modified residuei215Asymmetric dimethylarginineBy similarity1
Modified residuei232Asymmetric dimethylarginineBy similarity1
Modified residuei248N6-acetyllysineBy similarity1
Modified residuei255Omega-N-methylarginineBy similarity1

Keywords - PTMi

Acetylation, Methylation

Proteomic databases

PaxDbiQ8R3P2.
PRIDEiQ8R3P2.

PTM databases

iPTMnetiQ8R3P2.
PhosphoSitePlusiQ8R3P2.

Expressioni

Tissue specificityi

Expressed in testis and the CNS.1 Publication

Developmental stagei

In the CNS, it is expressed in the developing neural tube starting from E10.5 in the spinal cord and around E11.5 in the telencephalon. Expressed ubiquitously throughout the spinal cord and telencephalon during neurogenesis. Expressed throughout the developing retina at E15.5. Not expressed in the somite or presomite during somitogenesis. Expressed slightly earlier that Dtx1 and Dtx3.1 Publication

Gene expression databases

BgeeiENSMUSG00000004947.
CleanExiMM_DTX2.
ExpressionAtlasiQ8R3P2. baseline and differential.
GenevisibleiQ8R3P2. MM.

Interactioni

Subunit structurei

Homodimer. May form a heterodimer with other members of the Deltex family. Interacts with NOTCH1.1 Publication

Protein-protein interaction databases

IntActiQ8R3P2. 1 interactor.
STRINGi10090.ENSMUSP00000106772.

Structurei

Secondary structure

1619
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi390 – 397Combined sources8
Beta strandi398 – 400Combined sources3
Turni410 – 413Combined sources4
Turni422 – 425Combined sources4
Beta strandi429 – 431Combined sources3
Beta strandi437 – 442Combined sources6
Helixi448 – 457Combined sources10
Turni467 – 469Combined sources3
Beta strandi472 – 475Combined sources4
Beta strandi477 – 479Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V87NMR-A389-489[»]
ProteinModelPortaliQ8R3P2.
SMRiQ8R3P2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8R3P2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 97WWE 1PROSITE-ProRule annotationAdd BLAST91
Domaini98 – 174WWE 2PROSITE-ProRule annotationAdd BLAST77

Domaini

The WWE domains are thought to mediate some protein-protein interaction, and are frequently found in ubiquitin ligases.By similarity

Sequence similaritiesi

Belongs to the Deltex family.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 2 WWE domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri409 – 470RING-typePROSITE-ProRule annotationAdd BLAST62

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IEMS. Eukaryota.
ENOG4111EY2. LUCA.
GeneTreeiENSGT00440000035370.
HOGENOMiHOG000007352.
HOVERGENiHBG007213.
InParanoidiQ8R3P2.
KOiK06058.
OMAiQPQGKME.
OrthoDBiEOG091G04KU.
PhylomeDBiQ8R3P2.
TreeFamiTF325526.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR004170. WWE-dom.
IPR018123. WWE-dom_subgr.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF02825. WWE. 2 hits.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
SM00678. WWE. 2 hits.
[Graphical view]
PROSITEiPS50918. WWE. 2 hits.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R3P2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAMAPSSSLP QVYPSHVVVA VWEWQDGLGI WHPYSATVCS FIEQHFVRQR
60 70 80 90 100
GQHFGLGSLA HSIPLGQADP SLAPYIIDLP SWTQFRQNTG TMRSVRRHLF
110 120 130 140 150
SQNSAPGQGI VWEWLGDDGS WVAYEARICD YLEQQVARGI QVVDLAPLGY
160 170 180 190 200
NYTVNYATLT QTNKTSSFCR SVRRQVGPVY PVTSDIAVPR QMGLICFCQQ
210 220 230 240 250
CLHGSGTGPV SGRYRHSMTN LPAYPAPQAP HRTTTVSGAH QAFAPYNKPS
260 270 280 290 300
LSGARSAPRL NTTNPWAAAP PVAGNQSLFH SSLSHLGPQL LPSGPSTSSG
310 320 330 340 350
ASASFPSGPS SSSPGSAPTT VPVQMPKASR VQQALAGMTS VLSAIGLPVC
360 370 380 390 400
LSRAPRPTGP PASRPASKSH SSVKRLRKMS VKEGAPKPEP EQVIRKYTEE
410 420 430 440 450
LKVAPEEDCI ICMEKLAVAS GYSDMTDSKA LGPMVVGRLT KCSHAFHLLC
460 470 480 490 500
LLAMYCNGNK DGSLQCPSCK TIYGEKTGTQ PWGKMEVFRF QMSLPGHEDC
510 520 530 540 550
GTILIVYNIP HGIQGPEHPS PGKPFTARGF PRQCYLPDSP QGRKVLELLK
560 570 580 590 600
VAWKRRLIFT VGTSSTTGET DTVVWNEIHH KTEMDRNVTG HGYPDPNYLQ
610
NVLAELAAQG VTEDCLEQQ
Length:619
Mass (Da):67,195
Last modified:September 26, 2003 - v2
Checksum:iA3A9F16F5B855697
GO
Isoform 2 (identifier: Q8R3P2-2) [UniParc]FASTAAdd to basket
Also known as: DeltaE

The sequence of this isoform differs from the canonical sequence as follows:
     337-382: Missing.

Show »
Length:573
Mass (Da):62,413
Checksum:iDAD97294AA8EDF28
GO
Isoform 3 (identifier: Q8R3P2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     338-357: MTSVLSAIGLPVCLSRAPRP → EDRRVYWLLVLYSGYRELDN
     358-619: Missing.

Note: Splicing donor site not canonical. No experimental confirmation available.
Show »
Length:357
Mass (Da):38,720
Checksum:iBB4C45686E15FED6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti383Missing in BAB18940 (PubMed:11226752).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008351337 – 382Missing in isoform 2. 2 PublicationsAdd BLAST46
Alternative sequenceiVSP_008352338 – 357MTSVL…RAPRP → EDRRVYWLLVLYSGYRELDN in isoform 3. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_008353358 – 619Missing in isoform 3. 1 PublicationAdd BLAST262

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015423 mRNA. Translation: BAB18940.1.
AB015424 mRNA. Translation: BAB18941.1.
AK012137 mRNA. Translation: BAB28055.1.
AK044105 mRNA. Translation: BAC31780.1.
AK054443 mRNA. Translation: BAC35781.1.
BC024925 mRNA. Translation: AAH24925.1.
CCDSiCCDS57389.1. [Q8R3P2-1]
CCDS57390.1. [Q8R3P2-2]
RefSeqiNP_001243025.1. NM_001256096.1. [Q8R3P2-1]
NP_001243026.1. NM_001256097.1. [Q8R3P2-2]
NP_001243027.1. NM_001256098.1. [Q8R3P2-2]
NP_076231.1. NM_023742.2.
XP_006504569.1. XM_006504506.3. [Q8R3P2-1]
XP_006504570.1. XM_006504507.2. [Q8R3P2-1]
XP_006504571.1. XM_006504508.1. [Q8R3P2-1]
XP_017176609.1. XM_017321120.1. [Q8R3P2-2]
UniGeneiMm.275574.

Genome annotation databases

EnsembliENSMUST00000111142; ENSMUSP00000106772; ENSMUSG00000004947. [Q8R3P2-1]
ENSMUST00000111144; ENSMUSP00000106774; ENSMUSG00000004947. [Q8R3P2-2]
ENSMUST00000111145; ENSMUSP00000106775; ENSMUSG00000004947. [Q8R3P2-2]
ENSMUST00000125827; ENSMUSP00000115122; ENSMUSG00000004947. [Q8R3P2-3]
GeneIDi74198.
KEGGimmu:74198.
UCSCiuc008zzm.2. mouse. [Q8R3P2-1]
uc008zzo.2. mouse. [Q8R3P2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015423 mRNA. Translation: BAB18940.1.
AB015424 mRNA. Translation: BAB18941.1.
AK012137 mRNA. Translation: BAB28055.1.
AK044105 mRNA. Translation: BAC31780.1.
AK054443 mRNA. Translation: BAC35781.1.
BC024925 mRNA. Translation: AAH24925.1.
CCDSiCCDS57389.1. [Q8R3P2-1]
CCDS57390.1. [Q8R3P2-2]
RefSeqiNP_001243025.1. NM_001256096.1. [Q8R3P2-1]
NP_001243026.1. NM_001256097.1. [Q8R3P2-2]
NP_001243027.1. NM_001256098.1. [Q8R3P2-2]
NP_076231.1. NM_023742.2.
XP_006504569.1. XM_006504506.3. [Q8R3P2-1]
XP_006504570.1. XM_006504507.2. [Q8R3P2-1]
XP_006504571.1. XM_006504508.1. [Q8R3P2-1]
XP_017176609.1. XM_017321120.1. [Q8R3P2-2]
UniGeneiMm.275574.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V87NMR-A389-489[»]
ProteinModelPortaliQ8R3P2.
SMRiQ8R3P2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8R3P2. 1 interactor.
STRINGi10090.ENSMUSP00000106772.

PTM databases

iPTMnetiQ8R3P2.
PhosphoSitePlusiQ8R3P2.

Proteomic databases

PaxDbiQ8R3P2.
PRIDEiQ8R3P2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000111142; ENSMUSP00000106772; ENSMUSG00000004947. [Q8R3P2-1]
ENSMUST00000111144; ENSMUSP00000106774; ENSMUSG00000004947. [Q8R3P2-2]
ENSMUST00000111145; ENSMUSP00000106775; ENSMUSG00000004947. [Q8R3P2-2]
ENSMUST00000125827; ENSMUSP00000115122; ENSMUSG00000004947. [Q8R3P2-3]
GeneIDi74198.
KEGGimmu:74198.
UCSCiuc008zzm.2. mouse. [Q8R3P2-1]
uc008zzo.2. mouse. [Q8R3P2-2]

Organism-specific databases

CTDi113878.
MGIiMGI:1921448. Dtx2.

Phylogenomic databases

eggNOGiENOG410IEMS. Eukaryota.
ENOG4111EY2. LUCA.
GeneTreeiENSGT00440000035370.
HOGENOMiHOG000007352.
HOVERGENiHBG007213.
InParanoidiQ8R3P2.
KOiK06058.
OMAiQPQGKME.
OrthoDBiEOG091G04KU.
PhylomeDBiQ8R3P2.
TreeFamiTF325526.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-MMU-2122948. Activated NOTCH1 Transmits Signal to the Nucleus.

Miscellaneous databases

ChiTaRSiDtx2. mouse.
EvolutionaryTraceiQ8R3P2.
PROiQ8R3P2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000004947.
CleanExiMM_DTX2.
ExpressionAtlasiQ8R3P2. baseline and differential.
GenevisibleiQ8R3P2. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR004170. WWE-dom.
IPR018123. WWE-dom_subgr.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF02825. WWE. 2 hits.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
SM00678. WWE. 2 hits.
[Graphical view]
PROSITEiPS50918. WWE. 2 hits.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDTX2_MOUSE
AccessioniPrimary (citable) accession number: Q8R3P2
Secondary accession number(s): Q9CZV3, Q9ER07, Q9ER08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: September 26, 2003
Last modified: November 2, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.