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Protein

Transcription factor 25

Gene

Tcf25

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in cell death control. Acts as a transcriptional repressor. Has been shown to repress transcription of SRF in vitro and so may play a role in heart development (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor 25
Short name:
TCF-25
Alternative name(s):
Nuclear localized protein 1
Gene namesi
Name:Tcf25
Synonyms:D8Ertd325e, Nulp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1914105. Tcf25.

Subcellular locationi

  • Nucleus 2 Publications

  • Note: Some staining in the cytosol; cytosolic localization is reduced in staurosporine-induced cell death.

GO - Cellular componenti

  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 676676Transcription factor 25PRO_0000087266Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei602 – 6021PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8R3L2.
MaxQBiQ8R3L2.
PaxDbiQ8R3L2.
PRIDEiQ8R3L2.

PTM databases

iPTMnetiQ8R3L2.
PhosphoSiteiQ8R3L2.

Expressioni

Tissue specificityi

Broadly expressed in the embryo during the early stages of organogenesis with highest levels in dorsal root ganglia and little or no expression in liver and skin.1 Publication

Gene expression databases

BgeeiQ8R3L2.
CleanExiMM_TCF25.
GenevisibleiQ8R3L2. MM.

Interactioni

Subunit structurei

Interacts with XIAP.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000056485.

Structurei

3D structure databases

ProteinModelPortaliQ8R3L2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The C-terminal region mediates transcriptional repression.By similarity

Sequence similaritiesi

Belongs to the TCF25 family.Curated

Phylogenomic databases

eggNOGiKOG2422. Eukaryota.
ENOG410XQ1Q. LUCA.
GeneTreeiENSGT00390000005563.
HOVERGENiHBG070024.
InParanoidiQ8R3L2.
OMAiSTYMVTA.
OrthoDBiEOG7VMP5V.
PhylomeDBiQ8R3L2.
TreeFamiTF106155.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR006994. TCF25.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PANTHERiPTHR22684. PTHR22684. 1 hit.
PfamiPF04910. Tcf25. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R3L2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRRALRRLR GEQRGQEPLG PDALKFVLLD DDDAEEEGPK PGLGGRRPGG
60 70 80 90 100
AGKEGVRVNN RFELINTEDL EDDLVVNGER SDCTLPDSVS SGNKGRAKHG
110 120 130 140 150
NAETKQDGGA TKAGSSEQSN ASGKLRKKKK KQKNKKSCTG ESSENGLEDI
160 170 180 190 200
DRILERIEDS SGFSHPGPPP LSSRKHVLYV EHRHLNPDTE LKRYFGARAV
210 220 230 240 250
LGEQRPRQRQ RVYPKCTWLT TPKSTWPRYS KPGLSMRLLE SKKGLSFFAF
260 270 280 290 300
DHNEEYQQAQ HKFLVAVESM EPNNIVVLLQ TSPYHVDSLL QLSDACRFQE
310 320 330 340 350
DQEMARDLIE RALYSMECAF HPLFSLTSGT CRLDYRRPEN RSFYLTLYKQ
360 370 380 390 400
MSFLEKRGCP RTALEYCKLI LSLEPDEDPL CMLLLIDHLA LRARNYEYLI
410 420 430 440 450
RLFQEWEAHR NLSQLPNFAF SVPLAYFLLS QQTDLPEHEL SSARQQASLL
460 470 480 490 500
IQQALTMFPG VLMPLLEYCS VRPDATVSNH RFFGPDAEIS QPPALGQLVS
510 520 530 540 550
LYLGRSHFLW KEPAIMSWLE ENVHEVLQAV DAGDPAVEAC ENRRKVLYQR
560 570 580 590 600
APRNIHRHVI LSEIKEAVAA LPSDVTTQSV MGFDPLPPLD TIYSYVRPER
610 620 630 640 650
LSPVSHGNTI ALFFRSLLPN YTTEGERLEE GVAGGPNRNQ GLNRLMLAVR
660 670
DMMANFHFND LEVPREDNPE GEGDWD
Length:676
Mass (Da):76,685
Last modified:July 25, 2006 - v2
Checksum:i095539BBAA3E8535
GO
Isoform 2 (identifier: Q8R3L2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     625-625: G → L
     626-676: Missing.

Show »
Length:625
Mass (Da):70,947
Checksum:iA1D9660C0CC03B8D
GO
Isoform 3 (identifier: Q8R3L2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     54-68: Missing.
     625-626: GE → QL
     627-676: Missing.

Note: No experimental confirmation available.
Show »
Length:611
Mass (Da):69,303
Checksum:i6414B325F5E17E8E
GO
Isoform 4 (identifier: Q8R3L2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     119-143: Missing.
     625-625: G → L
     626-676: Missing.

Note: No experimental confirmation available.
Show »
Length:600
Mass (Da):68,213
Checksum:i37E46BB654EC8347
GO
Isoform 5 (identifier: Q8R3L2-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     625-626: GE → QL
     627-676: Missing.

Note: No experimental confirmation available.
Show »
Length:626
Mass (Da):71,075
Checksum:iB50029660C0CC03B
GO

Sequence cautioni

The sequence BAB28983.2 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAC32344.1 differs from that shown. Reason: Frameshift at position 598. Curated
The sequence BAC36220.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti129 – 1291K → E in BAE29956 (PubMed:16141072).Curated
Sequence conflicti129 – 1291K → E in BAE29977 (PubMed:16141072).Curated
Sequence conflicti129 – 1291K → E in BAE30150 (PubMed:16141072).Curated
Sequence conflicti129 – 1291K → E in BAE31009 (PubMed:16141072).Curated
Sequence conflicti129 – 1291K → E in BAE31265 (PubMed:16141072).Curated
Sequence conflicti176 – 1761H → L in AAG27133 (PubMed:12107429).Curated
Sequence conflicti204 – 2041Q → E in AAG27133 (PubMed:12107429).Curated
Sequence conflicti219 – 2191L → R in BAE39858 (PubMed:16141072).Curated
Sequence conflicti219 – 2191L → R in BAE40128 (PubMed:16141072).Curated
Sequence conflicti243 – 2431K → E in BAE28334 (PubMed:16141072).Curated
Sequence conflicti370 – 3701I → T in AAG27133 (PubMed:12107429).Curated
Sequence conflicti407 – 4071E → V in AAG27133 (PubMed:12107429).Curated
Sequence conflicti454 – 4541A → T in AAG27133 (PubMed:12107429).Curated
Sequence conflicti539 – 5391A → V in BAE28334 (PubMed:16141072).Curated
Sequence conflicti539 – 5391A → V in AAH25071 (PubMed:15489334).Curated
Sequence conflicti560 – 5601I → M in AAG27133 (PubMed:12107429).Curated
Sequence conflicti597 – 5971R → G in BAE39858 (PubMed:16141072).Curated
Sequence conflicti597 – 5971R → G in BAE40128 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei54 – 6815Missing in isoform 3. 1 PublicationVSP_020023Add
BLAST
Alternative sequencei119 – 14325Missing in isoform 4. 1 PublicationVSP_020024Add
BLAST
Alternative sequencei625 – 6262GE → QL in isoform 3 and isoform 5. 1 PublicationVSP_020025
Alternative sequencei625 – 6251G → L in isoform 2 and isoform 4. 3 PublicationsVSP_020026
Alternative sequencei626 – 67651Missing in isoform 2 and isoform 4. 3 PublicationsVSP_020027Add
BLAST
Alternative sequencei627 – 67650Missing in isoform 3 and isoform 5. 1 PublicationVSP_020028Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94988 mRNA. Translation: AAG27133.1.
AK013754 mRNA. Translation: BAB28983.2. Different initiation.
AK045397 mRNA. Translation: BAC32344.1. Frameshift.
AK076151 mRNA. Translation: BAC36220.1. Different initiation.
AK088360 mRNA. Translation: BAC40301.1.
AK089964 mRNA. Translation: BAC41018.1.
AK148082 mRNA. Translation: BAE28334.1.
AK150232 mRNA. Translation: BAE29397.1.
AK150918 mRNA. Translation: BAE29956.1.
AK150942 mRNA. Translation: BAE29977.1.
AK151143 mRNA. Translation: BAE30150.1.
AK152178 mRNA. Translation: BAE31009.1.
AK152494 mRNA. Translation: BAE31265.1.
AK161627 mRNA. Translation: BAE36500.1.
AK167837 mRNA. Translation: BAE39858.1.
AK168167 mRNA. Translation: BAE40128.1.
BC025071 mRNA. Translation: AAH25071.1.
BC046768 mRNA. Translation: AAH46768.2.
CCDSiCCDS22755.1. [Q8R3L2-1]
CCDS52700.1. [Q8R3L2-5]
RefSeqiNP_001032966.1. NM_001037877.3. [Q8R3L2-5]
NP_001032967.1. NM_001037878.3. [Q8R3L2-1]
NP_001273291.1. NM_001286362.1. [Q8R3L2-4]
NP_001273292.1. NM_001286363.1.
NP_080080.2. NM_025804.3. [Q8R3L2-2]
UniGeneiMm.178818.

Genome annotation databases

EnsembliENSMUST00000057934; ENSMUSP00000056485; ENSMUSG00000001472. [Q8R3L2-1]
ENSMUST00000108840; ENSMUSP00000104468; ENSMUSG00000001472. [Q8R3L2-5]
GeneIDi66855.
KEGGimmu:66855.
UCSCiuc009nvl.2. mouse. [Q8R3L2-1]
uc009nvn.2. mouse. [Q8R3L2-2]
uc009nvo.2. mouse. [Q8R3L2-4]
uc009nvp.2. mouse. [Q8R3L2-5]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94988 mRNA. Translation: AAG27133.1.
AK013754 mRNA. Translation: BAB28983.2. Different initiation.
AK045397 mRNA. Translation: BAC32344.1. Frameshift.
AK076151 mRNA. Translation: BAC36220.1. Different initiation.
AK088360 mRNA. Translation: BAC40301.1.
AK089964 mRNA. Translation: BAC41018.1.
AK148082 mRNA. Translation: BAE28334.1.
AK150232 mRNA. Translation: BAE29397.1.
AK150918 mRNA. Translation: BAE29956.1.
AK150942 mRNA. Translation: BAE29977.1.
AK151143 mRNA. Translation: BAE30150.1.
AK152178 mRNA. Translation: BAE31009.1.
AK152494 mRNA. Translation: BAE31265.1.
AK161627 mRNA. Translation: BAE36500.1.
AK167837 mRNA. Translation: BAE39858.1.
AK168167 mRNA. Translation: BAE40128.1.
BC025071 mRNA. Translation: AAH25071.1.
BC046768 mRNA. Translation: AAH46768.2.
CCDSiCCDS22755.1. [Q8R3L2-1]
CCDS52700.1. [Q8R3L2-5]
RefSeqiNP_001032966.1. NM_001037877.3. [Q8R3L2-5]
NP_001032967.1. NM_001037878.3. [Q8R3L2-1]
NP_001273291.1. NM_001286362.1. [Q8R3L2-4]
NP_001273292.1. NM_001286363.1.
NP_080080.2. NM_025804.3. [Q8R3L2-2]
UniGeneiMm.178818.

3D structure databases

ProteinModelPortaliQ8R3L2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000056485.

PTM databases

iPTMnetiQ8R3L2.
PhosphoSiteiQ8R3L2.

Proteomic databases

EPDiQ8R3L2.
MaxQBiQ8R3L2.
PaxDbiQ8R3L2.
PRIDEiQ8R3L2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000057934; ENSMUSP00000056485; ENSMUSG00000001472. [Q8R3L2-1]
ENSMUST00000108840; ENSMUSP00000104468; ENSMUSG00000001472. [Q8R3L2-5]
GeneIDi66855.
KEGGimmu:66855.
UCSCiuc009nvl.2. mouse. [Q8R3L2-1]
uc009nvn.2. mouse. [Q8R3L2-2]
uc009nvo.2. mouse. [Q8R3L2-4]
uc009nvp.2. mouse. [Q8R3L2-5]

Organism-specific databases

CTDi22980.
MGIiMGI:1914105. Tcf25.

Phylogenomic databases

eggNOGiKOG2422. Eukaryota.
ENOG410XQ1Q. LUCA.
GeneTreeiENSGT00390000005563.
HOVERGENiHBG070024.
InParanoidiQ8R3L2.
OMAiSTYMVTA.
OrthoDBiEOG7VMP5V.
PhylomeDBiQ8R3L2.
TreeFamiTF106155.

Miscellaneous databases

ChiTaRSiTcf25. mouse.
PROiQ8R3L2.
SOURCEiSearch...

Gene expression databases

BgeeiQ8R3L2.
CleanExiMM_TCF25.
GenevisibleiQ8R3L2. MM.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR006994. TCF25.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PANTHERiPTHR22684. PTHR22684. 1 hit.
PfamiPF04910. Tcf25. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nulp1, a novel basic helix-loop-helix protein expressed broadly during early embryonic organogenesis and prominently in developing dorsal root ganglia."
    Olsson M., Durbeej M., Ekblom P., Hjalt T.
    Cell Tissue Res. 308:361-370(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Embryonic kidney.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 3; 4 AND 5), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 51-676 (ISOFORM 2).
    Strain: BALB/cJ, C57BL/6J and NOD.
    Tissue: Bone marrow, Corpora quadrigemina, Melanoma, Spleen and Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and FVB/N.
    Tissue: Brain and Mammary gland.
  4. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  5. "Nuclear localized protein-1 (Nulp1) increases cell death of human osteosarcoma cells and binds the X-linked inhibitor of apoptosis protein."
    Steen H., Lindholm D.
    Biochem. Biophys. Res. Commun. 366:432-437(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH XIAP.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Pancreas.

Entry informationi

Entry nameiTCF25_MOUSE
AccessioniPrimary (citable) accession number: Q8R3L2
Secondary accession number(s): Q3THR8
, Q3UBI7, Q3UD64, Q3UG75, Q80ZX3, Q8BR80, Q8C200, Q8C2M3, Q8C6B4, Q9CUW0, Q9ER19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: July 25, 2006
Last modified: June 8, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.