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Protein

Glutathione-specific gamma-glutamylcyclotransferase 1

Gene

Chac1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the cleavage glutathione into 5-oxoproline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. Glutathione depletion is an important factor for apoptosis initiation and execution. Acts as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade (By similarity). Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos (PubMed:22445366).By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei116 – 1161Proton acceptorBy similarity

GO - Molecular functioni

  • Notch binding Source: UniProtKB
  • transferase activity, transferring acyl groups Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Apoptosis, Neurogenesis, Notch signaling pathway, Unfolded protein response

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione-specific gamma-glutamylcyclotransferase 1By similarity (EC:2.3.2.-By similarity)
Short name:
Gamma-GCG 1By similarity
Alternative name(s):
Blocks Notch protein1 Publication
Short name:
Botch1 Publication
Cation transport regulator-like protein 1By similarity
Gene namesi
Name:Chac1By similarity
Synonyms:Botch1 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1916315. Chac1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • trans-Golgi network Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 223223Glutathione-specific gamma-glutamylcyclotransferase 1PRO_0000239011Add
BLAST

Proteomic databases

PRIDEiQ8R3J5.

PTM databases

PhosphoSiteiQ8R3J5.

Expressioni

Tissue specificityi

Widely expressed, with high expression in forebrain and anterior spinal cord. Expressed at intermediate level in the dorsal aorta and heart. Present throughout adult brain (at protein level).1 Publication

Gene expression databases

BgeeiQ8R3J5.
CleanExiMM_CHAC1.
GenevisibleiQ8R3J5. MM.

Interactioni

Subunit structurei

Interacts with NOTCH1 (via extracellular region).1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028780.

Structurei

3D structure databases

ProteinModelPortaliQ8R3J5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni36 – 416Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3703.
GeneTreeiENSGT00390000003855.
HOGENOMiHOG000264264.
HOVERGENiHBG056733.
InParanoidiQ8R3J5.
KOiK07232.
OMAiGTMKQES.
OrthoDBiEOG7F7W9F.
TreeFamiTF313048.

Family and domain databases

Gene3Di3.10.490.10. 1 hit.
InterProiIPR013024. Butirosin_synth_BtrG-like.
IPR006840. ChaC.
[Graphical view]
PANTHERiPTHR12192. PTHR12192. 1 hit.
PfamiPF04752. ChaC. 1 hit.
[Graphical view]
SUPFAMiSSF110857. SSF110857. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8R3J5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQESASQST PPPSLSPAPS SAQPSWGDGD PQALWIFGYG SLVWKPDFAY
60 70 80 90 100
SDSRVGFVRG YSRRFWQGDT FHRGSDKMPG RVVTLLEDRE GCTWGVAYQV
110 120 130 140 150
RGEQVNEALK YLNVREAVLG GYDTKEVTFY PQDTPDQPLT ALAYVATPQN
160 170 180 190 200
PGYLGPAPEE VIATQILACR GFSGHNLEYL LRLADFMQLC GPQAQDEHLE
210 220
AIVDAVGTLL PCSYLPEQPL ALT
Length:223
Mass (Da):24,547
Last modified:June 13, 2012 - v2
Checksum:i2DF793150C1C3B67
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti25 – 251S → F in AAH25169 (PubMed:15489334).Curated
Sequence conflicti89 – 891R → H in AAH25169 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007378 mRNA. Translation: BAB24997.1.
AK032218 mRNA. Translation: BAC27764.1.
AL929318 Genomic DNA. Translation: CAM25343.1.
BC025169 mRNA. Translation: AAH25169.1.
CCDSiCCDS16601.1.
RefSeqiNP_081205.1. NM_026929.4.
UniGeneiMm.35083.

Genome annotation databases

EnsembliENSMUST00000028780; ENSMUSP00000028780; ENSMUSG00000027313.
GeneIDi69065.
KEGGimmu:69065.
UCSCiuc008lts.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007378 mRNA. Translation: BAB24997.1.
AK032218 mRNA. Translation: BAC27764.1.
AL929318 Genomic DNA. Translation: CAM25343.1.
BC025169 mRNA. Translation: AAH25169.1.
CCDSiCCDS16601.1.
RefSeqiNP_081205.1. NM_026929.4.
UniGeneiMm.35083.

3D structure databases

ProteinModelPortaliQ8R3J5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028780.

PTM databases

PhosphoSiteiQ8R3J5.

Proteomic databases

PRIDEiQ8R3J5.

Protocols and materials databases

DNASUi69065.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028780; ENSMUSP00000028780; ENSMUSG00000027313.
GeneIDi69065.
KEGGimmu:69065.
UCSCiuc008lts.3. mouse.

Organism-specific databases

CTDi79094.
MGIiMGI:1916315. Chac1.

Phylogenomic databases

eggNOGiCOG3703.
GeneTreeiENSGT00390000003855.
HOGENOMiHOG000264264.
HOVERGENiHBG056733.
InParanoidiQ8R3J5.
KOiK07232.
OMAiGTMKQES.
OrthoDBiEOG7F7W9F.
TreeFamiTF313048.

Miscellaneous databases

NextBioi328512.
PROiQ8R3J5.
SOURCEiSearch...

Gene expression databases

BgeeiQ8R3J5.
CleanExiMM_CHAC1.
GenevisibleiQ8R3J5. MM.

Family and domain databases

Gene3Di3.10.490.10. 1 hit.
InterProiIPR013024. Butirosin_synth_BtrG-like.
IPR006840. ChaC.
[Graphical view]
PANTHERiPTHR12192. PTHR12192. 1 hit.
PfamiPF04752. ChaC. 1 hit.
[Graphical view]
SUPFAMiSSF110857. SSF110857. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Olfactory bulb and Pancreas.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH NOTCH1.

Entry informationi

Entry nameiCHAC1_MOUSE
AccessioniPrimary (citable) accession number: Q8R3J5
Secondary accession number(s): A2AV63, Q9D944
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: June 13, 2012
Last modified: July 22, 2015
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.