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Protein

Glutathione-specific gamma-glutamylcyclotransferase 1

Gene

Chac1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. Glutathione depletion is an important factor for apoptosis initiation and execution. Acts as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade (By similarity). Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos (PubMed:22445366).By similarity2 Publications

Catalytic activityi

Glutathione = L-cysteinylglycine + 5-oxo-L-proline.1 Publication

Kineticsi

  1. KM=3.13 mM for glutathione1 Publication
  1. Vmax=980 µmol/h/mg enzyme1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei116Proton acceptorBy similarity1

GO - Molecular functioni

  • gamma-glutamylcyclotransferase activity Source: GO_Central
  • lyase activity Source: UniProtKB-KW
  • Notch binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processApoptosis, Neurogenesis, Notch signaling pathway, Unfolded protein response

Enzyme and pathway databases

ReactomeiR-MMU-174403 Glutathione synthesis and recycling

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione-specific gamma-glutamylcyclotransferase 11 Publication (EC:4.3.2.71 Publication)
Short name:
Gamma-GCG 11 Publication
Alternative name(s):
Blocks Notch protein1 Publication
Short name:
Botch1 Publication
Cation transport regulator-like protein 1By similarity
Gene namesi
Name:Chac1By similarity
Synonyms:Botch1 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1916315 Chac1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi116E → Q: Loss of catalytic activity against glutathione. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002390111 – 223Glutathione-specific gamma-glutamylcyclotransferase 1Add BLAST223

Proteomic databases

PaxDbiQ8R3J5
PRIDEiQ8R3J5

PTM databases

PhosphoSitePlusiQ8R3J5

Expressioni

Tissue specificityi

Widely expressed, with high expression in forebrain and anterior spinal cord. Expressed at intermediate level in the dorsal aorta and heart. Present throughout adult brain (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000027313
CleanExiMM_CHAC1
GenevisibleiQ8R3J5 MM

Interactioni

Subunit structurei

Interacts with NOTCH1 (via extracellular region).1 Publication

GO - Molecular functioni

  • Notch binding Source: UniProtKB

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028780

Structurei

3D structure databases

ProteinModelPortaliQ8R3J5
SMRiQ8R3J5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni36 – 41Substrate bindingBy similarity6

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3182 Eukaryota
COG3703 LUCA
GeneTreeiENSGT00390000003855
HOGENOMiHOG000264264
HOVERGENiHBG056733
InParanoidiQ8R3J5
KOiK07232
OMAiTWGVAFE
OrthoDBiEOG091G0MZM
TreeFamiTF313048

Family and domain databases

CDDicd06661 GGCT_like, 1 hit
InterProiView protein in InterPro
IPR006840 ChaC
IPR013024 GGCT-like
IPR036568 GGCT-like_sf
PANTHERiPTHR12192 PTHR12192, 1 hit
PfamiView protein in Pfam
PF04752 ChaC, 1 hit
SUPFAMiSSF110857 SSF110857, 1 hit

Sequencei

Sequence statusi: Complete.

Q8R3J5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQESASQST PPPSLSPAPS SAQPSWGDGD PQALWIFGYG SLVWKPDFAY
60 70 80 90 100
SDSRVGFVRG YSRRFWQGDT FHRGSDKMPG RVVTLLEDRE GCTWGVAYQV
110 120 130 140 150
RGEQVNEALK YLNVREAVLG GYDTKEVTFY PQDTPDQPLT ALAYVATPQN
160 170 180 190 200
PGYLGPAPEE VIATQILACR GFSGHNLEYL LRLADFMQLC GPQAQDEHLE
210 220
AIVDAVGTLL PCSYLPEQPL ALT
Length:223
Mass (Da):24,547
Last modified:June 13, 2012 - v2
Checksum:i2DF793150C1C3B67
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25S → F in AAH25169 (PubMed:15489334).Curated1
Sequence conflicti89R → H in AAH25169 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007378 mRNA Translation: BAB24997.1
AK032218 mRNA Translation: BAC27764.1
AL929318 Genomic DNA No translation available.
BC025169 mRNA Translation: AAH25169.1
CCDSiCCDS16601.1
RefSeqiNP_081205.1, NM_026929.4
UniGeneiMm.35083

Genome annotation databases

EnsembliENSMUST00000028780; ENSMUSP00000028780; ENSMUSG00000027313
GeneIDi69065
KEGGimmu:69065
UCSCiuc008lts.3 mouse

Similar proteinsi

Entry informationi

Entry nameiCHAC1_MOUSE
AccessioniPrimary (citable) accession number: Q8R3J5
Secondary accession number(s): A2AV63, Q9D944
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: June 13, 2012
Last modified: March 28, 2018
This is version 101 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health