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Protein

UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8

Gene

B3gnt8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Beta-1,3-N-acetylglucosaminyltransferase that plays a role in the elongation of specific branch structures of multiantennary N-glycans. Has strong activity towards tetraantennary N-glycans and 2,6 triantennary glycans (By similarity).By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.Curated
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-913709. O-linked glycosylation of mucins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8 (EC:2.4.1.-)
Short name:
BGnT-8
Short name:
Beta-1,3-Gn-T8
Short name:
Beta-1,3-N-acetylglucosaminyltransferase 8
Short name:
Beta3Gn-T8
Gene namesi
Name:B3gnt8Imported
Synonyms:B3galt7Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2385269. B3gnt8.

Subcellular locationi

  • Golgi apparatus membrane By similarity; Single-pass type II membrane protein By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Transmembranei8 – 24Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
Topological domaini25 – 389LumenalSequence analysisAdd BLAST365

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003063821 – 389UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8Add BLAST389

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi55N-linked (GlcNAc...)Sequence analysis1
Glycosylationi212N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8R3I9.
PaxDbiQ8R3I9.
PRIDEiQ8R3I9.

PTM databases

PhosphoSitePlusiQ8R3I9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000059479.
ExpressionAtlasiQ8R3I9. baseline and differential.
GenevisibleiQ8R3I9. MM.

Interactioni

Subunit structurei

Interacts with B3GNT2; this interaction greatly increases B3GNT2 catalytic activity, independently of B3GNT8 enzymatic activity.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000092277.

Structurei

3D structure databases

ProteinModelPortaliQ8R3I9.
SMRiQ8R3I9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi218 – 221Poly-LeuSequence analysis4

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Sequence analysis

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2287. Eukaryota.
ENOG410ZZ1B. LUCA.
GeneTreeiENSGT00760000118879.
HOGENOMiHOG000232195.
HOVERGENiHBG050653.
InParanoidiQ8R3I9.
KOiK09665.
OMAiYIEWTSE.
OrthoDBiEOG091G0AXM.
PhylomeDBiQ8R3I9.
TreeFamiTF318639.

Family and domain databases

InterProiIPR002659. Glyco_trans_31.
[Graphical view]
PANTHERiPTHR11214. PTHR11214. 1 hit.
PfamiPF01762. Galactosyl_T. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R3I9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRCRKCQLCL SALLTLLGLK VYIEWTSESW LKKAEPRGAL PSPTPPNAEP
60 70 80 90 100
TLPTNLSARL GQTGPLSSAY WNQQQRQLGV LPSTDCQTWG TVAASEILDF
110 120 130 140 150
ILYPQELRRF LLSAACRSFP LWLPAGEGSP VASCSDKDVP YLLLAVKSEP
160 170 180 190 200
GHFAARQAVR ETWGSPVAGT RLLFLLGSPL GMGGPDLRSL VTWESRRYGD
210 220 230 240 250
LLLWDFLDVP YNRTLKDLLL LTWLSHHCPD VNFVLQVQDD AFVHIPALLE
260 270 280 290 300
HLQTLPPTWA RSLYLGEIFT QAKPLRKPGG PFYVPKTFFE GDYPAYASGG
310 320 330 340 350
GYVISGRLAP WLLQAAARVA PFPFDDVYTG FCFRALGLAP RAHPGFLTAW
360 370 380
PAERTRDPCA VRGLLLVHPV SPQDTIWLWR HLWVPELQC
Length:389
Mass (Da):43,370
Last modified:June 1, 2002 - v1
Checksum:i62ACD86829600D1C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK042388 mRNA. Translation: BAC31246.1.
AK154220 mRNA. Translation: BAE32443.1.
AK154901 mRNA. Translation: BAE32912.1.
BC025206 mRNA. Translation: AAH25206.1.
CCDSiCCDS20989.1.
RefSeqiNP_001031817.1. NM_001036740.2.
NP_666296.1. NM_146184.4.
XP_006539916.1. XM_006539853.3.
UniGeneiMm.192369.

Genome annotation databases

EnsembliENSMUST00000076034; ENSMUSP00000092277; ENSMUSG00000059479.
ENSMUST00000206940; ENSMUSP00000145797; ENSMUSG00000059479.
GeneIDi232984.
KEGGimmu:232984.
UCSCiuc009ftg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK042388 mRNA. Translation: BAC31246.1.
AK154220 mRNA. Translation: BAE32443.1.
AK154901 mRNA. Translation: BAE32912.1.
BC025206 mRNA. Translation: AAH25206.1.
CCDSiCCDS20989.1.
RefSeqiNP_001031817.1. NM_001036740.2.
NP_666296.1. NM_146184.4.
XP_006539916.1. XM_006539853.3.
UniGeneiMm.192369.

3D structure databases

ProteinModelPortaliQ8R3I9.
SMRiQ8R3I9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000092277.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

PTM databases

PhosphoSitePlusiQ8R3I9.

Proteomic databases

MaxQBiQ8R3I9.
PaxDbiQ8R3I9.
PRIDEiQ8R3I9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000076034; ENSMUSP00000092277; ENSMUSG00000059479.
ENSMUST00000206940; ENSMUSP00000145797; ENSMUSG00000059479.
GeneIDi232984.
KEGGimmu:232984.
UCSCiuc009ftg.1. mouse.

Organism-specific databases

CTDi374907.
MGIiMGI:2385269. B3gnt8.

Phylogenomic databases

eggNOGiKOG2287. Eukaryota.
ENOG410ZZ1B. LUCA.
GeneTreeiENSGT00760000118879.
HOGENOMiHOG000232195.
HOVERGENiHBG050653.
InParanoidiQ8R3I9.
KOiK09665.
OMAiYIEWTSE.
OrthoDBiEOG091G0AXM.
PhylomeDBiQ8R3I9.
TreeFamiTF318639.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-MMU-913709. O-linked glycosylation of mucins.

Miscellaneous databases

ChiTaRSiB3gnt8. mouse.
PROiQ8R3I9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000059479.
ExpressionAtlasiQ8R3I9. baseline and differential.
GenevisibleiQ8R3I9. MM.

Family and domain databases

InterProiIPR002659. Glyco_trans_31.
[Graphical view]
PANTHERiPTHR11214. PTHR11214. 1 hit.
PfamiPF01762. Galactosyl_T. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiB3GN8_MOUSE
AccessioniPrimary (citable) accession number: Q8R3I9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: June 1, 2002
Last modified: November 30, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.