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Protein

Lysophospholipid acyltransferase 2

Gene

Mboat2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) (LPCAT activity). To a lesser extent, also catalyzes the acylation of lysophosphatidylethanolamine (1-acyl-sn-glycero-3-phosphoethanolamine or LPE) into phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine or PE) (LPEAT activity). Prefers oleoyl-CoA as the acyl donor. Lysophospholipid acyltransferases (LPLATs) catalyze the reacylation step of the phospholipid remodeling pathway also known as the Lands cycle.1 Publication

Catalytic activityi

Acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine = CoA + 1,2-diacyl-sn-glycero-3-phosphocholine.
Acyl-CoA + 1-acyl-sn-glycero-3-phosphoethanolamine = CoA + 1,2-diacyl-sn-glycero-3-phosphoethanolamine.

Kineticsi

  1. KM=5.3 µM for oleoyl-CoA (in the presence of LPC C16:0 as cosubstrate)1 Publication
  2. KM=48.15 µM for oleoyl-CoA (in the presence of LPE C16:0 as cosubstrate)1 Publication
  3. KM=7.9 µM for LPC C16:0 (in the presence of oleoyl-CoA as cosubstrate)1 Publication
  4. KM=27.7 µM for LPE C16:0 (in the presence of oleoyl-CoA as cosubstrate)1 Publication
  1. Vmax=24.24 nmol/min/mg enzyme with oleoyl-CoA and LPC C16:0 as substrates1 Publication
  2. Vmax=26.3 nmol/min/mg enzyme with oleoyl-CoA and LPE C16:0 as substrates1 Publication

Pathwayi: phospholipid metabolism

This protein is involved in the pathway phospholipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway phospholipid metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei341By similarity1
Active sitei372By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BRENDAi2.3.1.23. 3474.
ReactomeiR-MMU-1482788. Acyl chain remodelling of PC.
R-MMU-1482839. Acyl chain remodelling of PE.
UniPathwayiUPA00085.

Chemistry databases

SwissLipidsiSLP:000000287.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysophospholipid acyltransferase 2 (EC:2.3.1.-)
Short name:
LPLAT 2
Alternative name(s):
1-acylglycerophosphocholine O-acyltransferase (EC:2.3.1.23)
1-acylglycerophosphoethanolamine O-acyltransferase (EC:2.3.1.n7)
Lysophosphatidylcholine acyltransferase
Short name:
LPCAT
Short name:
Lyso-PC acyltransferase
Lysophosphatidylcholine acyltransferase 4
Short name:
Lyso-PC acyltransferase 4
Short name:
mLPCAT4
Lysophosphatidylethanolamine acyltransferase
Short name:
LPEAT
Short name:
Lyso-PE acyltransferase
Membrane-bound O-acyltransferase domain-containing protein 2
Short name:
O-acyltransferase domain-containing protein 2
Gene namesi
Name:Mboat2
Synonyms:Lpcat4, Oact2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1914466. Mboat2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei22 – 42HelicalSequence analysisAdd BLAST21
Transmembranei61 – 81HelicalSequence analysisAdd BLAST21
Transmembranei88 – 108HelicalSequence analysisAdd BLAST21
Transmembranei184 – 204HelicalSequence analysisAdd BLAST21
Transmembranei236 – 256HelicalSequence analysisAdd BLAST21
Transmembranei263 – 283HelicalSequence analysisAdd BLAST21
Transmembranei365 – 385HelicalSequence analysisAdd BLAST21
Transmembranei415 – 435HelicalSequence analysisAdd BLAST21
Transmembranei443 – 463HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1255156.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002730211 – 519Lysophospholipid acyltransferase 2Add BLAST519

Proteomic databases

PaxDbiQ8R3I2.
PeptideAtlasiQ8R3I2.
PRIDEiQ8R3I2.

PTM databases

iPTMnetiQ8R3I2.
PhosphoSitePlusiQ8R3I2.

Expressioni

Tissue specificityi

Highly expressed in epididymis, brain, testis, and ovary.1 Publication

Gene expression databases

BgeeiENSMUSG00000020646.
GenevisibleiQ8R3I2. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000106567.

Structurei

3D structure databases

ProteinModelPortaliQ8R3I2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2704. Eukaryota.
COG5202. LUCA.
GeneTreeiENSGT00550000074565.
HOGENOMiHOG000015994.
HOVERGENiHBG058823.
InParanoidiQ8R3I2.
KOiK13517.
OMAiPVERNIE.
OrthoDBiEOG091G06A8.
PhylomeDBiQ8R3I2.
TreeFamiTF314906.

Family and domain databases

InterProiIPR004299. MBOAT_fam.
[Graphical view]
PfamiPF03062. MBOAT. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R3I2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATTSTTGST LLQPLSNAVQ LPIDQVNFVV CQLFALLAAV WFRTYLHSSK
60 70 80 90 100
TSSFIRHVVA TLLGLYLAFF CFGWYALHFL VQSGISYCIM IIAGVESMQQ
110 120 130 140 150
CCFVFALGYL SVCQITRVYI FDYGQYSADF SGPMMIITQK ITSLAYEIHD
160 170 180 190 200
GMFRKDEELT PSQRGLAVRR MPSLLEYVSY TCNFMGILAG PLCSYKDYIA
210 220 230 240 250
FIEGRASHVA QPSENGKDEQ HGKADPSPNA AVTEKLLVCG LSLLFHLTIS
260 270 280 290 300
NMLPVEYNID EHFQATASWP TKATYLYVSL LAARPKYYFA WTLADAINNA
310 320 330 340 350
AGFGFRGYDK NGVARWDLIS NLRIQQIEMS TSFKMFLDNW NIQTALWLKR
360 370 380 390 400
VCYERATFSP TIQTFFLSAI WHGVYPGYYL TFLTGVLMTL AARAVRNNFR
410 420 430 440 450
HYFLEPPQLK LFYDLITWVA TQITISYTVV PFVLLSIKPS FTFYSSWYYC
460 470 480 490 500
LHVCSILVLL LLPVKKSQRR TSTQENVHLS QAKKFDERDN PLGQNSFSTM
510
NNVCNQNRDT GSRHSSLTQ
Length:519
Mass (Da):58,995
Last modified:June 1, 2002 - v1
Checksum:iA21F3947612314EE
GO
Isoform 2 (identifier: Q8R3I2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     100-132: QCCFVFALGYLSVCQITRVYIFDYGQYSADFSG → H

Show »
Length:487
Mass (Da):55,385
Checksum:i351F46E21169C1A6
GO
Isoform 3 (identifier: Q8R3I2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-141: Missing.
     142-150: TSLAYEIHD → MDNILLIFQ

Note: No experimental confirmation available.
Show »
Length:378
Mass (Da):43,297
Checksum:i0B9F5DCABA3A4454
GO

Sequence cautioni

The sequence BAC27567 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28F → S in BAB28556 (PubMed:16141072).Curated1
Sequence conflicti40V → L in BAB28556 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0224571 – 141Missing in isoform 3. 1 PublicationAdd BLAST141
Alternative sequenceiVSP_022458100 – 132QCCFV…ADFSG → H in isoform 2. 2 PublicationsAdd BLAST33
Alternative sequenceiVSP_022459142 – 150TSLAYEIHD → MDNILLIFQ in isoform 3. 1 Publication9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB305046 mRNA. Translation: BAF93902.1.
AB297383 mRNA. Translation: BAG12122.1.
AK012931 mRNA. Translation: BAB28556.1.
AK031824 mRNA. Translation: BAC27567.1. Different initiation.
AK033106 mRNA. Translation: BAC28154.1.
AK034873 mRNA. Translation: BAC28863.1.
AK076045 mRNA. Translation: BAC36144.1.
BC025020 mRNA. Translation: AAH25020.1.
CCDSiCCDS25845.1. [Q8R3I2-1]
CCDS36423.1. [Q8R3I2-2]
RefSeqiNP_001076810.1. NM_001083341.1. [Q8R3I2-2]
NP_080313.2. NM_026037.3. [Q8R3I2-1]
XP_006515232.1. XM_006515169.2. [Q8R3I2-3]
XP_006515233.1. XM_006515170.3. [Q8R3I2-3]
UniGeneiMm.167671.

Genome annotation databases

EnsembliENSMUST00000078902; ENSMUSP00000077937; ENSMUSG00000020646. [Q8R3I2-2]
ENSMUST00000110942; ENSMUSP00000106567; ENSMUSG00000020646. [Q8R3I2-1]
GeneIDi67216.
KEGGimmu:67216.
UCSCiuc007neu.1. mouse. [Q8R3I2-1]
uc007nev.1. mouse. [Q8R3I2-2]
uc007nex.1. mouse. [Q8R3I2-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB305046 mRNA. Translation: BAF93902.1.
AB297383 mRNA. Translation: BAG12122.1.
AK012931 mRNA. Translation: BAB28556.1.
AK031824 mRNA. Translation: BAC27567.1. Different initiation.
AK033106 mRNA. Translation: BAC28154.1.
AK034873 mRNA. Translation: BAC28863.1.
AK076045 mRNA. Translation: BAC36144.1.
BC025020 mRNA. Translation: AAH25020.1.
CCDSiCCDS25845.1. [Q8R3I2-1]
CCDS36423.1. [Q8R3I2-2]
RefSeqiNP_001076810.1. NM_001083341.1. [Q8R3I2-2]
NP_080313.2. NM_026037.3. [Q8R3I2-1]
XP_006515232.1. XM_006515169.2. [Q8R3I2-3]
XP_006515233.1. XM_006515170.3. [Q8R3I2-3]
UniGeneiMm.167671.

3D structure databases

ProteinModelPortaliQ8R3I2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000106567.

Chemistry databases

ChEMBLiCHEMBL1255156.
SwissLipidsiSLP:000000287.

PTM databases

iPTMnetiQ8R3I2.
PhosphoSitePlusiQ8R3I2.

Proteomic databases

PaxDbiQ8R3I2.
PeptideAtlasiQ8R3I2.
PRIDEiQ8R3I2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000078902; ENSMUSP00000077937; ENSMUSG00000020646. [Q8R3I2-2]
ENSMUST00000110942; ENSMUSP00000106567; ENSMUSG00000020646. [Q8R3I2-1]
GeneIDi67216.
KEGGimmu:67216.
UCSCiuc007neu.1. mouse. [Q8R3I2-1]
uc007nev.1. mouse. [Q8R3I2-2]
uc007nex.1. mouse. [Q8R3I2-3]

Organism-specific databases

CTDi129642.
MGIiMGI:1914466. Mboat2.

Phylogenomic databases

eggNOGiKOG2704. Eukaryota.
COG5202. LUCA.
GeneTreeiENSGT00550000074565.
HOGENOMiHOG000015994.
HOVERGENiHBG058823.
InParanoidiQ8R3I2.
KOiK13517.
OMAiPVERNIE.
OrthoDBiEOG091G06A8.
PhylomeDBiQ8R3I2.
TreeFamiTF314906.

Enzyme and pathway databases

UniPathwayiUPA00085.
BRENDAi2.3.1.23. 3474.
ReactomeiR-MMU-1482788. Acyl chain remodelling of PC.
R-MMU-1482839. Acyl chain remodelling of PE.

Miscellaneous databases

PROiQ8R3I2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020646.
GenevisibleiQ8R3I2. MM.

Family and domain databases

InterProiIPR004299. MBOAT_fam.
[Graphical view]
PfamiPF03062. MBOAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMBOA2_MOUSE
AccessioniPrimary (citable) accession number: Q8R3I2
Secondary accession number(s): A9EDS7
, Q8BHH5, Q8BM56, Q8R192, Q9CZ73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: June 1, 2002
Last modified: November 30, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.