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Q8R3F9

- STPAP_MOUSE

UniProt

Q8R3F9 - STPAP_MOUSE

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Protein

Speckle targeted PIP5K1A-regulated poly(A) polymerase

Gene

Tut1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation (By similarity).By similarity

Catalytic activityi

UTP + RNA(n) = diphosphate + RNA(n+1).
ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Magnesium or manganese.By similarity

Enzyme regulationi

Adenylyltransferase activity is specifically phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi216 – 2161Magnesium or manganese; catalyticBy similarity
Metal bindingi218 – 2181Magnesium or manganese; catalyticBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri16 – 4025C2H2-typeAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. mRNA 3'-UTR binding Source: UniProtKB
  4. polynucleotide adenylyltransferase activity Source: UniProtKB
  5. RNA binding Source: UniProtKB
  6. RNA uridylyltransferase activity Source: UniProtKB

GO - Biological processi

  1. mRNA cleavage Source: UniProtKB
  2. mRNA polyadenylation Source: UniProtKB
  3. snRNA processing Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Speckle targeted PIP5K1A-regulated poly(A) polymerase (EC:2.7.7.19)
Short name:
Star-PAP
Alternative name(s):
RNA-binding motif protein 21
Short name:
RNA-binding protein 21
U6 snRNA-specific terminal uridylyltransferase 1 (EC:2.7.7.52)
Short name:
U6-TUTase
Gene namesi
Name:Tut1
Synonyms:Rbm21
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:1917294. Tut1.

Subcellular locationi

Nucleusnucleolus By similarity. Nucleus speckle By similarity

GO - Cellular componenti

  1. nuclear speck Source: UniProtKB
  2. nucleolus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 869869Speckle targeted PIP5K1A-regulated poly(A) polymerasePRO_0000254187Add
BLAST

Post-translational modificationi

Phosphorylated by CK1 in the proline-rich (Pro-rich) region.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8R3F9.
PaxDbiQ8R3F9.
PRIDEiQ8R3F9.

PTM databases

PhosphoSiteiQ8R3F9.

Expressioni

Gene expression databases

BgeeiQ8R3F9.
GenevestigatoriQ8R3F9.

Interactioni

Subunit structurei

Associates with the cleavage and polyadenylation specificity factor (CPSF) complex. Interacts with CPSF1 and CPSF3; the interaction is direct. Interacts with PIP5K1A; interaction (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ8R3F9.
SMRiQ8R3F9. Positions 55-141, 326-582.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini56 – 12873RRMPROSITE-ProRule annotationAdd
BLAST
Domaini494 – 55259PAP-associatedAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi229 – 31284Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the DNA polymerase type-B-like family.Curated
Contains 1 C2H2-type zinc finger.Curated
Contains 1 PAP-associated domain.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri16 – 4025C2H2-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5260.
GeneTreeiENSGT00550000074490.
HOVERGENiHBG079670.
InParanoidiQ8R3F9.
OMAiREALGCH.
OrthoDBiEOG7353WD.
PhylomeDBiQ8R3F9.
TreeFamiTF354308.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR002058. PAP_assoc.
IPR000504. RRM_dom.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF03828. PAP_assoc. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8R3F9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAVDSDVVS LPRGRFRCCL CDVTTANRPS LDAHLKGRKH RDLVQLRATR
60 70 80 90 100
KAQGLRSVFV SGFPRDVGSA QLSEYFQTFG PVANIVMDKD KGVFAIVEMG
110 120 130 140 150
DISAREAVLS QPKHSLGGHG LRVRPREQKE FQSPASKSPK GVDSSSHQLV
160 170 180 190 200
QALAEAADVG AQMVKLVELR ELSEAERQLR NLVVALMQEV FTEFFPGCVV
210 220 230 240 250
HPFGSTVNSF DVHGCDLDLF LDMGDMEETE PDPKAPKVPE TSSLDSALAS
260 270 280 290 300
SLDPQALACT PASPLDSLSP TSVQESESLD FDTPSSLAPQ TPDSALGSDT
310 320 330 340 350
VTSPQSLPPV SPLQEDRKEG KQGKELELAE EASKDEKEEA AAVLELVGSI
360 370 380 390 400
LRGCVPGVYR VQTVPSARRP VVKFCHRPSG LHGDVSLSNR LALYNSRFLN
410 420 430 440 450
LCSEMDGRVR PLVYTLRCWA QHNGLSGGGP LLNNYALTLL VIYFLQTRDP
460 470 480 490 500
PVLPTVAQLT QRAGEGEQVE VDGWDCSFPK DASRLEPSTN VEPLSSLLAQ
510 520 530 540 550
FFSCVSCLDL SGSLLSLREG RPLMVAEGLP SDLWEGLRLG PMNLQDPFDL
560 570 580 590 600
SHNVAANVTG RVAKRLQSCC GAAASYCRSL QYQQRSSRGR DWGLLPLLQP
610 620 630 640 650
SSPSSLLSAK LIPLPSAPFP QVIMALVDVL REALGCHIEQ GTKRRRSEGA
660 670 680 690 700
RIKDSPLGGV NKRQRLGGQE KSFEEGKEEP QGCAGDHSEN EVEEMVIEVR
710 720 730 740 750
ETPQDWALLH SGPPEEELPL MTANCLDKAA EHNPMKPEVA GEGSQGETGK
760 770 780 790 800
EASHPSSVSW RCALWHQVWQ GRRRARRRLQ QQTKEEGRGG PTTGAEWLAM
810 820 830 840 850
EARVTQELKG PNSEQERPPG EPLLSFVASA SQAEQTLTVA PLQDSQGLFP
860
GLHHFLQGFI PQALKNLLK
Length:869
Mass (Da):94,603
Last modified:June 1, 2002 - v1
Checksum:i0D0D2C73AAC78A25
GO
Isoform 2 (identifier: Q8R3F9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     391-405: LALYNSRFLNLCSEM → YFCVGLKAGSKVWGI
     406-869: Missing.

Note: No experimental confirmation available.

Show »
Length:405
Mass (Da):43,617
Checksum:iDE8F28DDC4C52B65
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei391 – 40515LALYN…LCSEM → YFCVGLKAGSKVWGI in isoform 2. 1 PublicationVSP_021201Add
BLAST
Alternative sequencei406 – 869464Missing in isoform 2. 1 PublicationVSP_021202Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK138418 mRNA. Translation: BAE23652.1.
BC023900 mRNA. Translation: AAH23900.1.
BC025499 mRNA. Translation: AAH25499.1.
CCDSiCCDS29562.1. [Q8R3F9-1]
RefSeqiNP_932110.1. NM_197993.2. [Q8R3F9-1]
UniGeneiMm.165979.

Genome annotation databases

EnsembliENSMUST00000096239; ENSMUSP00000093958; ENSMUSG00000071645. [Q8R3F9-1]
GeneIDi70044.
KEGGimmu:70044.
UCSCiuc008gog.1. mouse. [Q8R3F9-2]
uc008goh.1. mouse. [Q8R3F9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK138418 mRNA. Translation: BAE23652.1 .
BC023900 mRNA. Translation: AAH23900.1 .
BC025499 mRNA. Translation: AAH25499.1 .
CCDSi CCDS29562.1. [Q8R3F9-1 ]
RefSeqi NP_932110.1. NM_197993.2. [Q8R3F9-1 ]
UniGenei Mm.165979.

3D structure databases

ProteinModelPortali Q8R3F9.
SMRi Q8R3F9. Positions 55-141, 326-582.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q8R3F9.

Proteomic databases

MaxQBi Q8R3F9.
PaxDbi Q8R3F9.
PRIDEi Q8R3F9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000096239 ; ENSMUSP00000093958 ; ENSMUSG00000071645 . [Q8R3F9-1 ]
GeneIDi 70044.
KEGGi mmu:70044.
UCSCi uc008gog.1. mouse. [Q8R3F9-2 ]
uc008goh.1. mouse. [Q8R3F9-1 ]

Organism-specific databases

CTDi 64852.
MGIi MGI:1917294. Tut1.

Phylogenomic databases

eggNOGi COG5260.
GeneTreei ENSGT00550000074490.
HOVERGENi HBG079670.
InParanoidi Q8R3F9.
OMAi REALGCH.
OrthoDBi EOG7353WD.
PhylomeDBi Q8R3F9.
TreeFami TF354308.

Miscellaneous databases

NextBioi 330881.
PROi Q8R3F9.
SOURCEi Search...

Gene expression databases

Bgeei Q8R3F9.
Genevestigatori Q8R3F9.

Family and domain databases

Gene3Di 3.30.70.330. 1 hit.
InterProi IPR012677. Nucleotide-bd_a/b_plait.
IPR002058. PAP_assoc.
IPR000504. RRM_dom.
IPR015880. Znf_C2H2-like.
[Graphical view ]
Pfami PF03828. PAP_assoc. 1 hit.
[Graphical view ]
SMARTi SM00360. RRM. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view ]
PROSITEi PS50102. RRM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Hypothalamus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiSTPAP_MOUSE
AccessioniPrimary (citable) accession number: Q8R3F9
Secondary accession number(s): Q3UUH3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: June 1, 2002
Last modified: October 29, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3