Reviewed, UniProtKB/Swiss-Prot Q8R3F9 (TUT1_MOUSE)

Names and origin

Protein names

Recommended name:
    U6 snRNA-specific terminal uridylyltransferase 1
      Short name=U6-TUTase
    EC=2.7.7.52
Alternative name(s):
    RNA-binding motif protein 21
      Short name=RNA-binding protein 21

Gene names

Name:	Tut1
Synonyms:	Rbm21

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	869 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Highly specific terminal uridylyltransferase that exclusively accepts U6 snRNA as substrate. U6 snRNA is unique in that nucleotides are both added to and removed from its 3'-end. U6-TUTase is responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA By similarity.
Catalytic activity	UTP + RNA(n) = diphosphate + RNA(n+1).
Subcellular location	Nucleus › nucleolus By similarity.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.3
Sequence similarities	Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	RNA-binding
Molecular function	Nucleotidyltransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA uridylyltransferase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8R3F9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8R3F9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     391-405: LALYNSRFLNLCSEM → YFCVGLKAGSKVWGI
     406-869: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 869	869	U6 snRNA-specific terminal uridylyltransferase 1		PRO_0000254187
Regions
	Domain	56 – 128	73	RRM
Amino acid modifications
	Modified residue	744	1	Phosphoserine Ref.3
Natural variations
	Alternative sequence	391 – 405	15	LALYN…LCSEM → YFCVGLKAGSKVWGI in isoform 2.		VSP_021201
	Alternative sequence	406 – 869	464	Missing in isoform 2.		VSP_021202

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 0D0D2C73AAC78A25	FASTA	869	94,603
	10 20 30 40 50 60 MAAVDSDVVS LPRGRFRCCL CDVTTANRPS LDAHLKGRKH RDLVQLRATR KAQGLRSVFV 70 80 90 100 110 120 SGFPRDVGSA QLSEYFQTFG PVANIVMDKD KGVFAIVEMG DISAREAVLS QPKHSLGGHG 130 140 150 160 170 180 LRVRPREQKE FQSPASKSPK GVDSSSHQLV QALAEAADVG AQMVKLVELR ELSEAERQLR 190 200 210 220 230 240 NLVVALMQEV FTEFFPGCVV HPFGSTVNSF DVHGCDLDLF LDMGDMEETE PDPKAPKVPE 250 260 270 280 290 300 TSSLDSALAS SLDPQALACT PASPLDSLSP TSVQESESLD FDTPSSLAPQ TPDSALGSDT 310 320 330 340 350 360 VTSPQSLPPV SPLQEDRKEG KQGKELELAE EASKDEKEEA AAVLELVGSI LRGCVPGVYR 370 380 390 400 410 420 VQTVPSARRP VVKFCHRPSG LHGDVSLSNR LALYNSRFLN LCSEMDGRVR PLVYTLRCWA 430 440 450 460 470 480 QHNGLSGGGP LLNNYALTLL VIYFLQTRDP PVLPTVAQLT QRAGEGEQVE VDGWDCSFPK 490 500 510 520 530 540 DASRLEPSTN VEPLSSLLAQ FFSCVSCLDL SGSLLSLREG RPLMVAEGLP SDLWEGLRLG 550 560 570 580 590 600 PMNLQDPFDL SHNVAANVTG RVAKRLQSCC GAAASYCRSL QYQQRSSRGR DWGLLPLLQP 610 620 630 640 650 660 SSPSSLLSAK LIPLPSAPFP QVIMALVDVL REALGCHIEQ GTKRRRSEGA RIKDSPLGGV 670 680 690 700 710 720 NKRQRLGGQE KSFEEGKEEP QGCAGDHSEN EVEEMVIEVR ETPQDWALLH SGPPEEELPL 730 740 750 760 770 780 MTANCLDKAA EHNPMKPEVA GEGSQGETGK EASHPSSVSW RCALWHQVWQ GRRRARRRLQ 790 800 810 820 830 840 QQTKEEGRGG PTTGAEWLAM EARVTQELKG PNSEQERPPG EPLLSFVASA SQAEQTLTVA 850 860 PLQDSQGLFP GLHHFLQGFI PQALKNLLK
	Isoform 2. Checksum: DE8F28DDC4C52B65	FASTA	405	43,617
	10 20 30 40 50 60 MAAVDSDVVS LPRGRFRCCL CDVTTANRPS LDAHLKGRKH RDLVQLRATR KAQGLRSVFV 70 80 90 100 110 120 SGFPRDVGSA QLSEYFQTFG PVANIVMDKD KGVFAIVEMG DISAREAVLS QPKHSLGGHG 130 140 150 160 170 180 LRVRPREQKE FQSPASKSPK GVDSSSHQLV QALAEAADVG AQMVKLVELR ELSEAERQLR 190 200 210 220 230 240 NLVVALMQEV FTEFFPGCVV HPFGSTVNSF DVHGCDLDLF LDMGDMEETE PDPKAPKVPE 250 260 270 280 290 300 TSSLDSALAS SLDPQALACT PASPLDSLSP TSVQESESLD FDTPSSLAPQ TPDSALGSDT 310 320 330 340 350 360 VTSPQSLPPV SPLQEDRKEG KQGKELELAE EASKDEKEEA AAVLELVGSI LRGCVPGVYR 370 380 390 400 VQTVPSARRP VVKFCHRPSG LHGDVSLSNR YFCVGLKAGS KVWGI

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Hypothalamus.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Mammary tumor.
[3]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-744, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK138418 mRNA. Translation: BAE23652.1. BC023900 mRNA. Translation: AAH23900.1. BC025499 mRNA. Translation: AAH25499.1.
IPI	IPI00153749. IPI00798562.
RefSeq	NP_932110.1.
UniGene	Mm.165979
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q8R3F9.
Proteomic databases
PRIDE	Q8R3F9.
Genome annotation databases
Ensembl	ENSMUSG00000071645. Mus musculus. [Contig view]
GeneID	70044.
KEGG	mmu:70044.
Organism-specific databases
MGI	MGI:1917294. Tut1.
Phylogenomic databases
HOVERGEN	Q8R3F9.
OMA	Q8R3F9. QGGTSKR.
Enzyme and pathway databases
BRENDA	2.7.7.52. 244.
Gene expression databases
ArrayExpress	Q8R3F9.
Bgee	Q8R3F9.
GermOnline	ENSMUSG00000071645. Mus musculus.
Family and domain databases
InterPro	IPR012677. a_b_plait_nuc_bd. IPR002058. PAP_assoc. IPR000504. RRM_RNP1. IPR015880. Znf_C2H2-like. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit.
Pfam	PF03828. PAP_assoc. 1 hit. PF00076. RRM_1. 1 hit. [Graphical view]
SMART	SM00360. RRM. 1 hit. SM00355. ZnF_C2H2. 1 hit. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	330881.
SOURCE	Search...

Entry information

Entry name

TUT1_MOUSE

Accession

Primary (citable) accession number: Q8R3F9
Secondary accession number(s): Q3UUH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)