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Q8R3F9

- STPAP_MOUSE

UniProt

Q8R3F9 - STPAP_MOUSE

Protein

Speckle targeted PIP5K1A-regulated poly(A) polymerase

Gene

Tut1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation By similarity.By similarity

    Catalytic activityi

    UTP + RNA(n) = diphosphate + RNA(n+1).
    ATP + RNA(n) = diphosphate + RNA(n+1).

    Cofactori

    Magnesium or manganese.By similarity

    Enzyme regulationi

    Adenylyltransferase activity is specifically phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2).By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi216 – 2161Magnesium or manganese; catalyticBy similarity
    Metal bindingi218 – 2181Magnesium or manganese; catalyticBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri16 – 4025C2H2-typeAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. mRNA 3'-UTR binding Source: UniProtKB
    4. polynucleotide adenylyltransferase activity Source: UniProtKB
    5. RNA binding Source: UniProtKB
    6. RNA uridylyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. mRNA cleavage Source: UniProtKB
    2. mRNA polyadenylation Source: UniProtKB
    3. snRNA processing Source: UniProtKB

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    mRNA processing

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Speckle targeted PIP5K1A-regulated poly(A) polymerase (EC:2.7.7.19)
    Short name:
    Star-PAP
    Alternative name(s):
    RNA-binding motif protein 21
    Short name:
    RNA-binding protein 21
    U6 snRNA-specific terminal uridylyltransferase 1 (EC:2.7.7.52)
    Short name:
    U6-TUTase
    Gene namesi
    Name:Tut1
    Synonyms:Rbm21
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:1917294. Tut1.

    Subcellular locationi

    Nucleusnucleolus By similarity. Nucleus speckle By similarity

    GO - Cellular componenti

    1. nuclear speck Source: UniProtKB
    2. nucleolus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 869869Speckle targeted PIP5K1A-regulated poly(A) polymerasePRO_0000254187Add
    BLAST

    Post-translational modificationi

    Phosphorylated by CK1 in the proline-rich (Pro-rich) region.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8R3F9.
    PaxDbiQ8R3F9.
    PRIDEiQ8R3F9.

    PTM databases

    PhosphoSiteiQ8R3F9.

    Expressioni

    Gene expression databases

    BgeeiQ8R3F9.
    GenevestigatoriQ8R3F9.

    Interactioni

    Subunit structurei

    Associates with the cleavage and polyadenylation specificity factor (CPSF) complex. Interacts with CPSF1 and CPSF3; the interaction is direct. Interacts with PIP5K1A; interaction By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ8R3F9.
    SMRiQ8R3F9. Positions 23-141, 326-582.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini56 – 12873RRMPROSITE-ProRule annotationAdd
    BLAST
    Domaini494 – 55259PAP-associatedAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi229 – 31284Pro-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the DNA polymerase type-B-like family.Curated
    Contains 1 C2H2-type zinc finger.Curated
    Contains 1 PAP-associated domain.Curated
    Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri16 – 4025C2H2-typeAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5260.
    GeneTreeiENSGT00550000074490.
    HOVERGENiHBG079670.
    InParanoidiQ8R3F9.
    OMAiREALGCH.
    OrthoDBiEOG7353WD.
    PhylomeDBiQ8R3F9.
    TreeFamiTF354308.

    Family and domain databases

    Gene3Di3.30.70.330. 1 hit.
    InterProiIPR012677. Nucleotide-bd_a/b_plait.
    IPR002058. PAP_assoc.
    IPR000504. RRM_dom.
    IPR015880. Znf_C2H2-like.
    [Graphical view]
    PfamiPF03828. PAP_assoc. 1 hit.
    [Graphical view]
    SMARTiSM00360. RRM. 1 hit.
    SM00355. ZnF_C2H2. 1 hit.
    [Graphical view]
    PROSITEiPS50102. RRM. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8R3F9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAVDSDVVS LPRGRFRCCL CDVTTANRPS LDAHLKGRKH RDLVQLRATR    50
    KAQGLRSVFV SGFPRDVGSA QLSEYFQTFG PVANIVMDKD KGVFAIVEMG 100
    DISAREAVLS QPKHSLGGHG LRVRPREQKE FQSPASKSPK GVDSSSHQLV 150
    QALAEAADVG AQMVKLVELR ELSEAERQLR NLVVALMQEV FTEFFPGCVV 200
    HPFGSTVNSF DVHGCDLDLF LDMGDMEETE PDPKAPKVPE TSSLDSALAS 250
    SLDPQALACT PASPLDSLSP TSVQESESLD FDTPSSLAPQ TPDSALGSDT 300
    VTSPQSLPPV SPLQEDRKEG KQGKELELAE EASKDEKEEA AAVLELVGSI 350
    LRGCVPGVYR VQTVPSARRP VVKFCHRPSG LHGDVSLSNR LALYNSRFLN 400
    LCSEMDGRVR PLVYTLRCWA QHNGLSGGGP LLNNYALTLL VIYFLQTRDP 450
    PVLPTVAQLT QRAGEGEQVE VDGWDCSFPK DASRLEPSTN VEPLSSLLAQ 500
    FFSCVSCLDL SGSLLSLREG RPLMVAEGLP SDLWEGLRLG PMNLQDPFDL 550
    SHNVAANVTG RVAKRLQSCC GAAASYCRSL QYQQRSSRGR DWGLLPLLQP 600
    SSPSSLLSAK LIPLPSAPFP QVIMALVDVL REALGCHIEQ GTKRRRSEGA 650
    RIKDSPLGGV NKRQRLGGQE KSFEEGKEEP QGCAGDHSEN EVEEMVIEVR 700
    ETPQDWALLH SGPPEEELPL MTANCLDKAA EHNPMKPEVA GEGSQGETGK 750
    EASHPSSVSW RCALWHQVWQ GRRRARRRLQ QQTKEEGRGG PTTGAEWLAM 800
    EARVTQELKG PNSEQERPPG EPLLSFVASA SQAEQTLTVA PLQDSQGLFP 850
    GLHHFLQGFI PQALKNLLK 869
    Length:869
    Mass (Da):94,603
    Last modified:June 1, 2002 - v1
    Checksum:i0D0D2C73AAC78A25
    GO
    Isoform 2 (identifier: Q8R3F9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         391-405: LALYNSRFLNLCSEM → YFCVGLKAGSKVWGI
         406-869: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:405
    Mass (Da):43,617
    Checksum:iDE8F28DDC4C52B65
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei391 – 40515LALYN…LCSEM → YFCVGLKAGSKVWGI in isoform 2. 1 PublicationVSP_021201Add
    BLAST
    Alternative sequencei406 – 869464Missing in isoform 2. 1 PublicationVSP_021202Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK138418 mRNA. Translation: BAE23652.1.
    BC023900 mRNA. Translation: AAH23900.1.
    BC025499 mRNA. Translation: AAH25499.1.
    CCDSiCCDS29562.1. [Q8R3F9-1]
    RefSeqiNP_932110.1. NM_197993.2. [Q8R3F9-1]
    UniGeneiMm.165979.

    Genome annotation databases

    EnsembliENSMUST00000096239; ENSMUSP00000093958; ENSMUSG00000071645. [Q8R3F9-1]
    GeneIDi70044.
    KEGGimmu:70044.
    UCSCiuc008gog.1. mouse. [Q8R3F9-2]
    uc008goh.1. mouse. [Q8R3F9-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK138418 mRNA. Translation: BAE23652.1 .
    BC023900 mRNA. Translation: AAH23900.1 .
    BC025499 mRNA. Translation: AAH25499.1 .
    CCDSi CCDS29562.1. [Q8R3F9-1 ]
    RefSeqi NP_932110.1. NM_197993.2. [Q8R3F9-1 ]
    UniGenei Mm.165979.

    3D structure databases

    ProteinModelPortali Q8R3F9.
    SMRi Q8R3F9. Positions 23-141, 326-582.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8R3F9.

    Proteomic databases

    MaxQBi Q8R3F9.
    PaxDbi Q8R3F9.
    PRIDEi Q8R3F9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000096239 ; ENSMUSP00000093958 ; ENSMUSG00000071645 . [Q8R3F9-1 ]
    GeneIDi 70044.
    KEGGi mmu:70044.
    UCSCi uc008gog.1. mouse. [Q8R3F9-2 ]
    uc008goh.1. mouse. [Q8R3F9-1 ]

    Organism-specific databases

    CTDi 64852.
    MGIi MGI:1917294. Tut1.

    Phylogenomic databases

    eggNOGi COG5260.
    GeneTreei ENSGT00550000074490.
    HOVERGENi HBG079670.
    InParanoidi Q8R3F9.
    OMAi REALGCH.
    OrthoDBi EOG7353WD.
    PhylomeDBi Q8R3F9.
    TreeFami TF354308.

    Miscellaneous databases

    NextBioi 330881.
    PROi Q8R3F9.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8R3F9.
    Genevestigatori Q8R3F9.

    Family and domain databases

    Gene3Di 3.30.70.330. 1 hit.
    InterProi IPR012677. Nucleotide-bd_a/b_plait.
    IPR002058. PAP_assoc.
    IPR000504. RRM_dom.
    IPR015880. Znf_C2H2-like.
    [Graphical view ]
    Pfami PF03828. PAP_assoc. 1 hit.
    [Graphical view ]
    SMARTi SM00360. RRM. 1 hit.
    SM00355. ZnF_C2H2. 1 hit.
    [Graphical view ]
    PROSITEi PS50102. RRM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Hypothalamus.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Mammary tumor.
    3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiSTPAP_MOUSE
    AccessioniPrimary (citable) accession number: Q8R3F9
    Secondary accession number(s): Q3UUH3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3