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Protein

Speckle targeted PIP5K1A-regulated poly(A) polymerase

Gene

Tut1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation (By similarity).By similarity

Catalytic activityi

UTP + RNA(n) = diphosphate + RNA(n+1).
ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarity

Enzyme regulationi

Adenylyltransferase activity is specifically phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi216Magnesium or manganese; catalyticBy similarity1
Metal bindingi218Magnesium or manganese; catalyticBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri16 – 40C2H2-typeAdd BLAST25

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Speckle targeted PIP5K1A-regulated poly(A) polymerase (EC:2.7.7.19)
Short name:
Star-PAP
Alternative name(s):
RNA-binding motif protein 21
Short name:
RNA-binding protein 21
U6 snRNA-specific terminal uridylyltransferase 1 (EC:2.7.7.52)
Short name:
U6-TUTase
Gene namesi
Name:Tut1
Synonyms:Rbm21
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1917294. Tut1.

Subcellular locationi

  • Nucleusnucleolus By similarity
  • Nucleus speckle By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002541871 – 869Speckle targeted PIP5K1A-regulated poly(A) polymeraseAdd BLAST869

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei688PhosphoserineCombined sources1
Modified residuei744PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by CK1 in the proline-rich (Pro-rich) region.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8R3F9.
PaxDbiQ8R3F9.
PeptideAtlasiQ8R3F9.
PRIDEiQ8R3F9.

PTM databases

iPTMnetiQ8R3F9.
PhosphoSitePlusiQ8R3F9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000071645.
GenevisibleiQ8R3F9. MM.

Interactioni

Subunit structurei

Associates with the cleavage and polyadenylation specificity factor (CPSF) complex. Interacts with CPSF1 and CPSF3; the interaction is direct. Interacts with PIP5K1A; interaction (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000093958.

Structurei

3D structure databases

ProteinModelPortaliQ8R3F9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini56 – 128RRMPROSITE-ProRule annotationAdd BLAST73
Domaini494 – 552PAP-associatedAdd BLAST59

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi229 – 312Pro-richAdd BLAST84

Sequence similaritiesi

Belongs to the DNA polymerase type-B-like family.Curated
Contains 1 C2H2-type zinc finger.Curated
Contains 1 PAP-associated domain.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri16 – 40C2H2-typeAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2277. Eukaryota.
COG5260. LUCA.
GeneTreeiENSGT00550000074490.
HOVERGENiHBG079670.
InParanoidiQ8R3F9.
KOiK18709.
OMAiREALGCH.
OrthoDBiEOG091G091J.
PhylomeDBiQ8R3F9.
TreeFamiTF354308.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR002058. PAP_assoc.
IPR000504. RRM_dom.
IPR003604. Znf_U1.
[Graphical view]
PfamiPF03828. PAP_assoc. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00451. ZnF_U1. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R3F9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAVDSDVVS LPRGRFRCCL CDVTTANRPS LDAHLKGRKH RDLVQLRATR
60 70 80 90 100
KAQGLRSVFV SGFPRDVGSA QLSEYFQTFG PVANIVMDKD KGVFAIVEMG
110 120 130 140 150
DISAREAVLS QPKHSLGGHG LRVRPREQKE FQSPASKSPK GVDSSSHQLV
160 170 180 190 200
QALAEAADVG AQMVKLVELR ELSEAERQLR NLVVALMQEV FTEFFPGCVV
210 220 230 240 250
HPFGSTVNSF DVHGCDLDLF LDMGDMEETE PDPKAPKVPE TSSLDSALAS
260 270 280 290 300
SLDPQALACT PASPLDSLSP TSVQESESLD FDTPSSLAPQ TPDSALGSDT
310 320 330 340 350
VTSPQSLPPV SPLQEDRKEG KQGKELELAE EASKDEKEEA AAVLELVGSI
360 370 380 390 400
LRGCVPGVYR VQTVPSARRP VVKFCHRPSG LHGDVSLSNR LALYNSRFLN
410 420 430 440 450
LCSEMDGRVR PLVYTLRCWA QHNGLSGGGP LLNNYALTLL VIYFLQTRDP
460 470 480 490 500
PVLPTVAQLT QRAGEGEQVE VDGWDCSFPK DASRLEPSTN VEPLSSLLAQ
510 520 530 540 550
FFSCVSCLDL SGSLLSLREG RPLMVAEGLP SDLWEGLRLG PMNLQDPFDL
560 570 580 590 600
SHNVAANVTG RVAKRLQSCC GAAASYCRSL QYQQRSSRGR DWGLLPLLQP
610 620 630 640 650
SSPSSLLSAK LIPLPSAPFP QVIMALVDVL REALGCHIEQ GTKRRRSEGA
660 670 680 690 700
RIKDSPLGGV NKRQRLGGQE KSFEEGKEEP QGCAGDHSEN EVEEMVIEVR
710 720 730 740 750
ETPQDWALLH SGPPEEELPL MTANCLDKAA EHNPMKPEVA GEGSQGETGK
760 770 780 790 800
EASHPSSVSW RCALWHQVWQ GRRRARRRLQ QQTKEEGRGG PTTGAEWLAM
810 820 830 840 850
EARVTQELKG PNSEQERPPG EPLLSFVASA SQAEQTLTVA PLQDSQGLFP
860
GLHHFLQGFI PQALKNLLK
Length:869
Mass (Da):94,603
Last modified:June 1, 2002 - v1
Checksum:i0D0D2C73AAC78A25
GO
Isoform 2 (identifier: Q8R3F9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     391-405: LALYNSRFLNLCSEM → YFCVGLKAGSKVWGI
     406-869: Missing.

Note: No experimental confirmation available.
Show »
Length:405
Mass (Da):43,617
Checksum:iDE8F28DDC4C52B65
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_021201391 – 405LALYN…LCSEM → YFCVGLKAGSKVWGI in isoform 2. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_021202406 – 869Missing in isoform 2. 1 PublicationAdd BLAST464

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK138418 mRNA. Translation: BAE23652.1.
BC023900 mRNA. Translation: AAH23900.1.
BC025499 mRNA. Translation: AAH25499.1.
CCDSiCCDS29562.1. [Q8R3F9-1]
RefSeqiNP_932110.1. NM_197993.3. [Q8R3F9-1]
UniGeneiMm.165979.

Genome annotation databases

EnsembliENSMUST00000096239; ENSMUSP00000093958; ENSMUSG00000071645. [Q8R3F9-1]
GeneIDi70044.
KEGGimmu:70044.
UCSCiuc008gog.1. mouse. [Q8R3F9-2]
uc008goh.1. mouse. [Q8R3F9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK138418 mRNA. Translation: BAE23652.1.
BC023900 mRNA. Translation: AAH23900.1.
BC025499 mRNA. Translation: AAH25499.1.
CCDSiCCDS29562.1. [Q8R3F9-1]
RefSeqiNP_932110.1. NM_197993.3. [Q8R3F9-1]
UniGeneiMm.165979.

3D structure databases

ProteinModelPortaliQ8R3F9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000093958.

PTM databases

iPTMnetiQ8R3F9.
PhosphoSitePlusiQ8R3F9.

Proteomic databases

EPDiQ8R3F9.
PaxDbiQ8R3F9.
PeptideAtlasiQ8R3F9.
PRIDEiQ8R3F9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000096239; ENSMUSP00000093958; ENSMUSG00000071645. [Q8R3F9-1]
GeneIDi70044.
KEGGimmu:70044.
UCSCiuc008gog.1. mouse. [Q8R3F9-2]
uc008goh.1. mouse. [Q8R3F9-1]

Organism-specific databases

CTDi64852.
MGIiMGI:1917294. Tut1.

Phylogenomic databases

eggNOGiKOG2277. Eukaryota.
COG5260. LUCA.
GeneTreeiENSGT00550000074490.
HOVERGENiHBG079670.
InParanoidiQ8R3F9.
KOiK18709.
OMAiREALGCH.
OrthoDBiEOG091G091J.
PhylomeDBiQ8R3F9.
TreeFamiTF354308.

Miscellaneous databases

PROiQ8R3F9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000071645.
GenevisibleiQ8R3F9. MM.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR002058. PAP_assoc.
IPR000504. RRM_dom.
IPR003604. Znf_U1.
[Graphical view]
PfamiPF03828. PAP_assoc. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00451. ZnF_U1. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTPAP_MOUSE
AccessioniPrimary (citable) accession number: Q8R3F9
Secondary accession number(s): Q3UUH3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: June 1, 2002
Last modified: November 30, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.