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Protein

Malonyl-CoA-acyl carrier protein transacylase, mitochondrial

Gene

Mcat

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a malonyl moiety from malonyl-CoA to the free thiol group of the phosphopantetheine arm of the mitochondrial ACP protein (NDUFAB1). This suggests the existence of the biosynthesis of fatty acids in mitochondrias (By similarity).By similarity

Catalytic activityi

Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein].

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei151 – 1511By similarity
Active sitei268 – 2681By similarity

GO - Molecular functioni

  1. [acyl-carrier-protein] S-malonyltransferase activity Source: MGI
  2. poly(A) RNA binding Source: MGI
  3. transferase activity Source: MGI

GO - Biological processi

  1. fatty acid biosynthetic process Source: MGI
  2. metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Malonyl-CoA-acyl carrier protein transacylase, mitochondrial (EC:2.3.1.39)
Short name:
MCT
Alternative name(s):
Mitochondrial malonyltransferase
[Acyl-carrier-protein] malonyltransferase
Gene namesi
Name:Mcat
Synonyms:Mt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2388651. Mcat.

Subcellular locationi

Mitochondrion By similarity

GO - Cellular componenti

  1. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 381Malonyl-CoA-acyl carrier protein transacylase, mitochondrialPRO_0000042239
Transit peptidei1 – ?MitochondrionSequence Analysis

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei312 – 3121N6-succinyllysine1 Publication

Proteomic databases

MaxQBiQ8R3F5.
PaxDbiQ8R3F5.
PRIDEiQ8R3F5.

PTM databases

PhosphoSiteiQ8R3F5.

Expressioni

Gene expression databases

BgeeiQ8R3F5.
CleanExiMM_MCAT.
GenevestigatoriQ8R3F5.

Interactioni

Protein-protein interaction databases

IntActiQ8R3F5. 1 interaction.
MINTiMINT-4094897.

Structurei

3D structure databases

ProteinModelPortaliQ8R3F5.
SMRiQ8R3F5. Positions 59-373.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the type II malonyltransferase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0331.
GeneTreeiENSGT00390000013715.
HOGENOMiHOG000036504.
HOVERGENiHBG051540.
InParanoidiQ8R3F5.
KOiK00645.
OMAiNEAYHNK.
OrthoDBiEOG7D85X6.
PhylomeDBiQ8R3F5.
TreeFamiTF313401.

Family and domain databases

Gene3Di3.40.366.10. 2 hits.
InterProiIPR001227. Ac_transferase_dom.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR016036. Malonyl_transacylase_ACP-bd.
[Graphical view]
PfamiPF00698. Acyl_transf_1. 1 hit.
[Graphical view]
SUPFAMiSSF52151. SSF52151. 2 hits.
SSF55048. SSF55048. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R3F5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSARVARAGW AWRSWGRRAA SSLREPPPDA VDVAELLRDS SVAEEGAQEA
60 70 80 90 100
VARRRPPSQC SVLLFPGQGC QAVGMGSGLL HLPRVRQLYE AAHRVLGYDL
110 120 130 140 150
LELCLRGPQE DLDRTVHCQP AVFVASLAAV EKLHHLQPAV IDNCVAAAGF
160 170 180 190 200
SVGEFAALVF AGAMDFSEGL YAVKARAEAM QEASEAVPSG MLSVLGQRQS
210 220 230 240 250
NFSFACLEAQ EHCKSLGIEN PVCQVSNYLF PDCRVISGHL EALQFLRRNS
260 270 280 290 300
AKYHFRRTKM LPVSGGFHTC LMEPAVDPLM KVLGSINIKK PLVAVHSNVS
310 320 330 340 350
GQKYTHPQHI RKLLGQQVVS PVKWEQTMHS IYERKKGMEF PSTYEVGPGQ
360 370 380
QLGSILKCCN RQAWKSYSHV DVMQNIMDPD P
Length:381
Mass (Da):41,928
Last modified:October 11, 2005 - v3
Checksum:i7024C1EBAF95B1EE
GO

Sequence cautioni

The sequence AAH25519.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC099494 mRNA. Translation: AAH99494.1.
BC025519 mRNA. Translation: AAH25519.1. Different initiation.
CCDSiCCDS27704.1.
RefSeqiNP_001025185.1. NM_001030014.2.
UniGeneiMm.37560.

Genome annotation databases

EnsembliENSMUST00000061882; ENSMUSP00000051569; ENSMUSG00000048755.
GeneIDi223722.
KEGGimmu:223722.
UCSCiuc007xbf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC099494 mRNA. Translation: AAH99494.1.
BC025519 mRNA. Translation: AAH25519.1. Different initiation.
CCDSiCCDS27704.1.
RefSeqiNP_001025185.1. NM_001030014.2.
UniGeneiMm.37560.

3D structure databases

ProteinModelPortaliQ8R3F5.
SMRiQ8R3F5. Positions 59-373.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8R3F5. 1 interaction.
MINTiMINT-4094897.

PTM databases

PhosphoSiteiQ8R3F5.

Proteomic databases

MaxQBiQ8R3F5.
PaxDbiQ8R3F5.
PRIDEiQ8R3F5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000061882; ENSMUSP00000051569; ENSMUSG00000048755.
GeneIDi223722.
KEGGimmu:223722.
UCSCiuc007xbf.1. mouse.

Organism-specific databases

CTDi27349.
MGIiMGI:2388651. Mcat.

Phylogenomic databases

eggNOGiCOG0331.
GeneTreeiENSGT00390000013715.
HOGENOMiHOG000036504.
HOVERGENiHBG051540.
InParanoidiQ8R3F5.
KOiK00645.
OMAiNEAYHNK.
OrthoDBiEOG7D85X6.
PhylomeDBiQ8R3F5.
TreeFamiTF313401.

Enzyme and pathway databases

UniPathwayiUPA00094.

Miscellaneous databases

ChiTaRSiMcat. mouse.
NextBioi376844.
PROiQ8R3F5.
SOURCEiSearch...

Gene expression databases

BgeeiQ8R3F5.
CleanExiMM_MCAT.
GenevestigatoriQ8R3F5.

Family and domain databases

Gene3Di3.40.366.10. 2 hits.
InterProiIPR001227. Ac_transferase_dom.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR016036. Malonyl_transacylase_ACP-bd.
[Graphical view]
PfamiPF00698. Acyl_transf_1. 1 hit.
[Graphical view]
SUPFAMiSSF52151. SSF52151. 2 hits.
SSF55048. SSF55048. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thyroid.
  2. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-312, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiFABD_MOUSE
AccessioniPrimary (citable) accession number: Q8R3F5
Secondary accession number(s): Q4FZH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: October 11, 2005
Last modified: February 4, 2015
This is version 97 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.