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Protein

WD repeat domain phosphoinositide-interacting protein 1

Gene

Wipi1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in autophagy and in particular starvation- and calcium-mediated autophagy, as well as in mitophagy. Functions upstream of the ATG12-ATG5-ATG16L1 complex and LC3, and downstream of the ULK1 and PI3-kinase complexes. Involved in xenophagy of Staphylococcus aureus. Invading S.aureus cells become entrapped in autophagosome-like WIPI1 positive vesicles targeted for lysosomal degradation. Plays also a distinct role in controlling the transcription of melanogenic enzymes and melanosome maturation, a process that is distinct from starvation-induced autophagy. May also regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Autophagy

Enzyme and pathway databases

ReactomeiR-MMU-1632852. Macroautophagy.

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat domain phosphoinositide-interacting protein 1
Short name:
WIPI-1
Alternative name(s):
WD40 repeat protein interacting with phosphoinositides of 49 kDa
Short name:
WIPI 49 kDa
Gene namesi
Name:Wipi1
Synonyms:D11Ertd498e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1261864. Wipi1.

Subcellular locationi

  • Golgi apparatustrans-Golgi network By similarity
  • Endosome By similarity
  • Cytoplasmic vesicleclathrin-coated vesicle By similarity
  • Preautophagosomal structure membrane 1 Publication; Peripheral membrane protein 1 Publication
  • Cytoplasmcytoskeleton By similarity

  • Note: Trans elements of the Golgi and peripheral endosomes. Dynamically cycles through these compartments and is susceptible to conditions that modulate membrane flux. Enriched in clathrin-coated vesicles. Upon starvation-induced autophagy, accumulates at subcellular structures in the cytoplasm: enlarged vesicular and lasso-like structures, and large cup-shaped structures predominantly around the nucleus. Recruitment to autophagic membranes is controlled by MTMR14. Labile microtubules specifically recruit markers of autophagosome formation like WIPI1, whereas mature autophagosomes may bind to stable microtubules (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 446446WD repeat domain phosphoinositide-interacting protein 1PRO_0000051438Add
BLAST

Proteomic databases

MaxQBiQ8R3E3.
PaxDbiQ8R3E3.
PRIDEiQ8R3E3.

PTM databases

iPTMnetiQ8R3E3.
PhosphoSiteiQ8R3E3.

Expressioni

Gene expression databases

BgeeiQ8R3E3.
CleanExiMM_WIPI1.
ExpressionAtlasiQ8R3E3. baseline and differential.
GenevisibleiQ8R3E3. MM.

Interactioni

Subunit structurei

Interacts with androgen receptor (AR) and the estrogen receptors ESR1 and ESR2. Binds PtdIns3P and to a lesser extent, PtdIns3,5P2 and PtdIns5P in vitro. Interaction with PtdIns3P is required for recruitment to membranes.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099349.

Structurei

3D structure databases

ProteinModelPortaliQ8R3E3.
SMRiQ8R3E3. Positions 20-261.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati184 – 22441WD 1Add
BLAST
Repeati230 – 26940WD 2Add
BLAST
Repeati312 – 35140WD 3Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi131 – 1366Nuclear receptor interactionBy similarity

Domaini

The N-terminus might form a beta-propeller domain involved in specific binding to phosphatidylinositol 3,5-bisphosphate (PIP2), leading to the association of the protein to the membrane. Association to the membrane can also occur through binding to phosphatidylinositol 3-monophosphate (PI3P).

Sequence similaritiesi

Belongs to the WD repeat SVP1 family.Curated
Contains 3 WD repeats.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2110. Eukaryota.
ENOG410XP6Y. LUCA.
GeneTreeiENSGT00730000110845.
HOGENOMiHOG000217544.
HOVERGENiHBG056639.
InParanoidiQ8R3E3.
KOiK17908.
OMAiSTWTGYM.
OrthoDBiEOG7PS1G4.
PhylomeDBiQ8R3E3.
TreeFamiTF314879.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR032909. WIPI1.
[Graphical view]
PANTHERiPTHR11227:SF23. PTHR11227:SF23. 2 hits.
SMARTiSM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R3E3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAEAADAPP GRVEAALSCF SFNQDCTSLA IGTKAGYKLF SLSSVEQLDQ
60 70 80 90 100
VHGSNEIPDV YIVERLFSSS LVVVVSHTKP RQMNVYHFKK GTEICNYSYS
110 120 130 140 150
SNILSIRLNR QRLLVCLEES IYIHNIKDMK LLKTVLDIPS NPTGLCALSI
160 170 180 190 200
NHSNSYLAYP GSQSTGEIVL YDGNSLKTVC TIAAHEGTLA AITFNSSGSK
210 220 230 240 250
LASASEKGTV IRVFSVPEGQ KLYEFRRGMK RYVTISSLVF SMDSQFLCAS
260 270 280 290 300
SNTETVHIFK MEHLTDSRPE EPSTWSGYMG KMFMAATNYL PAQVSDMMNQ
310 320 330 340 350
DRAFATGRLN FSGQKNICTL STIQKLPRLL VASSDGHLYI YNLDPQDGGE
360 370 380 390 400
CVLIKTHSLL SSGTTEENKE NDLRPSLPPS YAATVARPST SAASTVPGYS
410 420 430 440
EDGGALRGEV IPEHEFATGP VCLDDENEFP PIILCRGSQK GKTKQS
Length:446
Mass (Da):48,758
Last modified:June 1, 2002 - v1
Checksum:i9F7FA1CB802E5344
GO
Isoform 2 (identifier: Q8R3E3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     432-446: IILCRGSQKGKTKQS → VSIRNP

Show »
Length:437
Mass (Da):47,796
Checksum:iA2E0FD91A644018D
GO
Isoform 3 (identifier: Q8R3E3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     267-270: SRPE → RCRT
     271-446: Missing.

Note: No experimental confirmation available.
Show »
Length:270
Mass (Da):29,819
Checksum:i4F3C3E0EF20A2C5B
GO

Sequence cautioni

The sequence AAH24811.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAH24883.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei267 – 2704SRPE → RCRT in isoform 3. 1 PublicationVSP_016967
Alternative sequencei271 – 446176Missing in isoform 3. 1 PublicationVSP_016968Add
BLAST
Alternative sequencei432 – 44615IILCR…KTKQS → VSIRNP in isoform 2. 2 PublicationsVSP_016969Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK031672 mRNA. Translation: BAC27504.1.
AK034907 mRNA. Translation: BAC28878.1.
AK050495 mRNA. Translation: BAE20673.1.
AK159608 mRNA. Translation: BAE35227.1.
AK170563 mRNA. Translation: BAE41884.1.
BC024811 mRNA. Translation: AAH24811.1. Different initiation.
BC024883 mRNA. Translation: AAH24883.1. Different initiation.
BC025560 mRNA. Translation: AAH25560.1.
CCDSiCCDS25582.1. [Q8R3E3-1]
RefSeqiNP_666052.1. NM_145940.2. [Q8R3E3-1]
UniGeneiMm.35817.

Genome annotation databases

EnsembliENSMUST00000047186; ENSMUSP00000038635; ENSMUSG00000041895. [Q8R3E3-2]
ENSMUST00000103060; ENSMUSP00000099349; ENSMUSG00000041895. [Q8R3E3-1]
GeneIDi52639.
KEGGimmu:52639.
UCSCiuc007mcq.1. mouse. [Q8R3E3-1]
uc007mcr.1. mouse. [Q8R3E3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK031672 mRNA. Translation: BAC27504.1.
AK034907 mRNA. Translation: BAC28878.1.
AK050495 mRNA. Translation: BAE20673.1.
AK159608 mRNA. Translation: BAE35227.1.
AK170563 mRNA. Translation: BAE41884.1.
BC024811 mRNA. Translation: AAH24811.1. Different initiation.
BC024883 mRNA. Translation: AAH24883.1. Different initiation.
BC025560 mRNA. Translation: AAH25560.1.
CCDSiCCDS25582.1. [Q8R3E3-1]
RefSeqiNP_666052.1. NM_145940.2. [Q8R3E3-1]
UniGeneiMm.35817.

3D structure databases

ProteinModelPortaliQ8R3E3.
SMRiQ8R3E3. Positions 20-261.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099349.

PTM databases

iPTMnetiQ8R3E3.
PhosphoSiteiQ8R3E3.

Proteomic databases

MaxQBiQ8R3E3.
PaxDbiQ8R3E3.
PRIDEiQ8R3E3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047186; ENSMUSP00000038635; ENSMUSG00000041895. [Q8R3E3-2]
ENSMUST00000103060; ENSMUSP00000099349; ENSMUSG00000041895. [Q8R3E3-1]
GeneIDi52639.
KEGGimmu:52639.
UCSCiuc007mcq.1. mouse. [Q8R3E3-1]
uc007mcr.1. mouse. [Q8R3E3-2]

Organism-specific databases

CTDi55062.
MGIiMGI:1261864. Wipi1.

Phylogenomic databases

eggNOGiKOG2110. Eukaryota.
ENOG410XP6Y. LUCA.
GeneTreeiENSGT00730000110845.
HOGENOMiHOG000217544.
HOVERGENiHBG056639.
InParanoidiQ8R3E3.
KOiK17908.
OMAiSTWTGYM.
OrthoDBiEOG7PS1G4.
PhylomeDBiQ8R3E3.
TreeFamiTF314879.

Enzyme and pathway databases

ReactomeiR-MMU-1632852. Macroautophagy.

Miscellaneous databases

ChiTaRSiWipi1. mouse.
NextBioi309255.
PROiQ8R3E3.
SOURCEiSearch...

Gene expression databases

BgeeiQ8R3E3.
CleanExiMM_WIPI1.
ExpressionAtlasiQ8R3E3. baseline and differential.
GenevisibleiQ8R3E3. MM.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR032909. WIPI1.
[Graphical view]
PANTHERiPTHR11227:SF23. PTHR11227:SF23. 2 hits.
SMARTiSM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and NOD.
    Tissue: Dendritic cell, Embryo, Osteoclast, Pancreas and Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 316-325, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Heart.
  5. "Ca2+/calmodulin-dependent kinase (CaMK) signaling via CaMKI and AMP-activated protein kinase contributes to the regulation of WIPI-1 at the onset of autophagy."
    Pfisterer S.G., Mauthe M., Codogno P., Proikas-Cezanne T.
    Mol. Pharmacol. 80:1066-1075(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: REGULATION BY CALCIUM-SIGNALING, SUBCELLULAR LOCATION.
  6. "Structures containing Atg9A and the ULK1 complex independently target depolarized mitochondria at initial stages of Parkin-mediated mitophagy."
    Itakura E., Kishi-Itakura C., Koyama-Honda I., Mizushima N.
    J. Cell Sci. 125:1488-1499(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiWIPI1_MOUSE
AccessioniPrimary (citable) accession number: Q8R3E3
Secondary accession number(s): Q8BGE1, Q8R1A9, Q8R1C7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: June 1, 2002
Last modified: May 11, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.