Q8R3C6 (RBM19_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable RNA-binding protein 19

Alternative name(s):
RNA-binding motif protein 19

Gene names

Name:

Rbm19

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	952 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in embryo pre-implantation development. Ref.4
Subcellular location	Nucleus › nucleolus. Nucleus › nucleoplasm. Cytoplasm. Chromosome. Note: Colocalizes with NPM1 during interphase. By late prophase, metaphase, anaphase and telophase, associates with the chromosome periphery. By telophase localizes to nucleolar precursor body (NPB) By similarity. In discrete foci distributed throughout the cytoplasm and nucleoplasm during the 4 to 8 cell stages and the morula stage, but not in the periphery of the NPB. During blastocyst development, becomes increasingly localized to the nucleolus and less to the cytoplasm. At the late blastocyst stage, localized predominantly in the nucleolus. Localized in the nucleolus during interphase and to the perichromosomal sheath during mitosis. Does not colocalize in the cytoplasm with GW182 in P-bodies. May translocate to the nucleolus upon early embryonic development. Ref.3 Ref.4
Tissue specificity	Expressed in the crypts of Lieberkuhn of the intestine (at protein level). Ref.3
Developmental stage	Expressed during early development. Expressed in the epithelium of the embryonic gut tube (at protein level). Ref.3
Disruption phenotype	Arrests embryonic development prior to implantation. Embryos at 3.5 dpc lack the mature, tripartite nucleoli, but instead, contain spheres resembling nucleolar precursor body (NPB), indicating arrest of nucleologenesis. Ref.4
Sequence similarities	Belongs to the RRM MRD1 family. Contains 6 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Cellular component	Chromosome Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	RNA-binding
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of embryonic development Inferred from mutant phenotype Ref.3. Source: UniProtKB
Cellular_component	chromosome Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from direct assay Ref.3. Source: UniProtKB nucleolus Inferred from direct assay Ref.3. Source: UniProtKB nucleoplasm Inferred from direct assay Ref.3. Source: UniProtKB
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8R3C6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8R3C6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     485-521: EANAPGSSYKKKKEAMDKANSSSSHNWNTLFMGPNAV → APAALSPPQQDCWPVDRAGDSTSSSGPLPCPCDAARF
     522-952: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 952

952

Probable RNA-binding protein 19

PRO_0000081782

Regions

Domain

2 – 79

78

RRM 1

Domain

293 – 368

76

RRM 2

Domain

400 – 478

79

RRM 3

Domain

584 – 656

73

RRM 4

Domain

722 – 803

82

RRM 5

Domain

824 – 904

81

RRM 6

Compositional bias

182 – 250

69

Asp/Glu-rich

Compositional bias

389 – 394

6

Poly-Glu

Compositional bias

671 – 717

47

Glu-rich

Amino acid modifications

Modified residue

177

1

Phosphoserine By similarity

Modified residue

179

1

Phosphoserine By similarity

Modified residue

183

1

Phosphoserine By similarity

Modified residue

928

1

Phosphoserine By similarity

Modified residue

944

1

Phosphoserine Ref.5

Natural variations

Alternative sequence

485 – 521

37

EANAP…GPNAV → APAALSPPQQDCWPVDRAGD STSSSGPLPCPCDAARF in isoform 2.

VSP_015005

Alternative sequence

522 – 952

431

Missing in isoform 2.

VSP_015006

Secondary structure

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952

Helix Strand Turn

Details...

Beta strand

400 – 405

6

Helix

413 – 421

9

Beta strand

426 – 430

5

Turn

435 – 437

3

Beta strand

442 – 450

9

Helix

451 – 460

10

Turn

461 – 463

3

Beta strand

464 – 468

5

Beta strand

470 – 475

6

Beta strand

581 – 590

10

Helix

597 – 605

9

Beta strand

610 – 614

5

Beta strand

617 – 620

4

Beta strand

622 – 627

6

Helix

629 – 639

11

Beta strand

643 – 648

6

Beta strand

650 – 655

6

Turn

656 – 659

4

Helix

666 – 670

5

Helix

675 – 677

3

Beta strand

724 – 728

5

Helix

735 – 743

9

Beta strand

748 – 757

10

Beta strand

763 – 775

13

Helix

776 – 785

10

Beta strand

797 – 799

3

Beta strand

826 – 830

5

Helix

837 – 845

9

Beta strand

850 – 854

5

Beta strand

860 – 862

3

Beta strand

867 – 875

9

Helix

876 – 887

12

Beta strand

891 – 894

4

Beta strand

898 – 901

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 91C45ADB66F8CDB2	FASTA	952	106,083
	10 20 30 40 50 60 MSRLIVKNLP NGMKEERFRQ LFAAFGTLTD CSLKFTKDGK FRKFGFIGFK SEEEAQAALN 70 80 90 100 110 120 HFHRSFIDTT RITVEFCKSF GDPSKPRAWS KHAQKSSQPK QPSQDSVPSD TKKDKKKKGP 130 140 150 160 170 180 SDLEKLKEDA KFQEFLSIHQ KRTQVATWAN DALEAKLPKA KTKASSDYLN FDSDSNSDSG 190 200 210 220 230 240 QESEEEPARE DPEEEQGLQP KAAVQKELSD MDYLKSKMVR AEVSSEDEDE EDSEDEAVNC 250 260 270 280 290 300 EEGSEEEEEE GSPASPAKQG GVSRGAVPGV LRPQEAAGKV EKPVSQKEPT TPYTVKLRGA 310 320 330 340 350 360 PFNVTEKNVI EFLAPLKPVA IRIVRNAHGN KTGYVFVDLS SEEEVKKALK CNRDYMGGRY 370 380 390 400 410 420 IEVFREKQAP TARGPPKSTT PWQGRTLGEN EEEEDLADSG RLFVRNLSYT SSEEDLEKLF 430 440 450 460 470 480 SAYGPLSELH YPIDSLTKKP KGFAFVTFMF PEHAVKAYAE VDGQVFQGRM LHVLPSTIKK 490 500 510 520 530 540 EASQEANAPG SSYKKKKEAM DKANSSSSHN WNTLFMGPNA VADAIAQKYN ATKSQVFDHE 550 560 570 580 590 600 TRGSVAVRVA LGETQLVQEV RSFLIDNGVC LDSFSQAAAE RSKTVILAKN LPAGTLAAEI 610 620 630 640 650 660 QETFSRFGSL GRVLLPEGGI TAIVEFLEPL EARKAFRHLA YSKFHHVPLY LEWAPIGVFG 670 680 690 700 710 720 AAPQKKDSQH EQPAEKAEVE QETVLDPEGE KASVEGAEAS TGKMEEEEEE EEEEEEESIP 730 740 750 760 770 780 GCTLFIKNLN FSTTEETLKG VFSKVGAIKS CTISKKKNKA GVLLSMGFGF VEYKKPEQAQ 790 800 810 820 830 840 KALKQLQGHT VDGHKLEVRI SERATKPALT STRKKQVPKK QTTSKILVRN IPFQANQREI 850 860 870 880 890 900 RELFSTFGEL KTVRLPKKMT GTGAHRGFGF VDFITKQDAK KAFNALCHST HLYGRRLVLE 910 920 930 940 950 WADSEVTVQT LRRKTARHFQ EPPKKKRSAV LDGILEQLED EDNSDGEQAL QL
	Isoform 2 [UniParc]. Checksum: 83C0C09AD3AB0BB2	FASTA	521	57,940
	10 20 30 40 50 60 MSRLIVKNLP NGMKEERFRQ LFAAFGTLTD CSLKFTKDGK FRKFGFIGFK SEEEAQAALN 70 80 90 100 110 120 HFHRSFIDTT RITVEFCKSF GDPSKPRAWS KHAQKSSQPK QPSQDSVPSD TKKDKKKKGP 130 140 150 160 170 180 SDLEKLKEDA KFQEFLSIHQ KRTQVATWAN DALEAKLPKA KTKASSDYLN FDSDSNSDSG 190 200 210 220 230 240 QESEEEPARE DPEEEQGLQP KAAVQKELSD MDYLKSKMVR AEVSSEDEDE EDSEDEAVNC 250 260 270 280 290 300 EEGSEEEEEE GSPASPAKQG GVSRGAVPGV LRPQEAAGKV EKPVSQKEPT TPYTVKLRGA 310 320 330 340 350 360 PFNVTEKNVI EFLAPLKPVA IRIVRNAHGN KTGYVFVDLS SEEEVKKALK CNRDYMGGRY 370 380 390 400 410 420 IEVFREKQAP TARGPPKSTT PWQGRTLGEN EEEEDLADSG RLFVRNLSYT SSEEDLEKLF 430 440 450 460 470 480 SAYGPLSELH YPIDSLTKKP KGFAFVTFMF PEHAVKAYAE VDGQVFQGRM LHVLPSTIKK 490 500 510 520 EASQAPAALS PPQQDCWPVD RAGDSTSSSG PLPCPCDAAR F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Colon and Mammary cancer.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 363-952 (ISOFORM 1). Strain: C57BL/6J. Tissue: Eye and Lung.
[3]	"Rbm19 is a nucleolar protein expressed in crypt/progenitor cells of the intestinal epithelium." Lorenzen J.A., Bonacci B.B., Palmer R.E., Wells C., Zhang J., Haber D.A., Goldstein A.M., Mayer A.N. Gene Expr. Patterns 6:45-56(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
[4]	"RBM19 is essential for preimplantation development in the mouse." Zhang J., Tomasini A.J., Mayer A.N. BMC Dev. Biol. 8:115-115(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, FUNCTION.
[5]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-944, MASS SPECTROMETRY. Tissue: Macrophage.
[6]	"Solution structure of the second RNA binding domain from hypothetical protein BAB23448." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 581-678.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BC025619 mRNA. Translation: AAH25619.1.
BC034010 mRNA. Translation: AAH34010.1.
AK004657 mRNA. Translation: BAB23448.1.
AK053511 mRNA. Translation: BAC35411.1.

IPI

IPI00165762.
IPI00475221.

RefSeq

NP_083038.1. NM_028762.1.

UniGene

Mm.41022.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WHW	NMR	-	A	399-484	[»]
1WHX	NMR	-	A	581-678	[»]
2CPF	NMR	-	A	724-808	[»]
2CPH	NMR	-	A	816-909	[»]

ProteinModelPortal

Q8R3C6.

SMR

Q8R3C6. Positions 4-78, 290-490, 583-678, 724-910.

ModBase

Search...

Protein-protein interaction databases

STRING

10090.ENSMUSP00000031590.

PTM databases

PhosphoSite

Q8R3C6.

Proteomic databases

PaxDb

Q8R3C6.

PRIDE

Q8R3C6.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000031590; ENSMUSP00000031590; ENSMUSG00000029594.

GeneID

74111.

KEGG

mmu:74111.

UCSC

uc008zha.1. mouse.

Organism-specific databases

CTD

9904.

MGI

MGI:1921361. Rbm19.

Phylogenomic databases

eggNOG

COG0724.

GeneTree

ENSGT00700000104575.

HOGENOM

HOG000211156.

HOVERGEN

HBG055637.

InParanoid

Q8R3C6.

KO

K14787.

OMA

VFREKNV.

OrthoDB

EOG4D7Z56.

Gene expression databases

Bgee

Q8R3C6.

CleanEx

MM_RBM19.

Genevestigator

Q8R3C6.

Family and domain databases

Gene3D

3.30.70.330. 6 hits.

InterPro

IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]

Pfam

PF00076. RRM_1. 4 hits.
[Graphical view]

SMART

SM00360. RRM. 6 hits.
[Graphical view]

PROSITE

PS50102. RRM. 6 hits.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

RBM19. mouse.

EvolutionaryTrace

Q8R3C6.

NextBio

339804.

SOURCE

Search...

Entry information

Entry name

RBM19_MOUSE

Accession

Primary (citable) accession number: Q8R3C6
Secondary accession number(s): Q8BHR0, Q9CW63

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program