Q8R3C0 (MCMBP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mini-chromosome maintenance complex-binding protein
Short name=MCM-BP
Short name=MCM-binding protein

Gene names

Name:

Mcmbp

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	642 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Associated component of the MCM complex that acts as a regulator of DNA replication. Binds to the MCM complex during late S phase and promotes the disassembly of the MCM complex from chromatin, thereby acting as a key regulator of pre-replication complex (pre-RC) unloading from replicated DNA. Can dissociate the MCM complex without addition of ATP; probably acts by destabilizing interactions of each individual subunits of the MCM complex. Required for sister chromatid cohesion By similarity.
Subunit structure	Interacts with the MCM complex: associates with the MCM3-7 complex which lacks MCM2, while it does not interact with the MCM complex when MCM2 is present (MCM2-7 complex). Interacts with the RPA complex, when composed of all RPA1, RPA2 and RPA3 components, but not with RPA1 or RPA2 alone By similarity.
Subcellular location	Nucleus By similarity. Note: Associates with chromatin. Highly associated with chromatin in G1/S and S phases, reduced binding to chromatin in G2, and further decreased binding in early M phase. It then reassociates with chromatin in late M phase. Dissociates from chromatin later than component of the MCM complex By similarity.
Sequence similarities	Belongs to the MCMBP family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA replication Mitosis
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA-dependent DNA replication Inferred from sequence or structural similarity. Source: UniProtKB S phase of mitotic cell cycle Inferred from sequence or structural similarity. Source: UniProtKB cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW sister chromatid cohesion Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	chromatin binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 642	642	Mini-chromosome maintenance complex-binding protein		PRO_0000089828
Amino acid modifications
	Modified residue	154	1	Phosphoserine By similarity
	Modified residue	160	1	Phosphothreonine By similarity
	Modified residue	298	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8R3C0 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: FD5E282DFCC4A5F3	FASTA	642	72,891
	10 20 30 40 50 60 MPCGEDWLSH PLGIVQGFFA QNGVNPDWEK KVIEYFKEKL KENNAPKWVP SLNEVPLHYL 70 80 90 100 110 120 KPNSFVKFRC MIQDMFDPEF YMGIYETVNQ NTKARVLHFG KYRDVAECGP QQELDLSSPR 130 140 150 160 170 180 STTSERQTFY CVPVPGESSW VKEAYVNANQ ARVSPSTSYT PSRHKRSYED DEDMDLQPSK 190 200 210 220 230 240 QKEQHPGSRQ AGGLGGLHWC GEPKRLETEA SSGQQLNTLN LSSPFDLNFP LPGEKGPACL 250 260 270 280 290 300 VKVYEDWDCF KVNDVLELYG VLSVDPVLSV LNSEERDASA LLDPMECTDM AEEQRVHSPP 310 320 330 340 350 360 ASLVPRIHVI LAQKLQHINP LLPTCLNKEE SRSCQFVSNF MSELSPVRAE LLGFLTHALL 370 380 390 400 410 420 GDSLAAEYLI LHLISTVYTR RDVLPLGKFT VNLSGCPQNS TFTEHLYRII QHLVPASFRL 430 440 450 460 470 480 QMTIENMNQL KLIPHKDYTA NRLVSGLLQL PNNTSLVIDE TLLEQGQLDT PGVHNVTALS 490 500 510 520 530 540 NLITWQKVDY DFSYHQMEFP CNINVLITSE GRSLLPADCQ IHLQPQLIPP NMEEYMNGLL 550 560 570 580 590 600 SAVLPSVLNK FRIYLTLLRF LDYNLSDDIT KAVEDDFVEM RKDDPQSITA DDLHQLLVVA 610 620 630 640 RFLSLSVGQT TLSRERWLRA KQLELSRKAR LQQQKSVNGN EL

References

[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor and Salivary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC122767 Genomic DNA. No translation available. AC136741 Genomic DNA. No translation available. BC025641 mRNA. Translation: AAH25641.1. BC038342 mRNA. Translation: AAH38342.1.
IPI	IPI00153724.
RefSeq	NP_666067.1. NM_145955.3.
UniGene	Mm.464029. Mm.58882.
3D structure databases
ProteinModelPortal	Q8R3C0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8R3C0.
PTM databases
PhosphoSite	Q8R3C0.
Proteomic databases
PRIDE	Q8R3C0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000057557; ENSMUSP00000062843; ENSMUSG00000048170.
GeneID	210711.
KEGG	mmu:210711.
UCSC	uc009jzh.1. mouse.
Organism-specific databases
CTD	79892.
MGI	MGI:1920977. Mcmbp.
Phylogenomic databases
eggNOG	roNOG08053.
GeneTree	ENSGT00390000017265.
HOVERGEN	HBG059839.
OMA	NNAPKWV.
OrthoDB	EOG4WM4T5.
Gene expression databases
ArrayExpress	Q8R3C0.
Bgee	Q8R3C0.
CleanEx	MM_1110007A13RIK.
Genevestigator	Q8R3C0.
GermOnline	ENSMUSG00000048170. Mus musculus.
Family and domain databases
InterPro	IPR019140. MCM_complex-bd. [Graphical view]
PANTHER	PTHR13489. PTHR13489. 1 hit.
Pfam	PF09739. DUF2044. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	373028.
SOURCE	Search...

Entry information

Entry name

MCMBP_MOUSE

Accession

Primary (citable) accession number: Q8R3C0
Secondary accession number(s): D3Z3G1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	June 1, 2002
Last modified:	September 21, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program