Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8R3B7

- BRD8_MOUSE

UniProt

Q8R3B7 - BRD8_MOUSE

Protein

Bromodomain-containing protein 8

Gene

Brd8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 2 (15 Feb 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    May act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome.

    GO - Biological processi

    1. histone H2A acetylation Source: UniProtKB
    2. histone H4 acetylation Source: UniProtKB
    3. regulation of growth Source: UniProtKB-KW
    4. regulation of transcription, DNA-templated Source: UniProtKB-KW
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chromatin regulator

    Keywords - Biological processi

    Growth regulation, Transcription, Transcription regulation

    Enzyme and pathway databases

    ReactomeiREACT_226917. HATs acetylate histones.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bromodomain-containing protein 8
    Gene namesi
    Name:Brd8
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:1925906. Brd8.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. mitochondrion Source: Ensembl
    2. NuA4 histone acetyltransferase complex Source: UniProtKB
    3. Swr1 complex Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 951951Bromodomain-containing protein 8PRO_0000211186Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei85 – 851N6-acetyllysine1 Publication
    Modified residuei460 – 4601PhosphoserineBy similarity
    Modified residuei554 – 5541N6-acetyllysine1 Publication
    Modified residuei694 – 6941PhosphoserineBy similarity
    Modified residuei710 – 7101PhosphoserineBy similarity
    Modified residuei714 – 7141PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ8R3B7.
    PRIDEiQ8R3B7.

    PTM databases

    PhosphoSiteiQ8R3B7.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8R3B7.
    BgeeiQ8R3B7.
    CleanExiMM_BRD8.
    GenevestigatoriQ8R3B7.

    Interactioni

    Subunit structurei

    Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. BRD8 isoform 2 interacts with RXRA/NR2B1 and THRB/ERBA2. Component of a SWR1-like complex By similarity.By similarity

    Protein-protein interaction databases

    BioGridi219557. 4 interactions.
    IntActiQ8R3B7. 6 interactions.
    MINTiMINT-4089380.
    STRINGi10090.ENSMUSP00000111431.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8R3B7.
    SMRiQ8R3B7. Positions 761-884.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini797 – 86771BromoPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili97 – 17175Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 bromo domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Bromodomain, Coiled coil

    Phylogenomic databases

    eggNOGiCOG5076.
    GeneTreeiENSGT00530000064262.
    HOGENOMiHOG000074125.
    HOVERGENiHBG050732.
    KOiK11321.
    OrthoDBiEOG7QZGBB.
    PhylomeDBiQ8R3B7.
    TreeFamiTF106422.

    Family and domain databases

    Gene3Di1.20.920.10. 1 hit.
    InterProiIPR001487. Bromodomain.
    [Graphical view]
    PfamiPF00439. Bromodomain. 1 hit.
    [Graphical view]
    PRINTSiPR00503. BROMODOMAIN.
    SMARTiSM00297. BROMO. 1 hit.
    [Graphical view]
    SUPFAMiSSF47370. SSF47370. 1 hit.
    PROSITEiPS50014. BROMODOMAIN_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8R3B7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MATGTGKHKL LSTGPTEPWS IREKLCLASS VMRSGDQNWV SVSRAIKPFA    50
    EPGRPPDWFS QKHCASQYSE LLETTETPKR KRGEKGEVVE TVEDVIVRKL 100
    TAERVEELKK VIKETQERYR RLKRDAELIQ AGHMDSRLDE LCNDIAMKKK 150
    LEEEEAEVKR KATDAAYQAR QAVKTPPRRL PTVMVRSPVD SASPGGDYPL 200
    GDLTPTTMEE ATSGVTPGTL PSTPVTSFPG IPDTLPPGSA PLEAPMTPIT 250
    DDSPQKKMLG QKATPPPSPL LSELLKKGSL LPTSPRLVNE SEMPVPPGHL 300
    NSTGVLLEVG GVLPMIHGGE IQPTTSAVAA SPAASGAPTL SRLLEAGPTQ 350
    FTTPLPSFTT VASEPPVKLV PPPVESVSQA TIVMMPALPA PSSAAAVSTS 400
    ESGAPVSQPE PCVPLEAVGD PHTVTVSMDS NEISMIINSI KEECFRSGVA 450
    EAPGGSKAPS IDGKEDLDLA EKMDIAVSYT GEELDFETVG DIIAIIEDKV 500
    DDHPEVLDVA AVEAALSFCE ENDDPQSLPG PWEHPIQQER DKPVPLPAPE 550
    MTVKQERLDF EESENKGLHD LVDIRDSGVE IKVEPTEPEP GMSGAEIVAG 600
    VGPVPSMEPP ELRSQDSDEE PRSSAAGDIG EADGSSGKGD ERPLSAVKTE 650
    ASPESMLSPS HGSNLIEDPL EAETQHKFEM SDSLKEESGT IFGSQIKDAP 700
    GDDEEEDGVS EAASLEEPKE EDQGEGYLSE MDNEPPVSES DDGFSIHNAT 750
    LQSHTLADSI PSSPASSQFS VCSEDQEAIQ AQKIWKKAIM LVWRAAANHR 800
    YANVFLQPVT DDIAPGYHSI VQRPMDLSTI KKNIENGLIR STAEFQRDIM 850
    LMFQNAVMYN SSDHDVYHMA VEMQRDVLEQ IQQFLATQLI MQTSESGISA 900
    KSLRGRDSTR KQDASEKDSV PMGSPAFLLS LFDGGTRGRR CAIEADMKMK 950
    K 951
    Length:951
    Mass (Da):102,610
    Last modified:February 15, 2005 - v2
    Checksum:i133859691F7CCEFB
    GO
    Isoform 2 (identifier: Q8R3B7-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         215-287: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:878
    Mass (Da):95,118
    Checksum:i7E8DC981F99436A8
    GO

    Sequence cautioni

    The sequence AAH23160.1 differs from that shown. Reason: Intron retention.
    The sequence AAH25644.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAC27812.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti811 – 8111D → G in BAC27812. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei215 – 28773Missing in isoform 2. 1 PublicationVSP_038218Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK032320 mRNA. Translation: BAC27812.1. Different initiation.
    AK161689 mRNA. Translation: BAE36533.1.
    BC023160 mRNA. Translation: AAH23160.1. Sequence problems.
    BC025644 mRNA. Translation: AAH25644.1. Different initiation.
    CCDSiCCDS50248.1. [Q8R3B7-1]
    CCDS70879.1. [Q8R3B7-2]
    RefSeqiNP_001276535.1. NM_001289606.1. [Q8R3B7-2]
    NP_001276536.1. NM_001289607.1.
    NP_084423.2. NM_030147.3. [Q8R3B7-1]
    UniGeneiMm.411740.
    Mm.45602.

    Genome annotation databases

    EnsembliENSMUST00000003876; ENSMUSP00000003876; ENSMUSG00000003778. [Q8R3B7-1]
    ENSMUST00000097626; ENSMUSP00000095229; ENSMUSG00000003778. [Q8R3B7-2]
    GeneIDi78656.
    KEGGimmu:78656.
    UCSCiuc008ekv.1. mouse. [Q8R3B7-1]
    uc008ekx.1. mouse. [Q8R3B7-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK032320 mRNA. Translation: BAC27812.1 . Different initiation.
    AK161689 mRNA. Translation: BAE36533.1 .
    BC023160 mRNA. Translation: AAH23160.1 . Sequence problems.
    BC025644 mRNA. Translation: AAH25644.1 . Different initiation.
    CCDSi CCDS50248.1. [Q8R3B7-1 ]
    CCDS70879.1. [Q8R3B7-2 ]
    RefSeqi NP_001276535.1. NM_001289606.1. [Q8R3B7-2 ]
    NP_001276536.1. NM_001289607.1.
    NP_084423.2. NM_030147.3. [Q8R3B7-1 ]
    UniGenei Mm.411740.
    Mm.45602.

    3D structure databases

    ProteinModelPortali Q8R3B7.
    SMRi Q8R3B7. Positions 761-884.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 219557. 4 interactions.
    IntActi Q8R3B7. 6 interactions.
    MINTi MINT-4089380.
    STRINGi 10090.ENSMUSP00000111431.

    PTM databases

    PhosphoSitei Q8R3B7.

    Proteomic databases

    PaxDbi Q8R3B7.
    PRIDEi Q8R3B7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000003876 ; ENSMUSP00000003876 ; ENSMUSG00000003778 . [Q8R3B7-1 ]
    ENSMUST00000097626 ; ENSMUSP00000095229 ; ENSMUSG00000003778 . [Q8R3B7-2 ]
    GeneIDi 78656.
    KEGGi mmu:78656.
    UCSCi uc008ekv.1. mouse. [Q8R3B7-1 ]
    uc008ekx.1. mouse. [Q8R3B7-2 ]

    Organism-specific databases

    CTDi 10902.
    MGIi MGI:1925906. Brd8.

    Phylogenomic databases

    eggNOGi COG5076.
    GeneTreei ENSGT00530000064262.
    HOGENOMi HOG000074125.
    HOVERGENi HBG050732.
    KOi K11321.
    OrthoDBi EOG7QZGBB.
    PhylomeDBi Q8R3B7.
    TreeFami TF106422.

    Enzyme and pathway databases

    Reactomei REACT_226917. HATs acetylate histones.

    Miscellaneous databases

    NextBioi 349290.
    PROi Q8R3B7.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8R3B7.
    Bgeei Q8R3B7.
    CleanExi MM_BRD8.
    Genevestigatori Q8R3B7.

    Family and domain databases

    Gene3Di 1.20.920.10. 1 hit.
    InterProi IPR001487. Bromodomain.
    [Graphical view ]
    Pfami PF00439. Bromodomain. 1 hit.
    [Graphical view ]
    PRINTSi PR00503. BROMODOMAIN.
    SMARTi SM00297. BROMO. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47370. SSF47370. 1 hit.
    PROSITEi PS50014. BROMODOMAIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Embryo and Olfactory bulb.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Mammary tumor.
    3. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-85 AND LYS-554, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiBRD8_MOUSE
    AccessioniPrimary (citable) accession number: Q8R3B7
    Secondary accession number(s): Q3TSZ2
    , Q8C049, Q8R583, Q8VDP0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 15, 2005
    Last sequence update: February 15, 2005
    Last modified: October 1, 2014
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3