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Q8R3B7 (BRD8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bromodomain-containing protein 8
Gene names
Name:Brd8
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length951 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome.

Subunit structure

Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. BRD8 isoform 2 interacts with RXRA/NR2B1 and THRB/ERBA2. Component of a SWR1-like complex By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Contains 1 bromo domain.

Sequence caution

The sequence AAH23160.1 differs from that shown. Reason: Intron retention.

The sequence AAH25644.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAC27812.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8R3B7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8R3B7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     215-287: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 951951Bromodomain-containing protein 8
PRO_0000211186

Regions

Domain797 – 86771Bromo
Coiled coil97 – 17175 Potential

Amino acid modifications

Modified residue851N6-acetyllysine Ref.3
Modified residue4601Phosphoserine By similarity
Modified residue5541N6-acetyllysine Ref.3
Modified residue6941Phosphoserine By similarity
Modified residue7101Phosphoserine By similarity
Modified residue7141Phosphoserine By similarity

Natural variations

Alternative sequence215 – 28773Missing in isoform 2.
VSP_038218

Experimental info

Sequence conflict8111D → G in BAC27812. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 15, 2005. Version 2.
Checksum: 133859691F7CCEFB

FASTA951102,610
        10         20         30         40         50         60 
MATGTGKHKL LSTGPTEPWS IREKLCLASS VMRSGDQNWV SVSRAIKPFA EPGRPPDWFS 

        70         80         90        100        110        120 
QKHCASQYSE LLETTETPKR KRGEKGEVVE TVEDVIVRKL TAERVEELKK VIKETQERYR 

       130        140        150        160        170        180 
RLKRDAELIQ AGHMDSRLDE LCNDIAMKKK LEEEEAEVKR KATDAAYQAR QAVKTPPRRL 

       190        200        210        220        230        240 
PTVMVRSPVD SASPGGDYPL GDLTPTTMEE ATSGVTPGTL PSTPVTSFPG IPDTLPPGSA 

       250        260        270        280        290        300 
PLEAPMTPIT DDSPQKKMLG QKATPPPSPL LSELLKKGSL LPTSPRLVNE SEMPVPPGHL 

       310        320        330        340        350        360 
NSTGVLLEVG GVLPMIHGGE IQPTTSAVAA SPAASGAPTL SRLLEAGPTQ FTTPLPSFTT 

       370        380        390        400        410        420 
VASEPPVKLV PPPVESVSQA TIVMMPALPA PSSAAAVSTS ESGAPVSQPE PCVPLEAVGD 

       430        440        450        460        470        480 
PHTVTVSMDS NEISMIINSI KEECFRSGVA EAPGGSKAPS IDGKEDLDLA EKMDIAVSYT 

       490        500        510        520        530        540 
GEELDFETVG DIIAIIEDKV DDHPEVLDVA AVEAALSFCE ENDDPQSLPG PWEHPIQQER 

       550        560        570        580        590        600 
DKPVPLPAPE MTVKQERLDF EESENKGLHD LVDIRDSGVE IKVEPTEPEP GMSGAEIVAG 

       610        620        630        640        650        660 
VGPVPSMEPP ELRSQDSDEE PRSSAAGDIG EADGSSGKGD ERPLSAVKTE ASPESMLSPS 

       670        680        690        700        710        720 
HGSNLIEDPL EAETQHKFEM SDSLKEESGT IFGSQIKDAP GDDEEEDGVS EAASLEEPKE 

       730        740        750        760        770        780 
EDQGEGYLSE MDNEPPVSES DDGFSIHNAT LQSHTLADSI PSSPASSQFS VCSEDQEAIQ 

       790        800        810        820        830        840 
AQKIWKKAIM LVWRAAANHR YANVFLQPVT DDIAPGYHSI VQRPMDLSTI KKNIENGLIR 

       850        860        870        880        890        900 
STAEFQRDIM LMFQNAVMYN SSDHDVYHMA VEMQRDVLEQ IQQFLATQLI MQTSESGISA 

       910        920        930        940        950 
KSLRGRDSTR KQDASEKDSV PMGSPAFLLS LFDGGTRGRR CAIEADMKMK K 

« Hide

Isoform 2 [UniParc].

Checksum: 7E8DC981F99436A8
Show »

FASTA87895,118

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Embryo and Olfactory bulb.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Mammary tumor.
[3]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-85 AND LYS-554, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK032320 mRNA. Translation: BAC27812.1. Different initiation.
AK161689 mRNA. Translation: BAE36533.1.
BC023160 mRNA. Translation: AAH23160.1. Sequence problems.
BC025644 mRNA. Translation: AAH25644.1. Different initiation.
CCDSCCDS50248.1. [Q8R3B7-1]
RefSeqNP_001276535.1. NM_001289606.1. [Q8R3B7-2]
NP_001276536.1. NM_001289607.1.
NP_084423.2. NM_030147.3. [Q8R3B7-1]
UniGeneMm.411740.
Mm.45602.

3D structure databases

ProteinModelPortalQ8R3B7.
SMRQ8R3B7. Positions 761-884.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid219557. 4 interactions.
IntActQ8R3B7. 6 interactions.
MINTMINT-4089380.
STRING10090.ENSMUSP00000111431.

PTM databases

PhosphoSiteQ8R3B7.

Proteomic databases

PaxDbQ8R3B7.
PRIDEQ8R3B7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000003876; ENSMUSP00000003876; ENSMUSG00000003778. [Q8R3B7-1]
ENSMUST00000097626; ENSMUSP00000095229; ENSMUSG00000003778. [Q8R3B7-2]
GeneID78656.
KEGGmmu:78656.
UCSCuc008ekv.1. mouse. [Q8R3B7-1]
uc008ekx.1. mouse. [Q8R3B7-2]

Organism-specific databases

CTD10902.
MGIMGI:1925906. Brd8.

Phylogenomic databases

eggNOGCOG5076.
GeneTreeENSGT00530000064262.
HOGENOMHOG000074125.
HOVERGENHBG050732.
KOK11321.
OrthoDBEOG7QZGBB.
PhylomeDBQ8R3B7.
TreeFamTF106422.

Gene expression databases

ArrayExpressQ8R3B7.
BgeeQ8R3B7.
CleanExMM_BRD8.
GenevestigatorQ8R3B7.

Family and domain databases

Gene3D1.20.920.10. 1 hit.
InterProIPR001487. Bromodomain.
[Graphical view]
PfamPF00439. Bromodomain. 1 hit.
[Graphical view]
PRINTSPR00503. BROMODOMAIN.
SMARTSM00297. BROMO. 1 hit.
[Graphical view]
SUPFAMSSF47370. SSF47370. 1 hit.
PROSITEPS50014. BROMODOMAIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio349290.
PROQ8R3B7.
SOURCESearch...

Entry information

Entry nameBRD8_MOUSE
AccessionPrimary (citable) accession number: Q8R3B7
Secondary accession number(s): Q3TSZ2 expand/collapse secondary AC list , Q8C049, Q8R583, Q8VDP0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: February 15, 2005
Last modified: July 9, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot