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Q8R3B7

- BRD8_MOUSE

UniProt

Q8R3B7 - BRD8_MOUSE

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Protein

Bromodomain-containing protein 8

Gene

Brd8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

May act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome.

GO - Biological processi

  1. histone H2A acetylation Source: UniProtKB
  2. histone H4 acetylation Source: UniProtKB
  3. regulation of growth Source: UniProtKB-KW
  4. regulation of transcription, DNA-templated Source: UniProtKB-KW
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

Growth regulation, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_226917. HATs acetylate histones.

Names & Taxonomyi

Protein namesi
Recommended name:
Bromodomain-containing protein 8
Gene namesi
Name:Brd8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 18

Organism-specific databases

MGIiMGI:1925906. Brd8.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. mitochondrion Source: Ensembl
  2. NuA4 histone acetyltransferase complex Source: UniProtKB
  3. Swr1 complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 951951Bromodomain-containing protein 8PRO_0000211186Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei85 – 851N6-acetyllysine1 Publication
Modified residuei460 – 4601PhosphoserineBy similarity
Modified residuei554 – 5541N6-acetyllysine1 Publication
Modified residuei694 – 6941PhosphoserineBy similarity
Modified residuei710 – 7101PhosphoserineBy similarity
Modified residuei714 – 7141PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ8R3B7.
PaxDbiQ8R3B7.
PRIDEiQ8R3B7.

PTM databases

PhosphoSiteiQ8R3B7.

Expressioni

Gene expression databases

BgeeiQ8R3B7.
CleanExiMM_BRD8.
ExpressionAtlasiQ8R3B7. baseline and differential.
GenevestigatoriQ8R3B7.

Interactioni

Subunit structurei

Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. BRD8 isoform 2 interacts with RXRA/NR2B1 and THRB/ERBA2. Component of a SWR1-like complex (By similarity).By similarity

Protein-protein interaction databases

BioGridi219557. 4 interactions.
IntActiQ8R3B7. 6 interactions.
MINTiMINT-4089380.
STRINGi10090.ENSMUSP00000111431.

Structurei

3D structure databases

ProteinModelPortaliQ8R3B7.
SMRiQ8R3B7. Positions 761-884.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini797 – 86771BromoPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili97 – 17175Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 bromo domain.PROSITE-ProRule annotation

Keywords - Domaini

Bromodomain, Coiled coil

Phylogenomic databases

eggNOGiCOG5076.
GeneTreeiENSGT00530000064262.
HOGENOMiHOG000074125.
HOVERGENiHBG050732.
InParanoidiQ8R3B7.
KOiK11321.
OrthoDBiEOG7QZGBB.
PhylomeDBiQ8R3B7.
TreeFamiTF106422.

Family and domain databases

Gene3Di1.20.920.10. 1 hit.
InterProiIPR001487. Bromodomain.
[Graphical view]
PfamiPF00439. Bromodomain. 1 hit.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 1 hit.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 1 hit.
PROSITEiPS50014. BROMODOMAIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8R3B7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATGTGKHKL LSTGPTEPWS IREKLCLASS VMRSGDQNWV SVSRAIKPFA
60 70 80 90 100
EPGRPPDWFS QKHCASQYSE LLETTETPKR KRGEKGEVVE TVEDVIVRKL
110 120 130 140 150
TAERVEELKK VIKETQERYR RLKRDAELIQ AGHMDSRLDE LCNDIAMKKK
160 170 180 190 200
LEEEEAEVKR KATDAAYQAR QAVKTPPRRL PTVMVRSPVD SASPGGDYPL
210 220 230 240 250
GDLTPTTMEE ATSGVTPGTL PSTPVTSFPG IPDTLPPGSA PLEAPMTPIT
260 270 280 290 300
DDSPQKKMLG QKATPPPSPL LSELLKKGSL LPTSPRLVNE SEMPVPPGHL
310 320 330 340 350
NSTGVLLEVG GVLPMIHGGE IQPTTSAVAA SPAASGAPTL SRLLEAGPTQ
360 370 380 390 400
FTTPLPSFTT VASEPPVKLV PPPVESVSQA TIVMMPALPA PSSAAAVSTS
410 420 430 440 450
ESGAPVSQPE PCVPLEAVGD PHTVTVSMDS NEISMIINSI KEECFRSGVA
460 470 480 490 500
EAPGGSKAPS IDGKEDLDLA EKMDIAVSYT GEELDFETVG DIIAIIEDKV
510 520 530 540 550
DDHPEVLDVA AVEAALSFCE ENDDPQSLPG PWEHPIQQER DKPVPLPAPE
560 570 580 590 600
MTVKQERLDF EESENKGLHD LVDIRDSGVE IKVEPTEPEP GMSGAEIVAG
610 620 630 640 650
VGPVPSMEPP ELRSQDSDEE PRSSAAGDIG EADGSSGKGD ERPLSAVKTE
660 670 680 690 700
ASPESMLSPS HGSNLIEDPL EAETQHKFEM SDSLKEESGT IFGSQIKDAP
710 720 730 740 750
GDDEEEDGVS EAASLEEPKE EDQGEGYLSE MDNEPPVSES DDGFSIHNAT
760 770 780 790 800
LQSHTLADSI PSSPASSQFS VCSEDQEAIQ AQKIWKKAIM LVWRAAANHR
810 820 830 840 850
YANVFLQPVT DDIAPGYHSI VQRPMDLSTI KKNIENGLIR STAEFQRDIM
860 870 880 890 900
LMFQNAVMYN SSDHDVYHMA VEMQRDVLEQ IQQFLATQLI MQTSESGISA
910 920 930 940 950
KSLRGRDSTR KQDASEKDSV PMGSPAFLLS LFDGGTRGRR CAIEADMKMK

K
Length:951
Mass (Da):102,610
Last modified:February 15, 2005 - v2
Checksum:i133859691F7CCEFB
GO
Isoform 2 (identifier: Q8R3B7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     215-287: Missing.

Note: No experimental confirmation available.

Show »
Length:878
Mass (Da):95,118
Checksum:i7E8DC981F99436A8
GO

Sequence cautioni

The sequence AAH23160.1 differs from that shown. Reason: Intron retention.
The sequence AAH25644.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAC27812.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti811 – 8111D → G in BAC27812. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei215 – 28773Missing in isoform 2. 1 PublicationVSP_038218Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK032320 mRNA. Translation: BAC27812.1. Different initiation.
AK161689 mRNA. Translation: BAE36533.1.
BC023160 mRNA. Translation: AAH23160.1. Sequence problems.
BC025644 mRNA. Translation: AAH25644.1. Different initiation.
CCDSiCCDS50248.1. [Q8R3B7-1]
CCDS70879.1. [Q8R3B7-2]
RefSeqiNP_001276535.1. NM_001289606.1. [Q8R3B7-2]
NP_001276536.1. NM_001289607.1.
NP_084423.2. NM_030147.3. [Q8R3B7-1]
UniGeneiMm.411740.
Mm.45602.

Genome annotation databases

EnsembliENSMUST00000003876; ENSMUSP00000003876; ENSMUSG00000003778. [Q8R3B7-1]
ENSMUST00000097626; ENSMUSP00000095229; ENSMUSG00000003778. [Q8R3B7-2]
GeneIDi78656.
KEGGimmu:78656.
UCSCiuc008ekv.1. mouse. [Q8R3B7-1]
uc008ekx.1. mouse. [Q8R3B7-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK032320 mRNA. Translation: BAC27812.1 . Different initiation.
AK161689 mRNA. Translation: BAE36533.1 .
BC023160 mRNA. Translation: AAH23160.1 . Sequence problems.
BC025644 mRNA. Translation: AAH25644.1 . Different initiation.
CCDSi CCDS50248.1. [Q8R3B7-1 ]
CCDS70879.1. [Q8R3B7-2 ]
RefSeqi NP_001276535.1. NM_001289606.1. [Q8R3B7-2 ]
NP_001276536.1. NM_001289607.1.
NP_084423.2. NM_030147.3. [Q8R3B7-1 ]
UniGenei Mm.411740.
Mm.45602.

3D structure databases

ProteinModelPortali Q8R3B7.
SMRi Q8R3B7. Positions 761-884.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 219557. 4 interactions.
IntActi Q8R3B7. 6 interactions.
MINTi MINT-4089380.
STRINGi 10090.ENSMUSP00000111431.

PTM databases

PhosphoSitei Q8R3B7.

Proteomic databases

MaxQBi Q8R3B7.
PaxDbi Q8R3B7.
PRIDEi Q8R3B7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000003876 ; ENSMUSP00000003876 ; ENSMUSG00000003778 . [Q8R3B7-1 ]
ENSMUST00000097626 ; ENSMUSP00000095229 ; ENSMUSG00000003778 . [Q8R3B7-2 ]
GeneIDi 78656.
KEGGi mmu:78656.
UCSCi uc008ekv.1. mouse. [Q8R3B7-1 ]
uc008ekx.1. mouse. [Q8R3B7-2 ]

Organism-specific databases

CTDi 10902.
MGIi MGI:1925906. Brd8.

Phylogenomic databases

eggNOGi COG5076.
GeneTreei ENSGT00530000064262.
HOGENOMi HOG000074125.
HOVERGENi HBG050732.
InParanoidi Q8R3B7.
KOi K11321.
OrthoDBi EOG7QZGBB.
PhylomeDBi Q8R3B7.
TreeFami TF106422.

Enzyme and pathway databases

Reactomei REACT_226917. HATs acetylate histones.

Miscellaneous databases

NextBioi 349290.
PROi Q8R3B7.
SOURCEi Search...

Gene expression databases

Bgeei Q8R3B7.
CleanExi MM_BRD8.
ExpressionAtlasi Q8R3B7. baseline and differential.
Genevestigatori Q8R3B7.

Family and domain databases

Gene3Di 1.20.920.10. 1 hit.
InterProi IPR001487. Bromodomain.
[Graphical view ]
Pfami PF00439. Bromodomain. 1 hit.
[Graphical view ]
PRINTSi PR00503. BROMODOMAIN.
SMARTi SM00297. BROMO. 1 hit.
[Graphical view ]
SUPFAMi SSF47370. SSF47370. 1 hit.
PROSITEi PS50014. BROMODOMAIN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Embryo and Olfactory bulb.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-85 AND LYS-554, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiBRD8_MOUSE
AccessioniPrimary (citable) accession number: Q8R3B7
Secondary accession number(s): Q3TSZ2
, Q8C049, Q8R583, Q8VDP0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: February 15, 2005
Last modified: October 29, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3