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Protein

Bromodomain-containing protein 8

Gene

Brd8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

Growth regulation, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Bromodomain-containing protein 8
Gene namesi
Name:Brd8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1925906. Brd8.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002111861 – 951Bromodomain-containing protein 8Add BLAST951

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei85N6-acetyllysineCombined sources1
Modified residuei456PhosphoserineCombined sources1
Modified residuei460PhosphoserineCombined sources1
Modified residuei554N6-acetyllysine; alternateCombined sources1
Cross-linki554Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki554Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Cross-linki582Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki648Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei652PhosphoserineBy similarity1
Cross-linki685Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei694PhosphoserineBy similarity1
Modified residuei710PhosphoserineBy similarity1
Modified residuei714PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8R3B7.
PeptideAtlasiQ8R3B7.
PRIDEiQ8R3B7.

PTM databases

iPTMnetiQ8R3B7.
PhosphoSitePlusiQ8R3B7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000003778.
CleanExiMM_BRD8.
ExpressionAtlasiQ8R3B7. baseline and differential.
GenevisibleiQ8R3B7. MM.

Interactioni

Subunit structurei

Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. BRD8 isoform 2 interacts with RXRA/NR2B1 and THRB/ERBA2. Component of a SWR1-like complex (By similarity).By similarity

Protein-protein interaction databases

BioGridi219557. 5 interactors.
IntActiQ8R3B7. 6 interactors.
MINTiMINT-4089380.
STRINGi10090.ENSMUSP00000003876.

Structurei

3D structure databases

ProteinModelPortaliQ8R3B7.
SMRiQ8R3B7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini797 – 867BromoPROSITE-ProRule annotationAdd BLAST71

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili97 – 171Sequence analysisAdd BLAST75

Sequence similaritiesi

Contains 1 bromo domain.PROSITE-ProRule annotation

Keywords - Domaini

Bromodomain, Coiled coil

Phylogenomic databases

eggNOGiENOG410IF07. Eukaryota.
ENOG410Y97J. LUCA.
GeneTreeiENSGT00530000064262.
HOGENOMiHOG000074125.
HOVERGENiHBG050732.
InParanoidiQ8R3B7.
KOiK11321.
PhylomeDBiQ8R3B7.
TreeFamiTF106422.

Family and domain databases

Gene3Di1.20.920.10. 1 hit.
InterProiIPR001487. Bromodomain.
[Graphical view]
PfamiPF00439. Bromodomain. 1 hit.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 1 hit.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 1 hit.
PROSITEiPS50014. BROMODOMAIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R3B7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATGTGKHKL LSTGPTEPWS IREKLCLASS VMRSGDQNWV SVSRAIKPFA
60 70 80 90 100
EPGRPPDWFS QKHCASQYSE LLETTETPKR KRGEKGEVVE TVEDVIVRKL
110 120 130 140 150
TAERVEELKK VIKETQERYR RLKRDAELIQ AGHMDSRLDE LCNDIAMKKK
160 170 180 190 200
LEEEEAEVKR KATDAAYQAR QAVKTPPRRL PTVMVRSPVD SASPGGDYPL
210 220 230 240 250
GDLTPTTMEE ATSGVTPGTL PSTPVTSFPG IPDTLPPGSA PLEAPMTPIT
260 270 280 290 300
DDSPQKKMLG QKATPPPSPL LSELLKKGSL LPTSPRLVNE SEMPVPPGHL
310 320 330 340 350
NSTGVLLEVG GVLPMIHGGE IQPTTSAVAA SPAASGAPTL SRLLEAGPTQ
360 370 380 390 400
FTTPLPSFTT VASEPPVKLV PPPVESVSQA TIVMMPALPA PSSAAAVSTS
410 420 430 440 450
ESGAPVSQPE PCVPLEAVGD PHTVTVSMDS NEISMIINSI KEECFRSGVA
460 470 480 490 500
EAPGGSKAPS IDGKEDLDLA EKMDIAVSYT GEELDFETVG DIIAIIEDKV
510 520 530 540 550
DDHPEVLDVA AVEAALSFCE ENDDPQSLPG PWEHPIQQER DKPVPLPAPE
560 570 580 590 600
MTVKQERLDF EESENKGLHD LVDIRDSGVE IKVEPTEPEP GMSGAEIVAG
610 620 630 640 650
VGPVPSMEPP ELRSQDSDEE PRSSAAGDIG EADGSSGKGD ERPLSAVKTE
660 670 680 690 700
ASPESMLSPS HGSNLIEDPL EAETQHKFEM SDSLKEESGT IFGSQIKDAP
710 720 730 740 750
GDDEEEDGVS EAASLEEPKE EDQGEGYLSE MDNEPPVSES DDGFSIHNAT
760 770 780 790 800
LQSHTLADSI PSSPASSQFS VCSEDQEAIQ AQKIWKKAIM LVWRAAANHR
810 820 830 840 850
YANVFLQPVT DDIAPGYHSI VQRPMDLSTI KKNIENGLIR STAEFQRDIM
860 870 880 890 900
LMFQNAVMYN SSDHDVYHMA VEMQRDVLEQ IQQFLATQLI MQTSESGISA
910 920 930 940 950
KSLRGRDSTR KQDASEKDSV PMGSPAFLLS LFDGGTRGRR CAIEADMKMK

K
Length:951
Mass (Da):102,610
Last modified:February 15, 2005 - v2
Checksum:i133859691F7CCEFB
GO
Isoform 2 (identifier: Q8R3B7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     215-287: Missing.

Note: No experimental confirmation available.
Show »
Length:878
Mass (Da):95,118
Checksum:i7E8DC981F99436A8
GO

Sequence cautioni

The sequence AAH23160 differs from that shown. Intron retention.Curated
The sequence AAH25644 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC27812 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti811D → G in BAC27812 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_038218215 – 287Missing in isoform 2. 1 PublicationAdd BLAST73

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK032320 mRNA. Translation: BAC27812.1. Different initiation.
AK161689 mRNA. Translation: BAE36533.1.
BC023160 mRNA. Translation: AAH23160.1. Sequence problems.
BC025644 mRNA. Translation: AAH25644.1. Different initiation.
CCDSiCCDS50248.1. [Q8R3B7-1]
CCDS70879.1. [Q8R3B7-2]
RefSeqiNP_001276535.1. NM_001289606.1. [Q8R3B7-2]
NP_001276536.1. NM_001289607.1.
NP_084423.2. NM_030147.3. [Q8R3B7-1]
UniGeneiMm.411740.
Mm.45602.

Genome annotation databases

EnsembliENSMUST00000003876; ENSMUSP00000003876; ENSMUSG00000003778. [Q8R3B7-1]
ENSMUST00000097626; ENSMUSP00000095229; ENSMUSG00000003778. [Q8R3B7-2]
GeneIDi78656.
KEGGimmu:78656.
UCSCiuc008ekv.2. mouse. [Q8R3B7-1]
uc008ekx.2. mouse. [Q8R3B7-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK032320 mRNA. Translation: BAC27812.1. Different initiation.
AK161689 mRNA. Translation: BAE36533.1.
BC023160 mRNA. Translation: AAH23160.1. Sequence problems.
BC025644 mRNA. Translation: AAH25644.1. Different initiation.
CCDSiCCDS50248.1. [Q8R3B7-1]
CCDS70879.1. [Q8R3B7-2]
RefSeqiNP_001276535.1. NM_001289606.1. [Q8R3B7-2]
NP_001276536.1. NM_001289607.1.
NP_084423.2. NM_030147.3. [Q8R3B7-1]
UniGeneiMm.411740.
Mm.45602.

3D structure databases

ProteinModelPortaliQ8R3B7.
SMRiQ8R3B7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219557. 5 interactors.
IntActiQ8R3B7. 6 interactors.
MINTiMINT-4089380.
STRINGi10090.ENSMUSP00000003876.

PTM databases

iPTMnetiQ8R3B7.
PhosphoSitePlusiQ8R3B7.

Proteomic databases

PaxDbiQ8R3B7.
PeptideAtlasiQ8R3B7.
PRIDEiQ8R3B7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003876; ENSMUSP00000003876; ENSMUSG00000003778. [Q8R3B7-1]
ENSMUST00000097626; ENSMUSP00000095229; ENSMUSG00000003778. [Q8R3B7-2]
GeneIDi78656.
KEGGimmu:78656.
UCSCiuc008ekv.2. mouse. [Q8R3B7-1]
uc008ekx.2. mouse. [Q8R3B7-2]

Organism-specific databases

CTDi10902.
MGIiMGI:1925906. Brd8.

Phylogenomic databases

eggNOGiENOG410IF07. Eukaryota.
ENOG410Y97J. LUCA.
GeneTreeiENSGT00530000064262.
HOGENOMiHOG000074125.
HOVERGENiHBG050732.
InParanoidiQ8R3B7.
KOiK11321.
PhylomeDBiQ8R3B7.
TreeFamiTF106422.

Miscellaneous databases

ChiTaRSiBrd8. mouse.
PROiQ8R3B7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000003778.
CleanExiMM_BRD8.
ExpressionAtlasiQ8R3B7. baseline and differential.
GenevisibleiQ8R3B7. MM.

Family and domain databases

Gene3Di1.20.920.10. 1 hit.
InterProiIPR001487. Bromodomain.
[Graphical view]
PfamiPF00439. Bromodomain. 1 hit.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 1 hit.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 1 hit.
PROSITEiPS50014. BROMODOMAIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBRD8_MOUSE
AccessioniPrimary (citable) accession number: Q8R3B7
Secondary accession number(s): Q3TSZ2
, Q8C049, Q8R583, Q8VDP0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: February 15, 2005
Last modified: November 2, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.