Q8R3B7 (BRD8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bromodomain-containing protein 8

Gene names

Name:

Brd8

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	951 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage.
Subunit structure	Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. BRD8 isoform 2 interacts with RXRA/NR2B1 and THRB/ERBA2 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Contains 1 bromo domain.
Sequence caution	The sequence AAH23160.1 differs from that shown. Reason: Intron retention. The sequence AAH25644.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC27812.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Growth regulation Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Bromodomain Coiled coil
Molecular function	Chromatin regulator
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	histone H2A acetylation Inferred from sequence or structural similarity. Source: UniProtKB histone H4 acetylation Inferred from sequence or structural similarity. Source: UniProtKB regulation of growth Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	NuA4 histone acetyltransferase complex Inferred from sequence or structural similarity. Source: UniProtKB mitochondrion Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8R3B7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8R3B7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     215-287: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 951	951	Bromodomain-containing protein 8		PRO_0000211186
Regions
	Domain	797 – 867	71	Bromo
	Coiled coil	97 – 171	75	Potential
Amino acid modifications
	Modified residue	460	1	Phosphoserine By similarity
	Modified residue	554	1	N6-acetyllysine By similarity
	Modified residue	694	1	Phosphoserine By similarity
	Modified residue	710	1	Phosphoserine Ref.3
	Modified residue	714	1	Phosphoserine Ref.3
Natural variations
	Alternative sequence	215 – 287	73	Missing in isoform 2.		VSP_038218
Experimental info
	Sequence conflict	811	1	D → G in BAC27812. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 15, 2005. Version 2. Checksum: 133859691F7CCEFB	FASTA	951	102,610
	10 20 30 40 50 60 MATGTGKHKL LSTGPTEPWS IREKLCLASS VMRSGDQNWV SVSRAIKPFA EPGRPPDWFS 70 80 90 100 110 120 QKHCASQYSE LLETTETPKR KRGEKGEVVE TVEDVIVRKL TAERVEELKK VIKETQERYR 130 140 150 160 170 180 RLKRDAELIQ AGHMDSRLDE LCNDIAMKKK LEEEEAEVKR KATDAAYQAR QAVKTPPRRL 190 200 210 220 230 240 PTVMVRSPVD SASPGGDYPL GDLTPTTMEE ATSGVTPGTL PSTPVTSFPG IPDTLPPGSA 250 260 270 280 290 300 PLEAPMTPIT DDSPQKKMLG QKATPPPSPL LSELLKKGSL LPTSPRLVNE SEMPVPPGHL 310 320 330 340 350 360 NSTGVLLEVG GVLPMIHGGE IQPTTSAVAA SPAASGAPTL SRLLEAGPTQ FTTPLPSFTT 370 380 390 400 410 420 VASEPPVKLV PPPVESVSQA TIVMMPALPA PSSAAAVSTS ESGAPVSQPE PCVPLEAVGD 430 440 450 460 470 480 PHTVTVSMDS NEISMIINSI KEECFRSGVA EAPGGSKAPS IDGKEDLDLA EKMDIAVSYT 490 500 510 520 530 540 GEELDFETVG DIIAIIEDKV DDHPEVLDVA AVEAALSFCE ENDDPQSLPG PWEHPIQQER 550 560 570 580 590 600 DKPVPLPAPE MTVKQERLDF EESENKGLHD LVDIRDSGVE IKVEPTEPEP GMSGAEIVAG 610 620 630 640 650 660 VGPVPSMEPP ELRSQDSDEE PRSSAAGDIG EADGSSGKGD ERPLSAVKTE ASPESMLSPS 670 680 690 700 710 720 HGSNLIEDPL EAETQHKFEM SDSLKEESGT IFGSQIKDAP GDDEEEDGVS EAASLEEPKE 730 740 750 760 770 780 EDQGEGYLSE MDNEPPVSES DDGFSIHNAT LQSHTLADSI PSSPASSQFS VCSEDQEAIQ 790 800 810 820 830 840 AQKIWKKAIM LVWRAAANHR YANVFLQPVT DDIAPGYHSI VQRPMDLSTI KKNIENGLIR 850 860 870 880 890 900 STAEFQRDIM LMFQNAVMYN SSDHDVYHMA VEMQRDVLEQ IQQFLATQLI MQTSESGISA 910 920 930 940 950 KSLRGRDSTR KQDASEKDSV PMGSPAFLLS LFDGGTRGRR CAIEADMKMK K
	Isoform 2 [UniParc]. Checksum: 7E8DC981F99436A8	FASTA	878	95,118
	10 20 30 40 50 60 MATGTGKHKL LSTGPTEPWS IREKLCLASS VMRSGDQNWV SVSRAIKPFA EPGRPPDWFS 70 80 90 100 110 120 QKHCASQYSE LLETTETPKR KRGEKGEVVE TVEDVIVRKL TAERVEELKK VIKETQERYR 130 140 150 160 170 180 RLKRDAELIQ AGHMDSRLDE LCNDIAMKKK LEEEEAEVKR KATDAAYQAR QAVKTPPRRL 190 200 210 220 230 240 PTVMVRSPVD SASPGGDYPL GDLTPTTMEE ATSGVNESEM PVPPGHLNST GVLLEVGGVL 250 260 270 280 290 300 PMIHGGEIQP TTSAVAASPA ASGAPTLSRL LEAGPTQFTT PLPSFTTVAS EPPVKLVPPP 310 320 330 340 350 360 VESVSQATIV MMPALPAPSS AAAVSTSESG APVSQPEPCV PLEAVGDPHT VTVSMDSNEI 370 380 390 400 410 420 SMIINSIKEE CFRSGVAEAP GGSKAPSIDG KEDLDLAEKM DIAVSYTGEE LDFETVGDII 430 440 450 460 470 480 AIIEDKVDDH PEVLDVAAVE AALSFCEEND DPQSLPGPWE HPIQQERDKP VPLPAPEMTV 490 500 510 520 530 540 KQERLDFEES ENKGLHDLVD IRDSGVEIKV EPTEPEPGMS GAEIVAGVGP VPSMEPPELR 550 560 570 580 590 600 SQDSDEEPRS SAAGDIGEAD GSSGKGDERP LSAVKTEASP ESMLSPSHGS NLIEDPLEAE 610 620 630 640 650 660 TQHKFEMSDS LKEESGTIFG SQIKDAPGDD EEEDGVSEAA SLEEPKEEDQ GEGYLSEMDN 670 680 690 700 710 720 EPPVSESDDG FSIHNATLQS HTLADSIPSS PASSQFSVCS EDQEAIQAQK IWKKAIMLVW 730 740 750 760 770 780 RAAANHRYAN VFLQPVTDDI APGYHSIVQR PMDLSTIKKN IENGLIRSTA EFQRDIMLMF 790 800 810 820 830 840 QNAVMYNSSD HDVYHMAVEM QRDVLEQIQQ FLATQLIMQT SESGISAKSL RGRDSTRKQD 850 860 870 ASEKDSVPMG SPAFLLSLFD GGTRGRRCAI EADMKMKK

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Embryo and Olfactory bulb.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Mammary tumor.
[3]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-710 AND SER-714, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK032320 mRNA. Translation: BAC27812.1. Different initiation. AK161689 mRNA. Translation: BAE36533.1. BC023160 mRNA. Translation: AAH23160.1. Sequence problems. BC025644 mRNA. Translation: AAH25644.1. Different initiation.
IPI	IPI00153722. IPI00653134.
RefSeq	NP_084423.2. NM_030147.2.
UniGene	Mm.411740. Mm.45602.
3D structure databases
ProteinModelPortal	Q8R3B7.
SMR	Q8R3B7. Positions 761-884.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8R3B7. 5 interactions.
STRING	10090.ENSMUSP00000111431.
PTM databases
PhosphoSite	Q8R3B7.
Proteomic databases
PaxDb	Q8R3B7.
PRIDE	Q8R3B7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000003876; ENSMUSP00000003876; ENSMUSG00000003778. ENSMUST00000097626; ENSMUSP00000095229; ENSMUSG00000003778.
GeneID	78656.
KEGG	mmu:78656.
UCSC	uc008ekv.1. mouse. uc008ekx.1. mouse.
Organism-specific databases
CTD	10902.
MGI	MGI:1925906. Brd8.
Phylogenomic databases
eggNOG	COG5076.
GeneTree	ENSGT00530000064262.
HOGENOM	HOG000074125.
HOVERGEN	HBG050732.
KO	K11321.
OrthoDB	EOG470THC.
Gene expression databases
ArrayExpress	Q8R3B7.
Bgee	Q8R3B7.
CleanEx	MM_BRD8.
Genevestigator	Q8R3B7.
GermOnline	ENSMUSG00000003778. Mus musculus.
Family and domain databases
Gene3D	1.20.920.10. 1 hit.
InterPro	IPR001487. Bromodomain. [Graphical view]
Pfam	PF00439. Bromodomain. 1 hit. [Graphical view]
PRINTS	PR00503. BROMODOMAIN.
SMART	SM00297. BROMO. 1 hit. [Graphical view]
SUPFAM	SSF47370. Bromodomain. 1 hit.
PROSITE	PS00633. BROMODOMAIN_1. False negative. PS50014. BROMODOMAIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	349290.
SOURCE	Search...

Entry information

Entry name

BRD8_MOUSE

Accession

Primary (citable) accession number: Q8R3B7
Secondary accession number(s): Q3TSZ2 , Q8C049, Q8R583, Q8VDP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	February 15, 2005
Last modified:	May 1, 2013
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program