1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-1

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Reviewed, UniProtKB/Swiss-Prot Q8R3B1 (PLCD1_MOUSE)

Last modified October 13, 2009. Version 77. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-1
    EC=3.1.4.11
Alternative name(s):
    Phosphoinositide phospholipase C
    Phospholipase C-delta-1
      Short name=PLC-delta-1
    PLC-III

Gene names

Name:	Plcd1
Synonyms:	Plcd

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length	756 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Essential for trophoblast and placental development. Ref.2
Catalytic activity	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H₂O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.
Cofactor	Binds 3 calcium ions per subunit. Two of the calcium ions are bound to the C2 domain By similarity.
Disruption phenotype	Mice lacking Plcd1 and Plcd3 die at embryonic day 11.5 (E11.5) to E13.5 and exhibit severe disruption of the normal labyrinth architecture in the placenta and decreased placental vascularization, as well as abnormal proliferation and apoptosis of trophoblasts in the labyrinth area. Furthermore, Plcd1 and Plcd3 double-knockout embryos supplied with a normal placenta by the tetraploid aggregation method survive beyond E14.5, clearly indicating that the embryonic lethality is caused by a defect in trophoblasts. Ref.2
Sequence similarities	Contains 1 C2 domain. Contains 2 EF-hand domains. Contains 1 PH domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain.

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Ontologies

Keywords
Biological process	Lipid degradation
Domain	Repeat
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Transducer
Gene Ontology (GO)
Biological process	angiogenesis Ref.2 Inferred from genetic interaction. Source: MGI intracellular signaling cascade Inferred from electronic annotation. Source: InterPro labyrinthine layer blood vessel development Ref.2 Inferred from genetic interaction. Source: MGI lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell proliferation Ref.2 Inferred from genetic interaction. Source: MGI
Cellular component	cytosol Inferred from direct assay. Source: MGI
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoinositide phospholipase C activity Inferred from electronic annotation. Source: EC signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 756

756

1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-1

PRO_0000088505

Regions

Domain

21 – 130

110

Domain

140 – 175

EF-hand 1

Domain

176 – 211

EF-hand 2

Domain

296 – 440

145

PI-PLC X-box

Domain

492 – 609

118

PI-PLC Y-box

Domain

630 – 720

Calcium binding

153 – 164

1 Potential

Calcium binding

189 – 200

2 Potential

Region

30 – 57

Substrate binding By similarity

Sites

Active site

311

By similarity

Active site

356

By similarity

Metal binding

312

Calcium 1; catalytic By similarity

Metal binding

341

Calcium 1; catalytic By similarity

Metal binding

343

Calcium 1; catalytic By similarity

Metal binding

390

Calcium 1; catalytic By similarity

Metal binding

651

Calcium 2; via carbonyl oxygen By similarity

Metal binding

653

Calcium 2 By similarity

Metal binding

677

Calcium 2 By similarity

Metal binding

706

Calcium 3 By similarity

Metal binding

707

Calcium 3; via carbonyl oxygen By similarity

Metal binding

708

Calcium 3 By similarity

Binding site

438

Substrate By similarity

Binding site

440

Substrate By similarity

Binding site

522

Substrate By similarity

Binding site

549

Substrate By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

Q8R3B1-1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 74AB0C33E1A0B938
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FASTA

756

85,901

        10         20         30         40         50         60 
MDSGRDFLTL HGLQDDPDLQ ALLKGSQLLK VKSSSWRRER FYKLQEDCKT IWQESRKVMR 

        70         80         90        100        110        120 
SPESQLFSIE DIQEVRMGHR TEGLEKFARD IPEDRCFSIV FKDQRNTLDL IAPSPADVQH 

       130        140        150        160        170        180 
WVQGLRKIID RSGSMDQRQK LQHWIHSCLR KADKNKDNKM NFKEVKDFLK ELNVQVDDSY 

       190        200        210        220        230        240 
ARKIFRECDH SQTDSLEDEE IETFYRMLTQ RVEIDRAFAE AAGSAETLSV EKLVTFLQHQ 

       250        260        270        280        290        300 
QREEEAGPAL ALSLIERYEP SETAKAQRQM TKDGFLMYLL SADGNAFSLA HRRVYQDMNQ 

       310        320        330        340        350        360 
PLSHYLVSSS HNTYLLEDQL TGPSSTEAYI RALCKGCRCL ELDCWDGPNQ EPIIYHGYTF 

       370        380        390        400        410        420 
TSKILFCDVL RAIRDYAFKA SPYPVILSLE NHCSLEQQRV MAHHLRAILG PMLLDQPLDG 

       430        440        450        460        470        480 
VTTSLPSPEQ LKEKILLKGK KLGGLLPAGG ENGPEATDVS DEDEAAEMED EAVRSQVQHK 

       490        500        510        520        530        540 
PKEDKLKLVP ELSDMVIYCK SVHFGGFSSP STSGQAFYEM ASFSESRALR LLQESGNSFV 

       550        560        570        580        590        600 
RHNVGHLSRI YPAGWRTDSS NYSPVEMWNG GCQIVALNFQ TPGPEMDVYL GCFQDNGGCG 

       610        620        630        640        650        660 
YVLKPAFLRD PDTTFNSRAL TQGPWWAPKK LRVWIISGQQ LPKVNKNKNS IVDPKVIVEI 

       670        680        690        700        710        720 
HGVGQDVASR QTAVITNNGF NPRWDTEFEF VVAVPDLALV RFMVEDYDSS SKNDFIGQST 

       730        740        750 
IPWNSLKQGY RHVHLLSKNG DLHPSATLFV KISIQD

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"Phospholipase C-delta1 and -delta3 are essential in the trophoblast for placental development." Nakamura Y., Hamada Y., Fujiwara T., Enomoto H., Hiroe T., Tanaka S., Nose M., Nakahara M., Yoshida N., Takenawa T., Fukami K. Mol. Cell. Biol. 25:10979-10988(2005) [PubMed: 16314520] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.

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Cross-references

Sequence databases
	BC025798 mRNA. Translation: AAH25798.1.
IPI	IPI00321515.
UniGene	Mm.23963
3D structure databases
HSSP	HSSP built from PDB template 1MAI based on UniProtKB P10688.
SMR	Q8R3B1. Positions 12-129, 158-756.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8R3B1.
PTM databases
PhosphoSite	Q8R3B1.
Proteomic databases
PRIDE	Q8R3B1.
Genome annotation databases
Ensembl	ENSMUST00000010804; ENSMUSP00000010804; ENSMUSG00000010660; Mus musculus. [Genome view]
Organism-specific databases
MGI	MGI:97614. Plcd1.
Phylogenomic databases
HOGENOM	Q8R3B1.
HOVERGEN	Q8R3B1.
Enzyme and pathway databases
BRENDA	3.1.4.11. 244.
Gene expression databases
ArrayExpress	Q8R3B1.
Bgee	Q8R3B1.
CleanEx	MM_PLCD1.
Genevestigator	Q8R3B1.
GermOnline	ENSMUSG00000010660. Mus musculus.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR018029. C2_membr_targeting. IPR011992. EF-Hand_type. IPR018247. EF_HAND_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca_bd. IPR011993. PH_type. IPR015359. Phospholipase_C_EF-hand-like. IPR001192. Phospholipase_C_Pinositol-sp_C. IPR000909. Phospholipase_C_Pinositol-sp_X. IPR001711. Phospholipase_C_Pinositol-sp_Y. IPR017946. PLC-like_Pdiesterase_TIM-brl. IPR001849. Pleckstrin_homology. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 2 hits. G3DSA:2.30.29.30. PH_type. 1 hit. G3DSA:3.20.20.190. PLC-like_Pdiesterase_TIM-brl. 1 hit.
Pfam	PF00168. C2. 1 hit. PF09279. efhand_like. 1 hit. PF00169. PH. 1 hit. PF00388. PI-PLC-X. 1 hit. PF00387. PI-PLC-Y. 1 hit. [Graphical view]
PRINTS	PR00390. PHPHLIPASEC.
ProDom	PD001202. PI_PLC_Y. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00239. C2. 1 hit. SM00054. EFh. 2 hits. SM00233. PH. 1 hit. SM00148. PLCXc. 1 hit. SM00149. PLCYc. 1 hit. [Graphical view]
PROSITE	PS50004. C2. 1 hit. PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 2 hits. PS50003. PH_DOMAIN. 1 hit. PS50007. PIPLC_X_DOMAIN. 1 hit. PS50008. PIPLC_Y_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

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Entry information

Entry name

PLCD1_MOUSE

Accession

Primary (citable) accession number: Q8R3B1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	June 1, 2002
Last modified:	October 13, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents