Reviewed,
UniProtKB/Swiss-Prot Q8R3B1 (PLCD1_MOUSE)
Last modified
October 13, 2009.
Version 77.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-1 EC=3.1.4.11 Alternative name(s): Phosphoinositide phospholipase C Phospholipase C-delta-1 Short name=PLC-delta-1 PLC-III | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 756 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Essential for trophoblast and placental development. Ref.2 |
| Catalytic activity | 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol. |
| Cofactor | Binds 3 calcium ions per subunit. Two of the calcium ions are bound to the C2 domain By similarity. |
| Disruption phenotype | Mice lacking Plcd1 and Plcd3 die at embryonic day 11.5 (E11.5) to E13.5 and exhibit severe disruption of the normal labyrinth architecture in the placenta and decreased placental vascularization, as well as abnormal proliferation and apoptosis of trophoblasts in the labyrinth area. Furthermore, Plcd1 and Plcd3 double-knockout embryos supplied with a normal placenta by the tetraploid aggregation method survive beyond E14.5, clearly indicating that the embryonic lethality is caused by a defect in trophoblasts. Ref.2 |
| Sequence similarities | Contains 1 C2 domain. Contains 2 EF-hand domains. Contains 1 PH domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Lipid degradation |
| Domain | Repeat |
| Ligand | Calcium Metal-binding |
| Molecular function | Hydrolase Transducer |
| Gene Ontology (GO) | |
| Biological process | angiogenesis Ref.2 Inferred from genetic interaction. Source: MGI intracellular signaling cascadeInferred from electronic annotation. Source: InterPro labyrinthine layer blood vessel development Ref.2Inferred from genetic interaction. Source: MGI lipid catabolic processInferred from electronic annotation. Source: UniProtKB-KW regulation of cell proliferation Ref.2Inferred from genetic interaction. Source: MGI |
| Cellular component | cytosol Inferred from direct assay. Source: MGI |
| Molecular function | calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoinositide phospholipase C activityInferred from electronic annotation. Source: EC signal transducer activityInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 756 | 756 | 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-1 | PRO_0000088505 | |||||
Regions | |||||||||
| Domain | 21 – 130 | 110 | PH | ||||||
| Domain | 140 – 175 | 36 | EF-hand 1 | ||||||
| Domain | 176 – 211 | 36 | EF-hand 2 | ||||||
| Domain | 296 – 440 | 145 | PI-PLC X-box | ||||||
| Domain | 492 – 609 | 118 | PI-PLC Y-box | ||||||
| Domain | 630 – 720 | 91 | C2 | ||||||
| Calcium binding | 153 – 164 | 12 | 1 Potential | ||||||
| Calcium binding | 189 – 200 | 12 | 2 Potential | ||||||
| Region | 30 – 57 | 28 | Substrate binding By similarity | ||||||
Sites | |||||||||
| Active site | 311 | 1 | By similarity | ||||||
| Active site | 356 | 1 | By similarity | ||||||
| Metal binding | 312 | 1 | Calcium 1; catalytic By similarity | ||||||
| Metal binding | 341 | 1 | Calcium 1; catalytic By similarity | ||||||
| Metal binding | 343 | 1 | Calcium 1; catalytic By similarity | ||||||
| Metal binding | 390 | 1 | Calcium 1; catalytic By similarity | ||||||
| Metal binding | 651 | 1 | Calcium 2; via carbonyl oxygen By similarity | ||||||
| Metal binding | 653 | 1 | Calcium 2 By similarity | ||||||
| Metal binding | 677 | 1 | Calcium 2 By similarity | ||||||
| Metal binding | 706 | 1 | Calcium 3 By similarity | ||||||
| Metal binding | 707 | 1 | Calcium 3; via carbonyl oxygen By similarity | ||||||
| Metal binding | 708 | 1 | Calcium 3 By similarity | ||||||
| Binding site | 438 | 1 | Substrate By similarity | ||||||
| Binding site | 440 | 1 | Substrate By similarity | ||||||
| Binding site | 522 | 1 | Substrate By similarity | ||||||
| Binding site | 549 | 1 | Substrate By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [2] | "Phospholipase C-delta1 and -delta3 are essential in the trophoblast for placental development." Nakamura Y., Hamada Y., Fujiwara T., Enomoto H., Hiroe T., Tanaka S., Nose M., Nakahara M., Yoshida N., Takenawa T., Fukami K. Mol. Cell. Biol. 25:10979-10988(2005) [PubMed: 16314520] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE. |
Cross-references
Sequence databases | |
|---|---|
| BC025798 mRNA. Translation: AAH25798.1. | |
| IPI | IPI00321515. |
| UniGene | Mm.23963 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1MAI based on UniProtKB P10688. |
| SMR | Q8R3B1. Positions 12-129, 158-756. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q8R3B1. |
PTM databases | |
| PhosphoSite | Q8R3B1. |
Proteomic databases | |
| PRIDE | Q8R3B1. |
Genome annotation databases | |
| Ensembl | ENSMUST00000010804; ENSMUSP00000010804; ENSMUSG00000010660; Mus musculus. [Genome view] |
Organism-specific databases | |
| MGI | MGI:97614. Plcd1. |
Phylogenomic databases | |
| HOGENOM | Q8R3B1. |
| HOVERGEN | Q8R3B1. |
Enzyme and pathway databases | |
| BRENDA | 3.1.4.11. 244. |
Gene expression databases | |
| ArrayExpress | Q8R3B1. |
| Bgee | Q8R3B1. |
| CleanEx | MM_PLCD1. |
| Genevestigator | Q8R3B1. |
| GermOnline | ENSMUSG00000010660. Mus musculus. |
Family and domain databases | |
| InterPro | IPR000008. C2_Ca-dep. IPR018029. C2_membr_targeting. IPR011992. EF-Hand_type. IPR018247. EF_HAND_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca_bd. IPR011993. PH_type. IPR015359. Phospholipase_C_EF-hand-like. IPR001192. Phospholipase_C_Pinositol-sp_C. IPR000909. Phospholipase_C_Pinositol-sp_X. IPR001711. Phospholipase_C_Pinositol-sp_Y. IPR017946. PLC-like_Pdiesterase_TIM-brl. IPR001849. Pleckstrin_homology. [Graphical view] |
| Gene3D | G3DSA:1.10.238.10. EF-Hand_type. 2 hits. G3DSA:2.30.29.30. PH_type. 1 hit. G3DSA:3.20.20.190. PLC-like_Pdiesterase_TIM-brl. 1 hit. |
| Pfam | PF00168. C2. 1 hit. PF09279. efhand_like. 1 hit. PF00169. PH. 1 hit. PF00388. PI-PLC-X. 1 hit. PF00387. PI-PLC-Y. 1 hit. [Graphical view] |
| PRINTS | PR00390. PHPHLIPASEC. |
| ProDom | PD001202. PI_PLC_Y. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00239. C2. 1 hit. SM00054. EFh. 2 hits. SM00233. PH. 1 hit. SM00148. PLCXc. 1 hit. SM00149. PLCYc. 1 hit. [Graphical view] |
| PROSITE | PS50004. C2. 1 hit. PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 2 hits. PS50003. PH_DOMAIN. 1 hit. PS50007. PIPLC_X_DOMAIN. 1 hit. PS50008. PIPLC_Y_DOMAIN. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| SOURCE | Search... |
Entry information
| Entry name | PLCD1_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q8R3B1 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


