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Q8R3B1

- PLCD1_MOUSE

UniProt

Q8R3B1 - PLCD1_MOUSE

Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1

Gene

Plcd1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Essential for trophoblast and placental development.1 Publication

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

    Cofactori

    Binds 3 calcium ions per subunit. Two of the calcium ions are bound to the C2 domain By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei311 – 3111PROSITE-ProRule annotation
    Metal bindingi312 – 3121Calcium 1; catalyticBy similarity
    Metal bindingi341 – 3411Calcium 1; catalyticBy similarity
    Metal bindingi343 – 3431Calcium 1; catalyticBy similarity
    Active sitei356 – 3561PROSITE-ProRule annotation
    Metal bindingi390 – 3901Calcium 1; catalyticBy similarity
    Binding sitei438 – 4381SubstrateBy similarity
    Binding sitei440 – 4401SubstrateBy similarity
    Binding sitei522 – 5221SubstrateBy similarity
    Binding sitei549 – 5491SubstrateBy similarity
    Metal bindingi651 – 6511Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi653 – 6531Calcium 2By similarity
    Metal bindingi677 – 6771Calcium 2By similarity
    Metal bindingi706 – 7061Calcium 3By similarity
    Metal bindingi707 – 7071Calcium 3; via carbonyl oxygenBy similarity
    Metal bindingi708 – 7081Calcium 3By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi153 – 164121PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi189 – 200122PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. phosphatidic acid binding Source: MGI
    3. phosphatidylinositol phosphate binding Source: MGI
    4. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
    5. phosphatidylserine binding Source: MGI
    6. phospholipase C activity Source: MGI
    7. protein binding Source: MGI
    8. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. angiogenesis Source: MGI
    2. intracellular signal transduction Source: InterPro
    3. labyrinthine layer blood vessel development Source: MGI
    4. lipid catabolic process Source: UniProtKB-KW
    5. regulation of cell proliferation Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Transducer

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    BRENDAi3.1.4.11. 3474.
    ReactomeiREACT_196473. Synthesis of IP3 and IP4 in the cytosol.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 (EC:3.1.4.11)
    Alternative name(s):
    Phosphoinositide phospholipase C-delta-1
    Phospholipase C-delta-1
    Short name:
    PLC-delta-1
    Gene namesi
    Name:Plcd1
    Synonyms:Plcd
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:97614. Plcd1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. cytosol Source: MGI

    Pathology & Biotechi

    Disruption phenotypei

    Mice lacking Plcd1 and Plcd3 die between 11.5 and 13.5 dpc. They exhibit severe disruption of the normal labyrinth architecture in the placenta and decreased placental vascularization, as well as abnormal proliferation and apoptosis of trophoblasts in the labyrinth area. Furthermore, Plcd1 and Plcd3 double knockout embryos supplied with a normal placenta by the tetraploid aggregation method survive beyond 14.5 dpc, indicating that the embryonic lethality is caused by a defect in trophoblasts.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 7567561-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1PRO_0000088505Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi191 – 1911O-linked (GlcNAc)By similarity
    Glycosylationi193 – 1931O-linked (GlcNAc)By similarity

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ8R3B1.
    PaxDbiQ8R3B1.
    PRIDEiQ8R3B1.

    PTM databases

    PhosphoSiteiQ8R3B1.

    Expressioni

    Tissue specificityi

    Highly expressed in brain, heart, lung, epididymis and testis. Detected at lower levels in kidney and skeletal muscle.1 Publication

    Gene expression databases

    BgeeiQ8R3B1.
    CleanExiMM_PLCD1.
    GenevestigatoriQ8R3B1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8R3B1.
    SMRiQ8R3B1. Positions 12-129, 158-756.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 130110PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini140 – 17536EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini176 – 21136EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini296 – 440145PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini492 – 609118PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini630 – 72091C2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni30 – 5728Substrate bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 2 EF-hand domains.PROSITE-ProRule annotation
    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG149692.
    GeneTreeiENSGT00740000114979.
    HOGENOMiHOG000006871.
    HOVERGENiHBG053610.
    InParanoidiQ9Z1B4.
    KOiK05857.
    OMAiTSGQAFY.
    OrthoDBiEOG7V49XT.
    TreeFamiTF313216.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    2.30.29.30. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR001192. PI-PLC_fam.
    IPR028391. PLC-delta1.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF80. PTHR10336:SF80. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00169. PH. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view]
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00054. EFh. 2 hits.
    SM00233. PH. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS50003. PH_DOMAIN. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8R3B1-1 [UniParc]FASTAAdd to Basket

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    MDSGRDFLTL HGLQDDPDLQ ALLKGSQLLK VKSSSWRRER FYKLQEDCKT    50
    IWQESRKVMR SPESQLFSIE DIQEVRMGHR TEGLEKFARD IPEDRCFSIV 100
    FKDQRNTLDL IAPSPADVQH WVQGLRKIID RSGSMDQRQK LQHWIHSCLR 150
    KADKNKDNKM NFKEVKDFLK ELNVQVDDSY ARKIFRECDH SQTDSLEDEE 200
    IETFYRMLTQ RAEIDRAFAE AAGSAETLSV EKLVTFLQHQ QREEEAGPAL 250
    ALSLIERYEP SETAKAQRQM TKDGFLMYLL SADGNAFSLA HRRVYQDMNQ 300
    PLSHYLVSSS HNTYLLEDQL TGPSSTEAYI RALCKGCRCL ELDCWDGPNQ 350
    EPIIYHGYTF TSKILFCDVL RAIRDYAFKA SPYPVILSLE NHCSLEQQRV 400
    MAHHLRAILG PMLLDQPLDG VTTSLPSPEQ LKEKILLKGK KLGGLLPAGG 450
    ENGPEATDVS DEDEAAEMED EAVRSQVQHK PKEDKLKLVP ELSDMVIYCK 500
    SVHFGGFSSP STSGQAFYEM ASFSESRALR LLQESGNSFV RHNVGHLSRI 550
    YPAGWRTDSS NYSPVEMWNG GCQIVALNFQ TPGPEMDVYL GCFQDNGGCG 600
    YVLKPAFLRD PDTTFNSRAL TQGPWWAPKK LRVWIISGQQ LPKVNKNKNS 650
    IVDPKVIVEI HGVGQDVASR QTAVITNNGF NPRWDTEFEF VVAVPDLALV 700
    RFMVEDYDSS SKNDFIGQST IPWNSLKQGY RHVHLLSKNG DLHPSATLFV 750
    KISIQD 756
    Length:756
    Mass (Da):85,873
    Last modified:July 27, 2011 - v2
    Checksum:i71F17810F9B0B938
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti212 – 2121A → V in AAH25798. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF133125 mRNA. Translation: AAD32616.1.
    U85711 mRNA. Translation: AAD00570.1.
    AK028749 mRNA. Translation: BAC26096.1.
    AK082890 mRNA. Translation: BAC38671.1.
    CH466587 Genomic DNA. Translation: EDL09242.1.
    BC025798 mRNA. Translation: AAH25798.1.
    CCDSiCCDS23607.1.
    RefSeqiNP_062650.1. NM_019676.3.
    UniGeneiMm.23963.

    Genome annotation databases

    EnsembliENSMUST00000010804; ENSMUSP00000010804; ENSMUSG00000010660.
    GeneIDi18799.
    KEGGimmu:18799.
    UCSCiuc009saf.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF133125 mRNA. Translation: AAD32616.1 .
    U85711 mRNA. Translation: AAD00570.1 .
    AK028749 mRNA. Translation: BAC26096.1 .
    AK082890 mRNA. Translation: BAC38671.1 .
    CH466587 Genomic DNA. Translation: EDL09242.1 .
    BC025798 mRNA. Translation: AAH25798.1 .
    CCDSi CCDS23607.1.
    RefSeqi NP_062650.1. NM_019676.3.
    UniGenei Mm.23963.

    3D structure databases

    ProteinModelPortali Q8R3B1.
    SMRi Q8R3B1. Positions 12-129, 158-756.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8R3B1.

    Proteomic databases

    MaxQBi Q8R3B1.
    PaxDbi Q8R3B1.
    PRIDEi Q8R3B1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000010804 ; ENSMUSP00000010804 ; ENSMUSG00000010660 .
    GeneIDi 18799.
    KEGGi mmu:18799.
    UCSCi uc009saf.1. mouse.

    Organism-specific databases

    CTDi 5333.
    MGIi MGI:97614. Plcd1.

    Phylogenomic databases

    eggNOGi NOG149692.
    GeneTreei ENSGT00740000114979.
    HOGENOMi HOG000006871.
    HOVERGENi HBG053610.
    InParanoidi Q9Z1B4.
    KOi K05857.
    OMAi TSGQAFY.
    OrthoDBi EOG7V49XT.
    TreeFami TF313216.

    Enzyme and pathway databases

    BRENDAi 3.1.4.11. 3474.
    Reactomei REACT_196473. Synthesis of IP3 and IP4 in the cytosol.

    Miscellaneous databases

    NextBioi 295096.
    PROi Q8R3B1.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8R3B1.
    CleanExi MM_PLCD1.
    Genevestigatori Q8R3B1.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    2.30.29.30. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR001192. PI-PLC_fam.
    IPR028391. PLC-delta1.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF80. PTHR10336:SF80. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00169. PH. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view ]
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00054. EFh. 2 hits.
    SM00233. PH. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS50003. PH_DOMAIN. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression analysis of a mouse phospholipase C-delta1."
      Lee W.K., Kim J.K., Seo M.S., Cha J.H., Lee K.J., Rha H.K., Min D.S., Jo Y.H., Lee K.H.
      Biochem. Biophys. Res. Commun. 261:393-399(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: BALB/c.
      Tissue: Brain.
    2. Wu K., Bai J., Marks D.L., Pagano R.E.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Swiss.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryo and Skin.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "Phospholipase C-delta1 and -delta3 are essential in the trophoblast for placental development."
      Nakamura Y., Hamada Y., Fujiwara T., Enomoto H., Hiroe T., Tanaka S., Nose M., Nakahara M., Yoshida N., Takenawa T., Fukami K.
      Mol. Cell. Biol. 25:10979-10988(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiPLCD1_MOUSE
    AccessioniPrimary (citable) accession number: Q8R3B1
    Secondary accession number(s): Q9Z1B4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2002
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 126 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3