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Protein

Immunoglobulin superfamily member 8

Gene

Igsf8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain.2 Publications

GO - Biological processi

  • regulation of cell motility Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Immunoglobulin superfamily member 8
Short name:
IgSF8
Alternative name(s):
CD81 partner 3
Glu-Trp-Ile EWI motif-containing protein 2
Short name:
EWI-2
Keratinocyte-associated transmembrane protein 4
Short name:
KCT-4
Prostaglandin regulatory-like protein
Short name:
PGRL
CD_antigen: CD316
Gene namesi
Name:Igsf8
Synonyms:Ewi2, Kct4, Pgrl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2154090. Igsf8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini26 – 577552ExtracellularSequence analysisAdd
BLAST
Transmembranei578 – 59821HelicalSequence analysisAdd
BLAST
Topological domaini599 – 61113CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • axon Source: MGI
  • extracellular exosome Source: MGI
  • integral component of membrane Source: UniProtKB
  • membrane Source: MGI
  • myelin sheath Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
  • synapse Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525By similarityAdd
BLAST
Chaini26 – 611586Immunoglobulin superfamily member 8PRO_0000226248Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi47 ↔ 125PROSITE-ProRule annotation
Glycosylationi48 – 481N-linked (GlcNAc...)Sequence analysis
Glycosylationi137 – 1371N-linked (GlcNAc...)Sequence analysis
Disulfide bondi184 ↔ 268PROSITE-ProRule annotation
Disulfide bondi324 ↔ 404PROSITE-ProRule annotation
Glycosylationi325 – 3251N-linked (GlcNAc...)Sequence analysis
Disulfide bondi460 ↔ 542PROSITE-ProRule annotation
Modified residuei516 – 5161PhosphoserineBy similarity
Lipidationi602 – 6021S-palmitoyl cysteine1 Publication
Lipidationi603 – 6031S-palmitoyl cysteine1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

EPDiQ8R366.
MaxQBiQ8R366.
PaxDbiQ8R366.
PeptideAtlasiQ8R366.
PRIDEiQ8R366.

PTM databases

iPTMnetiQ8R366.
PhosphoSiteiQ8R366.
SwissPalmiQ8R366.

Expressioni

Tissue specificityi

Expressed in lymphocytes as well as in many tissues with higher expression in brain. Detected in all regions of the brain with weak expression in the pituitary. Expressed selectively by neurons but not by glial cells.1 Publication

Developmental stagei

Expressed at E9.5 in the ventral domain of the neural tube, with dorsal expression apparent at E10.5, as well as in the dorsal bud of the pancreas. Detected at E11.5 within the ventricular zone of the neural tube in the hindbrain, diencephalon and telencephalon; also detected in the epithelial lining of the bronchi and esophagus. At 12.5, expressed in the bronchi, in the atrial and ventricular myocardium, the dorsal root ganglia and the epithelial lining of the rectum and bladder. At E15.5, detected in the developing choroid plexus epithelium, neural retina, olfactory epithelium and developing vomeronasal organ.1 Publication

Inductioni

Induced by neurite outgrowth in neuroblastoma cell lines Neuro-2a.1 Publication

Gene expression databases

BgeeiQ8R366.
CleanExiMM_IGSF8.

Interactioni

Subunit structurei

Interacts directly with CD82 and CD9/tetraspanin-29. Also interacts with integrin alpha-3/beta-1 and integrin alpha-4/beta-1 (By similarity). Interacts with CD81/tetraspanin-28.By similarity1 Publication

Protein-protein interaction databases

BioGridi228282. 2 interactions.
IntActiQ8R366. 1 interaction.
MINTiMINT-4118899.
STRINGi10090.ENSMUSP00000041232.

Structurei

3D structure databases

ProteinModelPortaliQ8R366.
SMRiQ8R366. Positions 30-203, 309-546.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 143118Ig-like C2-type 1Add
BLAST
Domaini160 – 284125Ig-like C2-type 2Add
BLAST
Domaini301 – 422122Ig-like C2-type 3Add
BLAST
Domaini429 – 554126Ig-like C2-type 4Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi272 – 2743EWI motif

Domaini

The Ig-like C2-type domains 3 and 4 are required for interaction with CD81.
The short cytoplasmic domain is very basic, interacts with membrane PIPs, and mediates plasma membrane localization.

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHZZ. Eukaryota.
ENOG410XSYU. LUCA.
HOGENOMiHOG000113020.
HOVERGENiHBG073047.
InParanoidiQ8R366.
KOiK06730.
PhylomeDBiQ8R366.
TreeFamiTF332702.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 4 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 4 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R366-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVPSPTPLS SLLLLLLILG TRCYARQVHV PRGPLYRVAG TAVSISCNVS
60 70 80 90 100
DYEGPAQQDF EWFMYRPEAP ATSLGIVSTK DSQFSYAVFG PRVASGDLQV
110 120 130 140 150
QRLKGDSVVL KIARLQAQDS GFYECYTPST DTQYLGNYSA KVELRVLPDE
160 170 180 190 200
LQVSAAPPGP RGRQAATSPS RLTVHEGQEL ALGCLAQTKT KKHTHLSVSF
210 220 230 240 250
GRAIPEAPVG RATLQEVVGL RSDMAVEAGA PYAERLASGE LRLSKEGTDR
260 270 280 290 300
YRMVVGGAQA GDSGTYHCTA AEWIQDPDGS WVQVAEKRAV LAHVDVQTLS
310 320 330 340 350
SQLAVTVGPG ERRIGPGEPL ELLCNVSGAL PPPGRHAAYS VGWEMAPAGA
360 370 380 390 400
PGPGRLVAQL DTEGIGSLGP GYEDRHIAME KVASRTYRLR LEAARPADAG
410 420 430 440 450
TYRCLAKAYV RGSGTRLREA ASARSRPLPV HVREEGVVLE AVAWLAGGTV
460 470 480 490 500
YRGETASLLC NISVRGGPPG LRLAASWWVE RPEEGELSSG PAQLVGGVGQ
510 520 530 540 550
DGVAELGVRP GGGPVSVELV GPRSHRLRLH GLGPEDEGIY HCAPSAWVQH
560 570 580 590 600
ADYSWYQAGS ARSGPVTVYP YTHAVDTLFV PLLVGTGVAL VTGASVLATI
610
TCCFMKRMRK R
Length:611
Mass (Da):65,011
Last modified:March 7, 2006 - v2
Checksum:iB5581BE0DF01565A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti20 – 201G → E in BAE26989 (PubMed:16141072).Curated
Sequence conflicti20 – 201G → E in BAE40298 (PubMed:16141072).Curated
Sequence conflicti37 – 371R → Q in BAE26904 (PubMed:16141072).Curated
Sequence conflicti221 – 2211R → H in BAE26904 (PubMed:16141072).Curated
Sequence conflicti221 – 2211R → H in AAH48387 (PubMed:15489334).Curated
Sequence conflicti316 – 3161P → A in BAE26989 (PubMed:16141072).Curated
Sequence conflicti316 – 3161P → A in BAE40298 (PubMed:16141072).Curated
Sequence conflicti319 – 3191P → H in BAE26904 (PubMed:16141072).Curated
Sequence conflicti470 – 4712GL → RV in AAL02217 (PubMed:11673522).Curated
Sequence conflicti489 – 4891S → T (PubMed:11673522).Curated
Sequence conflicti489 – 4891S → T in BAE26904 (PubMed:16141072).Curated
Sequence conflicti489 – 4891S → T in AAH48387 (PubMed:15489334).Curated
Sequence conflicti547 – 5471W → S in BAE26904 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF411055 mRNA. Translation: AAL02217.1.
AF439263 mRNA. Translation: AAN63626.1.
AK146104 mRNA. Translation: BAE26904.1.
AK146221 mRNA. Translation: BAE26989.1.
AK168363 mRNA. Translation: BAE40298.1.
BC026464 mRNA. Translation: AAH26464.1.
BC026633 mRNA. Translation: AAH26633.1.
BC048387 mRNA. Translation: AAH48387.1.
CCDSiCCDS15511.1.
RefSeqiNP_536344.1. NM_080419.2.
UniGeneiMm.271717.
Mm.487654.

Genome annotation databases

GeneIDi140559.
KEGGimmu:140559.
UCSCiuc007dqe.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF411055 mRNA. Translation: AAL02217.1.
AF439263 mRNA. Translation: AAN63626.1.
AK146104 mRNA. Translation: BAE26904.1.
AK146221 mRNA. Translation: BAE26989.1.
AK168363 mRNA. Translation: BAE40298.1.
BC026464 mRNA. Translation: AAH26464.1.
BC026633 mRNA. Translation: AAH26633.1.
BC048387 mRNA. Translation: AAH48387.1.
CCDSiCCDS15511.1.
RefSeqiNP_536344.1. NM_080419.2.
UniGeneiMm.271717.
Mm.487654.

3D structure databases

ProteinModelPortaliQ8R366.
SMRiQ8R366. Positions 30-203, 309-546.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228282. 2 interactions.
IntActiQ8R366. 1 interaction.
MINTiMINT-4118899.
STRINGi10090.ENSMUSP00000041232.

PTM databases

iPTMnetiQ8R366.
PhosphoSiteiQ8R366.
SwissPalmiQ8R366.

Proteomic databases

EPDiQ8R366.
MaxQBiQ8R366.
PaxDbiQ8R366.
PeptideAtlasiQ8R366.
PRIDEiQ8R366.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi140559.
KEGGimmu:140559.
UCSCiuc007dqe.1. mouse.

Organism-specific databases

CTDi93185.
MGIiMGI:2154090. Igsf8.

Phylogenomic databases

eggNOGiENOG410IHZZ. Eukaryota.
ENOG410XSYU. LUCA.
HOGENOMiHOG000113020.
HOVERGENiHBG073047.
InParanoidiQ8R366.
KOiK06730.
PhylomeDBiQ8R366.
TreeFamiTF332702.

Miscellaneous databases

ChiTaRSiIgsf8. mouse.
PROiQ8R366.
SOURCEiSearch...

Gene expression databases

BgeeiQ8R366.
CleanExiMM_IGSF8.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 4 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 4 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "PGRL is a major CD81-associated protein on lymphocytes and distinguishes a new family of cell surface proteins."
    Clark K.L., Zeng Z., Langford A.L., Bowen S.M., Todd S.C.
    J. Immunol. 167:5115-5121(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH CD81, IDENTIFICATION BY MASS SPECTROMETRY, DOMAIN.
  2. "Genomic organization and embryonic expression of Igsf8, an immunoglobulin superfamily member implicated in development of the nervous system and organ epithelia."
    Murdoch J.N., Doudney K., Gerrelli D., Wortham N., Paternotte C., Stanier P., Copp A.J.
    Mol. Cell. Neurosci. 22:62-74(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: BALB/cJ.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and FVB/N.
    Tissue: Colon and Mammary tumor.
  5. "Neuronal expression of keratinocyte-associated transmembrane protein-4, KCT-4, in mouse brain and its up-regulation by neurite outgrowth of Neuro-2a cells."
    Yamada O., Tamura K., Yagihara H., Isotani M., Washizu T., Bonkobara M.
    Neurosci. Lett. 392:226-230(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Kidney, Spleen and Testis.
  7. "Differential functions of phospholipid binding and palmitoylation of tumour suppressor EWI2/PGRL."
    He B., Zhang Y.H., Richardson M.M., Zhang J.S., Rubinstein E., Zhang X.A.
    Biochem. J. 437:399-411(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION AT CYS-602 AND CYS-603, DOMAIN, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiIGSF8_MOUSE
AccessioniPrimary (citable) accession number: Q8R366
Secondary accession number(s): Q8R0L7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: March 7, 2006
Last modified: July 6, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.