Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquilin-1

Gene

Ubqln1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the regulation of different protein degradation mechanisms and pathways including ubiquitin-proteasome system (UPS), autophagy and endoplasmic reticulum-associated protein degradation (ERAD) pathway. Mediates the proteasomal targeting of misfolded or accumulated proteins for degradation by binding (via UBA domain) to their polyubiquitin chains and by interacting (via ubiquitin-like domain) with the subunits of the proteasome. Plays a role in the ERAD pathway via its interaction with ER-localized proteins UBXN4, VCP and HERPUD1 and may form a link between the polyubiquitinated ERAD substrates and the proteasome. Plays a role in unfolded protein response (UPR) by attenuating the induction of UPR-inducible genes, DDTI3/CHOP, HSPA5 and PDIA2 during ER stress. Involved in the regulation of macroautophagy and autophagosome formation; required for maturation of autophagy-related protein LC3 from the cytosolic form LC3-I to the membrane-bound form LC3-II and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion. Negatively regulates the TICAM1/TRIF-dependent toll-like receptor signaling pathway by decreasing the abundance of TICAM1 via the autophagic pathway. Plays a key role in the regulation of the levels of PSEN1 by targeting its accumulation to aggresomes which may then be removed from cells by autophagocytosis. Promotes the ubiquitination and lysosomal degradation of ORAI1, consequently downregulating the ORAI1-mediated Ca2+ mobilization. Suppresses the maturation and proteasomal degradation of amyloid beta A4 protein (A4) by stimulating the lysine 63 (K63)-linked polyubiquitination. Delays the maturation of A4 by sequestering it in the Golgi apparatus and preventing its transport to the cell surface for subsequent processing (By similarity). Links CD47 to the cytoskeleton (PubMed:10549293).By similarity1 Publication

GO - Molecular functioni

  • identical protein binding Source: MGI
  • intermediate filament binding Source: UniProtKB
  • kinase binding Source: UniProtKB
  • polyubiquitin modification-dependent protein binding Source: UniProtKB
  • receptor binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processAutophagy

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquilin-1
Alternative name(s):
Protein linking IAP with cytoskeleton 1
Short name:
PLIC-1
Gene namesi
Name:Ubqln1
Synonyms:Plic1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1860276. Ubqln1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002110092 – 582Ubiquilin-1Add BLAST581

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Post-translational modificationi

Degraded during both macroautophagy and during chaperone-mediated autophagy (CMA).By similarity
Phosphorylated.By similarity
Ubiquitinated.By similarity

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

EPDiQ8R317.
PaxDbiQ8R317.
PeptideAtlasiQ8R317.
PRIDEiQ8R317.

2D gel databases

REPRODUCTION-2DPAGEiQ8R317.

PTM databases

iPTMnetiQ8R317.
PhosphoSitePlusiQ8R317.

Expressioni

Tissue specificityi

Highly expressed in heart, brain, liver, smooth muscle and kidney.1 Publication

Gene expression databases

BgeeiENSMUSG00000005312.
CleanExiMM_UBQLN1.
GenevisibleiQ8R317. MM.

Interactioni

Subunit structurei

Monomer and homodimer. Heterodimer with UBQLN2 (By similarity). Binds CD47 (PubMed:10549293). Binds NBL1, GABRA1, GABRA2, GABRA3, GABRA6, GABRB1, GABRB2 and GABRB3. Binds UBE3A, BTRC, P4HB and MTOR. Interacts with the proteasome 19S subunit. Interacts (via ubiquitin-like domain) with TREX1; the interaction is direct and may control TREX1 subcellular location. Forms a complex with UBXN4 and VCP. Interacts (via UBA domain) with UBQLN4 (via ubiquitin-like domain). Found in a complex with UBQLN2 and MAP1LC3A/B/C. The monomeric form interacts with PSEN1 and PSEN2. Interacts with ORAI1. Interacts (via UBA domain) with TICAM1. Interacts with EPS15. Interacts (via UBA domain) with UBA52 and (via ubiquitin-like domain) with PSMD3 and PSMD4. Interacts with HERPUD1. Interacts with MAP1LC3A/B/C in the presence of UBQLN4. Interacts (via ubiquitin-like domain) with EPS15 (via UIM domains) and both the ubiquitinated and non-ubiquitinated forms can interact with EPS15. Interacts (via ubiquitin-like domain) with EPS15L1, HGS (via UIM domain) and STAM2 (via UIM domain) (By similarity).By similarity1 Publication

GO - Molecular functioni

  • identical protein binding Source: MGI
  • intermediate filament binding Source: UniProtKB
  • kinase binding Source: UniProtKB
  • polyubiquitin modification-dependent protein binding Source: UniProtKB
  • receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi207807. 5 interactors.
IntActiQ8R317. 4 interactors.
MINTiMINT-4139320.
STRINGi10090.ENSMUSP00000050191.

Structurei

3D structure databases

ProteinModelPortaliQ8R317.
SMRiQ8R317.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 102Ubiquitin-likePROSITE-ProRule annotationAdd BLAST75
Domaini173 – 201STI1 1Sequence analysisAdd BLAST29
Domaini203 – 242STI1 2Sequence analysisAdd BLAST40
Domaini381 – 428STI1 3Sequence analysisAdd BLAST48
Domaini432 – 464STI1 4Sequence analysisAdd BLAST33
Domaini539 – 579UBAPROSITE-ProRule annotationAdd BLAST41

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni169 – 422Interaction with UBXN4By similarityAdd BLAST254

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi372 – 546Gln-richAdd BLAST175

Domaini

The UBA domain mediates binding to PSEN1 and PSEN2. It also binds ubiquitin with micromolar affinity, independently of polyubiquitin linkage type. Essential for its association with microtubule-associated protein 1 light chain 3 (MAP1LC3).By similarity
The ubiquitin-like domain mediates its association with the subunits of the proteasome.By similarity
Dimerization is dependent upon the central region of the protein containing the STI1 domains and is independent of its ubiquitin-like and UBA domains.By similarity

Phylogenomic databases

eggNOGiKOG0010. Eukaryota.
COG5272. LUCA.
GeneTreeiENSGT00390000005720.
HOVERGENiHBG064537.
InParanoidiQ8R317.
KOiK04523.
OMAiIQQMLQA.
OrthoDBiEOG091G08WB.
PhylomeDBiQ8R317.
TreeFamiTF314412.

Family and domain databases

InterProiView protein in InterPro
IPR006636. STI1_HS-bd.
IPR015940. UBA.
IPR009060. UBA-like.
IPR015496. Ubiquilin.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
IPR028799. UBQLN1.
PANTHERiPTHR10677. PTHR10677. 1 hit.
PTHR10677:SF29. PTHR10677:SF29. 1 hit.
PfamiView protein in Pfam
PF00627. UBA. 1 hit.
PF00240. ubiquitin. 1 hit.
SMARTiView protein in SMART
SM00727. STI1. 4 hits.
SM00165. UBA. 1 hit.
SM00213. UBQ. 1 hit.
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiView protein in PROSITE
PS50030. UBA. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R317-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAESAESGGP PGAQDSAADG GPAEPKIMKV TVKTPKEKEE FAVPENSSVQ
60 70 80 90 100
QFKEEISKRF KSHIDQLVLI FAGKILKDQD TLSQHGIHDG LTVHLVIKTQ
110 120 130 140 150
NRPQDNSAQQ TNAPGSTVTS SPAPDSNPTS GSAANSSFGV GGLGGLAGLS
160 170 180 190 200
SLGLNTTNFS ELQSQMQRQL LSNPEMMVQI MENPFVQSML SNPDLMRQLI
210 220 230 240 250
MANPQMQQLI QRNPEISHML NNPDIMRQTL ELARNPAMMQ EMMRNQDRAL
260 270 280 290 300
SNLESIPGGY NALRRMYTDI QEPMLNAAQE QFGGNPFASL VSSSSSAEGT
310 320 330 340 350
QPSRTENRDP LPNPWAPQTS QSSPASGTTG STTNTMSTSG GTATSTPAGQ
360 370 380 390 400
STSGPSLVPG AGASMFNTPG MQSLLQQITE NPQLMQNMLS APYMRSMLQS
410 420 430 440 450
LSQNPDLAAQ MMLNNPLFAG NPQLQEQMRQ QLPTFLQQMQ NPDTLSAMSN
460 470 480 490 500
PRAMQALLQI QQGLQTLATE APGLIPGFTP GLAAGNSGGS SGTNAPSTAP
510 520 530 540 550
SEDTNPQGGT AEPGHQQFIQ QMLQALAGVN PQLQSPEVRF QQQLEQLSAM
560 570 580
GFLNREANLQ ALIATGGDIN AAIERLLGSQ PS
Length:582
Mass (Da):61,976
Last modified:June 1, 2002 - v1
Checksum:iDF0CF98794CC3A04
GO
Isoform 2 (identifier: Q8R317-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     410-437: Missing.

Show »
Length:554
Mass (Da):58,678
Checksum:iF9255C9985A4C029
GO

Sequence cautioni

The sequence AAF01365 differs from that shown. Several sequencing errors.Curated
The sequence AAH51098 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAC33666 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti175E → G in BAC33365 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009788410 – 437Missing in isoform 2. 2 PublicationsAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF177345 mRNA. Translation: AAF01365.1. Sequence problems.
AK048534 mRNA. Translation: BAC33365.1.
AK049298 mRNA. Translation: BAC33666.1. Different initiation.
AK004183 mRNA. Translation: BAB23211.1.
BC010213 mRNA. Translation: AAH10213.1.
BC026847 mRNA. Translation: AAH26847.1.
BC027375 mRNA. Translation: AAH27375.1.
BC028857 mRNA. Translation: AAH28857.1.
BC051098 mRNA. Translation: AAH51098.1. Different initiation.
CCDSiCCDS26569.1. [Q8R317-2]
CCDS49281.1. [Q8R317-1]
RefSeqiNP_081118.4. NM_026842.4. [Q8R317-1]
NP_689420.1. NM_152234.2. [Q8R317-2]
UniGeneiMm.182053.

Genome annotation databases

EnsembliENSMUST00000058735; ENSMUSP00000050191; ENSMUSG00000005312. [Q8R317-1]
ENSMUST00000076454; ENSMUSP00000075782; ENSMUSG00000005312. [Q8R317-2]
GeneIDi56085.
KEGGimmu:56085.
UCSCiuc007qtl.2. mouse. [Q8R317-2]
uc007qtm.2. mouse. [Q8R317-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUBQL1_MOUSE
AccessioniPrimary (citable) accession number: Q8R317
Secondary accession number(s): Q80V10
, Q8C7T4, Q8C835, Q8K141, Q91VI8, Q9D0Z0, Q9QZM1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: June 1, 2002
Last modified: September 27, 2017
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot