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Protein

Vacuolar protein sorting-associated protein 18 homolog

Gene

Vps18

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (By similarity). Required for fusion of endosomes and autophagosomes with lysosomes (PubMed:14517315, PubMed:22854957). Involved in dendrite development of Pukinje cells (PubMed:22699122).By similarity3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri853 – 94795RING-typeAdd
BLAST

GO - Molecular functioni

  • actin binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • protein binding, bridging Source: GO_Central
  • syntaxin binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 18 homolog
Gene namesi
Name:Vps18
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2443626. Vps18.

Subcellular locationi

  • Late endosome membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
  • Lysosome membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
  • Early endosome By similarity
  • Cytoplasmic vesicleautophagosome Curated
  • Cytoplasmic vesicleclathrin-coated vesicle Curated

  • Note: Cytoplasmic, peripheral membrane protein associated with early endosomes and late endosomes/lysosomes.

GO - Cellular componenti

  • actin filament Source: MGI
  • autophagosome Source: UniProtKB-SubCell
  • clathrin-coated vesicle Source: UniProtKB-SubCell
  • CORVET complex Source: UniProtKB
  • early endosome Source: MGI
  • HOPS complex Source: MGI
  • late endosome Source: MGI
  • late endosome membrane Source: UniProtKB-SubCell
  • lysosomal membrane Source: MGI
  • lysosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 973972Vacuolar protein sorting-associated protein 18 homologPRO_0000055907Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei3 – 31PhosphoserineBy similarity
Modified residuei11 – 111PhosphoserineBy similarity
Modified residuei13 – 131PhosphoserineBy similarity
Modified residuei362 – 3621N6-acetyllysineBy similarity
Modified residuei689 – 6891PhosphoserineBy similarity
Modified residuei912 – 9121PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8R307.
MaxQBiQ8R307.
PaxDbiQ8R307.
PeptideAtlasiQ8R307.
PRIDEiQ8R307.

PTM databases

iPTMnetiQ8R307.
PhosphoSiteiQ8R307.

Expressioni

Gene expression databases

BgeeiENSMUSG00000034216.
CleanExiMM_VPS18.
GenevisibleiQ8R307. MM.

Interactioni

Subunit structurei

Core component of at least two putative endosomal tethering complexes, the homotypic fusion and vacuole protein sorting (HOPS) complex and the class C core vacuaole/endosome tethering (CORVET) complex. Their common core is composed of the class C Vps proteins VPS11, VPS16, VPS18 and VPS33A, which in HOPS further associates with VPS39 and VPS41 and in CORVET with VPS8 and TGFBRAP1. Interacts with RAB5C (PubMed:25266290). Interacts with HOOK1 (PubMed:14668490). Interacts with STX7, MON1B (By similarity). Associates with adaptor protein complex 3 (AP-3) and clathrin:AP-3 complexes (PubMed:21411634).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
VIPAS39Q9H9C15EBI-2527788,EBI-749080From a different organism.

GO - Molecular functioni

  • actin binding Source: MGI
  • protein binding, bridging Source: GO_Central
  • syntaxin binding Source: MGI

Protein-protein interaction databases

BioGridi230740. 17 interactions.
IntActiQ8R307. 3 interactions.
STRINGi10090.ENSMUSP00000036915.

Structurei

3D structure databases

ProteinModelPortaliQ8R307.
SMRiQ8R307. Positions 284-399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati618 – 772155CHCRAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili454 – 48128Sequence analysisAdd
BLAST
Coiled coili802 – 84847Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the VPS18 family.Curated
Contains 1 CHCR (clathrin heavy-chain) repeat.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri853 – 94795RING-typeAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG2034. Eukaryota.
ENOG410XRSD. LUCA.
GeneTreeiENSGT00390000015233.
HOVERGENiHBG057876.
InParanoidiQ8R307.
KOiK20181.
OMAiHIYTSMG.
OrthoDBiEOG091G01XI.
PhylomeDBiQ8R307.
TreeFamiTF105704.

Family and domain databases

InterProiIPR000547. Clathrin_H-chain/VPS_repeat.
IPR007810. Pep3_Vps18.
[Graphical view]
PfamiPF00637. Clathrin. 1 hit.
PF05131. Pep3_Vps18. 1 hit.
[Graphical view]
PROSITEiPS50236. CHCR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R307-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASILDEYED SLSRSAVLQT GCPSVGIPHS GYVSAHLEKE VPIFTKQRVD
60 70 80 90 100
FTPSERITSL VVSCNQLCMS LGKDTLLRID LGKASEPNRV ELGRKDDAKV
110 120 130 140 150
HKMFLDHTGS HLLVALSSTE VLYMNRNGQK ARPLARWKGQ LVESVGWNKA
160 170 180 190 200
MGNESSTGPI LVGTAQGQIF EAELSASEGG LFGPAPDLYF RPLYVLNEEG
210 220 230 240 250
GPAPVCSLEA ERGPDGRGFV IATTRQRLFQ FIGRAVEDTE AQGFAGLFAA
260 270 280 290 300
YTDHPPPFRE FPSNLGYSEL AFYTPKLRSA PRAFAWMMGD GVLYGSLDCG
310 320 330 340 350
RPDSLLSEER VWEYPAGVGP GANPPLAIVL TQFHFLLLLA DRVEAVCTLT
360 370 380 390 400
GQVVLRDHFL EKFGPLRHMV KDSSTGHLWA YTERAVFRYH VQREARDVWR
410 420 430 440 450
TYLDMNRFDL AKEYCRERPD CLDTVLAREA DFCFRQHRYL ESARCYALTQ
460 470 480 490 500
SYFEEIALKF LEARQEEALA EFLQRKLAGL KPTERTQATL LTTWLTELYL
510 520 530 540 550
SRLGALQGDP DALTLYRDTR ECFRTFLSSP RHKEWLFASR ASIHELLASH
560 570 580 590 600
GDTEHMVYFA VIMQDYERVV AYHCQHEAYE EALAVLARHR DPQLFYKFSP
610 620 630 640 650
ILIRHIPRQL VDAWIEMGSR LDARQLIPAL VNYSQGGEAQ QVSQAIRYME
660 670 680 690 700
FCVNVLGETE QAIHNYLLSL YARGQPASLL AYLEQAGASP HRVHYDLKYA
710 720 730 740 750
LRLCAEHGHH RACVHVYKVL ELYEEAVDLA LQVDVDLAKQ CADLPEEDEE
760 770 780 790 800
LRKKLWLKIA RHVVQEEEDV QTAMACLASC PLLKIEDVLP FFPDFVTIDH
810 820 830 840 850
FKEAICSSLK AYNHHIQELQ REMEEATASA QRIRRDLQEL RGRYGTVEPQ
860 870 880 890 900
DKCSTCDFPL LNRPFYLFLC GHMFHADCLL QAVRPGLPAY KQARLEELQR
910 920 930 940 950
KLGAAPPPTK GSVKAKEAEA GAAAVGPSRE QLKADLDELV AAECVYCGEL
960 970
MIRSIDRPFI DPQRYEEEHL SWL
Length:973
Mass (Da):110,219
Last modified:June 7, 2004 - v2
Checksum:i6B4E90FC39DC897F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti676 – 6761P → T in BAC28236 (PubMed:16141072).Curated
Sequence conflicti943 – 9431E → G in BAC26302 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029109 mRNA. Translation: BAC26302.1.
AK033333 mRNA. Translation: BAC28236.1.
AK036915 mRNA. Translation: BAC29637.1.
BC026870 mRNA. Translation: AAH26870.1.
BC036129 mRNA. Translation: AAH36129.1.
BC039176 mRNA. Translation: AAH39176.1.
CCDSiCCDS16599.1.
RefSeqiNP_758473.3. NM_172269.3.
UniGeneiMm.294761.

Genome annotation databases

EnsembliENSMUST00000037280; ENSMUSP00000036915; ENSMUSG00000034216.
GeneIDi228545.
KEGGimmu:228545.
UCSCiuc008lto.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029109 mRNA. Translation: BAC26302.1.
AK033333 mRNA. Translation: BAC28236.1.
AK036915 mRNA. Translation: BAC29637.1.
BC026870 mRNA. Translation: AAH26870.1.
BC036129 mRNA. Translation: AAH36129.1.
BC039176 mRNA. Translation: AAH39176.1.
CCDSiCCDS16599.1.
RefSeqiNP_758473.3. NM_172269.3.
UniGeneiMm.294761.

3D structure databases

ProteinModelPortaliQ8R307.
SMRiQ8R307. Positions 284-399.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230740. 17 interactions.
IntActiQ8R307. 3 interactions.
STRINGi10090.ENSMUSP00000036915.

PTM databases

iPTMnetiQ8R307.
PhosphoSiteiQ8R307.

Proteomic databases

EPDiQ8R307.
MaxQBiQ8R307.
PaxDbiQ8R307.
PeptideAtlasiQ8R307.
PRIDEiQ8R307.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037280; ENSMUSP00000036915; ENSMUSG00000034216.
GeneIDi228545.
KEGGimmu:228545.
UCSCiuc008lto.2. mouse.

Organism-specific databases

CTDi57617.
MGIiMGI:2443626. Vps18.

Phylogenomic databases

eggNOGiKOG2034. Eukaryota.
ENOG410XRSD. LUCA.
GeneTreeiENSGT00390000015233.
HOVERGENiHBG057876.
InParanoidiQ8R307.
KOiK20181.
OMAiHIYTSMG.
OrthoDBiEOG091G01XI.
PhylomeDBiQ8R307.
TreeFamiTF105704.

Miscellaneous databases

ChiTaRSiVps18. mouse.
PROiQ8R307.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034216.
CleanExiMM_VPS18.
GenevisibleiQ8R307. MM.

Family and domain databases

InterProiIPR000547. Clathrin_H-chain/VPS_repeat.
IPR007810. Pep3_Vps18.
[Graphical view]
PfamiPF00637. Clathrin. 1 hit.
PF05131. Pep3_Vps18. 1 hit.
[Graphical view]
PROSITEiPS50236. CHCR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVPS18_MOUSE
AccessioniPrimary (citable) accession number: Q8R307
Secondary accession number(s): Q8BGV6, Q8BZX6, Q8C126
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: June 7, 2004
Last modified: September 7, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.