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Protein

Vacuolar protein sorting-associated protein 18 homolog

Gene

Vps18

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (By similarity). Required for fusion of endosomes and autophagosomes with lysosomes (PubMed:14517315, PubMed:22854957). Involved in dendrite development of Pukinje cells (PubMed:22699122).By similarity3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri853 – 947RING-typeAdd BLAST95

GO - Molecular functioni

  • actin binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • protein binding, bridging Source: GO_Central
  • syntaxin binding Source: MGI

GO - Biological processi

Keywordsi

Biological processAutophagy, Protein transport, Transport
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 18 homolog
Gene namesi
Name:Vps18
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2443626 Vps18

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000559072 – 973Vacuolar protein sorting-associated protein 18 homologAdd BLAST972

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei3PhosphoserineBy similarity1
Modified residuei11PhosphoserineBy similarity1
Modified residuei13PhosphoserineBy similarity1
Modified residuei362N6-acetyllysineBy similarity1
Modified residuei689PhosphoserineBy similarity1
Modified residuei912PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8R307
MaxQBiQ8R307
PaxDbiQ8R307
PeptideAtlasiQ8R307
PRIDEiQ8R307

PTM databases

iPTMnetiQ8R307
PhosphoSitePlusiQ8R307

Expressioni

Gene expression databases

BgeeiENSMUSG00000034216
CleanExiMM_VPS18
GenevisibleiQ8R307 MM

Interactioni

Subunit structurei

Core component of at least two putative endosomal tethering complexes, the homotypic fusion and vacuole protein sorting (HOPS) complex and the class C core vacuaole/endosome tethering (CORVET) complex. Their common core is composed of the class C Vps proteins VPS11, VPS16, VPS18 and VPS33A, which in HOPS further associates with VPS39 and VPS41 and in CORVET with VPS8 and TGFBRAP1. Interacts with RAB5C (PubMed:25266290). Interacts with HOOK1 (PubMed:14668490). Interacts with STX7, MON1B (By similarity). Associates with adaptor protein complex 3 (AP-3) and clathrin:AP-3 complexes (PubMed:21411634). Interacts with SYNPO2 (By similarity).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
VIPAS39Q9H9C15EBI-2527788,EBI-749080From Homo sapiens.

GO - Molecular functioni

  • actin binding Source: MGI
  • protein binding, bridging Source: GO_Central
  • syntaxin binding Source: MGI

Protein-protein interaction databases

BioGridi230740, 17 interactors
IntActiQ8R307, 3 interactors
STRINGi10090.ENSMUSP00000036915

Structurei

3D structure databases

ProteinModelPortaliQ8R307
SMRiQ8R307
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati618 – 772CHCRAdd BLAST155

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili454 – 481Sequence analysisAdd BLAST28
Coiled coili802 – 848Sequence analysisAdd BLAST47

Sequence similaritiesi

Belongs to the VPS18 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri853 – 947RING-typeAdd BLAST95

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG2034 Eukaryota
ENOG410XRSD LUCA
GeneTreeiENSGT00390000015233
HOVERGENiHBG057876
InParanoidiQ8R307
KOiK20181
OMAiMFLDPTA
OrthoDBiEOG091G01XI
PhylomeDBiQ8R307
TreeFamiTF105704

Family and domain databases

Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR000547 Clathrin_H-chain/VPS_repeat
IPR007810 Pep3_Vps18
IPR011990 TPR-like_helical_dom_sf
PfamiView protein in Pfam
PF00637 Clathrin, 1 hit
PF05131 Pep3_Vps18, 1 hit
PROSITEiView protein in PROSITE
PS50236 CHCR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R307-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASILDEYED SLSRSAVLQT GCPSVGIPHS GYVSAHLEKE VPIFTKQRVD
60 70 80 90 100
FTPSERITSL VVSCNQLCMS LGKDTLLRID LGKASEPNRV ELGRKDDAKV
110 120 130 140 150
HKMFLDHTGS HLLVALSSTE VLYMNRNGQK ARPLARWKGQ LVESVGWNKA
160 170 180 190 200
MGNESSTGPI LVGTAQGQIF EAELSASEGG LFGPAPDLYF RPLYVLNEEG
210 220 230 240 250
GPAPVCSLEA ERGPDGRGFV IATTRQRLFQ FIGRAVEDTE AQGFAGLFAA
260 270 280 290 300
YTDHPPPFRE FPSNLGYSEL AFYTPKLRSA PRAFAWMMGD GVLYGSLDCG
310 320 330 340 350
RPDSLLSEER VWEYPAGVGP GANPPLAIVL TQFHFLLLLA DRVEAVCTLT
360 370 380 390 400
GQVVLRDHFL EKFGPLRHMV KDSSTGHLWA YTERAVFRYH VQREARDVWR
410 420 430 440 450
TYLDMNRFDL AKEYCRERPD CLDTVLAREA DFCFRQHRYL ESARCYALTQ
460 470 480 490 500
SYFEEIALKF LEARQEEALA EFLQRKLAGL KPTERTQATL LTTWLTELYL
510 520 530 540 550
SRLGALQGDP DALTLYRDTR ECFRTFLSSP RHKEWLFASR ASIHELLASH
560 570 580 590 600
GDTEHMVYFA VIMQDYERVV AYHCQHEAYE EALAVLARHR DPQLFYKFSP
610 620 630 640 650
ILIRHIPRQL VDAWIEMGSR LDARQLIPAL VNYSQGGEAQ QVSQAIRYME
660 670 680 690 700
FCVNVLGETE QAIHNYLLSL YARGQPASLL AYLEQAGASP HRVHYDLKYA
710 720 730 740 750
LRLCAEHGHH RACVHVYKVL ELYEEAVDLA LQVDVDLAKQ CADLPEEDEE
760 770 780 790 800
LRKKLWLKIA RHVVQEEEDV QTAMACLASC PLLKIEDVLP FFPDFVTIDH
810 820 830 840 850
FKEAICSSLK AYNHHIQELQ REMEEATASA QRIRRDLQEL RGRYGTVEPQ
860 870 880 890 900
DKCSTCDFPL LNRPFYLFLC GHMFHADCLL QAVRPGLPAY KQARLEELQR
910 920 930 940 950
KLGAAPPPTK GSVKAKEAEA GAAAVGPSRE QLKADLDELV AAECVYCGEL
960 970
MIRSIDRPFI DPQRYEEEHL SWL
Length:973
Mass (Da):110,219
Last modified:June 7, 2004 - v2
Checksum:i6B4E90FC39DC897F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti676P → T in BAC28236 (PubMed:16141072).Curated1
Sequence conflicti943E → G in BAC26302 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029109 mRNA Translation: BAC26302.1
AK033333 mRNA Translation: BAC28236.1
AK036915 mRNA Translation: BAC29637.1
BC026870 mRNA Translation: AAH26870.1
BC036129 mRNA Translation: AAH36129.1
BC039176 mRNA Translation: AAH39176.1
CCDSiCCDS16599.1
RefSeqiNP_758473.3, NM_172269.3
UniGeneiMm.294761

Genome annotation databases

EnsembliENSMUST00000037280; ENSMUSP00000036915; ENSMUSG00000034216
GeneIDi228545
KEGGimmu:228545
UCSCiuc008lto.2 mouse

Similar proteinsi

Entry informationi

Entry nameiVPS18_MOUSE
AccessioniPrimary (citable) accession number: Q8R307
Secondary accession number(s): Q8BGV6, Q8BZX6, Q8C126
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: June 7, 2004
Last modified: May 23, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families
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