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Protein

Peptidyl-tRNA hydrolase 2, mitochondrial

Gene

Ptrh2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.By similarity

Catalytic activityi

N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidyl-tRNA hydrolase 2, mitochondrial (EC:3.1.1.29)
Short name:
PTH 2
Gene namesi
Name:Ptrh2
Synonyms:Pth2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2444848. Ptrh2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: MGI
  • membrane Source: MGI
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6464MitochondrionSequence analysisAdd
BLAST
Chaini65 – 181117Peptidyl-tRNA hydrolase 2, mitochondrialPRO_0000029863Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki78 – 78Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki83 – 83Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki97 – 97Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki108 – 108Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki117 – 117Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki179 – 179Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Ubiquitinated by PARK2 during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ8R2Y8.
MaxQBiQ8R2Y8.
PaxDbiQ8R2Y8.
PeptideAtlasiQ8R2Y8.
PRIDEiQ8R2Y8.

PTM databases

iPTMnetiQ8R2Y8.
PhosphoSiteiQ8R2Y8.
SwissPalmiQ8R2Y8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000072582.
CleanExiMM_PTH2.
MM_PTRH2.
GenevisibleiQ8R2Y8. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

IntActiQ8R2Y8. 2 interactions.
MINTiMINT-1846414.
STRINGi10090.ENSMUSP00000103656.

Structurei

3D structure databases

ProteinModelPortaliQ8R2Y8.
SMRiQ8R2Y8. Positions 65-181.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PTH2 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3282. Eukaryota.
COG1990. LUCA.
GeneTreeiENSGT00390000015991.
HOGENOMiHOG000227349.
HOVERGENiHBG049458.
InParanoidiQ8R2Y8.
KOiK04794.
OMAiQVAHGAV.
OrthoDBiEOG091G0WC2.
PhylomeDBiQ8R2Y8.
TreeFamiTF324583.

Family and domain databases

Gene3Di3.40.1490.10. 1 hit.
InterProiIPR023476. Pep_tRNA_hydro_II_dom.
IPR002833. PTH2.
[Graphical view]
PfamiPF01981. PTH2. 1 hit.
[Graphical view]
SUPFAMiSSF102462. SSF102462. 1 hit.
TIGRFAMsiTIGR00283. arch_pth2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R2Y8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSKFLTMEY LVHPGTLSLA AGVACGMCLG WGLRSHLGMF PQNSTSEANR
60 70 80 90 100
DTETGTEASI LGESGEYKMI LVVRTDLKMG KGKVAAQCSH AAVSAYKQTQ
110 120 130 140 150
RRSPQVLKEW EYCGQPKVVV KAPDEDTLIQ LLTHAKTLGL TVSLIQDAGR
160 170 180
TQIEPGSRTV LGIGPGPVEL IDEVTGHLKL Y
Length:181
Mass (Da):19,527
Last modified:June 1, 2002 - v1
Checksum:iC341176DE560C2B8
GO

Sequence cautioni

The sequence BAC26006 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti171 – 1711I → T in BAC30159 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028555 mRNA. Translation: BAC26006.1. Different initiation.
AK031620 mRNA. Translation: BAC27482.1.
AK038888 mRNA. Translation: BAC30159.1.
BC026947 mRNA. Translation: AAH26947.1.
CCDSiCCDS48876.1.
RefSeqiNP_001092280.1. NM_001098810.2.
NP_778169.1. NM_175004.2.
UniGeneiMm.24868.

Genome annotation databases

EnsembliENSMUST00000108021; ENSMUSP00000103656; ENSMUSG00000072582.
ENSMUST00000108022; ENSMUSP00000103657; ENSMUSG00000072582.
GeneIDi217057.
KEGGimmu:217057.
UCSCiuc007ksy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028555 mRNA. Translation: BAC26006.1. Different initiation.
AK031620 mRNA. Translation: BAC27482.1.
AK038888 mRNA. Translation: BAC30159.1.
BC026947 mRNA. Translation: AAH26947.1.
CCDSiCCDS48876.1.
RefSeqiNP_001092280.1. NM_001098810.2.
NP_778169.1. NM_175004.2.
UniGeneiMm.24868.

3D structure databases

ProteinModelPortaliQ8R2Y8.
SMRiQ8R2Y8. Positions 65-181.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8R2Y8. 2 interactions.
MINTiMINT-1846414.
STRINGi10090.ENSMUSP00000103656.

PTM databases

iPTMnetiQ8R2Y8.
PhosphoSiteiQ8R2Y8.
SwissPalmiQ8R2Y8.

Proteomic databases

EPDiQ8R2Y8.
MaxQBiQ8R2Y8.
PaxDbiQ8R2Y8.
PeptideAtlasiQ8R2Y8.
PRIDEiQ8R2Y8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000108021; ENSMUSP00000103656; ENSMUSG00000072582.
ENSMUST00000108022; ENSMUSP00000103657; ENSMUSG00000072582.
GeneIDi217057.
KEGGimmu:217057.
UCSCiuc007ksy.1. mouse.

Organism-specific databases

CTDi51651.
MGIiMGI:2444848. Ptrh2.

Phylogenomic databases

eggNOGiKOG3282. Eukaryota.
COG1990. LUCA.
GeneTreeiENSGT00390000015991.
HOGENOMiHOG000227349.
HOVERGENiHBG049458.
InParanoidiQ8R2Y8.
KOiK04794.
OMAiQVAHGAV.
OrthoDBiEOG091G0WC2.
PhylomeDBiQ8R2Y8.
TreeFamiTF324583.

Miscellaneous databases

PROiQ8R2Y8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000072582.
CleanExiMM_PTH2.
MM_PTRH2.
GenevisibleiQ8R2Y8. MM.

Family and domain databases

Gene3Di3.40.1490.10. 1 hit.
InterProiIPR023476. Pep_tRNA_hydro_II_dom.
IPR002833. PTH2.
[Graphical view]
PfamiPF01981. PTH2. 1 hit.
[Graphical view]
SUPFAMiSSF102462. SSF102462. 1 hit.
TIGRFAMsiTIGR00283. arch_pth2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPTH2_MOUSE
AccessioniPrimary (citable) accession number: Q8R2Y8
Secondary accession number(s): Q8BI01, Q8BI31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: June 1, 2002
Last modified: September 7, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.