Reviewed, UniProtKB/Swiss-Prot Q8R2Y8 (PTH2_MOUSE)

Names and origin

Protein names

Recommended name:
    Peptidyl-tRNA hydrolase 2, mitochondrial
      Short name=PTH 2
    EC=3.1.1.29

Gene names

Name:	Ptrh2
Synonyms:	Pth2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis By similarity.
Catalytic activity	N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA.
Subunit structure	Monomer By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the PTH2 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from direct assay. Source: MGI
Molecular function	aminoacyl-tRNA hydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 64	64	Mitochondrion Potential
	Chain	65 – 181	117	Peptidyl-tRNA hydrolase 2, mitochondrial		PRO_0000029863
Amino acid modifications
	Modified residue	59	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	171	1	I → T in BAC30159. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8R2Y8-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: C341176DE560C2B8	FASTA	181	19,527
	10 20 30 40 50 60 MLSKFLTMEY LVHPGTLSLA AGVACGMCLG WGLRSHLGMF PQNSTSEANR DTETGTEASI 70 80 90 100 110 120 LGESGEYKMI LVVRTDLKMG KGKVAAQCSH AAVSAYKQTQ RRSPQVLKEW EYCGQPKVVV 130 140 150 160 170 180 KAPDEDTLIQ LLTHAKTLGL TVSLIQDAGR TQIEPGSRTV LGIGPGPVEL IDEVTGHLKL Y

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Hypothalamus, Skin and Testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK028555 mRNA. Translation: BAC26006.1. Different initiation. AK031620 mRNA. Translation: BAC27482.1. AK038888 mRNA. Translation: BAC30159.1. BC026947 mRNA. Translation: AAH26947.1.
IPI	IPI00853967.
RefSeq	NP_001092280.1. NP_778169.1.
UniGene	Mm.24868
3D structure databases
SMR	Q8R2Y8. Positions 65-181.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8R2Y8.
PTM databases
PhosphoSite	Q8R2Y8.
Proteomic databases
PRIDE	Q8R2Y8.
Genome annotation databases
Ensembl	ENSMUST00000100665; ENSMUSP00000098230; ENSMUSG00000072582; Mus musculus. [Genome view] ENSMUST00000108021; ENSMUSP00000103656; ENSMUSG00000072582; Mus musculus. [Genome view] ENSMUST00000108022; ENSMUSP00000103657; ENSMUSG00000072582; Mus musculus. [Genome view]
GeneID	217057.
KEGG	mmu:217057.
UCSC	uc007ksz.1. mouse.
Organism-specific databases
CTD	217057.
MGI	MGI:2444848. Ptrh2.
Phylogenomic databases
HOGENOM	Q8R2Y8.
HOVERGEN	Q8R2Y8.
OMA	MEYLVHP.
Enzyme and pathway databases
BRENDA	3.1.1.29. 244.
Gene expression databases
ArrayExpress	Q8R2Y8.
Bgee	Q8R2Y8.
CleanEx	MM_PTH2. MM_PTRH2.
Genevestigator	Q8R2Y8.
GermOnline	ENSMUSG00000072582. Mus musculus.
Family and domain databases
InterPro	IPR002833. Pep_tRNA_hydro_PTH2. [Graphical view]
Pfam	PF01981. PTH2. 1 hit. [Graphical view]
ProDom	PD010667. UPF0099. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00283. Pep_tRNA_hydro_PTH2. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	375534.
SOURCE	Search...

Entry information

Entry name

PTH2_MOUSE

Accession

Primary (citable) accession number: Q8R2Y8
Secondary accession number(s): Q8BI01, Q8BI31

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)