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Protein

Cell surface glycoprotein MUC18

Gene

Mcam

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration (By similarity).By similarity

GO - Biological processi

Keywordsi

Biological processCell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Cell surface glycoprotein MUC18
Alternative name(s):
Gicerin
Melanoma cell adhesion molecule
Melanoma-associated antigen MUC18
CD_antigen: CD146
Gene namesi
Name:Mcam
Synonyms:Muc18
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1933966. Mcam.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 563ExtracellularSequence analysisAdd BLAST540
Transmembranei564 – 584HelicalSequence analysisAdd BLAST21
Topological domaini585 – 648CytoplasmicSequence analysisAdd BLAST64

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23By similarityAdd BLAST23
ChainiPRO_000004546024 – 648Cell surface glycoprotein MUC18Add BLAST625

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi50 ↔ 118PROSITE-ProRule annotation
Glycosylationi58N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi163 ↔ 225PROSITE-ProRule annotation
Disulfide bondi274 ↔ 322PROSITE-ProRule annotation
Disulfide bondi367 ↔ 409PROSITE-ProRule annotation
Disulfide bondi454 ↔ 501PROSITE-ProRule annotation
Glycosylationi510N-linked (GlcNAc...) asparagine1 Publication1
Modified residuei608PhosphoserineCombined sources1
Modified residuei616PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8R2Y2.
PeptideAtlasiQ8R2Y2.
PRIDEiQ8R2Y2.

PTM databases

iPTMnetiQ8R2Y2.
PhosphoSitePlusiQ8R2Y2.
SwissPalmiQ8R2Y2.

Expressioni

Tissue specificityi

Detected in melanoma cell lines.1 Publication

Gene expression databases

BgeeiENSMUSG00000032135.
CleanExiMM_MCAM.
ExpressionAtlasiQ8R2Y2. baseline and differential.
GenevisibleiQ8R2Y2. MM.

Interactioni

Protein-protein interaction databases

IntActiQ8R2Y2. 1 interactor.
MINTiMINT-4128850.
STRINGi10090.ENSMUSP00000034650.

Structurei

3D structure databases

ProteinModelPortaliQ8R2Y2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 131Ig-like V-type 1Add BLAST108
Domaini141 – 244Ig-like V-type 2Add BLAST104
Domaini246 – 332Ig-like C2-type 1Add BLAST87
Domaini337 – 426Ig-like C2-type 2Add BLAST90
Domaini432 – 512Ig-like C2-type 3Add BLAST81

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEC2. Eukaryota.
ENOG4110RPG. LUCA.
GeneTreeiENSGT00530000063457.
HOGENOMiHOG000015427.
HOVERGENiHBG002808.
InParanoidiQ8R2Y2.
KOiK06534.
OMAiGDQGEKY.
OrthoDBiEOG091G072E.
PhylomeDBiQ8R2Y2.
TreeFamiTF330534.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiView protein in InterPro
IPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR036179. Ig-like_dom_sf.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
PfamiView protein in Pfam
PF08205. C2-set_2. 1 hit.
PF07679. I-set. 1 hit.
PF07686. V-set. 1 hit.
SMARTiView protein in SMART
SM00409. IG. 4 hits.
SM00408. IGc2. 4 hits.
SUPFAMiSSF48726. SSF48726. 5 hits.
PROSITEiView protein in PROSITE
PS50835. IG_LIKE. 4 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R2Y2-1) [UniParc]FASTAAdd to basket
Also known as: L-gicerin

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGLPKLVCVF LFAACCCCRR AAGVPGEEKQ PVPTPDLVEA EVGSTALLKC
60 70 80 90 100
GPSRASGNFS QVDWFLIHKE RQILIFRVHQ GKGQREPGEY EHRLSLQDSV
110 120 130 140 150
ATLALSHVTP HDERMFLCKS KRPRLQDHYV ELQVFKAPEE PTIQANVVGI
160 170 180 190 200
HVDRQELREV ATCVGRNGYP IPQVLWYKNS LPLQEEENRV HIQSSQIVES
210 220 230 240 250
SGLYTLKSVL SARLVKEDKD AQFYCELSYR LPSGNHMKES KEVTVPVFYP
260 270 280 290 300
AEKVWVEVEP VGLLKEGDHV TIRCLTDGNP QPHFTINKKD PSTGEMEEES
310 320 330 340 350
TDENGLLSLE PAEKHHSGLY QCQSLDLETT ITLSSDPLEL LVNYVSDVQV
360 370 380 390 400
NPTAPEVQEG ESLTLTCEAE SNQDLEFEWL RDKTGQLLGK GPVLQLNNVR
410 420 430 440 450
REAGGRYLCM ASVPRVPGLN RTQLVSVGIF GSPWMALKER KVWVQENAVL
460 470 480 490 500
NLSCEASGHP QPTISWNVNG SATEWNPDPQ TVVSTLNVLV TPELLETGAE
510 520 530 540 550
CTASNSLGSN TTTIVLKLVT LTTLIPDSSQ TTGLSTLTVS PHTRANSTST
560 570 580 590 600
EKKLPQPESK GVVIVAVIVC TLVLAVLGAA LYFFYKKGKL PCGRSGKQEI
610 620 630 640
TLPPTRKSEF VVEVKSDKLP EEMALLQGSN GDKRAPGDQG EKYIDLRH
Length:648
Mass (Da):71,545
Last modified:June 1, 2002 - v1
Checksum:iE468DABDB56C28FD
GO
Isoform 2 (identifier: Q8R2Y2-2) [UniParc]FASTAAdd to basket
Also known as: S-gicerin

The sequence of this isoform differs from the canonical sequence as follows:
     600-648: ITLPPTRKSEFVVEVKSDKLPEEMALLQGSNGDKRAPGDQGEKYIDLRH → MERNTSI

Show »
Length:606
Mass (Da):66,901
Checksum:iD53CBAC0A5F2967D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti584F → L in BAB16050 (Ref. 1) Curated1
Sequence conflicti584F → L in BAB16051 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016940600 – 648ITLPP…IDLRH → MERNTSI in isoform 2. 2 PublicationsAdd BLAST49

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035508 mRNA. Translation: BAB16050.1.
AB035509 mRNA. Translation: BAB16051.1.
BC026985 mRNA. Translation: AAH26985.1.
AJ297450 mRNA. Translation: CAB97362.1.
AJ297451 mRNA. Translation: CAB97363.1.
CCDSiCCDS23097.1. [Q8R2Y2-1]
RefSeqiNP_075548.2. NM_023061.2. [Q8R2Y2-1]
XP_006510779.1. XM_006510716.2. [Q8R2Y2-2]
UniGeneiMm.275003.

Genome annotation databases

EnsembliENSMUST00000034650; ENSMUSP00000034650; ENSMUSG00000032135. [Q8R2Y2-1]
ENSMUST00000098852; ENSMUSP00000096451; ENSMUSG00000032135. [Q8R2Y2-2]
GeneIDi84004.
KEGGimmu:84004.
UCSCiuc009pbw.2. mouse. [Q8R2Y2-1]
uc009pbx.2. mouse. [Q8R2Y2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMUC18_MOUSE
AccessioniPrimary (citable) accession number: Q8R2Y2
Secondary accession number(s): Q9EPF1
, Q9ESS7, Q9JHQ2, Q9JHQ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: June 1, 2002
Last modified: October 25, 2017
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot