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Q8R2X8

- GO45_MOUSE

UniProt

Q8R2X8 - GO45_MOUSE

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Protein

Golgin-45

Gene

Blzf1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.By similarity

GO - Biological processi

  1. Golgi organization Source: InterPro
  2. Golgi to plasma membrane protein transport Source: InterPro
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_257344. Golgi Cisternae Pericentriolar Stack Reorganization.

Names & Taxonomyi

Protein namesi
Recommended name:
Golgin-45
Alternative name(s):
Basic leucine zipper nuclear factor 1
Gene namesi
Name:Blzf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1201607. Blzf1.

Subcellular locationi

Golgi apparatus lumen By similarity. Nucleus By similarity. Cytoplasm By similarity
Note: Also found in the nucleus and in the cytoplasm.By similarity

GO - Cellular componenti

  1. Golgi apparatus Source: MGI
  2. Golgi membrane Source: Ensembl
  3. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 403403Golgin-45PRO_0000087540Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei53 – 531PhosphoserineBy similarity
Modified residuei356 – 3561PhosphoserineBy similarity

Post-translational modificationi

ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination (By similarity).By similarity
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation.By similarity

Keywords - PTMi

ADP-ribosylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ8R2X8.
PaxDbiQ8R2X8.
PRIDEiQ8R2X8.

PTM databases

PhosphoSiteiQ8R2X8.

Expressioni

Gene expression databases

BgeeiQ8R2X8.
CleanExiMM_BLZF1.
GenevestigatoriQ8R2X8.

Interactioni

Subunit structurei

Interacts with GORASP2 and with the GTP-bound form of RAB2, but not with other Golgi Rab proteins. GORASP2 and BLZF1 form a RAB2 effector complex on medial Golgi (By similarity).By similarity

Protein-protein interaction databases

BioGridi211404. 3 interactions.
IntActiQ8R2X8. 5 interactions.
MINTiMINT-4096412.

Structurei

3D structure databases

ProteinModelPortaliQ8R2X8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni397 – 4037Essential for the interaction with GORASP2By similarity

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili123 – 21694Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi22 – 265Tankyrase-binding motifBy similarity

Domaini

The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.By similarity

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG279660.
GeneTreeiENSGT00390000009400.
HOGENOMiHOG000088649.
HOVERGENiHBG051750.
InParanoidiQ8R2X8.
OMAiQRQNRDA.
OrthoDBiEOG7P5T13.
PhylomeDBiQ8R2X8.
TreeFamiTF317238.

Family and domain databases

InterProiIPR027095. Golgin-45.
IPR013183. Hsk3_like.
[Graphical view]
PANTHERiPTHR13066. PTHR13066. 1 hit.
PfamiPF08227. DASH_Hsk3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8R2X8-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEKMTTLKSS ENKGILTSTP IRGAGDGMET EEPPKSVEVT HGVQPINQHV
60 70 80 90 100
LPSPRKKVSS DSPGVLQLGK ILNERTVEVE AVRIFVPKAA ITHDIPTKNT
110 120 130 140 150
KVKSLGHHRE ELHNQAEVVT DPRKELSEVK KVLEKLKNSE RRLLQDKEGL
160 170 180 190 200
SNQLRVQTEI NRELKKLLVA SVGDDPQYHF ERLAREKNQL ILENEALGRN
210 220 230 240 250
TAQLSEQLER MSIQCDVWRS KFLASRVMAD ELTNFRVVLQ RQNRDAQSAI
260 270 280 290 300
QDLLSEREQF RQEMTSTQKF LEELLVSLQW GREQTYSPNT QPHSTADLAL
310 320 330 340 350
TNHGLAQAIH AHLLGNVGIS HQKKIPTTVE FCSTPAEKMA EKVLRILDPV
360 370 380 390 400
ACTESSPDNQ FAESSPTTLL TTKKNIGRFH PYTRYENITF NCCNHCQGEL

IAL
Length:403
Mass (Da):45,541
Last modified:August 16, 2004 - v2
Checksum:iF87A9752AEEC3559
GO
Isoform 2 (identifier: Q8R2X8-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     14-14: G → GKQEMMKG

Show »
Length:410
Mass (Da):46,374
Checksum:i59231CC8897468FB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti320 – 3201S → R in AAH27025. (PubMed:15489334)Curated
Sequence conflicti346 – 3461I → N in AAH27025. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei14 – 141G → GKQEMMKG in isoform 2. 1 PublicationVSP_011188

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005489 mRNA. Translation: BAB24074.1.
AK029787 mRNA. Translation: BAC26617.1.
AK167493 mRNA. Translation: BAE39571.1.
BC027025 mRNA. Translation: AAH27025.1.
CCDSiCCDS15435.1. [Q8R2X8-2]
CCDS48423.1. [Q8R2X8-1]
RefSeqiNP_001153680.1. NM_001160208.1. [Q8R2X8-1]
NP_001153681.1. NM_001160209.1. [Q8R2X8-1]
NP_079781.2. NM_025505.4. [Q8R2X8-2]
UniGeneiMm.33285.

Genome annotation databases

EnsembliENSMUST00000027866; ENSMUSP00000027866; ENSMUSG00000026577. [Q8R2X8-2]
ENSMUST00000086032; ENSMUSP00000083196; ENSMUSG00000026577. [Q8R2X8-1]
ENSMUST00000120447; ENSMUSP00000113479; ENSMUSG00000026577. [Q8R2X8-1]
GeneIDi66352.
KEGGimmu:66352.
UCSCiuc007dig.1. mouse. [Q8R2X8-2]
uc007dih.1. mouse. [Q8R2X8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005489 mRNA. Translation: BAB24074.1 .
AK029787 mRNA. Translation: BAC26617.1 .
AK167493 mRNA. Translation: BAE39571.1 .
BC027025 mRNA. Translation: AAH27025.1 .
CCDSi CCDS15435.1. [Q8R2X8-2 ]
CCDS48423.1. [Q8R2X8-1 ]
RefSeqi NP_001153680.1. NM_001160208.1. [Q8R2X8-1 ]
NP_001153681.1. NM_001160209.1. [Q8R2X8-1 ]
NP_079781.2. NM_025505.4. [Q8R2X8-2 ]
UniGenei Mm.33285.

3D structure databases

ProteinModelPortali Q8R2X8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 211404. 3 interactions.
IntActi Q8R2X8. 5 interactions.
MINTi MINT-4096412.

PTM databases

PhosphoSitei Q8R2X8.

Proteomic databases

MaxQBi Q8R2X8.
PaxDbi Q8R2X8.
PRIDEi Q8R2X8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027866 ; ENSMUSP00000027866 ; ENSMUSG00000026577 . [Q8R2X8-2 ]
ENSMUST00000086032 ; ENSMUSP00000083196 ; ENSMUSG00000026577 . [Q8R2X8-1 ]
ENSMUST00000120447 ; ENSMUSP00000113479 ; ENSMUSG00000026577 . [Q8R2X8-1 ]
GeneIDi 66352.
KEGGi mmu:66352.
UCSCi uc007dig.1. mouse. [Q8R2X8-2 ]
uc007dih.1. mouse. [Q8R2X8-1 ]

Organism-specific databases

CTDi 8548.
MGIi MGI:1201607. Blzf1.

Phylogenomic databases

eggNOGi NOG279660.
GeneTreei ENSGT00390000009400.
HOGENOMi HOG000088649.
HOVERGENi HBG051750.
InParanoidi Q8R2X8.
OMAi QRQNRDA.
OrthoDBi EOG7P5T13.
PhylomeDBi Q8R2X8.
TreeFami TF317238.

Enzyme and pathway databases

Reactomei REACT_257344. Golgi Cisternae Pericentriolar Stack Reorganization.

Miscellaneous databases

NextBioi 321405.
PROi Q8R2X8.
SOURCEi Search...

Gene expression databases

Bgeei Q8R2X8.
CleanExi MM_BLZF1.
Genevestigatori Q8R2X8.

Family and domain databases

InterProi IPR027095. Golgin-45.
IPR013183. Hsk3_like.
[Graphical view ]
PANTHERi PTHR13066. PTHR13066. 1 hit.
Pfami PF08227. DASH_Hsk3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Placenta and Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N-3.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiGO45_MOUSE
AccessioniPrimary (citable) accession number: Q8R2X8
Secondary accession number(s): Q3TJC4, Q8C0U6, Q9DAV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: November 26, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was also thought to be a potential transcription factor, localized in the nucleus.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3