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Q8R2X8

- GO45_MOUSE

UniProt

Q8R2X8 - GO45_MOUSE

Protein

Golgin-45

Gene

Blzf1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 2 (16 Aug 2004)
      Previous versions | rss
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    Functioni

    Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.By similarity

    GO - Molecular functioni

    1. protein binding Source: MGI

    GO - Biological processi

    1. Golgi organization Source: InterPro
    2. Golgi to plasma membrane protein transport Source: InterPro

    Keywords - Biological processi

    ER-Golgi transport, Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Golgin-45
    Alternative name(s):
    Basic leucine zipper nuclear factor 1
    Gene namesi
    Name:Blzf1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1201607. Blzf1.

    Subcellular locationi

    Golgi apparatus lumen By similarity. Nucleus By similarity. Cytoplasm By similarity
    Note: Also found in the nucleus and in the cytoplasm.By similarity

    GO - Cellular componenti

    1. Golgi apparatus Source: MGI
    2. Golgi lumen Source: UniProtKB-SubCell
    3. Golgi membrane Source: Ensembl
    4. nucleus Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Golgi apparatus, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 403403Golgin-45PRO_0000087540Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei53 – 531PhosphoserineBy similarity
    Modified residuei356 – 3561PhosphoserineBy similarity

    Post-translational modificationi

    ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination By similarity.By similarity
    Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation.By similarity

    Keywords - PTMi

    ADP-ribosylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiQ8R2X8.
    PRIDEiQ8R2X8.

    PTM databases

    PhosphoSiteiQ8R2X8.

    Expressioni

    Gene expression databases

    BgeeiQ8R2X8.
    CleanExiMM_BLZF1.
    GenevestigatoriQ8R2X8.

    Interactioni

    Subunit structurei

    Interacts with GORASP2 and with the GTP-bound form of RAB2, but not with other Golgi Rab proteins. GORASP2 and BLZF1 form a RAB2 effector complex on medial Golgi By similarity.By similarity

    Protein-protein interaction databases

    BioGridi211404. 3 interactions.
    IntActiQ8R2X8. 5 interactions.
    MINTiMINT-4096412.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8R2X8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni397 – 4037Essential for the interaction with GORASP2By similarity

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili123 – 21694Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi22 – 265Tankyrase-binding motifBy similarity

    Domaini

    The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.By similarity

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG279660.
    GeneTreeiENSGT00390000009400.
    HOGENOMiHOG000088649.
    HOVERGENiHBG051750.
    OMAiQRQNRDA.
    OrthoDBiEOG7P5T13.
    PhylomeDBiQ8R2X8.
    TreeFamiTF317238.

    Family and domain databases

    InterProiIPR027095. Golgin-45.
    IPR013183. Hsk3_like.
    [Graphical view]
    PANTHERiPTHR13066. PTHR13066. 1 hit.
    PfamiPF08227. DASH_Hsk3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8R2X8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEKMTTLKSS ENKGILTSTP IRGAGDGMET EEPPKSVEVT HGVQPINQHV    50
    LPSPRKKVSS DSPGVLQLGK ILNERTVEVE AVRIFVPKAA ITHDIPTKNT 100
    KVKSLGHHRE ELHNQAEVVT DPRKELSEVK KVLEKLKNSE RRLLQDKEGL 150
    SNQLRVQTEI NRELKKLLVA SVGDDPQYHF ERLAREKNQL ILENEALGRN 200
    TAQLSEQLER MSIQCDVWRS KFLASRVMAD ELTNFRVVLQ RQNRDAQSAI 250
    QDLLSEREQF RQEMTSTQKF LEELLVSLQW GREQTYSPNT QPHSTADLAL 300
    TNHGLAQAIH AHLLGNVGIS HQKKIPTTVE FCSTPAEKMA EKVLRILDPV 350
    ACTESSPDNQ FAESSPTTLL TTKKNIGRFH PYTRYENITF NCCNHCQGEL 400
    IAL 403
    Length:403
    Mass (Da):45,541
    Last modified:August 16, 2004 - v2
    Checksum:iF87A9752AEEC3559
    GO
    Isoform 2 (identifier: Q8R2X8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         14-14: G → GKQEMMKG

    Show »
    Length:410
    Mass (Da):46,374
    Checksum:i59231CC8897468FB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti320 – 3201S → R in AAH27025. (PubMed:15489334)Curated
    Sequence conflicti346 – 3461I → N in AAH27025. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei14 – 141G → GKQEMMKG in isoform 2. 1 PublicationVSP_011188

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK005489 mRNA. Translation: BAB24074.1.
    AK029787 mRNA. Translation: BAC26617.1.
    AK167493 mRNA. Translation: BAE39571.1.
    BC027025 mRNA. Translation: AAH27025.1.
    CCDSiCCDS15435.1. [Q8R2X8-2]
    CCDS48423.1. [Q8R2X8-1]
    RefSeqiNP_001153680.1. NM_001160208.1. [Q8R2X8-1]
    NP_001153681.1. NM_001160209.1. [Q8R2X8-1]
    NP_079781.2. NM_025505.4. [Q8R2X8-2]
    UniGeneiMm.33285.

    Genome annotation databases

    EnsembliENSMUST00000027866; ENSMUSP00000027866; ENSMUSG00000026577. [Q8R2X8-2]
    ENSMUST00000086032; ENSMUSP00000083196; ENSMUSG00000026577. [Q8R2X8-1]
    ENSMUST00000120447; ENSMUSP00000113479; ENSMUSG00000026577. [Q8R2X8-1]
    GeneIDi66352.
    KEGGimmu:66352.
    UCSCiuc007dig.1. mouse. [Q8R2X8-2]
    uc007dih.1. mouse. [Q8R2X8-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK005489 mRNA. Translation: BAB24074.1 .
    AK029787 mRNA. Translation: BAC26617.1 .
    AK167493 mRNA. Translation: BAE39571.1 .
    BC027025 mRNA. Translation: AAH27025.1 .
    CCDSi CCDS15435.1. [Q8R2X8-2 ]
    CCDS48423.1. [Q8R2X8-1 ]
    RefSeqi NP_001153680.1. NM_001160208.1. [Q8R2X8-1 ]
    NP_001153681.1. NM_001160209.1. [Q8R2X8-1 ]
    NP_079781.2. NM_025505.4. [Q8R2X8-2 ]
    UniGenei Mm.33285.

    3D structure databases

    ProteinModelPortali Q8R2X8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 211404. 3 interactions.
    IntActi Q8R2X8. 5 interactions.
    MINTi MINT-4096412.

    PTM databases

    PhosphoSitei Q8R2X8.

    Proteomic databases

    PaxDbi Q8R2X8.
    PRIDEi Q8R2X8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027866 ; ENSMUSP00000027866 ; ENSMUSG00000026577 . [Q8R2X8-2 ]
    ENSMUST00000086032 ; ENSMUSP00000083196 ; ENSMUSG00000026577 . [Q8R2X8-1 ]
    ENSMUST00000120447 ; ENSMUSP00000113479 ; ENSMUSG00000026577 . [Q8R2X8-1 ]
    GeneIDi 66352.
    KEGGi mmu:66352.
    UCSCi uc007dig.1. mouse. [Q8R2X8-2 ]
    uc007dih.1. mouse. [Q8R2X8-1 ]

    Organism-specific databases

    CTDi 8548.
    MGIi MGI:1201607. Blzf1.

    Phylogenomic databases

    eggNOGi NOG279660.
    GeneTreei ENSGT00390000009400.
    HOGENOMi HOG000088649.
    HOVERGENi HBG051750.
    OMAi QRQNRDA.
    OrthoDBi EOG7P5T13.
    PhylomeDBi Q8R2X8.
    TreeFami TF317238.

    Miscellaneous databases

    NextBioi 321405.
    PROi Q8R2X8.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8R2X8.
    CleanExi MM_BLZF1.
    Genevestigatori Q8R2X8.

    Family and domain databases

    InterProi IPR027095. Golgin-45.
    IPR013183. Hsk3_like.
    [Graphical view ]
    PANTHERi PTHR13066. PTHR13066. 1 hit.
    Pfami PF08227. DASH_Hsk3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Placenta and Testis.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N-3.
      Tissue: Mammary tumor.

    Entry informationi

    Entry nameiGO45_MOUSE
    AccessioniPrimary (citable) accession number: Q8R2X8
    Secondary accession number(s): Q3TJC4, Q8C0U6, Q9DAV7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 88 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    Was also thought to be a potential transcription factor, localized in the nucleus.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3