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Q8R2X8 (GO45_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Golgin-45
Alternative name(s):
Basic leucine zipper nuclear factor 1
Gene names
Name:Blzf1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length403 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface By similarity.

Subunit structure

Interacts with GORASP2 and with the GTP-bound form of RAB2, but not with other Golgi Rab proteins. GORASP2 and BLZF1 form a RAB2 effector complex on medial Golgi By similarity.

Subcellular location

Golgi apparatus lumen By similarity. Nucleus By similarity. Cytoplasm By similarity. Note: Also found in the nucleus and in the cytoplasm By similarity.

Domain

The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2 By similarity.

Post-translational modification

ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination By similarity.

Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation By similarity.

Caution

Was also thought to be a potential transcription factor, localized in the nucleus.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8R2X8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8R2X8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     14-14: G → GKQEMMKG

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 403403Golgin-45
PRO_0000087540

Regions

Region397 – 4037Essential for the interaction with GORASP2 By similarity
Coiled coil123 – 21694 Potential
Motif22 – 265Tankyrase-binding motif By similarity

Amino acid modifications

Modified residue531Phosphoserine By similarity
Modified residue3561Phosphoserine By similarity

Natural variations

Alternative sequence141G → GKQEMMKG in isoform 2.
VSP_011188

Experimental info

Sequence conflict3201S → R in AAH27025. Ref.2
Sequence conflict3461I → N in AAH27025. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified August 16, 2004. Version 2.
Checksum: F87A9752AEEC3559

FASTA40345,541
        10         20         30         40         50         60 
MEKMTTLKSS ENKGILTSTP IRGAGDGMET EEPPKSVEVT HGVQPINQHV LPSPRKKVSS 

        70         80         90        100        110        120 
DSPGVLQLGK ILNERTVEVE AVRIFVPKAA ITHDIPTKNT KVKSLGHHRE ELHNQAEVVT 

       130        140        150        160        170        180 
DPRKELSEVK KVLEKLKNSE RRLLQDKEGL SNQLRVQTEI NRELKKLLVA SVGDDPQYHF 

       190        200        210        220        230        240 
ERLAREKNQL ILENEALGRN TAQLSEQLER MSIQCDVWRS KFLASRVMAD ELTNFRVVLQ 

       250        260        270        280        290        300 
RQNRDAQSAI QDLLSEREQF RQEMTSTQKF LEELLVSLQW GREQTYSPNT QPHSTADLAL 

       310        320        330        340        350        360 
TNHGLAQAIH AHLLGNVGIS HQKKIPTTVE FCSTPAEKMA EKVLRILDPV ACTESSPDNQ 

       370        380        390        400 
FAESSPTTLL TTKKNIGRFH PYTRYENITF NCCNHCQGEL IAL 

« Hide

Isoform 2 [UniParc].

Checksum: 59231CC8897468FB
Show »

FASTA41046,374

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Placenta and Testis.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N-3.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK005489 mRNA. Translation: BAB24074.1.
AK029787 mRNA. Translation: BAC26617.1.
AK167493 mRNA. Translation: BAE39571.1.
BC027025 mRNA. Translation: AAH27025.1.
CCDSCCDS15435.1. [Q8R2X8-2]
CCDS48423.1. [Q8R2X8-1]
RefSeqNP_001153680.1. NM_001160208.1. [Q8R2X8-1]
NP_001153681.1. NM_001160209.1. [Q8R2X8-1]
NP_079781.2. NM_025505.4. [Q8R2X8-2]
UniGeneMm.33285.

3D structure databases

ProteinModelPortalQ8R2X8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid211404. 3 interactions.
IntActQ8R2X8. 5 interactions.
MINTMINT-4096412.

PTM databases

PhosphoSiteQ8R2X8.

Proteomic databases

PaxDbQ8R2X8.
PRIDEQ8R2X8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000027866; ENSMUSP00000027866; ENSMUSG00000026577. [Q8R2X8-2]
ENSMUST00000086032; ENSMUSP00000083196; ENSMUSG00000026577. [Q8R2X8-1]
ENSMUST00000120447; ENSMUSP00000113479; ENSMUSG00000026577. [Q8R2X8-1]
GeneID66352.
KEGGmmu:66352.
UCSCuc007dig.1. mouse. [Q8R2X8-2]
uc007dih.1. mouse. [Q8R2X8-1]

Organism-specific databases

CTD8548.
MGIMGI:1201607. Blzf1.

Phylogenomic databases

eggNOGNOG279660.
GeneTreeENSGT00390000009400.
HOGENOMHOG000088649.
HOVERGENHBG051750.
OMAQRQNRDA.
OrthoDBEOG7P5T13.
PhylomeDBQ8R2X8.
TreeFamTF317238.

Gene expression databases

BgeeQ8R2X8.
CleanExMM_BLZF1.
GenevestigatorQ8R2X8.

Family and domain databases

InterProIPR027095. Golgin-45.
IPR013183. Hsk3_like.
[Graphical view]
PANTHERPTHR13066. PTHR13066. 1 hit.
PfamPF08227. DASH_Hsk3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio321405.
PROQ8R2X8.
SOURCESearch...

Entry information

Entry nameGO45_MOUSE
AccessionPrimary (citable) accession number: Q8R2X8
Secondary accession number(s): Q3TJC4, Q8C0U6, Q9DAV7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: July 9, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot