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Protein

N-terminal Xaa-Pro-Lys N-methyltransferase 1

Gene

Ntmt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Gly/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of the exposed alpha-amino group of the Ala, Gly or Ser residue in the [Ala/Gly/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif (PubMed:20668449). Some of the substrates may be primed by METTL11B-mediated monomethylation. Catalyzes the trimethylation of the N-terminal Gly in CENPA (after removal of Met-1) (By similarity). Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1 (PubMed:20668449).By similarity1 Publication

Catalytic activityi

3 S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] = 3 S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein].1 Publication
2 S-adenosyl-L-methionine + N-terminal-PPK-[protein] = 2 S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein].1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei74S-adenosyl-L-methionineBy similarity1
Binding sitei135S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
N-terminal Xaa-Pro-Lys N-methyltransferase 1 (EC:2.1.1.2441 Publication)
Alternative name(s):
Alpha N-terminal protein methyltransferase 1A
Methyltransferase-like protein 11A
X-Pro-Lys N-terminal protein methyltransferase 1A
Short name:
NTM1A
Cleaved into the following chain:
Gene namesi
Name:Ntmt1
Synonyms:Mettl11a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1913867. Ntmt1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004232291 – 223N-terminal Xaa-Pro-Lys N-methyltransferase 1Add BLAST223
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00001192892 – 223N-terminal Xaa-Pro-Lys N-methyltransferase 1, N-terminally processedAdd BLAST222

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylthreonine; in N-terminal Xaa-Pro-Lys N-methyltransferase 1, N-terminally processedBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8R2U4.
MaxQBiQ8R2U4.
PaxDbiQ8R2U4.
PeptideAtlasiQ8R2U4.
PRIDEiQ8R2U4.

PTM databases

PhosphoSitePlusiQ8R2U4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026857.
ExpressionAtlasiQ8R2U4. baseline and differential.
GenevisibleiQ8R2U4. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035303.

Structurei

3D structure databases

ProteinModelPortaliQ8R2U4.
SMRiQ8R2U4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni91 – 93S-adenosyl-L-methionine bindingBy similarity3
Regioni119 – 120S-adenosyl-L-methionine bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3178. Eukaryota.
ENOG410XS7T. LUCA.
GeneTreeiENSGT00390000008371.
HOGENOMiHOG000161910.
HOVERGENiHBG054992.
InParanoidiQ8R2U4.
KOiK16219.
OMAiPVYMIAC.
OrthoDBiEOG091G0NA7.
PhylomeDBiQ8R2U4.
TreeFamiTF314174.

Family and domain databases

InterProiView protein in InterPro
IPR008576. MeTrfase_NTM1.
IPR029063. SAM-dependent_MTases.
PANTHERiPTHR12753. PTHR12753. 1 hit.
PfamiView protein in Pfam
PF05891. Methyltransf_PK. 1 hit.
PIRSFiPIRSF016958. DUF858_MeTrfase_lik. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R2U4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSEVIEDEK QFYSKAKTYW KQIPPTVDGM LGGYGHISNI DLNSSRKFLQ
60 70 80 90 100
RFLREGPNKT GTSCALDCGA GIGRITKRLL LPLFRVVDMV DVTEDFLAKA
110 120 130 140 150
KTYLGEEGKR VRNYFCCGLQ DFSPEPGSYD VIWIQWVIGH LTDQHLAEFL
160 170 180 190 200
RRCKRGLRPN GIIVIKDNMA QEGVILDDVD SSVCRDLEVV RRIIRTAGLS
210 220
LLAEERQENL PDEIYHVYSF ALR
Length:223
Mass (Da):25,420
Last modified:January 23, 2007 - v3
Checksum:i07822C8CB2F65CA1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK154783 mRNA. Translation: BAE32826.1.
AL844532 Genomic DNA. Translation: CAM17872.1.
CH466542 Genomic DNA. Translation: EDL08489.1.
CH466542 Genomic DNA. Translation: EDL08490.1.
BC027220 mRNA. Translation: AAH27220.1.
CCDSiCCDS15886.1.
RefSeqiNP_733480.1. NM_170592.2.
XP_006498289.1. XM_006498226.3.
UniGeneiMm.33167.

Genome annotation databases

EnsembliENSMUST00000041830; ENSMUSP00000035303; ENSMUSG00000026857.
GeneIDi66617.
KEGGimmu:66617.
UCSCiuc008jcv.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiNTM1A_MOUSE
AccessioniPrimary (citable) accession number: Q8R2U4
Secondary accession number(s): A2APZ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 120 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families