Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Actin-related protein 8

Gene

Actr8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Plays an important role in the functional organization of mitotic chromosomes. Exhibits low basal ATPase activity, and unable to polymerize (By similarity).By similarity
Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. Required for the recruitment of INO80 (and probably the INO80 complex) to sites of DNA damage Strongly prefer nucleosomes and H3-H4 tetramers over H2A-H2B dimers, suggesting it may act as a nucleosome recognition module within the complex (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei55 – 551ATPBy similarity
Binding sitei56 – 561ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi283 – 2864ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, Mitosis, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-5696394. DNA Damage Recognition in GG-NER.

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-related protein 8
Gene namesi
Name:Actr8
Synonyms:Arp8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1860775. Actr8.

Subcellular locationi

  • Nucleus By similarity
  • Chromosome By similarity

  • Note: Specifically localizes to mitotic chromosomes.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 624624Actin-related protein 8PRO_0000089124Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei132 – 1321PhosphoserineBy similarity
Modified residuei412 – 4121PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8R2S9.
MaxQBiQ8R2S9.
PaxDbiQ8R2S9.
PRIDEiQ8R2S9.

PTM databases

iPTMnetiQ8R2S9.
PhosphoSiteiQ8R2S9.

Expressioni

Gene expression databases

BgeeiQ8R2S9.
GenevisibleiQ8R2S9. MM.

Interactioni

Subunit structurei

Component of the chromatin remodeling INO80 complex; specifically part of a complex module associated with the DBINO domain of INO80. Exists as monomers and dimers, but the dimer is most probably the biologically relevant form required for stable interactions with histones that exploits the twofold symmetry of the nucleosome core (By similarity).By similarity

Protein-protein interaction databases

BioGridi207864. 1 interaction.
STRINGi10090.ENSMUSP00000016115.

Structurei

3D structure databases

ProteinModelPortaliQ8R2S9.
SMRiQ8R2S9. Positions 34-410, 424-624.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the actin family. ARP8 subfamily.Curated

Phylogenomic databases

eggNOGiKOG0797. Eukaryota.
COG5277. LUCA.
GeneTreeiENSGT00390000001763.
HOGENOMiHOG000034073.
HOVERGENiHBG023939.
InParanoidiQ8R2S9.
KOiK11673.
OMAiSIDCCAS.
OrthoDBiEOG7T1R9P.
PhylomeDBiQ8R2S9.
TreeFamiTF324575.

Family and domain databases

InterProiIPR004000. Actin.
IPR027668. Arp8/plant_Arp9.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 3 hits.
PTHR11937:SF13. PTHR11937:SF13. 3 hits.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R2S9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQAEKGDAE NGKEKGGEKE KEQRGVKRPI VPALVPESLQ EQIQSNFIVV
60 70 80 90 100
IHPGSTTLRL GRATDTLPVS VPHVIARRHK QQGQPLYKDN WLLREGLNKP
110 120 130 140 150
ESNEQRQNGL KMVDQAIWSK KMSNGTRRIP VSPEQTRSYN KQMRPAILDH
160 170 180 190 200
CSGNKWTNTS QQPEYLVGEE ALYVNPLDCY NIHWPIRRGQ LNIHPGPGGS
210 220 230 240 250
LTAVLADIEV IWSHAIQKYL EIPLKDLKYY RCILLIPDIY NKQHVKELVH
260 270 280 290 300
MILMKMGFAG IVVHQESVCA TFGSGLSSTC VVDVGDQKTS VCCVEDGVSH
310 320 330 340 350
RNTRLCLAYG GSDVSRCFYW LMQRAGFPYR ECQLTNKMDC LLLQHLKETF
360 370 380 390 400
CHLDQDISGL QDHEFQIRHP DSPALLYQFR LGDEKLQAPM ALFYPATFGI
410 420 430 440 450
VGQKMTTLQH RSQGDPEDPH DEHYLLATQS KQEQSAKATA DRKSASKPIG
460 470 480 490 500
FEGDLRGQSS DLPERLHSQE VDLASSQGDC LMAGNESEEA LTALMSRKTA
510 520 530 540 550
ISLFEGKALG LDKAILHSVD CCSSDDTKKK MYSSILVVGG GLMFHKAQEF
560 570 580 590 600
LQHRILNKMP PSFRRIIENV DVITRPKDMD PRLIAWKGGA VLACLDTTQE
610 620
LWIYQREWQR FGVRMLRERA AFVW
Length:624
Mass (Da):70,546
Last modified:June 1, 2002 - v1
Checksum:i96B79E1119C50FEA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti127 – 1271R → G in BAB30952 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017815 mRNA. Translation: BAB30952.1.
BC027281 mRNA. Translation: AAH27281.1.
CCDSiCCDS26891.1.
RefSeqiNP_081769.2. NM_027493.3.
UniGeneiMm.215110.

Genome annotation databases

EnsembliENSMUST00000016115; ENSMUSP00000016115; ENSMUSG00000015971.
GeneIDi56249.
KEGGimmu:56249.
UCSCiuc007sum.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017815 mRNA. Translation: BAB30952.1.
BC027281 mRNA. Translation: AAH27281.1.
CCDSiCCDS26891.1.
RefSeqiNP_081769.2. NM_027493.3.
UniGeneiMm.215110.

3D structure databases

ProteinModelPortaliQ8R2S9.
SMRiQ8R2S9. Positions 34-410, 424-624.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207864. 1 interaction.
STRINGi10090.ENSMUSP00000016115.

PTM databases

iPTMnetiQ8R2S9.
PhosphoSiteiQ8R2S9.

Proteomic databases

EPDiQ8R2S9.
MaxQBiQ8R2S9.
PaxDbiQ8R2S9.
PRIDEiQ8R2S9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000016115; ENSMUSP00000016115; ENSMUSG00000015971.
GeneIDi56249.
KEGGimmu:56249.
UCSCiuc007sum.2. mouse.

Organism-specific databases

CTDi93973.
MGIiMGI:1860775. Actr8.

Phylogenomic databases

eggNOGiKOG0797. Eukaryota.
COG5277. LUCA.
GeneTreeiENSGT00390000001763.
HOGENOMiHOG000034073.
HOVERGENiHBG023939.
InParanoidiQ8R2S9.
KOiK11673.
OMAiSIDCCAS.
OrthoDBiEOG7T1R9P.
PhylomeDBiQ8R2S9.
TreeFamiTF324575.

Enzyme and pathway databases

ReactomeiR-MMU-5696394. DNA Damage Recognition in GG-NER.

Miscellaneous databases

PROiQ8R2S9.
SOURCEiSearch...

Gene expression databases

BgeeiQ8R2S9.
GenevisibleiQ8R2S9. MM.

Family and domain databases

InterProiIPR004000. Actin.
IPR027668. Arp8/plant_Arp9.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 3 hits.
PTHR11937:SF13. PTHR11937:SF13. 3 hits.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "The mammalian INO80 complex is recruited to DNA damage sites in an ARP8 dependent manner."
    Kashiwaba S., Kitahashi K., Watanabe T., Onoda F., Ohtsu M., Murakami Y.
    Biochem. Biophys. Res. Commun. 402:619-625(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiARP8_MOUSE
AccessioniPrimary (citable) accession number: Q8R2S9
Secondary accession number(s): Q9CYC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: June 1, 2002
Last modified: June 8, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.