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Protein

Bone marrow stromal antigen 2

Gene

Bst2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

IFN-induced antiviral host restriction factor which efficiently blocks the release of diverse mammalian enveloped viruses by directly tethering nascent virions to the membranes of infected cells. Acts as a direct physical tether, holding virions to the cell membrane and linking virions to each other. The tethered virions can be internalized by endocytosis and subsequently degraded or they can remain on the cell surface. In either case, their spread as cell-free virions is restricted. Its target viruses belong to diverse families, including retroviridae: human immunodeficiency virus type 1 (HIV-1), mouse mammary tumor virus (MMTV) and murine leukemia virus (MLV), filoviridae: ebola virus (EBOV), arenaviridae: lassa virus (LASV), and rhabdoviridae: vesicular stomatitis virus (VSV). Can inhibit cell surface proteolytic activity of MMP14 causing decreased activation of MMP15 which results in inhibition of cell growth and migration. Can stimulate signaling by LILRA4/ILT7 and consequently provide negative feedback to the production of IFN by plasmacytoid dendritic cells in response to viral infection. Plays a role in the organization of the subapical actin cytoskeleton in polarized epithelial cells.8 Publications

GO - Molecular functioni

GO - Biological processi

  • defense response to virus Source: GO_Central
  • innate immune response Source: UniProtKB
  • negative regulation of cell growth Source: UniProtKB
  • negative regulation of cell migration Source: UniProtKB
  • negative regulation of intracellular transport of viral material Source: UniProtKB
  • negative regulation of plasmacytoid dendritic cell cytokine production Source: UniProtKB
  • negative regulation of viral genome replication Source: UniProtKB
  • positive regulation of I-kappaB kinase/NF-kappaB signaling Source: Ensembl
  • regulation of actin cytoskeleton organization Source: UniProtKB
  • response to interferon-alpha Source: UniProtKB
  • response to interferon-beta Source: UniProtKB
  • response to interferon-gamma Source: UniProtKB
  • response to virus Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Immunity, Innate immunity

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Bone marrow stromal antigen 2
Short name:
BST-2
Alternative name(s):
HM1.24 antigen
CD_antigen: CD317
Gene namesi
Name:Bst2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1916800. Bst2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 30CytoplasmicSequence analysisAdd BLAST30
Transmembranei31 – 51Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini52 – 152ExtracellularSequence analysisAdd BLAST101

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002535531 – 152Bone marrow stromal antigen 2Add BLAST152
PropeptideiPRO_0000253554153 – 172Removed in mature formSequence analysisAdd BLAST20

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi58Interchain1 Publication
Disulfide bondi68Interchain1 Publication
Glycosylationi70N-linked (GlcNAc...)1 Publication1
Glycosylationi94N-linked (GlcNAc...); atypical1 Publication1
Disulfide bondi96Interchain1 Publication
Glycosylationi97N-linked (GlcNAc...)Sequence analysis1
Lipidationi152GPI-anchor amidated serineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

EPDiQ8R2Q8.
MaxQBiQ8R2Q8.
PaxDbiQ8R2Q8.
PeptideAtlasiQ8R2Q8.
PRIDEiQ8R2Q8.

PTM databases

iPTMnetiQ8R2Q8.
PhosphoSitePlusiQ8R2Q8.
SwissPalmiQ8R2Q8.

Expressioni

Tissue specificityi

In naive mice, specifically expressed on type I interferon-producing cells (at protein level).1 Publication

Inductioni

By viral or other interferon-inducing stimulation in most cell types (at protein level). Down-regulated by ebola virus GP protein.2 Publications

Gene expression databases

BgeeiENSMUSG00000046718.
CleanExiMM_BST2.
GenevisibleiQ8R2Q8. MM.

Interactioni

Subunit structurei

Parallel homodimer; disulfide-linked. May form homotetramers under reducing conditions. Dimerization is essential for its antiviral activity. Interacts (via cytoplasmic domain) with ARHGAP44 (By similarity). Interacts with MMP14 (via C-terminal cytoplasmic tail) (By similarity). Interacts with LILRA4/ILT7 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ8R2Q8. 1 interactor.
STRINGi10090.ENSMUSP00000051921.

Structurei

Secondary structure

1172
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi59 – 143Combined sources85
Turni144 – 146Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3NI0X-ray1.60A/B53-151[»]
ProteinModelPortaliQ8R2Q8.
SMRiQ8R2Q8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8R2Q8.

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili74 – 1471 PublicationAdd BLAST74

Domaini

The extracellular coiled coil domain forms an extended 170 A long semi-flexible rod-like structure important for virion retention at the cell surface and prevention of virus spreading.By similarity

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410JE1U. Eukaryota.
ENOG41115GP. LUCA.
GeneTreeiENSGT00390000013782.
HOGENOMiHOG000013084.
HOVERGENiHBG004902.
InParanoidiQ8R2Q8.
KOiK06731.
OMAiECRNTTH.
OrthoDBiEOG091G1B7U.
PhylomeDBiQ8R2Q8.
TreeFamiTF338345.

Family and domain databases

InterProiIPR024886. BST2.
[Graphical view]
PANTHERiPTHR15190. PTHR15190. 1 hit.
PfamiPF16716. BST2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R2Q8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPSFYHYLP VPMDEMGGKQ GWGSHRQWLG AAILVVLFGV TLVILTIYFA
60 70 80 90 100
VTANSVACRD GLRAQAECRN TTHLLQRQLT RTQDSLLQAE TQANSCNLTV
110 120 130 140 150
VTLQESLEKK VSQALEQQAR IKELENEVTK LNQELENLRI QKETSSTVQV
160 170
NSGSSMVVSS LLVLKVSLFL LF
Length:172
Mass (Da):19,152
Last modified:June 1, 2002 - v1
Checksum:iA2032FD01FAF601A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK009361 mRNA. Translation: BAC25254.1.
AK154399 mRNA. Translation: BAE32560.1.
BC027328 mRNA. Translation: AAH27328.1.
BC056638 mRNA. Translation: AAH56638.1.
BC087949 mRNA. Translation: AAH87949.1.
CCDSiCCDS22397.1.
RefSeqiNP_932763.1. NM_198095.2.
UniGeneiMm.260325.

Genome annotation databases

EnsembliENSMUST00000051672; ENSMUSP00000051921; ENSMUSG00000046718.
GeneIDi69550.
KEGGimmu:69550.
UCSCiuc009mdr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK009361 mRNA. Translation: BAC25254.1.
AK154399 mRNA. Translation: BAE32560.1.
BC027328 mRNA. Translation: AAH27328.1.
BC056638 mRNA. Translation: AAH56638.1.
BC087949 mRNA. Translation: AAH87949.1.
CCDSiCCDS22397.1.
RefSeqiNP_932763.1. NM_198095.2.
UniGeneiMm.260325.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3NI0X-ray1.60A/B53-151[»]
ProteinModelPortaliQ8R2Q8.
SMRiQ8R2Q8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8R2Q8. 1 interactor.
STRINGi10090.ENSMUSP00000051921.

PTM databases

iPTMnetiQ8R2Q8.
PhosphoSitePlusiQ8R2Q8.
SwissPalmiQ8R2Q8.

Proteomic databases

EPDiQ8R2Q8.
MaxQBiQ8R2Q8.
PaxDbiQ8R2Q8.
PeptideAtlasiQ8R2Q8.
PRIDEiQ8R2Q8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000051672; ENSMUSP00000051921; ENSMUSG00000046718.
GeneIDi69550.
KEGGimmu:69550.
UCSCiuc009mdr.1. mouse.

Organism-specific databases

CTDi684.
MGIiMGI:1916800. Bst2.

Phylogenomic databases

eggNOGiENOG410JE1U. Eukaryota.
ENOG41115GP. LUCA.
GeneTreeiENSGT00390000013782.
HOGENOMiHOG000013084.
HOVERGENiHBG004902.
InParanoidiQ8R2Q8.
KOiK06731.
OMAiECRNTTH.
OrthoDBiEOG091G1B7U.
PhylomeDBiQ8R2Q8.
TreeFamiTF338345.

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

ChiTaRSiBst2. mouse.
EvolutionaryTraceiQ8R2Q8.
PROiQ8R2Q8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000046718.
CleanExiMM_BST2.
GenevisibleiQ8R2Q8. MM.

Family and domain databases

InterProiIPR024886. BST2.
[Graphical view]
PANTHERiPTHR15190. PTHR15190. 1 hit.
PfamiPF16716. BST2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBST2_MOUSE
AccessioniPrimary (citable) accession number: Q8R2Q8
Secondary accession number(s): Q8CEY7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: June 1, 2002
Last modified: November 30, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.