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Protein

Kelch-like protein 25

Gene

Klhl25

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for translational homeostasis. The BCR(KLHL25) ubiquitin ligase complex acts by mediating ubiquitination of hypophosphorylated EIF4EBP1 (4E-BP1): ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1 (4E-BP1) probably serves as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. The BCR(KLHL25) complex does not target EIF4EBP1 (4E-BP1) when it is hyperphosphorylated or associated with eIF4E.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Translation regulation, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Kelch-like protein 25
Alternative name(s):
Ectoderm-neural cortex protein 2
Short name:
ENC-2
Gene namesi
Name:Klhl25
Synonyms:Enc2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2668031. Klhl25.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 589589Kelch-like protein 25PRO_0000272309Add
BLAST

Proteomic databases

EPDiQ8R2P1.
MaxQBiQ8R2P1.
PaxDbiQ8R2P1.
PeptideAtlasiQ8R2P1.
PRIDEiQ8R2P1.

PTM databases

iPTMnetiQ8R2P1.
PhosphoSiteiQ8R2P1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000055652.
CleanExiMM_KLHL25.
GenevisibleiQ8R2P1. MM.

Interactioni

Subunit structurei

Component of the BCR(KLHL25) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL25 and RBX1. Interacts with EIF4EBP1 (when hypophosphorylated) (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000089707.

Structurei

3D structure databases

ProteinModelPortaliQ8R2P1.
SMRiQ8R2P1. Positions 3-271, 297-570.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 11469BTBPROSITE-ProRule annotationAdd
BLAST
Domaini149 – 250102BACKAdd
BLAST
Repeati296 – 34045Kelch 1Add
BLAST
Repeati341 – 38848Kelch 2Add
BLAST
Repeati389 – 44456Kelch 3Add
BLAST
Repeati446 – 49247Kelch 4Add
BLAST
Repeati493 – 53846Kelch 5Add
BLAST
Repeati539 – 58547Kelch 6Add
BLAST

Sequence similaritiesi

Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
Contains 6 Kelch repeats.Curated

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiKOG4441. Eukaryota.
ENOG410XNX8. LUCA.
GeneTreeiENSGT00840000129689.
HOGENOMiHOG000232171.
HOVERGENiHBG000905.
InParanoidiQ8R2P1.
KOiK10462.
OMAiTSDTWNC.
OrthoDBiEOG091G03JU.
PhylomeDBiQ8R2P1.
TreeFamiTF329218.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
InterProiIPR011705. BACK.
IPR017096. BTB-kelch_protein.
IPR000210. BTB/POZ_dom.
IPR015915. Kelch-typ_b-propeller.
IPR006652. Kelch_1.
IPR030565. KLHL25.
IPR011333. SKP1/BTB/POZ.
[Graphical view]
PANTHERiPTHR24410:SF10. PTHR24410:SF10. 1 hit.
PfamiPF07707. BACK. 1 hit.
PF00651. BTB. 1 hit.
PF01344. Kelch_1. 4 hits.
[Graphical view]
PIRSFiPIRSF037037. Kelch-like_protein_gigaxonin. 1 hit.
SMARTiSM00875. BACK. 1 hit.
SM00225. BTB. 1 hit.
SM00612. Kelch. 6 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R2P1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVSVHETRK SRSSTGSMNI SVFHKASHPD CVLAHLNTLR KHCMFTDVTL
60 70 80 90 100
WAGDRAFPCH RAVLAASSRY FEAMFSHGLR ESRDDTVNFQ DNLHPEVLEL
110 120 130 140 150
LLDFAYSSRI VINEENAESL LEAGDMLQFH DVRDAAAEFL EKNLSPSNCL
160 170 180 190 200
GMMVLSDAHQ CRRLYEFSCR MSLVHFETVR QSEDFNSLSR DTLLDLISRD
210 220 230 240 250
ELETEDERVV FEAILQWVKH DLEQRKAHLP LLLRNVRLAL LPSDCLKNAV
260 270 280 290 300
SGEALLMADE CTKLILDEAF RCKTKILLND GVVTSPFARP RKAGHTLLIL
310 320 330 340 350
GGQTFMCDKI YQVDHKAKEI IPKADLPSPR KEFSASAIGC KVYVTGGRGS
360 370 380 390 400
ENGVSKDVWV YDTVHEEWSK AAPMLIARFG HGSAELENCL YVVGGHTSLA
410 420 430 440 450
GIFPASPSVS LKQVEKYDPG DNKWTMVAPM RDGVSNAAVV SAKLKLFVFG
460 470 480 490 500
GTSIHRDMVS KVQCFDPSEN RWTIKAECPQ PWRYTAAAVL GSQIFIMGGD
510 520 530 540 550
TEYTAASAYR FDCETNQWTR IGDMTAKRMS CHAVASGNKL YVVGGYFGTQ
560 570 580
RCKTLDCYDP TSDTWNCITT VPYSLIPTAF VSTWKHLPA
Length:589
Mass (Da):65,826
Last modified:June 1, 2002 - v1
Checksum:i5B8C746A80287C20
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK143086 mRNA. Translation: BAE25266.1.
BC027373 mRNA. Translation: AAH27373.1.
CCDSiCCDS21369.1.
RefSeqiNP_001116252.1. NM_001122780.1.
NP_083928.1. NM_029652.1.
NP_877583.1. NM_182782.2.
XP_011249134.1. XM_011250832.1.
UniGeneiMm.206223.

Genome annotation databases

EnsembliENSMUST00000092073; ENSMUSP00000089707; ENSMUSG00000055652.
ENSMUST00000171155; ENSMUSP00000133175; ENSMUSG00000055652.
ENSMUST00000206019; ENSMUSP00000146102; ENSMUSG00000055652.
GeneIDi207952.
KEGGimmu:207952.
UCSCiuc009hwy.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK143086 mRNA. Translation: BAE25266.1.
BC027373 mRNA. Translation: AAH27373.1.
CCDSiCCDS21369.1.
RefSeqiNP_001116252.1. NM_001122780.1.
NP_083928.1. NM_029652.1.
NP_877583.1. NM_182782.2.
XP_011249134.1. XM_011250832.1.
UniGeneiMm.206223.

3D structure databases

ProteinModelPortaliQ8R2P1.
SMRiQ8R2P1. Positions 3-271, 297-570.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000089707.

PTM databases

iPTMnetiQ8R2P1.
PhosphoSiteiQ8R2P1.

Proteomic databases

EPDiQ8R2P1.
MaxQBiQ8R2P1.
PaxDbiQ8R2P1.
PeptideAtlasiQ8R2P1.
PRIDEiQ8R2P1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000092073; ENSMUSP00000089707; ENSMUSG00000055652.
ENSMUST00000171155; ENSMUSP00000133175; ENSMUSG00000055652.
ENSMUST00000206019; ENSMUSP00000146102; ENSMUSG00000055652.
GeneIDi207952.
KEGGimmu:207952.
UCSCiuc009hwy.2. mouse.

Organism-specific databases

CTDi64410.
MGIiMGI:2668031. Klhl25.

Phylogenomic databases

eggNOGiKOG4441. Eukaryota.
ENOG410XNX8. LUCA.
GeneTreeiENSGT00840000129689.
HOGENOMiHOG000232171.
HOVERGENiHBG000905.
InParanoidiQ8R2P1.
KOiK10462.
OMAiTSDTWNC.
OrthoDBiEOG091G03JU.
PhylomeDBiQ8R2P1.
TreeFamiTF329218.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ8R2P1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000055652.
CleanExiMM_KLHL25.
GenevisibleiQ8R2P1. MM.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
InterProiIPR011705. BACK.
IPR017096. BTB-kelch_protein.
IPR000210. BTB/POZ_dom.
IPR015915. Kelch-typ_b-propeller.
IPR006652. Kelch_1.
IPR030565. KLHL25.
IPR011333. SKP1/BTB/POZ.
[Graphical view]
PANTHERiPTHR24410:SF10. PTHR24410:SF10. 1 hit.
PfamiPF07707. BACK. 1 hit.
PF00651. BTB. 1 hit.
PF01344. Kelch_1. 4 hits.
[Graphical view]
PIRSFiPIRSF037037. Kelch-like_protein_gigaxonin. 1 hit.
SMARTiSM00875. BACK. 1 hit.
SM00225. BTB. 1 hit.
SM00612. Kelch. 6 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKLH25_MOUSE
AccessioniPrimary (citable) accession number: Q8R2P1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: June 1, 2002
Last modified: September 7, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.