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Q8R2N1

- AQP3_MOUSE

UniProt

Q8R2N1 - AQP3_MOUSE

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Protein

Aquaporin-3

Gene

Aqp3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism.2 Publications

GO - Molecular functioni

  1. glycerol channel activity Source: Ensembl
  2. water channel activity Source: MGI

GO - Biological processi

  1. glycerol transport Source: UniProtKB
  2. odontogenesis Source: Ensembl
  3. positive regulation of immune system process Source: Ensembl
  4. renal water absorption Source: MGI
  5. response to retinoic acid Source: Ensembl
  6. urea transport Source: MGI
  7. water transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiREACT_213947. Vasopressin regulates renal water homeostasis via Aquaporins.
REACT_259079. Passive transport by Aquaporins.

Names & Taxonomyi

Protein namesi
Recommended name:
Aquaporin-3
Short name:
AQP-3
Alternative name(s):
Aquaglyceroporin-3
Gene namesi
Name:Aqp3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:1333777. Aqp3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2828CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei29 – 4921HelicalSequence AnalysisAdd
BLAST
Topological domaini50 – 534ExtracellularSequence Analysis
Transmembranei54 – 7421HelicalSequence AnalysisAdd
BLAST
Topological domaini75 – 10935CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei110 – 13021HelicalSequence AnalysisAdd
BLAST
Topological domaini131 – 15727ExtracellularSequence AnalysisAdd
BLAST
Transmembranei158 – 17821HelicalSequence AnalysisAdd
BLAST
Topological domaini179 – 18810CytoplasmicSequence Analysis
Transmembranei189 – 20921HelicalSequence AnalysisAdd
BLAST
Topological domaini210 – 24435ExtracellularSequence AnalysisAdd
BLAST
Transmembranei245 – 26521HelicalSequence AnalysisAdd
BLAST
Topological domaini266 – 29227CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. basolateral plasma membrane Source: MGI
  2. cell-cell junction Source: MGI
  3. cytoplasm Source: Ensembl
  4. integral component of plasma membrane Source: MGI
  5. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Mice have dry skin with reduced SC (stratum corneum) hydration, decreased elasticity and impaired biosynthesis. The glycerol content of SC and epidermis is reduced, whereas that of dermis and serum is normal. The dry, relatively inelastic skin is probably related to the humectant properties of glycerol, and the impaired SC repair to impaired epidermal biosynthetic function.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 292292Aquaporin-3PRO_0000063944Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi141 – 1411N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8R2N1.
PRIDEiQ8R2N1.

Expressioni

Tissue specificityi

Renal medulla and colon. Predominantly in the inner medulla. Expressed in basal layer of epidermal keratinocytes.

Gene expression databases

BgeeiQ8R2N1.
CleanExiMM_AQP3.
GenevestigatoriQ8R2N1.

Structurei

3D structure databases

ProteinModelPortaliQ8R2N1.
SMRiQ8R2N1. Positions 24-269.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi83 – 853NPA 1
Motifi215 – 2173NPA 2

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0580.
GeneTreeiENSGT00510000046311.
HOGENOMiHOG000288287.
HOVERGENiHBG106057.
InParanoidiQ8R2N1.
KOiK09876.
OMAiLDMINGF.
OrthoDBiEOG7J9VPP.
PhylomeDBiQ8R2N1.
TreeFamiTF313173.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR023275. Aquaporin_3.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR02015. AQUAPORIN3.
PR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R2N1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGRQKELMNR CGEMLHIRYR LLRQALAECL GTLILVMFGC GSVAQVVLSR
60 70 80 90 100
GTHGGFLTIN LAFGFAVTLG ILVAGQVSGA HLNPAVTFAM CFLAREPWIK
110 120 130 140 150
LPIYALAQTL GAFLGAGIVF GLYYDAIWAF ANNELFVSGP NGTAGIFATY
160 170 180 190 200
PSGHLDMVNG FFDQFIGTAA LIVCVLAIVD PYNNPVPRGL EAFTVGLVVL
210 220 230 240 250
VIGTSMGFNS GYAVNPARDF GPRLFTALAG WGSEVFTTGR HWWWVPIVSP
260 270 280 290
LLGSIAGVFV YQLMIGCHLE QPPPSTEEEN VKLAHMKHKE QI
Length:292
Mass (Da):31,568
Last modified:June 1, 2002 - v1
Checksum:iE9A7FFFB43DD98C5
GO

Sequence cautioni

The sequence BAB03270.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti170 – 1734ALIV → PLL in AAD20304. (PubMed:12771381)Curated
Sequence conflicti170 – 1734ALIV → PLL in AAD20305. (PubMed:12771381)Curated
Sequence conflicti214 – 2141V → A in BAB03270. (PubMed:10777495)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF104416 mRNA. Translation: AAD20304.1.
AF104417 Genomic DNA. Translation: AAD20305.1.
AK145678 mRNA. Translation: BAE26584.1.
AK160929 mRNA. Translation: BAE36096.1.
AL837521 Genomic DNA. Translation: CAM19849.1.
CH466538 Genomic DNA. Translation: EDL05416.1.
BC027400 mRNA. Translation: AAH27400.1.
AB019039 mRNA. Translation: BAB03270.1. Different initiation.
CCDSiCCDS38714.1.
RefSeqiNP_057898.2. NM_016689.2.
UniGeneiMm.34043.

Genome annotation databases

EnsembliENSMUST00000055327; ENSMUSP00000055110; ENSMUSG00000028435.
GeneIDi11828.
KEGGimmu:11828.
UCSCiuc008sih.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF104416 mRNA. Translation: AAD20304.1 .
AF104417 Genomic DNA. Translation: AAD20305.1 .
AK145678 mRNA. Translation: BAE26584.1 .
AK160929 mRNA. Translation: BAE36096.1 .
AL837521 Genomic DNA. Translation: CAM19849.1 .
CH466538 Genomic DNA. Translation: EDL05416.1 .
BC027400 mRNA. Translation: AAH27400.1 .
AB019039 mRNA. Translation: BAB03270.1 . Different initiation.
CCDSi CCDS38714.1.
RefSeqi NP_057898.2. NM_016689.2.
UniGenei Mm.34043.

3D structure databases

ProteinModelPortali Q8R2N1.
SMRi Q8R2N1. Positions 24-269.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q8R2N1.
PRIDEi Q8R2N1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000055327 ; ENSMUSP00000055110 ; ENSMUSG00000028435 .
GeneIDi 11828.
KEGGi mmu:11828.
UCSCi uc008sih.1. mouse.

Organism-specific databases

CTDi 360.
MGIi MGI:1333777. Aqp3.

Phylogenomic databases

eggNOGi COG0580.
GeneTreei ENSGT00510000046311.
HOGENOMi HOG000288287.
HOVERGENi HBG106057.
InParanoidi Q8R2N1.
KOi K09876.
OMAi LDMINGF.
OrthoDBi EOG7J9VPP.
PhylomeDBi Q8R2N1.
TreeFami TF313173.

Enzyme and pathway databases

Reactomei REACT_213947. Vasopressin regulates renal water homeostasis via Aquaporins.
REACT_259079. Passive transport by Aquaporins.

Miscellaneous databases

NextBioi 279735.
PROi Q8R2N1.
SOURCEi Search...

Gene expression databases

Bgeei Q8R2N1.
CleanExi MM_AQP3.
Genevestigatori Q8R2N1.

Family and domain databases

Gene3Di 1.20.1080.10. 1 hit.
InterProi IPR023271. Aquaporin-like.
IPR023275. Aquaporin_3.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view ]
PANTHERi PTHR19139. PTHR19139. 1 hit.
Pfami PF00230. MIP. 1 hit.
[Graphical view ]
PRINTSi PR02015. AQUAPORIN3.
PR00783. MINTRINSICP.
SUPFAMi SSF81338. SSF81338. 1 hit.
TIGRFAMsi TIGR00861. MIP. 1 hit.
PROSITEi PS00221. MIP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Glycerol replacement corrects defective skin hydration, elasticity, and barrier function in aquaporin-3-deficient mice."
    Hara M., Verkman A.S.
    Proc. Natl. Acad. Sci. U.S.A. 100:7360-7365(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, DISRUPTION PHENOTYPE.
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Extraembryonic tissue and Placenta.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-292.
    Tissue: Kidney.
  7. "The role of aquaporin 3 in the movement of water and cryoprotectants in mouse morulae."
    Edashige K., Ohta S., Tanaka M., Kuwano T., Valdez D.M. Jr., Hara T., Jin B., Takahashi S., Seki S., Koshimoto C., Kasai M.
    Biol. Reprod. 77:365-375(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiAQP3_MOUSE
AccessioniPrimary (citable) accession number: Q8R2N1
Secondary accession number(s): Q3TU75, Q9JJN8, Q9WTJ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: June 1, 2002
Last modified: November 26, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3