Q8R2N1 (AQP3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aquaporin-3
Short name=AQP-3

Alternative name(s):
Aquaglyceroporin-3

Gene names

Name:

Aqp3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism. Ref.1 Ref.7
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Renal medulla and colon. Predominantly in the inner medulla. Expressed in basal layer of epidermal keratinocytes.
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Disruption phenotype	Mice have dry skin with reduced SC (stratum corneum) hydration, decreased elasticity and impaired biosynthesis. The glycerol content of SC and epidermis is reduced, whereas that of dermis and serum is normal. The dry, relatively inelastic skin is probably related to the humectant properties of glycerol, and the impaired SC repair to impaired epidermal biosynthetic function. Ref.1
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. [View classification]
Sequence caution	The sequence BAB03270.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Repeat Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	renal water absorption Inferred from mutant phenotype. Source: MGI urea transport Inferred from mutant phenotype. Source: MGI
Cellular component	basolateral plasma membrane Inferred from direct assay. Source: MGI cell-cell junction Inferred from direct assay. Source: MGI integral to plasma membrane Traceable author statement. Source: MGI
Molecular function	water channel activity Traceable author statement. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Aquaporin-3		PRO_0000063944
Regions
	Topological domain	1 – 28	28	Cytoplasmic Potential
	Transmembrane	29 – 49	21	Helical; Potential
	Topological domain	50 – 53	4	Extracellular Potential
	Transmembrane	54 – 74	21	Helical; Potential
	Topological domain	75 – 109	35	Cytoplasmic Potential
	Transmembrane	110 – 130	21	Helical; Potential
	Topological domain	131 – 157	27	Extracellular Potential
	Transmembrane	158 – 178	21	Helical; Potential
	Topological domain	179 – 188	10	Cytoplasmic Potential
	Transmembrane	189 – 209	21	Helical; Potential
	Topological domain	210 – 244	35	Extracellular Potential
	Transmembrane	245 – 265	21	Helical; Potential
	Topological domain	266 – 292	27	Cytoplasmic Potential
	Motif	83 – 85	3	NPA 1
	Motif	215 – 217	3	NPA 2
Amino acid modifications
	Glycosylation	141	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	170 – 173	4	ALIV → PLL in AAD20304. Ref.1
	Sequence conflict	170 – 173	4	ALIV → PLL in AAD20305. Ref.1
	Sequence conflict	214	1	V → A in BAB03270. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q8R2N1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: E9A7FFFB43DD98C5	FASTA	292	31,568
	10 20 30 40 50 60 MGRQKELMNR CGEMLHIRYR LLRQALAECL GTLILVMFGC GSVAQVVLSR GTHGGFLTIN 70 80 90 100 110 120 LAFGFAVTLG ILVAGQVSGA HLNPAVTFAM CFLAREPWIK LPIYALAQTL GAFLGAGIVF 130 140 150 160 170 180 GLYYDAIWAF ANNELFVSGP NGTAGIFATY PSGHLDMVNG FFDQFIGTAA LIVCVLAIVD 190 200 210 220 230 240 PYNNPVPRGL EAFTVGLVVL VIGTSMGFNS GYAVNPARDF GPRLFTALAG WGSEVFTTGR 250 260 270 280 290 HWWWVPIVSP LLGSIAGVFV YQLMIGCHLE QPPPSTEEEN VKLAHMKHKE QI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Glycerol replacement corrects defective skin hydration, elasticity, and barrier function in aquaporin-3-deficient mice." Hara M., Verkman A.S. Proc. Natl. Acad. Sci. U.S.A. 100:7360-7365(2003) [PubMed: 12771381] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, DISRUPTION PHENOTYPE. Strain: C57BL/6J.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Extraembryonic tissue and Placenta.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Aquaporin adipose, a putative glycerol channel in adipocytes." Kishida K., Kuriyama H., Funahashi T., Shimomura I., Kihara S., Ouchi N., Nishida M., Nishizawa H., Masuda M., Takahashi M., Hotta K., Nakamura T., Yamashita S., Tochino Y., Matsuzawa Y. J. Biol. Chem. 275:20896-20902(2000) [PubMed: 10777495] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-292. Tissue: Kidney.
[7]	"The role of aquaporin 3 in the movement of water and cryoprotectants in mouse morulae." Edashige K., Ohta S., Tanaka M., Kuwano T., Valdez D.M. Jr., Hara T., Jin B., Takahashi S., Seki S., Koshimoto C., Kasai M. Biol. Reprod. 77:365-375(2007) [PubMed: 17429015] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF104416 mRNA. Translation: AAD20304.1. AF104417 Genomic DNA. Translation: AAD20305.1. AK145678 mRNA. Translation: BAE26584.1. AK160929 mRNA. Translation: BAE36096.1. AL837521 Genomic DNA. Translation: CAM19849.1. CH466538 Genomic DNA. Translation: EDL05416.1. BC027400 mRNA. Translation: AAH27400.1. AB019039 mRNA. Translation: BAB03270.1. Different initiation.
IPI	IPI00153612.
RefSeq	NP_057898.2. NM_016689.2.
UniGene	Mm.34043.
3D structure databases
ProteinModelPortal	Q8R2N1.
SMR	Q8R2N1. Positions 20-269.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8R2N1.
Proteomic databases
PRIDE	Q8R2N1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000055327; ENSMUSP00000055110; ENSMUSG00000028435.
GeneID	11828.
KEGG	mmu:11828.
UCSC	uc008sih.1. mouse.
Organism-specific databases
CTD	360.
MGI	MGI:1333777. Aqp3.
Phylogenomic databases
GeneTree	ENSGT00510000046311.
HOGENOM	HBG705794.
HOVERGEN	HBG106057.
InParanoid	Q8R2N1.
OMA	PWIKLPV.
OrthoDB	EOG4XD3RR.
PhylomeDB	Q8R2N1.
Gene expression databases
ArrayExpress	Q8R2N1.
Bgee	Q8R2N1.
CleanEx	MM_AQP3.
Genevestigator	Q8R2N1.
GermOnline	ENSMUSG00000028435. Mus musculus.
Family and domain databases
InterPro	IPR012269. Aquaporin. IPR023271. Aquaporin-like. IPR023275. Aquaporin_3. IPR000425. MIP. IPR022357. MIP_CS. [Graphical view]
Gene3D	G3DSA:1.20.1080.10. MIP. 1 hit.
KO	K09876.
PANTHER	PTHR19139. MIP. 1 hit.
Pfam	PF00230. MIP. 1 hit. [Graphical view]
PRINTS	PR02015. AQUAPORIN3. PR00783. MINTRINSICP.
SUPFAM	SSF81338. MIP. 1 hit.
TIGRFAMs	TIGR00861. MIP. 1 hit.
PROSITE	PS00221. MIP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	279735.
SOURCE	Search...

Entry information

Entry name

AQP3_MOUSE

Accession

Primary (citable) accession number: Q8R2N1
Secondary accession number(s): Q3TU75, Q9JJN8, Q9WTJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	June 1, 2002
Last modified:	November 16, 2011
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program