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Q8R2N1

- AQP3_MOUSE

UniProt

Q8R2N1 - AQP3_MOUSE

Protein

Aquaporin-3

Gene

Aqp3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism.2 Publications

    GO - Molecular functioni

    1. glycerol channel activity Source: Ensembl
    2. water channel activity Source: MGI

    GO - Biological processi

    1. glycerol transport Source: UniProtKB
    2. odontogenesis Source: Ensembl
    3. positive regulation of immune system process Source: Ensembl
    4. renal water absorption Source: MGI
    5. response to retinoic acid Source: Ensembl
    6. urea transport Source: MGI
    7. water transport Source: UniProtKB

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    ReactomeiREACT_213947. Regulation of water balance by renal Aquaporins.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aquaporin-3
    Short name:
    AQP-3
    Alternative name(s):
    Aquaglyceroporin-3
    Gene namesi
    Name:Aqp3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:1333777. Aqp3.

    Subcellular locationi

    GO - Cellular componenti

    1. basolateral plasma membrane Source: MGI
    2. cell-cell junction Source: MGI
    3. cytoplasm Source: Ensembl
    4. integral component of plasma membrane Source: MGI
    5. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mice have dry skin with reduced SC (stratum corneum) hydration, decreased elasticity and impaired biosynthesis. The glycerol content of SC and epidermis is reduced, whereas that of dermis and serum is normal. The dry, relatively inelastic skin is probably related to the humectant properties of glycerol, and the impaired SC repair to impaired epidermal biosynthetic function.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 292292Aquaporin-3PRO_0000063944Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi141 – 1411N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ8R2N1.
    PRIDEiQ8R2N1.

    Expressioni

    Tissue specificityi

    Renal medulla and colon. Predominantly in the inner medulla. Expressed in basal layer of epidermal keratinocytes.

    Gene expression databases

    BgeeiQ8R2N1.
    CleanExiMM_AQP3.
    GenevestigatoriQ8R2N1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8R2N1.
    SMRiQ8R2N1. Positions 24-269.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2828CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini50 – 534ExtracellularSequence Analysis
    Topological domaini75 – 10935CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini131 – 15727ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini179 – 18810CytoplasmicSequence Analysis
    Topological domaini210 – 24435ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini266 – 29227CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei29 – 4921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei54 – 7421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei110 – 13021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei158 – 17821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei189 – 20921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei245 – 26521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi83 – 853NPA 1
    Motifi215 – 2173NPA 2

    Domaini

    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

    Sequence similaritiesi

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0580.
    GeneTreeiENSGT00510000046311.
    HOGENOMiHOG000288287.
    HOVERGENiHBG106057.
    InParanoidiQ3TU75.
    KOiK09876.
    OMAiLDMINGF.
    OrthoDBiEOG7J9VPP.
    PhylomeDBiQ8R2N1.
    TreeFamiTF313173.

    Family and domain databases

    Gene3Di1.20.1080.10. 1 hit.
    InterProiIPR023271. Aquaporin-like.
    IPR023275. Aquaporin_3.
    IPR000425. MIP.
    IPR022357. MIP_CS.
    [Graphical view]
    PANTHERiPTHR19139. PTHR19139. 1 hit.
    PfamiPF00230. MIP. 1 hit.
    [Graphical view]
    PRINTSiPR02015. AQUAPORIN3.
    PR00783. MINTRINSICP.
    SUPFAMiSSF81338. SSF81338. 1 hit.
    TIGRFAMsiTIGR00861. MIP. 1 hit.
    PROSITEiPS00221. MIP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8R2N1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGRQKELMNR CGEMLHIRYR LLRQALAECL GTLILVMFGC GSVAQVVLSR    50
    GTHGGFLTIN LAFGFAVTLG ILVAGQVSGA HLNPAVTFAM CFLAREPWIK 100
    LPIYALAQTL GAFLGAGIVF GLYYDAIWAF ANNELFVSGP NGTAGIFATY 150
    PSGHLDMVNG FFDQFIGTAA LIVCVLAIVD PYNNPVPRGL EAFTVGLVVL 200
    VIGTSMGFNS GYAVNPARDF GPRLFTALAG WGSEVFTTGR HWWWVPIVSP 250
    LLGSIAGVFV YQLMIGCHLE QPPPSTEEEN VKLAHMKHKE QI 292
    Length:292
    Mass (Da):31,568
    Last modified:June 1, 2002 - v1
    Checksum:iE9A7FFFB43DD98C5
    GO

    Sequence cautioni

    The sequence BAB03270.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti170 – 1734ALIV → PLL in AAD20304. (PubMed:12771381)Curated
    Sequence conflicti170 – 1734ALIV → PLL in AAD20305. (PubMed:12771381)Curated
    Sequence conflicti214 – 2141V → A in BAB03270. (PubMed:10777495)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF104416 mRNA. Translation: AAD20304.1.
    AF104417 Genomic DNA. Translation: AAD20305.1.
    AK145678 mRNA. Translation: BAE26584.1.
    AK160929 mRNA. Translation: BAE36096.1.
    AL837521 Genomic DNA. Translation: CAM19849.1.
    CH466538 Genomic DNA. Translation: EDL05416.1.
    BC027400 mRNA. Translation: AAH27400.1.
    AB019039 mRNA. Translation: BAB03270.1. Different initiation.
    CCDSiCCDS38714.1.
    RefSeqiNP_057898.2. NM_016689.2.
    UniGeneiMm.34043.

    Genome annotation databases

    EnsembliENSMUST00000055327; ENSMUSP00000055110; ENSMUSG00000028435.
    GeneIDi11828.
    KEGGimmu:11828.
    UCSCiuc008sih.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF104416 mRNA. Translation: AAD20304.1 .
    AF104417 Genomic DNA. Translation: AAD20305.1 .
    AK145678 mRNA. Translation: BAE26584.1 .
    AK160929 mRNA. Translation: BAE36096.1 .
    AL837521 Genomic DNA. Translation: CAM19849.1 .
    CH466538 Genomic DNA. Translation: EDL05416.1 .
    BC027400 mRNA. Translation: AAH27400.1 .
    AB019039 mRNA. Translation: BAB03270.1 . Different initiation.
    CCDSi CCDS38714.1.
    RefSeqi NP_057898.2. NM_016689.2.
    UniGenei Mm.34043.

    3D structure databases

    ProteinModelPortali Q8R2N1.
    SMRi Q8R2N1. Positions 24-269.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q8R2N1.
    PRIDEi Q8R2N1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000055327 ; ENSMUSP00000055110 ; ENSMUSG00000028435 .
    GeneIDi 11828.
    KEGGi mmu:11828.
    UCSCi uc008sih.1. mouse.

    Organism-specific databases

    CTDi 360.
    MGIi MGI:1333777. Aqp3.

    Phylogenomic databases

    eggNOGi COG0580.
    GeneTreei ENSGT00510000046311.
    HOGENOMi HOG000288287.
    HOVERGENi HBG106057.
    InParanoidi Q3TU75.
    KOi K09876.
    OMAi LDMINGF.
    OrthoDBi EOG7J9VPP.
    PhylomeDBi Q8R2N1.
    TreeFami TF313173.

    Enzyme and pathway databases

    Reactomei REACT_213947. Regulation of water balance by renal Aquaporins.

    Miscellaneous databases

    NextBioi 279735.
    PROi Q8R2N1.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8R2N1.
    CleanExi MM_AQP3.
    Genevestigatori Q8R2N1.

    Family and domain databases

    Gene3Di 1.20.1080.10. 1 hit.
    InterProi IPR023271. Aquaporin-like.
    IPR023275. Aquaporin_3.
    IPR000425. MIP.
    IPR022357. MIP_CS.
    [Graphical view ]
    PANTHERi PTHR19139. PTHR19139. 1 hit.
    Pfami PF00230. MIP. 1 hit.
    [Graphical view ]
    PRINTSi PR02015. AQUAPORIN3.
    PR00783. MINTRINSICP.
    SUPFAMi SSF81338. SSF81338. 1 hit.
    TIGRFAMsi TIGR00861. MIP. 1 hit.
    PROSITEi PS00221. MIP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Glycerol replacement corrects defective skin hydration, elasticity, and barrier function in aquaporin-3-deficient mice."
      Hara M., Verkman A.S.
      Proc. Natl. Acad. Sci. U.S.A. 100:7360-7365(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, DISRUPTION PHENOTYPE.
      Strain: C57BL/6J.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Extraembryonic tissue and Placenta.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-292.
      Tissue: Kidney.
    7. "The role of aquaporin 3 in the movement of water and cryoprotectants in mouse morulae."
      Edashige K., Ohta S., Tanaka M., Kuwano T., Valdez D.M. Jr., Hara T., Jin B., Takahashi S., Seki S., Koshimoto C., Kasai M.
      Biol. Reprod. 77:365-375(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiAQP3_MOUSE
    AccessioniPrimary (citable) accession number: Q8R2N1
    Secondary accession number(s): Q3TU75, Q9JJN8, Q9WTJ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3